Growth-controlling complex, method for production thereof, preparation containing the same and uses in agriculture

FIELD: agriculture.

SUBSTANCE: air-dried raw material from terraneous parts of Echinacea purpurea plants is extracted with 90-95 % ethanol in ethanol/raw material ratio of 59-61:1, respectively. Extract is acidified with acid to obtain pH not more than 5.0. Extract is purified with polymeric sorbent and concentrated by vaporization. Obtained plant growth controlling agent contains chicory, chlorogenic and caffeic acids. Growth-controlling preparation contains growth-controlling complex in amount of 0.009-0.11 mg/ml and oxyethylated alkylphenol surfactant in ratio of 1:1. Claimed complex is useful in plant and scrub treatment and in seed soak.

EFFECT: preparation useful as immunomodulator and antistress agent.

6 cl, 6 ex

 

The present invention relates to plant growth regulators used in plant breeding to improve the quality of seed through the activation of germination and gain vigor, enhance adaptive properties and increase crop yields, stimulation corporatively processes in plants, including ornamental direction for the induction of flowering, and increased resistance to phytopathogens.

The plant growth regulators are chemical drugs that affect the processes of growth and development of plants. Used to accelerate ripening, increasing productivity and quality of crops; raising them to frost, drought and salt tolerance; combat lodging of cereals, flax and pulling seedlings vegetable; inhibit the growth of young shoots and development of axillary buds to increase the intensity of fruiting of some fruit and berry crops; the prevention of pre-harvest fruit drop; prevent germination of tubers and root crops during their long storage; accelerate the rooting of vegetative reproduction; induction of flowering agricultural and ornamental crops and in certain other cases.

As plant growth regulators in agricultural practice is widely used in mining the form of various chemical substances, related to different classes of organic compounds. This is, for example, sodium salt of benzoylpropionic acid used to improve the field germination of cotton seeds, increase the yield by treatment of seeds before sowing of 0.001% solution; α-naphthyloxy acid (alpha-NUS), used for spraying seedlings of tobacco to a sample of 0.001-0,0015% solution to stimulate the formation of roots; combined product composed of a mixture of Estepona and chloraminated used to combat the lodging of winter wheat and rye by spraying plants in the phase of troublevania; drug Ketoslim, representing a mixture of sodium salts of benzene(mono)diketogulonic acids used for use on cotton in order to increase the yield by moistening the seeds of 0.005% solution; Nician - a mixture of growth substances and vitamins: metabolic products of symbiotic fungus buckthorn, including vitamins a1And2B1In2, Biotin, Niacin, Pantothenic acid, gibberellin A1-A3, methylene blue and toluene; increases productivity and resistance to diseases (Handbook edited Vigarano and other Pesticides, M.: Agropromizdat, 1992, s-279). As follows from the above, the plant growth regulators (herbicides, retardants and others) differ between the battle toxicity, selectivity of action on certain types of plants. A large variety of them (plant growth regulators) election is determined by their action on certain types of crops (tobacco, cotton, wheat, rye, rice, flax, vegetables, fruit plants, ornamental plants), and also by their influence on the yield, improve the technological properties of them, increase immunity to disease.

In particular, a known drug Farolan, W” [2-(2-furyl)-1,3-dioxolane] as a plant growth regulator used for fruit crops (peach, plum, cherry), cereals (rice, wheat), vegetable crops (sugar beet), improving their productivity, increase their size, increased resistance to lodging, resistance to disease (Handbook of pesticides and agrochemicals permitted for use in the Russian Federation”, M: Publishing house “Agrorus”, 1999, p.178-179).

A sharp reduction in current levels of supply to the country's agriculture means the intensification of machinery, fertilizers, pesticides, and natural climate change requires significant strengthening of work on the biological function of agricultural production, including the use of technology phytohormonal regulation of production processes in agriculture should be one of the most important places.

theory of hormonal reg is acii plants has almost a century of history - from the first morpho-physiological experiments Cold Venta to modern ideas about the hormonal status of the plant and its genetic determination. Recent discoveries in this field are related to the detection of plants of the natural hormones - auxins, gibberellins, cytokinins, abscisic acid. And yet the scale of production and application of phytoregulators in the world and in our country has not achieved the expected values.

The technical task of the present invention is the creation of a new plant growth regulator, which has an immunomodulatory, anti-stress action.

The goal of the project is achieved by the fact that the new regulator (stimulator) plant growth is a complex on the basis of hydroxycinnamic acids, including tikolevu acid, chlorogenic acid and caffeic acid in a mass ratio of their 79-81:14-16:4-6, respectively, obtained by sequential processing of air-dried plant material, obtained from the aerial parts of the plant Echinacea purpurea, including extraction of raw materials 90-96%ethanol at a mass ratio of ethanol and raw materials, respectively 59-61:1, followed by acidification of the extract salicylic acid to a pH of not more than 5.0, the separation of the extract of plant inhibitors by further purification of his polymer the adsorbent and the concentration of the extract by evaporation.

The goal of the project is also achieved astragulus drug, including the above rostregulirushchego complex on the basis of hydroxycinnamic acid in a specific ratio, obtained as described above, one of the claimed group of inventions with its content in the product 0,009 - 0.11 mg/ml, and surfactant based on ethoxylated ALKYLPHENOLS with a ratio of 1:1.

The goal of the project is achieved in that the above rostregulirushchego the drug is used for processing of agricultural, ornamental, woody plants and shrubs.

To increase seed germination and increase the energy of germination the drug make in the amount of 0.2 - 1.0 mg/ton. To increase the resistance of plants to adverse environmental conditions, improve root formation and increase crop yields with this drug (taken at the rate of 0.1 - 10 mg/ha) sprayed plants in the period from full shoots before budding.

Declared as one of the inventions rostregulirushchego complex based hydroxycinnamic acid (cichorieae, chlorogenic, coffee) performs numerous functions in the life plant cells, the importance of which in some areas is opostavka role of phytohormones. In the literature there are data about the participation of chlorogenic and caffeic acids in the processes of rhizogenesis (Collection edited Mntanami, Lavrenkovoj “Natural hormones and inhibitors”, 1978, s-32). However, the results of these authors are provisional due to their pronounced dependence on the type of culture and physiological background of the study plants. In addition, the efficiency and direction of action of the drug depends on the concentration used in the set of specific acids, as well as of the concentration in the preparation.

And this feature of the actions of representatives hydroxycitric acids requires further research to determine the nature and mechanism of their action, selection of optimal conditions, including the range of sensitive crops exhibiting appropriate responsiveness to used GRC (hydroxycotinine acid). Models of systems of animals were obtained numerous results indicate the presence of the representatives of the UCC are cichorieae, chlorogenic and caffeic acids - immunomodulatory, antibacterial, antifungal and antitumor activity (in a different extent for each type of acid). Moreover, cichorieae acid, the main component of the active substance declared the drug has a potent anti-Christ. virusnoi activity implemented through the inhibition of integrase virus (Minsupala “Phenolic compounds, distribution, metabolism and function in plants”, M: Nauka, 1993, p.66-67; Vieceli “Natural growth inhibitors and phytohormones”, M: Nauka, 1974, p. 24, 93-101).

However, currently no data about similar action cichorieae acid on plant viruses. There is also no direct evidence of having considered GRC direct protective function against plant pathogens. Nevertheless, available information in the above-mentioned monographs concerning the relationship between derivatives hydroxycortisol acid and the activity of IAA-oxidase, an enzyme which plays a key role in the growth processes of plant cells, as well as data about significant changes in the composition and content of phenol carbonic compounds (CACS) in the transition of cells to the generative condition and possible connection with the transition to flowering allow to consider these representatives of the class hydroxycinnamic acids from the point of view of the prospects of their use as tools, which are able to modulate in the right direction the physiological basis of growth and generative processes in plants. And at the same time, the current situation crop practices in the face of increasing media ecologists the definition of pollution, impoverishment of the soil and weakening in this regard, the protective properties of the plant puts on the agenda the search for new tools to enhance the adaptive mechanisms of plant cells, increasing their tolerance to adverse environmental conditions, productivity growth, and in ornamental gardening in combination with improved decorative properties cultures.

Description of the invention

The active ingredient or active ingredient (A.I.) of the preparation according to the invention, presents a mixture of hydroxycitric acids (cichorieae acid : chlorogenic acid caffeic acid in a mass ratio of their, for example, 80:15:5)emitted from plant material aerial parts of Echinacea purpurea. Below is a description of a preferred variant embodiment of the invention of the claimed group relating to method of obtaining a new drug.

I. receiving the drug.

The procedure of getting a drug involves the following stages: selection of active ingredient (A.I.) and the creation of the preparative form. In turn, the stage selection A.I consists of two podstawy: primary processing of raw materials and fractionation of the extract. The specified manufacturing process includes the extraction of natural ingredients from plant source and, accordingly, is not associated with the implementation of the process of chemical transformation.

Circuits which receive the drug (complex)

I. Receiving active ingredient (A.I preparation)

One plant material to highlight the complex hydroxycitric acids and primary processing of raw materials

I.2. The fixed extraction of raw materials polar solvent

I.3. Fractionation with the aim of separating from vegetable inhibitors

I.4. Evaporation partially purified enriched A.I extract

II. Obtaining technical product complex

I. Detailed description of the stages of a preferred method of obtaining A.I rostregulirushchego complex

I.1. Obtaining plant material to highlight the complex hydroxycitric acids and primary processing of raw materials

Clinics of raw materials receive from the all-Russian Institute of medicinal and aromatic plants (VILAR). The aboveground part of the plant Echinacea purpurea hand-harvested in the second year of plant development. Supplied for the use of raw materials accompanied by a quality certificate, in which the supplier (VILAR) indicates the total content of hydroxycitric acids in dried raw materials (not less than 2.5%). It should be noted that raw Echinacea, intended for medical purposes in the form of pharmaceutical preparations must meet even higher requirements, which a number of suppliers (USA, Australia and others) certify not only on total sod is the neigh hydroxycinnamic acids, but directly on the level of the main component - cichorieae acid (0,81%).

I.2. The fixed extraction of raw materials polar solvent

The extraction is carried out dry-air material 96% ethanol (ratio of plant material and ethanol, for example, 1:60). The specified ratio of the extracted material and ethanol differs from the traditional practice, which focused on the use of 50 parts ethanol to 1 part of plant material. This modification is the result of experimental selection of optimal methods of extraction components D.V. (estimated by the amount of chlorogenic acid in the extract).

Next, the extract is decanted and add 50 mm salicylic acid for acidification of the extract (max up to pH 4-5) with the aim of strengthening the stability of the active complex (primarily cichorieae, and chlorogenic acids) and a more complete extraction of all three components A.I

Describes the mode of extraction (including extracting agent, the pH value and nature of acidifying agent) selected on the basis of studying and testing different methods for the extraction of interesting complex GRC and is optimal for the purpose of the present invention, representing one aspect of it.

Thus, the first stage receiving A.I. per 1 kg of raw material is sportsouth 60 l of ethyl alcohol and salicylic acid in the amount of 414 g (6,9 g/l) taken on a number of raw materials.

When carrying out the extraction in accordance with the described procedure cannot adequately be extracted from plant materials complex compounds of phenolic nature, however, extracted a lot of by-products, including admixtures of compounds inhibitory nature. This, of course, is its negative side and puts on the agenda the task of partial purification of the obtained extract. In this context it should be noted that contradictory literature data on the effects of SCC on the formation of roots and plant growth may be as a way of explaining the fact that these authors in the allocation of the complex or specific representatives of the GRC does not get rid of phenolic compounds inhibitory nature, and depending on the terms of the allocation of the level of such compounds and their correlation with the compounds stimulating character can change that, accordingly, affects the activity of the obtained extract. The authors aimed at reducing the negative impact of such compounds introduced a special stage of purification of the extract polymeric sorbent, which allows the separation of an activator complex cells and inhibitors of phenolic nature, which was previously a serious problem in connection with the similar behavior of these compounds in the frame is x different well-known in this field procedures for isolation and purification of GRC.

I.3. Fractionation with the aim of separating from inhibitors

For the reduction obtained in the ethanol extract of the level of impurities, primarily inhibitory effect, the authors developed a method for partial purification acidified to pH 4.5 extract fractionation on a polymeric sorbent. As a polymeric sorbent use different sorbents, such as crosslinked (co)polymers such as a copolymer of styrene with divinylbenzene (Polisorb), polyvinylpyrrolidone, and others. The method of the present invention allows cleaning in the format of a once-procedure in which impurities, including connections to inhibitory nature, remain in the liquid phase, and the desired complex GRC is deposited on polymeric sorbent (according to the spectrophotometric evaluation deposited on polymeric sorbent tap active complex - chlorogenic acid).

The elution spend 10 l of ethanol per 1 kg of recovered raw materials.

The option of using 96% ethanol at this stage of receiving active drug is fundamental, since, as follows from the several reports in the literature, cichorieae acid is sensitive to water content and high humidity subjected to accelerated degradation. Some authors recommend the use of high ethanol concentration to increase stability, in General, different GRC, still, in particular, chlorogenic, which is characterized by instability during storage. For example, the preparation of chlorogenic acid, supplied by Fluka Chemica-Biochimica (cat no.25700), is governed by the water content is not more than 3%, and enriched cichorieae acid drug Echinacea company Hammer Pharma is also limited by the water content is not more than 5%.

In addition, it is essential that cichorieae acid is poorly soluble in water at room temperature (0.5% solubility). And this fact additionally indicates the desirability of using for desorption of the complex GRC ethanol concentration.

I.4. Evaporation partially purified enriched A.I extract

The extract after stage desorption procedure partial fractionation is evaporated, for example, in vacuum on a rotary film evaporator R-500 company Buche to 1/10 of the volume.

II. Obtaining technical product (Rostregulirushchego drug)

Technical product is obtained when combining ethanol solution D.V. (96% ethanol) at a concentration of 0.01-0.1 mg/ml with media formulation of surfactant - monoalkylphenol esters of polyethylene glycol, for example, in a 1:1 ratio.

Use of the preparation according to the invention for processing plants

The preparation according to the invention may be used in quantities of from 0.1 to 10 mg/ha, the exact dose is determined is by the specific use and specific cultures, to:

strengthening adaptive properties, increase crop yields: the processing is repeated spraying (2 times) vegetative plants in the phase of full shoots - the beginning of budding work with an aqueous solution (1 ml product in 10 l water) in an amount of from 0.1 to 10 mg/ha;

stimulation corporatively processes in plants when soaking cuttings in the solution according to the invention (1 ml of product in 1 l of water) for a period of 10-24 hours;

induction of flowering: a) globalwave soaked in the working solution (1 ml of product in 1 l of water); b) vegetative plants are sprayed with a working solution (0.1 to 10 mg/ha); in this case, the preparation according to the invention accelerates the beginning of flowering ornamental plants and increases the yield of luxury products;

increase germination and increase the energy of germination by soaking the seeds at room temperature for 8-16 hours in the working solution (0.2 mg/t).

The following examples illustrate the technical result when using rostregulirushchego of the drug on the basis of hydroxycinnamic acid, obtained in a certain way, make up the group of inventions.

Example 1

For example, it was investigated the effect of the drug on the damaging seedlings of Scots pine mushroom Heterobasidium annosum (will excite the l root rot of conifers). Pine seeds, pre-soaked (20 h) in water (control) and the solution according to the invention in a concentration of 0.1 mg A.I 25 l, infected by the filtrate of the fungus. After 10 days of germination not treated seeds were infected growing strain of the fungus 94%, slow-growing by 72%. Seed treatment with the preparation according to the invention have improved stability: the number of infected pine seedlings decreased: with growing strain decreased in 2 times, and 9 times with a slow-growing strain.

Example 2

We studied the effect of seed treatment with cucumbers, tomatoes, sweet pepper preparation according to the invention on the growth and morphogenetic processes. Treatment of cucumber seeds (1.25 mg/kg/ 1.5 l water) had an impact on the intensity of germination and germination rate of seeds, length of roots and sprouts, as well as their weight. Increase yield of cucumber was 24.9%, increase tomato yield of 34%. The drug stimulates the growth of pepper plants, the process of lesoobrazovaniya and dry matter content, promotes the formation of larger size and weight of fruits (length of the fetus and 7.6 - 9.4 cm (control - 6.5 cm), diameter of fruit - 4,4 - 5,3 cm (control - 3.9 cm), fruit weight - 152,6 g (control - 130 g).

Thus, rostregulirushchego complex according to the claimed invention and rostregulirushchego the drug based on it activates the synthesis of chlorophyll, growth and RISO is enesa plants, compensate for the deficiency of natural growth regulators, enhance adaptive capacity of the organism in adverse conditions, serve as an inducer of flowering plants, are mediated antifungal and antibacterial activity.

Example 3

The preparation according to the invention showed good results when used for treatment of seeds of pea (0.4 mg/t), and for the spraying of crops (0.1 mg/ha) during budding and early flowering.

The results show the presence of a growth-stimulating action, which is manifested by an increase in length (to 34.7%) and weight of seedlings (up to 31.5%), plant height at early stages of development (up 16-31%). Registered a decrease in the intensity of development of root rot in crops of peas up to 20%. The yield of peas increased by 7.9-11.0 per cent. Treatment of seeds of spring wheat and spring barley (1 mg/t) and subsequent spraying at tillering stage (2 mg/ha) stimulated the passage of the main phases of crop development: elongation, heading, milk and dough stage, which occurred 10 days earlier than in control. Double use of the drug (seed treatment and spraying of vegetating crops) increased the grain yield of wheat from 24.9-30.0 t/ha, barley from 23.6 to 27.0 kg/ha. the Drug had a positive effect on grain quality: reduced humidity in the period UB the RCTs, increased protein content, and grain wheat and gluten. Prevalence of root crops rot in the use of the drug was reduced from 2 to 1 point.

Example 4

The ornamental plants of different taxa in the processing of the preparation according to the invention were investigated processes of formation of roots in cuttings, flowering and fruiting. Soaking trudnoiskorenyaemyh cuttings (0.2 mg/l, control - water, 14 hours) Sakura Japanese, almonds, three-bladed, roses group floribunda and hybrid tea, clematis stimulated root formation from 30 to 50%. Double treatment drug vegetative plants of nails (0.1 mg/l) beginning of budding contributed to the acceleration of plant development. Full (100%) flowering on these plots occurred by June 30, while in the control variant by this time was only 27% of flowering plants. The varieties of roses Starina after spraying flowering occurred 10 days earlier than in control. The number of buds and flowers exceeded more than 3 times.

Example 5

The preparation according to the invention has accelerated the growth of the roots and aerial parts of seedlings of Siberian larch and Scots pine in the spray at a dose of 0.001 mg/L. Treatment of seedlings of Siberian cedar has increased as the height of the seedling, and the accumulation of biomass. Experienced seedlings had indicators of height, 17-26% in excess to ntrol, and the weight of their aerial parts (stem instead of needles) exceeded the control by 19-34%. Spraying trees cherry (0.01 mg/l) drug in a budding increased yield from 7% to 28% for grade Lebedyansky and from 8% to 16% grade Kharitonov. The fruits of the cherries in the advanced options fully matured, the dropping of the ovaries was minimal.

Example 6

Spraying black currant bushes (4 mg/ha) in the budding phase of the preparation according to the invention have increased yield by 0,15-0,40 kg per Bush and reduced damage from mildew on 33-37%, Septoria leaf blotch on 19 to 42%.

Along with astragaloside properties of the product according to the invention exhibits immunomodulatory and anti-stress action. The use of the drug dramatically reduces the extent of the lesion diseases such as late blight, early blight, stem canker, disease, scab, bacteriosis, Fusarium, leaf rust, grey mould of strawberry, white rot of carrot, powdery mildew of roses and black currant. For example, treatment of winter wheat with the drug reduces the incidence of crops mildew to 0%, brown rust from 52.3% to 4%; when growing cucumbers in a secure and outdoors the drug reduces the incidence of disease by 20-60%. It is established that the drug has an adaptogenic effect on the plants in drought conditions. So, biomass Alpine Hedysarum, printing handling the data preparation, was 53 mg vs 35 mg per plant in the control variant. In wheat plants treated with the drug, with the drought increased photosynthetic capacity, was stimulated by the work unit assimilatory surface. Value CPF increased to 0.30 to 0.41 mg/cm2a day in the irrigated variant and from 0.37 to 0.52 mg/cm2a day in drought conditions, which determined the activation of accumulation of biomass in the processing of the drug and increase plant tolerance to drought.

1. A method of obtaining a regulator of growth and development of plants in the form of a complex hydroxycinnamic acid by sequential processing of air-dried plant materials from the aerial parts of the plant Echinacea purpurea, including extraction of its 90-96%benchmark when the ratio of ethanol and raw materials 59-61:1, respectively, followed by acidification of the extract salicylic acid to a pH of not more than 5.0, the separation of the extract fractions of plant inhibitors by further purification of the polymer sorbent and concentration of the extract by evaporation.

2. The regulator of the growth and development of plants, representing a complex of hydroxycinnamic acids, including tikolevu acid, chlorogenic acid and caffeic acid in a mass ratio of their respective 79-81:14-16:4-6, obtained by the method according to claim 1.

3. Rostregulirushchego ven the rat, including rostregulirushchego complex according to claim 2 with its concentration in the preparation 0,009-0.11 mg/ml and surfactant based on ethoxylated alkylphenol at a ratio of 1:1.

4. The use of a mixture of complex hydroxycinnamic acids, including tikolevu acid, chlorogenic acid and caffeic acid in a mass ratio of their respective 70-81:14-16:4-6, obtained by the method according to claim 1, with a concentration in the mixture 0,009-0.11 mg/ml and surfactants based on ethoxylated alkylphenol when the mixing ratio 1:1 as rostregulirushchego of the drug in the processing of agricultural ornamental, woody plants and shrubs.

5. The use of a mixture of complex hydroxycinnamic acids, including tikolevu acid, chlorogenic acid and caffeic acid in a mass ratio of their respective 70-81:14-16:4-6, obtained by the method according to claim 1, with a concentration in the mixture 0,009-0.11 mg/ml and surfactants based on ethoxylated alkylphenol when the mixing ratio 1: 1 as rostregulirushchego of drug deposited in the environment for seed soaking in the amount of 0.2-1.0 mg/I.

6. The use according to claim 4, characterized in that the mixture used for foliar spraying in an amount of 0.1-10 mg/ha



 

Same patents:

FIELD: organic chemistry, agriculture, herbicide composition.

SUBSTANCE: invention relates to herbicide composition, containing conventional inert additives and mixture of a) herbicidically effective amount of substance satisfying the formula I [in formula R1 and R3 are the same or different C1-C4-alkyl; R4 and R5 together form groups of formulae: -C-R6(R7)-O-C-R8(R9)-C-R10(R11)-C-R12(R13)-(Z1), -C-R14(R15)-C-R16(R17)-O-C-R18(R19)-C-R20(R21)-(Z2), or -C-R22(R23)-C-R24(R25)-C-R26(R27)-O-C-R28(R29)-(Z3), wherein each R6-R29 is hydrogen; G is hydrogen or -C(X2)-X3-R31; X2 and X3 independently are oxygen; R31 is C1-C10-alkyl]; b) herbicidic synergic amount of at least one herbicide selected from group containing sulfonylureas, phenoxyacetic acids, as well as florsulam, tralcoxidim, klodinafol-propargil, phenoxaprop-P-ethyl, trifluramine, pendimethaline, picolinafen, etc. Composition also may contain safety effective amount of protective agent, such as chloquintocet-mexyl and additive (e.g., mineral oil or C8-C22-fat acid alkyl esters) in amount of 0-2 mass %. Also disclosed is method for selective controlling of weeds and grassy plants in cultural plants by treatment of cultural plants, seeds or seedlings thereof, or vegetation area thereof with claimed composition.

EFFECT: effective composition and method for weed controlling.

5 cl, 11 tbl, 7 ex

FIELD: agriculture, in particular herbicide compositions.

SUBSTANCE: invention relates to weed controlling method for tolerant grain crops using (A) road spectrum herbicides selected from group (A1) glufosinate (salt) and related compounds; (A2) glyphosate (salt) and related compounds; and (B) one or more herbicides selected from group containing (B1) herbicides selectively effecting in grain crops especially against monocotyledonous weeds effecting on folia and/or soil (residual benefit); or (B2) herbicides selectively effecting in grain crops against monocotyledonous or dicot weeds effecting especially on folia; or (B3) herbicides selectively effecting in grain crops against monocotyledonous or dicot weeds effecting on folia or soil; or (B4) herbicides selectively effecting in grain crops against monocotyledonous or dicot weeds effecting on folia; wherein components (A) and (B) are used in synergic ration. Also are described herbicide compositions containing (A1) ) glufosinate (salt) and related compounds and herbicide from group (B); as well as herbicide compositions containing(A2) glyphosate (salt) and related compounds and herbicide from group (B); wherein components (A) and (B) are used in synergically effective ration.

EFFECT: effective controlling of weeds in grain crops.

6 cl, 70 tbl, 3 ex

FIELD: organic chemistry, herbicides, agriculture.

SUBSTANCE: invention describes a synergistic composition of herbicides comprising components (A) and (B) wherein (A) represents herbicide taken among the group of the formula (I):

wherein R1 means (C1-C4)-alkyl; R2 means (C1-C4)-alkyl; R3 means hydrogen atom; X and Y mean (C1-C4)-alkoxy-group; (B) represents one or two herbicides taken among the group of compounds or their acceptable forms: alachlor, metolachlor, acetochlor, dimetenamide, atrazine, cyanasin, metribusin, fluthiamide, nicosulfuron, rimsulfuron, primisulfuron, pendimetalin, sulcotrion, dicamba, mesotrion, isoxachlortol, metosulam, anilofos, fenoxaprop-ethyl, setoxydim, diclofop-methyl, MCPA, bromoxynil, pyridat, clopyralid, iodosulfuron-methyl, ethoxysulfuron, amidosulfuron, gluphosinat-amminium, isopropylammonium-glyphosate, imasetapir wherein components (A) and (B) are taken in the effective doses. Also, invention describes a method for control of weeds by using above indicated herbicide composition. Invention provides the development of the synergistic herbicide composition eliciting high activity.

EFFECT: improved method for control, valuable properties of composition.

6 cl, 26 tbl, 3 ex

The invention relates to the field of agricultural chemicals, in particular the compositions of agricultural chemicals in a form suitable for use with standard spraying equipment

The invention relates to a means to control weeds, in particular to the herbicide agent containing as an active ingredient (A) nitrile 1-(3-chloro-4,5,6,7-tetrahydropyrazolo-[1,5-a]-pyridine-2-yl)-5-(methylpropylamine)-4-pyrazolecarboxylate acid, and optionally, as an active ingredient (B) a substance selected from the group comprising bentazon, molinet, Diuron, thiobencarb, butachlor, pretilachlor, timepart, fenoxaprop-ethyl, clomipram, cinmetacin, bromobutyl, chinkara, mefenacet, pyrazosulfuron-ethyl, asbroker, chinaculture, tanishlar, cumyluron, MK 243, nitroanilide, anilofos, belforest, bifenox, SN-900, MSRA, nitrofen, oxadiazon, pendimethalin, simetryn, sulcotrione (SA), trifluralin, piperophos, perimutter, ethoxysulfuron, benzylbromide, pyrazolate, paradoxien, benzefoam, cycloaliphatic, cyhalofop, NBA-061 and azimsulfuron

The invention relates to herbicide compositions containing herbicide derived sulfonylureas and at least one serviced, choose from a wide range of serviceof

The invention relates to a method of controlling undesirable growth of plants, using a joint application, in the future, co-deposition, dimethenamid and at least one herbicide, herbicide compositions, including dimethenamid and at least one herbicide, and the use of such compositions for the destruction of unwanted vegetation growth

FIELD: anatomic embalming facilities.

SUBSTANCE: solution consists acetone as principal component producing dehydration effect on tissues and, in order to enhance defatting property of solution, the latter further contains hexane possessing strong defatting capacity, all components of solution being taken in specified proportions.

EFFECT: increased wear resistance of polymeric anatomic preparations and prolonged storage time thereof.

FIELD: anatomic embalming facilities.

SUBSTANCE: solution consists acetone as principal component producing dehydration effect on tissues and, in order to enhance defatting property of solution, the latter further contains hexane possessing strong defatting capacity, all components of solution being taken in specified proportions.

EFFECT: increased wear resistance of polymeric anatomic preparations and prolonged storage time thereof.

FIELD: medicine.

SUBSTANCE: method involves exposing blood to be preserved, to uniform magnetic field of 17000±100 nTesla units intensity and 2.5-3 Hz pulsation frequency during at least 2 h and then cooling it.

EFFECT: prolonged storage period; high erythrocyte resistance and osmoresistance.

FIELD: agriculture.

SUBSTANCE: the suggested herbicidal composition based upon a two-component mixture contains ethers based upon alcohols C8 of normal and isostructure or those of fraction C7-C9 2-methyl-4-chlorophenoxyacetic acid (I) and analogous ethers 3.6-dichloropyridine-2-carbonic acid (II) at weight ratio being (I):(II) being equal to (84-90):(10-16). Composition, also, contains an emulsifying agent -oxyethylated alkylphenols or higher fatty alcohols, a solvent - aromatic hydrocarbons or alcohols C7-C9, at the following ratio of components, weight%: ethers 2-methyl-4-chlorophenoxyacetic acid (I) 27-45, ethers 3.6-dichloropyridine-2-carbonic acid (II) 3-7, emulsifying agent 15-20, solvent - up to 100. The obtained herbicidal composition is of higher herbicidal properties along with decreased herbicidal loading upon environment.

EFFECT: higher efficiency of application.

12 ex, 2 tbl

FIELD: agriculture, in particular method for controlling of weeds.

SUBSTANCE: claimed method for controlling of weeds in tolerant maize cultures includes application of composition comprising (A) road spectrum herbicide from group (A1) glufosinate (salt) and analogs, (A2) glyfosate (salt) and analogs; (B) one or more herbicides from group (B1) cyanazin, alachlor, nicosulfurone, rimsulfurone, sulkotrion, mesotrion, and penthoxamid; (B2) pendimethalyne, methosulam, isoxaflutol, metribuzin, chloransulam, flumetsulam, linuron, florasulam, and isoxachlorotol; and (B3) bromoxinyl, chlorpyralid, tifensulfuron, MCPA (2-methyl-4-chlorophenoxyacetic acid), halosulfuron, and sulfosulfuron, wherein (A) and (B) components are in synergic effective ration. Also disclosed are herbicidal compositions including compound from group (A1) or )F2) and herbicide from group (B).

EFFECT: effective controlling of weeds in tolerant maize cultures.

6 cl, 55 tbl, 3 ex

FIELD: organic chemistry, agriculture, herbicide composition.

SUBSTANCE: invention relates to herbicide composition, containing conventional inert additives and mixture of a) herbicidically effective amount of substance satisfying the formula I [in formula R1 and R3 are the same or different C1-C4-alkyl; R4 and R5 together form groups of formulae: -C-R6(R7)-O-C-R8(R9)-C-R10(R11)-C-R12(R13)-(Z1), -C-R14(R15)-C-R16(R17)-O-C-R18(R19)-C-R20(R21)-(Z2), or -C-R22(R23)-C-R24(R25)-C-R26(R27)-O-C-R28(R29)-(Z3), wherein each R6-R29 is hydrogen; G is hydrogen or -C(X2)-X3-R31; X2 and X3 independently are oxygen; R31 is C1-C10-alkyl]; b) herbicidic synergic amount of at least one herbicide selected from group containing sulfonylureas, phenoxyacetic acids, as well as florsulam, tralcoxidim, klodinafol-propargil, phenoxaprop-P-ethyl, trifluramine, pendimethaline, picolinafen, etc. Composition also may contain safety effective amount of protective agent, such as chloquintocet-mexyl and additive (e.g., mineral oil or C8-C22-fat acid alkyl esters) in amount of 0-2 mass %. Also disclosed is method for selective controlling of weeds and grassy plants in cultural plants by treatment of cultural plants, seeds or seedlings thereof, or vegetation area thereof with claimed composition.

EFFECT: effective composition and method for weed controlling.

5 cl, 11 tbl, 7 ex

FIELD: organic chemistry, agriculture, herbicide composition.

SUBSTANCE: invention relates to herbicide composition, containing conventional inert additives and mixture of a) herbicidically effective amount of substance satisfying the formula I [in formula R1 and R3 are the same or different C1-C4-alkyl; R4 and R5 together form groups of formulae: -C-R6(R7)-O-C-R8(R9)-C-R10(R11)-C-R12(R13)-(Z1), -C-R14(R15)-C-R16(R17)-O-C-R18(R19)-C-R20(R21)-(Z2), or -C-R22(R23)-C-R24(R25)-C-R26(R27)-O-C-R28(R29)-(Z3), wherein each R6-R29 is hydrogen; G is hydrogen or -C(X2)-X3-R31; X2 and X3 independently are oxygen; R31 is C1-C10-alkyl]; b) herbicidic synergic amount of at least one herbicide selected from group containing sulfonylureas, phenoxyacetic acids, as well as florsulam, tralcoxidim, klodinafol-propargil, phenoxaprop-P-ethyl, trifluramine, pendimethaline, picolinafen, etc. Composition also may contain safety effective amount of protective agent, such as chloquintocet-mexyl and additive (e.g., mineral oil or C8-C22-fat acid alkyl esters) in amount of 0-2 mass %. Also disclosed is method for selective controlling of weeds and grassy plants in cultural plants by treatment of cultural plants, seeds or seedlings thereof, or vegetation area thereof with claimed composition.

EFFECT: effective composition and method for weed controlling.

5 cl, 11 tbl, 7 ex

FIELD: agriculture, in particular herbicide compositions.

SUBSTANCE: invention relates to weed controlling method for tolerant grain crops using (A) road spectrum herbicides selected from group (A1) glufosinate (salt) and related compounds; (A2) glyphosate (salt) and related compounds; and (B) one or more herbicides selected from group containing (B1) herbicides selectively effecting in grain crops especially against monocotyledonous weeds effecting on folia and/or soil (residual benefit); or (B2) herbicides selectively effecting in grain crops against monocotyledonous or dicot weeds effecting especially on folia; or (B3) herbicides selectively effecting in grain crops against monocotyledonous or dicot weeds effecting on folia or soil; or (B4) herbicides selectively effecting in grain crops against monocotyledonous or dicot weeds effecting on folia; wherein components (A) and (B) are used in synergic ration. Also are described herbicide compositions containing (A1) ) glufosinate (salt) and related compounds and herbicide from group (B); as well as herbicide compositions containing(A2) glyphosate (salt) and related compounds and herbicide from group (B); wherein components (A) and (B) are used in synergically effective ration.

EFFECT: effective controlling of weeds in grain crops.

6 cl, 70 tbl, 3 ex

FIELD: agriculture, in particular herbicide compositions.

SUBSTANCE: invention relates to weed controlling method for tolerant grain crops using (A) road spectrum herbicides selected from group (A1) glufosinate (salt) and related compounds; (A2) glyphosate (salt) and related compounds; and (B) one or more herbicides selected from group containing (B1) herbicides selectively effecting in grain crops especially against monocotyledonous weeds effecting on folia and/or soil (residual benefit); or (B2) herbicides selectively effecting in grain crops against monocotyledonous or dicot weeds effecting especially on folia; or (B3) herbicides selectively effecting in grain crops against monocotyledonous or dicot weeds effecting on folia or soil; or (B4) herbicides selectively effecting in grain crops against monocotyledonous or dicot weeds effecting on folia; wherein components (A) and (B) are used in synergic ration. Also are described herbicide compositions containing (A1) ) glufosinate (salt) and related compounds and herbicide from group (B); as well as herbicide compositions containing(A2) glyphosate (salt) and related compounds and herbicide from group (B); wherein components (A) and (B) are used in synergically effective ration.

EFFECT: effective controlling of weeds in grain crops.

6 cl, 70 tbl, 3 ex

FIELD: plant protection.

SUBSTANCE: invention provides herbicidal formulation based on 2,4- dichlorophenoxyacetic acid and 3,6-dichloropyridine-2-carboxylic acid at their weight ratio (50-90):(10-50)m respectively (taking in account their free-acid forms).

EFFECT: increased herbicidal activity and reliability of working characteristics.

2 cl, 3 tbl, 14 ex

FIELD: medicine.

SUBSTANCE: method involves using product containing spermatozoa treated by drying with freezing to humidity level of 1% and having injured membrane or spermatozoon head so that spermatozoon nucleus retains its genetic validity enough for fertilization. The spermatozoon heads fertilize an isolated oocyte after rehydration and microinjection being done. The retained genetic integrity is enough for fertilizing an oocyte and producing living descendants. Method involves collecting living mature spermatozoa, making spermatozoa suspension in special purpose physiological medium, freezing the spermatozoa suspension for producing frozen spermatozoa, drying the frozen spermatozoa or spermatozoa heads in vacuum to humidity level of 1%, making rehydration of spermatozoa or spermatozoa heads with injured membrane and selecting those retaining nucleus of genetic integrity. The selected spermatozoa or spermatozoa heads are used for fertilizing isolated oocytes with living descendants being produced.

EFFECT: enhanced effectiveness in producing living descendants.

39 cl, 5 dwg, 1 tbl

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