Method of treating aqueous medium to remove oil pollution and biological preparation for treating water medium against oil pollution

FIELD: oil pollution removal.

SUBSTANCE: biological preparation comprises substance carrier, microorganism growth factor, and biomass of microorganisms serving as oil destructors. Carrier is a composition made from Ca alginate gel, C14-C16-n-alkanes, and microorganism growth factor substance. Composition of biological preparation assists localization of microorganism growth density directly in the carrier near to interface between oil product and medium to be treated. Treatment method includes placing indicated dispersed biopreparation with microorganisms on water medium surface.

EFFECT: enabled use of well-workable granules and increased degree of purification.

5 cl, 2 tbl

 

The invention relates to the field of water purification from oil products and can be used to maintain natural ecosystems of water bodies and for treatment of domestic wastewater in different natural-climatic zones.

A known method of purification of water environment from pollution by oil products, including pre-cultivation of the oil destructive microorganisms and the subsequent introduction of culture in the treated water together with an activating reagent (SU, No. 1428809, 02 F 3/34,1985).

This method because of the technological gap between microorganisms and the reagent are not effective enough in case of emergency spills, not allowing you to quickly dispose of the oil film and leads to high levels of bacteria in the treated water.

Also known biologic for treatment of water environment from pollution by oil products, including increasing the biomass of the oil destructive microorganisms grown in a nutrient medium with subsequent drying (SU # 1076446, 02 F 3/34, 1982). However, such biological product does not provide the required for effective removal of the oil film increases the density of bacteria and their localization on the surface of the water environment.

More effective and the most similar analogues to the claimed technical solution is a biological preparation to clean the key of the aquatic environment from pollution by oil products, containing the substance with the carrier substance is a growth factor of microorganisms and microbial biomass-oil degrading, immobilized by recordatori biomass microorganism-oil degrading in the amount of mass media with the physical connection with their substance carrier, and a method of purification of water environment from pollution by oil products, including placing on the surface of the water environment of dispersed biological product with microorganisms-oil degrading, physically immobilized in with a positive buoyancy of the native in combination with a substance - factor in the growth of microorganisms, the activation of the oil destructive microorganisms through their interaction with the surface water environment of petroleum products in the presence of substance - factor in the growth of microorganisms, the sorption of oil carriers, biodegradation sorbed oil from disposal of hydrocarbons and removal of a biological product from the surface of the water environment (RU # 2081854, 02 F 3/34,1997).

Said means providing an efficient degradation of the oil film due to the localization of the oil destructive microorganisms on the surface of the water environment, does not exclude the interaction of a biological product with native microflora and thereby complicates its subsequent destruction.

Task, n is the solution which is aimed by the invention, is the creation of cost-effective and efficient means of cleaning the water environment from pollution by oil products.

This technical result, which can be obtained by implementing a technical solution of a biological product for cleaning the water environment from pollution by oil products, is to create a high-tech granular biological product with properties that provide localization process of increasing the density of microorganisms on the substrate in the area adjacent to the phase boundary of the oil-water interface, thereby ensuring the implementation of the above method of cleaning a water environment.

This technical result is achieved by biological purification of the water environment from pollution by oil products containing the substance with the carrier substance is a growth factor of microorganisms and microbial biomass-oil degrading, immobilized by recordatori biomass of microorganisms in oil degrading the material mass media with their physical connection with the substance of the medium due to the fact that, as a matter of media applied a composition of CA-alginate gel, n-alkanes with C14-C16and substance - growth factor, while the physical connection of the oil destructive microorganisms with the substance of the media carried out by what redstem the formation of droplets of a mixture of suspended biomass with ingredients of the composition of matter of the medium and the polymerization of the droplets with the formation of granules in aqueous solution, containing CA ions.

And also due to the fact that as an aqueous solution containing ions of CA applied an aqueous solution of CaCl2.

And also due to the fact that, as a matter of factor growth of microorganisms applied corn extract.

And also due to the fact that the drops are formed by the effect of the air jet on the jet of a mixture of suspended biomass and ingredients of the composition of matter of the media.

The technical result that can be obtained by carrying out the claimed method of purification of water environment from pollution by oil products is to reduce the duration of the purification of the water environment and increase the level of cleaning due to the localization of the process of increasing the density of microorganisms on the substrate in the area adjacent to the phase boundary of the oil-water interface while minimizing the interaction of microorganisms with native microflora.

This technical result is achieved by a method of purification of water environment from pollution by oil products, including placing on the surface of the water environment of dispersed biological product with microorganisms-oil degrading, physically immobilized in with a positive buoyancy of the native in combination with a substance-factor in the growth of microorganisms, the activation of microorganisms-the nave is of destruction through their interaction with the surface water environment of petroleum products in the presence of substance-factor in the growth of microorganisms, sorption of oil carriers, biodegradation of sorbed oil from disposal of hydrocarbons and removal of a biological product from the surface of the water environment due to the fact that as dispersed biological product with microorganisms-oil degrading use of the biological product that includes a carrier substance, the substance is a growth factor of microorganisms and microbial biomass-oil degrading, immobilized by the distribution of biomass of microorganisms in oil degrading the material mass media with their physical connection with the substance of the medium due to the fact that, as a matter of media applied a composition of CA-alginate gel, n-alkanes with C14-C16and substance - factor in the growth of microorganisms, while the physical connection of the oil destructive microorganisms with the substance of the medium introduced through the formation of droplets of a mixture of suspended biomass with ingredients of the composition of matter of the medium and the polymerization of the droplets with the formation of granules in an aqueous solution containing ions of CA.

The essence of the proposed technical solution is illustrated in the following example the manufacture of a biological product and the implementation of the method.

For increasing the biomass of the oil destructive microorganisms in a nutrient medium in a container, for example with a volume of 750 ml, make 100 ml of p the test environment, consisting of mycopathologia broth (N. Rozanov, “Handbook of microbiological techniques. - M.: Selhozizdat, 1957, page 284), 2 g of ammonium acetate and microorganisms, for example (PMBC, Acinetobacter sp.B 5064-VSB-567 or Acinetobacter calcoaceticus In 5961-JN-2). In the thus created a nutrient medium establish a pH of 6.5, a container with a nutrient medium and microorganisms-oil degrading mounted on the rocking chair in thermostat and providing oscillation frequency 200-230 1/min and a temperature of 30°within two days bring the density of microorganisms in a nutrient medium, for example, up to 109cells/ml (20 g/l dry biomass). The suspension of the biomass of microorganisms-oil degrading carried out by intensive mixing.

For immobilization of microorganisms by oil degrading recordatori suspended biomass microorganism-oil degrading in the amount of mass media with the physical connection with their substance carrier prepared 1% aqueous mixture of sodium alginate (e.g., in the amount of 100 ml for the mentioned number of suspensions biomass), carefully mixed and incubated for 2-3 hours for complete absorption of water by fragments of sodium alginate. To the thus obtained mixture with sodium alginate add substances - growth factors, such as corn extract (N. Rozanov. Handbook of microbiological technique M: Selhozizdat, 1957, 284 pages) in an amount of 10 g per 100 ml of the mixture, and n-alkanes from C14-C16in the liquid state, such as hexadecane in an amount of 10 g per 100 ml of the mixture.

The mixture of sodium alginate and suspended biomass in equal volumes are mixed by a homogenizer for 2-3 minutes, then via a pump (e.g., peristaltic at 50 ml/h) is injected through a nozzle into a zone of airflow supplied to the region of the ejection, for example for the formation of granules from 0.18 to 0.55 mm with a speed of 0.002 m3/min. Formed by the effect of the air jet at the jet of the mixture drops fall into an aqueous solution containing ions of CA (for example, 2% aqueous solution l2), where due to the chemical compound of calcium ions with sodium alginate and polymerization in the process of aging (for example, for 30 min) with the formation of drops of CA-alginate gel they acquire the properties of a separate body and become granules. When this occurs the physical connection of the oil destructive microorganisms with the material of the granules, and thus obtained granules are carriers recordatorios suspended biomass immobilized microorganisms-oil degrading and where the substance of the media applied the composition of Issa-alginate gel, n-alkanes from C14-C16and substance is a growth factor. The granules were filtered, washed and placed in a physiological solution.

The presence of granules of n-alkanes from C14-C16first , it reduces the density of the substance, making granules carriers having positive buoyancy, and, secondly, ensures the diffusion of microorganisms due to their complete assimilation by microorganisms. The presence of granules of a substance is a growth factor of microorganisms provides an increase in the density of microorganisms directly in the media, and in combination with positive buoyancy localization of this process in the area adjacent to the phase boundary of the oil-water interface.

Biopreparation is resuspendable in water media-granules with microorganisms-oil degrading, physically immobilized in having positive buoyancy media made from a material that provides a diffusion of microorganisms, and containing microorganisms-oil degrading in combination with a substance - factor in the growth of microorganisms. Pellets range in color from light gray to beige, a range from +15° to +30°With, the titer of living cells -1·107cells/ml and the storage temperature range from +15° to +30°C.

For cleaning the water environment from oil pollution of nepobedimosti water environment place (put) dispersed (resuspending based 150 l per 1 ha) biological product. Due to the positive buoyancy of the biological product is concentrated in the surface area of greatest contamination. Due to the physico-chemical properties of substances media are sorption carriers of petroleum products from the surface of the water environment, and due to the activation of microorganisms interaction with the surface water environment of petroleum products in the presence of a substance - growth factor microorganisms produce biodegradation of sorbed oil from disposal of hydrocarbons. Thus, the diffusion concentration gradient, an increase in the density of microorganisms in the media and their access to the free state are a constant source of the oil destructive microorganisms in the area adjacent to the phase boundary of the oil-water interface. Retains its state of aggregation of the biological product is removed from the surface of the water environment along with recycled material, and if necessary bring it to the full decomposition due to the assimilation of substances of microorganisms and media under the influence of the indigenous microflora.

The results of biodegradation (destruction) of oil-free cells immobilized cells and immobilized cells with a growth regulator following:

The number entered immobilized cells

(Acinetobacter sp.B 5064-VSB-567) 0.1 ml (0.05 ml suspension of CL is current)

The volume of water in samples of 50 ml

The surface area of each sample is 91 cm2.

No. sampleThe time of the experimentThe current concentration of oil products in the sample
dayimmobilized cells without substance is a growth factor, mgimmobilized cells with a substance - growth factor (corn extract), mg
 tSiS2
10660580
25605530
310455420
415290240
520175110
6258575
73049<10

The results of biodegradation (destruction) of oil-free cells:

The number included the suspension of free cells, 1 ml

The volume of water in samples of 100 ml

No. sampleThe time of the experiment dayTech is based on the concentration of oil products in the sample of free cells, mg
 tS3
10570
25540
310490
415360
520340
625-
730200

1. The biological purification of the water environment from pollution by oil products containing the substance with the carrier substance is a growth factor of microorganisms and microbial biomass-oil degrading, immobilized by recordatori biomass microorganism-oil degrading in the amount of mass media with the physical connection with their substance carrier, characterized in that, as a matter of media applied a composition of CA-alginate gel, n-alkanes with C14-C16and substance-factor in the growth of microorganisms, while the physical connection of the oil destructive microorganisms with the substance of the medium introduced through the formation of droplets of a mixture of suspended biomass with ingredients of the composition of matter of the medium and the polymerization of the droplets with the formation of granules in an aqueous solution containing ions of CA.

2. Biological preparation according to claim 2, otlichuy is the, an aqueous solution containing ions of CA applied an aqueous solution of CaCl2.

3. Biological preparation according to claim 3, characterized in that the substance is a growth factor microorganisms applied corn extract.

4. Biological preparation according to one of claim 2 and 3, characterized in that the drops are formed by the effect of the air jet on the jet of a mixture of suspended biomass and ingredients of the composition of matter of the media.

5. The method of purification of water environment from pollution by oil products, including placing on the surface of the water environment of dispersed biological product with microorganisms-oil degrading, physically immobilized in with a positive buoyancy of the native in combination with a substance - factor in the growth of microorganisms, the activation of the oil destructive microorganisms through their interaction with the surface water environment of petroleum products in the presence of substance - factor in the growth of microorganisms, the sorption of oil carriers, biodegradation of sorbed oil from disposal of hydrocarbons and removal of a biological product from the surface of the aqueous medium, characterized in that as dispersed biological product with microorganisms-oil degrading use of the biological product according to claim 1, and the activation of the oil destructive microorganisms carry out the in the matter of the media support the growth of the density of microorganisms on the substrate and diffusion of microorganisms with access to the free state.



 

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