Method for production of pancreatic protein hydrolyzate and broth for bifidus bacteria cultivation using the same

FIELD: biotechnology, microbiology, broth for bifidus bacteria cultivation.

SUBSTANCE: claimed broth for bifidus bacteria cultivation contains as main component pancreatic protein hydrolyzates of casein or forcemeat containing 700-900 mg% of amine nitrogen. Hydrolyzable mixture additionally contains 20-40 g/l of cow or pork liver waste. Broth also contains sodium chloride 4.0-5.0 g; L-cystine hydrochloride 0.10-0.15; microbiological agar 0.75-1.00 g; yeast autolyzate 300.0-400.0 ml; pancreatic casein hydrolyzate containing 700-900 mg% of amine nitrogen, diluted with distilled water up to amine nitrogen content of 140-160 mg%, 300.0-350.0 ml; pancreatic forcemeat hydrolyzate containing 700-900 mg% of amine nitrogen, diluted with distilled water up to amine nitrogen content of 140-160 mg%, 300.0-350.0 ml; 40 % lactose solution 25.0 ml.

EFFECT: new nutrient medium completely satisfying to bacterium population requirements.

2 cl, 3 tbl, 3 ex

 

The invention relates to biotechnology, microbiological industry, manufacturing of culture media for cultivation of bacteria.

Modern principles of therapeutic correction of dysbiotic changes and restore used is based primarily on proposed almost a century ago I. I. Mechnikov method enteral introduction of live cultures of beneficial bacteria. Currently, the widespread construction of preparations of probiotics containing as the active principle of the living representatives of normal microflora of the person.

The most important tasks aimed at improving the population, are studies in the development of new effective drugs-probiotics, improvement manufactured forms, intensification and increase of economic efficiency of production and guaranteed for quality products. The increasing demand for drugs-probiotics and nutritional supplements leads to the need for seeking new ways to create affordable means of obtaining biomass of acid-forming bacteria [1].

Phylogenetically determined that bifidobacteria, which are obligate anaerobes, in natural conditions, mainly live in the conditions of the large intestine, in which the protein component of apatania significantly hydrolyzed by digestive enzymes of the microorganism. Under cultivation of bifidobacteria main substrates of nutrient media, limiting growth and affect the physiological activity of bacterial populations are sources of nitrogen and carbon. Demand data of the bacteria to the power sources, many of which for the most active growth need to be ready for all amino acids, necessitates the development of methods for obtaining basics of nutrient media that meets all the needs of micro-organisms for nutrients and providing a high concentration of viable bacteria in the biomass. In addition, the study needs of bifidobacteria showed that for optimal growth they require additional growth factors (vitamins, purine and pyrimidine bases, amino sugar and others), which should be submitted in finished form, as bifidobacteria are unable to synthesize all components included in the composition of cell substances [2, 3].

Therefore, the currently used culture medium for the accumulation of biomass of bifidobacteria are characterized by the diversity of its composition. In the production of culture media used substances and substances that are completely different in nature. Cultivation of bifidobacteria is carried out in environments, which are based hydrolysates and extrac the s of plant and animal origin.

Considering the fact that the main purpose of the production of culture media is producing Biopharmaceuticals, they imposed a number of additional requirements: the environment must be of high quality, cost-effective and relatively simple to manufacture [4].

For cultivation of bacteria is the most common environment Blaurock [2], which contains, g/l:

Peptone - 10,0

D(-) - lactose - 10,0

Sodium chloride - 5,0

L-cystine hydrochloric acid - a 0.1

The microbiological agar - 0,75

Liver broth to 1 l

pH 6,8-7,0

Hepatic cysteine media for cultivation of bacteria is considered to be optimal, however, this environment contains expensive components and biological materials (peptone enzyme, L-cystine hydrochloric acid, beef liver), so to obtain a biomass in an industrial environment appears to be economically feasible.

Hydrolysate - milk (GM) medium (5) has the following composition, g/l:

Peptone - 2,0

Sodium chloride - 2,0-3,0

L-cystine hydrochloric acid and 0.1 - 0.15

D(-) - lactose - 10,0

The microbiological agar - 2,5

Hydrolyzed milk products milk - up to 1 l

pH 7,2-7,4

GM medium contains as a nutritional basis only gidrolizovannogo milk with the final concentration of amino nitrogen 70-110 mg % and not rich enough in what radiantly, stimulating the growth of bifidobacteria. Gidrolizovannogo milk can not fully meet the needs in the sources of nitrogen nutrition, vitamins and growth factors, as milk is not a natural habitat of bifidobacteria. Given that bifidobacteria do not have casualities activity, in the preparation of the environment of the use of the base in the form of hydrolyzed milk obtained with the use of expensive biological raw materials and components (cow milk, nonfat, Pancreatin enzyme).

Known nutrient medium for cultivation of bifidobacteria “Baclofe” (6), including the composition, g/l:

D(-) - lactose - 10,0

Sodium chloride - 5,0

The microbiological agar - 0,75

Enzymatic hydrolysate of muscle tissue of the fruit of cows 6-8 months of age, and/or pigs 2.5 to 3 months of age, and/or sheep last period of pregnancy with the final concentration of amino nitrogen 100-140 mg % - up to 1 l

pH 7,1±0,1

The main advantage of protection “Baclofe” is an exception to the composition of the medium expensive component L-cysteine hydrochloric acid, however, the use of biological raw materials in aborted fetuses of cows, pigs and sheep appears to be unsafe from the epidemiological point of view, and the slaughter of animals for such pregnancy of economic and uneconomic.

Nutrient media for cultivation of bacteria on the basis of enzymatic hydrolysate molozivnyi casein-whey mass [7] contains g/l:

Iron sulfate - 0,002-a 0.1

Sodium chloride 4,0-6,0

Glucose 8,0-12,0

The microbiological agar - 0,75-1,0

Ascorbic acid - 0,8-1,0

The enzymatic hydrolysate molozivnyi casein-whey

mass - to 1 l

Under cultivation of bacteria in a nutrient medium not provided with sufficient growth rate, since the content of amino nitrogen in the medium (70-110 mg %) is not sufficient to produce biomass with a high content of viable cells. Given that colostrum is expensive seasonal raw materials, the environment trudnovospituemyh in an industrial environment.

Known nutrient media for cultivation of bacteria on the basis of the acid-alkaline hydrolysate legumes (8), g/l:

The acid-alkaline hydrolysate - 120-150 mg %

Peptone - 8,0-15,0

D(-) - lactose 3,0-12,0

Agar microbiological 0,7-0,9

Sodium chloride 6,0-8,0

Water up to 1 l

pH of 7.0, and 7.1

The acid-alkaline hydrolysate of vegetable raw materials (pea, lupine, alfalfa) may not provide the full needs of bifidobacteria in all the required power sources, which affects the growth properties of a medium (according to the data of literature, for environments using hydrolysates of vegetable raw materials give good growth fungal culture [4]). Because of an inadequate amount of free amino acids decreases the growth rate of microorganisms, which affects the number of viable bacteria in the biomass. Culture morphologically heterogeneous, in test tubes marked fuzzy picture of growth, these symptoms can lead to loss of valuable production properties.

The closest analogue to the claimed medium is casein-yeast medium - KD-5 [9], containing as the basis pancreatic hydrolysate of casein and yeast autolysate:

Sodium chloride - 5.0 g/l

Lactose - 10.0 g/l

L - cystine hydrochloric acid 0.1 g/l

The microbiological agar - 0.75 g/l

Yeast autolysate - 650 ml/l

Pancreatic hydrolysate of casein with the final concentration of amino nitrogen (150±10) mg % - 350 ml/l

The basis of casein - yeast environment consists of semi-finished products prepared according to known technologies:

1) pancreatic hydrolysate of casein is produced by digestion of a colloidal suspension of casein enzymes minced pancreas at pH of 8.2 to 8.3 and a temperature of 37°C for 10 days before reaching a final concentration of amino nitrogen 500-600 mg % (10);

2) yeast autolysate is obtained by autolysis of pressed Baker is different yeast at a temperature 53-56° C for 2-3 days. Then diluted with water ratio of 3 l per 1 kg of yeast and sterilized at a temperature of 120°C for 30 minutes the Final concentration of amino nitrogen yeast autolysate 200-250 mg % [4];

Casein-yeast medium is widely used in the production of Bifidumbacterin dry. Dvukhkomponentnoi sources of nitrogen nutrition determines the growth properties of the medium and provides obtaining biomass of bifidobacteria at a concentration of 107-108CFU/ml, followed by a reliable preservation of viable microbial cells in the process of freeze drying.

However, besides the positive qualities of casein-yeast medium is not without drawbacks. The culture medium can also be difficult to manufacture due to the time of preparation of pancreatic hydrolysate of casein and yeast autolysate, requires a large consumption of biological raw materials (yeast, pancreas) and expensive components (casein, L - cysteine hydrochloric acid).

Due to the high demands of bifidobacteria to sources of nitrogen, carbon, vitamins and mineral nutrition, providing fully the needs of micro-organisms and affect the physiological activity of bacterial populations, biomass yield in casein-yeast medium does not reach the maximum of the values. In addition, casein-yeast medium is used only in the final stages of obtaining biomass of bifidobacteria and is used as the base substrate environment of the suspension. Getting uterine culture of bifidobacteria with all previous transfers carried out in expensive hepatic cysteine environment (environment Blaurock).

When sowing uterine culture obtained in the environment of Blaurock, casein-yeast medium is observed lengthening of the lag-phase and the decrease in specific growth rate (μ) in exponential phase (definition of spend on a growth curve of a growing culture). These parameters indicates the non-optimal conditions for the growth of biomass due to the fact that microorganisms are stressed when reseeding in exponential growth phase in a different environment and they need time for physiological adaptation. In addition, subcultures culture environment Blaurock in casein-yeast environment increase the risk of changing production properties and contribute to the emergence of spontaneous mutants that affect the homogeneity of the bacterial population.

The task of the invention is to develop a nutrient medium for growing bacteria with high growth stimulating activity and providing the concentration of viable cells n is less than 10 9CFU/ml at all stages of biomass of bifidobacteria.

The technical result consists in obtaining new cost-effective nutrient medium for cultivation of bifidobacteria, which includes pancreatic hydrolysates of casein and meat-pressed, obtained by a modified method and best meets the needs of the bacterial population.

The invention consists in that a nutrient medium for cultivation of bifidobacteria contains in its composition as the basis pancreatic hydrolysates of casein and meat-spin with the content of amino nitrogen 700-900 mg %, obtained by a modified method. Nutrient medium for cultivation of bifidobacteria contains:

Sodium chloride - 4,0-5,0 g

L - cystine hydrochloric acid - 0,10-0,15 g

The microbiological agar - 0,75-1,00 g

Yeast autolysate is 300-400 ml

Pancreatic hydrolysate of casein with the content of amino nitrogen 700-900 mg %, diluted with purified water until the content of amino nitrogen 140-160 mg % - 300-350 ml

Pancreatic hydrolysate meat-spin with the content of amino nitrogen 700-900 mg %, diluted with water, treated to the content of amino nitrogen 140-160 mg % - 300-350 ml

Lactose 40%solution - 25 ml (included aseptically before cultivation)

Ready nutrients from the food should be sterile and contain amino nitrogen (150± 10) mg %, a pH of 7.3±0,2.

Unlike the prototype, the culture medium contains pancreatic hydrolysate meat-spin (first recyclable after cooking meat infusion) with the content of amino nitrogen 700-900 mg % and pancreatic hydrolysate of casein with the content of amino nitrogen 700-900 mg %, obtained by a modified method. In the medium changed the ratio of the components of the framework: the content of yeast autolysate with the concentration of amino nitrogen 200-250 mg % after optimization of technological parameters (reduction of the substrate, changing the duration of autolysis, the change in the temperature regime) is 30%, the increase in the concentration of amino nitrogen in hydrolysates of casein and meat-pressed to 900 mg % also allows to reduce the consumption of semi-finished products.

Aseptic introduction of lactose in the composition of the nutrient medium in the form of a 40% solution before cultivation allows you to browse with the best physical properties and high growth stimulating activity.

The set of distinctive features is allowed to obtain the following advantages.

Cooking environments of several types of raw materials helps to get in a nutrient medium the most optimal and balanced composition to ensure the complex needs of bifidobacteria, as it allows to avoid PE is asimenia environment among compounds in the disadvantage of the other. Introduction in the nutrient medium composition advanced pancreatic hydrolysate meat-spin contributes to a more complete satisfaction of microorganisms in sources of nitrogen nutrition and growth factors, as casein is a protein in milk that nature intended for performance of alimentary functions, but the biological value it is inferior to the proteins of meat. Proteins beef have better aminogram are determined and a higher biological value due to amino acid composition (cystine, alanine, aspartic and glutamic acids, serine, Proline, and others).

Introduction as an additional substrate proteolysis and stimulator of enzymatic reactions wastes liver beef allows you to optimize the processes of hydrolysis of casein and meat-spin when changing the enzyme-substrate ratio and conditions of the hydrolysis. Liver of cattle is a source of valuable proteins, glycogen, lecithin, iron, a large number of growth factors (nicotine, pipelinewall, Pantothenic, folic acid, Biotin and others), in this regard, in enzymatic hydrolysates add waste beef liver, containing major hepatic ducts and utilized above in the preparation of hepatic decoctions and extracts.

The careful observance of the conditions g is kolisa (temperature - 45°C, pH of 7.9 to 8.0) the process of enzymatic lysis by casein or meat-wringing completed within 24-48 h, which allows to reduce the duration of hydrolysis in 3-5 times. The use of the claimed methods of preparation of pancreatic hydrolysate at the optimum enzyme-substrate ratio allows to obtain a hydrolysate with a high degree and depth of the cleavage of the protein, which is primarily a mixture of amino acids (amino nitrogen 700-900 mg %) and low molecular weight peptides, and the use of waste products in the form of minced meat-spin and waste beef liver contributes to a significant reduction of production nutrient medium with the subsequent reception of the preparation of higher quality (table 1).

As a source of vitamins and an additional source of nitrogen nutrition use yeast autolysate. In contrast to the proteins of meat, bakery yeast protein content is slightly lower, but richer in carbohydrates, vitamins and minerals. The main source of carbon supply for the cultivation of bifidobacteria is the disaccharide lactose (milk sugar). In a nutrient medium containing carbohydrates and subjected to high temperatures, the processes of caramelization, humification, malonodinitrile, which leads to loss of the source to which icesta sugar and the appearance of dark-colored degradation products of carbohydrates, has inhibiting the growth of microorganisms action. Aseptic introduction of lactose in the composition of the nutrient medium in the form of a 40% solution before cultivation allows to obtain a nutrient medium with the best physical properties and high growth stimulating activity.

The study of the dynamics of the growth of bacterial populations allows the use of the inventive environment for the accumulation of biomass in obtaining uterine culture, which in turn helps you optimize cultivation of bifidobacteria. Sowing uterine culture obtained in the present environment, ensures the growth of bifidobacteria with minimum delay, and the course is limited to the growth solution allows lactose to increase biomass maximum yield reached to the stationary phase. The accumulation of biomass in a nutrient medium under conditions of periodic cultivation allows to obtain a biomass with a high physiological activity (not less than 109CFU/ml) (table. 2).

When morphological verification bifidobacteria grown in the proposed environment, comply with the requirements: smears are fixed gram forked sticks Y - or V-shape from 0.5 to 1.3×1.5 to 8 μm, which are arranged in pairs, sometimes the chains do not form spores and capsules.

Series bifidumbakterin dry, the floor is built by cultivation in the claimed nutrient medium, have good viability and antagonistic activity (table 3).

The invention is illustrated by the following examples.

Example 1. Preparation of pancreatic hydrolysate of casein. 6 kg of casein dissolved in 30 l of warmed up to 45°purified water, adjust pH to 8.8-9.0 in the introduction of the mixture of anhydrous sodium carbonate. With periodic mixing casein swells within 3-5 hours

In the reactor pour 70 l of warmed up to 45°With purified water and loaded through a hatch colloidal suspension of casein. In the hydrolyzable mixture establish a pH of 7.8 to 8.0, add 7 kg minced pancreas and 2-4 kg of waste liver of cattle or pigs, cleaned from fat and films and shredded meat grinder.

Hydrolysis of casein lasts up to 2 days at a constant temperature of 45°and 7,8-8,0 to achieve a final concentration of amino nitrogen 700-900 mg %. The resulting hydrolysate in the amount of 100 liters filtered through mickalene the canvas in the digester. Then in the hydrolysate set pH 4.5 to 4.7 by the addition of 2.5 n hydrochloric acid. After boiling, clarification and filtration administered chloroform (5 ml/l) and stored at a temperature of (5±3)°within 3 months.

Example 2. Preparation of pancreatic hydrolysate meat-spin

As syriopoulou stuffing-centrifuge, obtained after cooking meat water. In a reactor filled with 100 l of purified water, with constant stirring load through hatch 40,0 kg minced meat-spin. In the hydrolyzable mixture establish a pH of 7.8 to 8.0, add 7 kg minced pancreas and 2-4 kg of waste liver of cattle or pigs, cleaned from fat and films and shredded meat grinder.

Hydrolysis lasts up to 2 days at a constant temperature of 45°and 7,8-8,0 to achieve a final concentration of amino nitrogen 700-900 mg %. The resulting hydrolysate in the amount of 100 liters filtered through mickalene the canvas in the digester. Then in the hydrolysate set pH 4.5 to 4.7 by the addition of 2.5 n hydrochloric acid. After boiling, clarification and filtration administered chloroform (5 ml/l) and stored at a temperature of (5±3)°within 3 months.

Example 3. Nutrient media for cultivation of bacteria.

300 ml of pre-filtered through a cotton-gauze filter yeast autolysate combined with 350 ml of pancreatic hydrolysate of casein, diluted with purified water until the content of amino nitrogen 140-160 mg %, and 350 ml of hydrolysate meat-spin, diluted with purified water until the content of amino nitrogen 140-160 mg %. In a nutrient medium add 5 g of sodium chloride and mix thoroughly. the pH of the mixture down 20% R is the target sodium hydroxide to a value of (7,5± 0,1), then thoroughly mixed, heated to 100°, boil on low heat for 15 min and incubated at room temperature for 1 hour. After filtration through mickleby filter the resulting mixture was heated to 80°To contribute 0.75 g of agar microbiological and boil while stirring for 5 minutes to fully melt the agar. Add 100 mg of L - cysteine hydrochloric acid and mix thoroughly. Measure the volume received and, if necessary, bring purified water to (1000±10) ml.

Sterilized in a steam sterilizer at a temperature of (110±1)°and a pressure of 0.05 MPa for 30 minutes

Prepared nutrient medium should be sterile and contain amino nitrogen (150±10) mg %, a pH of 7.3±0,2.

Before use in a nutrient medium make the estimated amount of the 40% solution of lactose, previously subjected to fractional sterilization fluid vapor.

Literature

1. Naiyomah. Technology products preventive nutrition, M., 2001, s-182.

2. App. Microbiology of milk and milk products. M., 1999, s-176.

3. RF patent 2169763, With 12 N 1/20, publ. 27.06. 2001 Dairy nutrient medium to obtain a liquid concentrate of bifidobacteria.

4. Guide to Microbiology, clinical picture and epidemiology of infectious diseases edited by Prof. Nov-Verezhnikova, M., 1962,vol. 1, s-342, s.

5. GOST 25102-82 or SanPiN 42-123-49-40-88 paragraph 5.2 and 7.2.

6. RF patent 2002801, With 12 N 1/20, publ. 1993 Nutrient medium “Baclofe” for the cultivation of bifidobacteria.

7. RF patent 2080376, With 12 N 1/20, publ. 27.05.97, Nutrient media for cultivation of bacteria.

8. RF patent 94026139, With 12 N 1/20, publ. 10.05.96, Nutrient media for cultivation of bacteria.

9. Regulations for production No. 625 Bifidumbacterin dry from 16.12. 1996, p.150-151.

10. Vinciguerra. Technology culture media in the production of bactericidal drugs, Tbilisi, 1963, p.78-80.

11. RF patent 2027754, IPC C 12 N 1/20, publ. 27.01.95, Nutrient medium for cultivation of microorganisms and a method of obtaining a pancreatic hydrolysate of casein.

12. RF patent 2080376, IPC C 12 N 1/20, publ. 27.05.97, Nutrient media for cultivation of bacteria.

1. The method of obtaining pancreatic protein hydrolysate, comprising the hydrolysis of casein or meat-spin enzymes minced pancreas of cattle, followed by purification, characterized in that the hydrolyzable mixture impose additional waste liver beef or pork in the amount of 20-40 g/l and the hydrolysis is carried out to achieve the concentration of amino nitrogen 700-900 mg%.

2. Nutrient medium for cultivation of bifidobacteria containing pancreatic hydrolysate of casein, yeast autolysate, sodium chloride, agar microbiological, L-cystine hydrochloric acid, lactose, characterized in that it further comprises pancreatic hydrolysate meat-pressed in the following starting components:

Sodium chloride 4,0-5,0 g

L-cystine hydrochloric 0,10-0,15 g

Agar microbiological 0,75-1,00 g

Yeast autolysate 300,0-400,0 ml

Pancreatic hydrolysate of casein with the content of amino nitrogen 700-900 mg%,

diluted with purified water until the content of amino nitrogen 140-160 mg% 300,0-350,0 ml

Pancreatic hydrolysate meat-spin with the content of amino nitrogen 700-900 mg%, diluted with purified water until the content of amino nitrogen 140-160 mg%

300,0-350,0 ml

Lactose, 40%solution of 25.0 ml



 

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FIELD: microbiology, medicine.

SUBSTANCE: invention relates to method of diagnosis and treatment of intestinal yersinioses and pseudotuberculosis. Claimed method includes Yersinia enterocolitica and Yersinia pseudotuberculosis isolation from clinic and other material. Broth (I-agar) contains (g/l): peptone 19.0-21.0; yeast extract 1.9-2.1; mannitol 19.0-21.0; sodium pyruvate 1.9-2.1; sodium chloride 0.95-1.05; magnesium sulfate 0.009-0.011; sodium deoxycholeta 0.48-0.53; neutral red 0.028-0.032; crystal violet 0.009-0.0011; agar-agar 11.9-13.1; irgasane 0.0039-0.0041; and balance: distilled water.

EFFECT: simplified broth composition with improved selectivity to Y. pseudotuberculosis without essential alteration of other medium characteristics.

3 tbl, 1 ex

FIELD: biotechnology, microbiology, in particular broth production for cultivation of bifidobacteria and lactobacillus.

SUBSTANCE: claimed method includes water-based broth production. Water used for broth production is pretreated by non-filtered modulated ultraviolet light containing in radiant flux not less than 30 % of ultraviolet quantum with wave length less than 350 nm, for period sufficient to increase water intrinsic energy by not less than 2 times. Method also includes measurement of water intrinsic energy variation before treatment and during whole treatment process.

EFFECT: microorganisms with activated cultural properties; decreased cultivation time.

5 tbl, 2 ex

FIELD: foodstuff industry, biotechnology, agriculture.

SUBSTANCE: Propionibacterium acnes strain Ac-1450/26 is produced by multiple reseeding cells of starting Propionibacterium acnes Ac-1450 strain, deposited in All-Russian State collection of microorganism strains for veterinary and stockraising. Said strain represents a producer of foodstuff protein. Using of foodstuff protein producing strain makes it possible to avoid environment pollution when manufacturing of protein foodstuff, to increase protein specific yield, to simplify and accelerate manufacturing process, to simplify equipment and utilize waste of industries using natural raw materials.

EFFECT: improved and environmentally friendly method for producing of protein foodstuff.

2 tbl, 10 ex

FIELD: medicine microbiology, in particular technology for reproduction of brucellous bacteriophage.

SUBSTANCE: brucellous bacteriophage Bk2 is reproduced in liquid synthetic nutrient medium. Nutrient medium is used both to provide suspension of growth strain and further to reproduce of bacteriophage. Nutrient medium contains glucose as additional carbon source, amino acids and salts, and additionally 10-2-10-3 M of calcium sulfate. Method of present invention allows the ability to increase final brucellous bacteriophage Bk2 concentration wherein bacteriophage is reproduced by 48-72 h.

EFFECT: brucellous bacteriophage reproduction with increased bacteriophage concentration.

3 ex

FIELD: biotechnology, in particular probiotics for stock-raising.

SUBSTANCE: Strain Clostridium cellobioparum is isolated from caw punch dominant microflora and identified using conventional microbiological methods. Strain Clostridium cellobioparum ferments complex feedstuff polysaccharides, activates processes of digestion, volatile fat acid synthesis, whereby increases cow dairy productivity. Preparation obtained using strain of present invention contains 109-1010/g of viable cells.

EFFECT: feedstuff additive of improved characteristics.

3 ex

FIELD: biotechnology, microbiology.

SUBSTANCE: the strain of microorganism Serratia marcescens GISK N 268 - a producer of thermolabile enterotoxin is isolated from a patient with wound infection. Isolation of the new strain provides expanding assortment of strains as producers of thermolabile enterotoxin. The strain shows high productivity.

EFFECT: valuable properties of strain.

1 tbl, 2 ex

FIELD: biotechnology, microbiology, medicine.

SUBSTANCE: invention relates to the strain Lactobacillus paracasei CNCM I-2116 used for diarrhea prophylaxis causing by pathogenic microorganisms. Supernatant of this strain culture elicits ability to prevent colonization of intestine with pathogenic microorganisms causing diarrhea also and this strain is designated for preparing agent used for prophylaxis and/or treatment of disorders associated with diarrhea. Agent for oral administration represents therapeutically effective dose of the strain L. paracasei CNCM I-2116 or supernatant of its culture and acceptable foodstuff. Invention provides the enhanced viability of the strain in its applying and effectiveness in prophylaxis of adhesion to intestine cells and invasion to intestine cells of pathogenic microorganisms causing diarrhea.

EFFECT: valuable medicinal properties of strain.

5 cl, 8 dwg, 10 ex

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