Method for production of peloid water extract

FIELD: method for production of bioproducts from native divot peloid with high biological activity.

SUBSTANCE: claimed method includes extraction of fine dispersed divot peloid having plant residual decomposition ratio of 50 % or more with sodium hydrocarbonate-containing water at elevated temperature under intensive agitation to produce homogeneous suspension followed by separation under certain conditions.

EFFECT: ecologically pure concentrated peloid extract with high biological activity.

3 ex

 

The invention relates to a method for producing bio-based products, in particular aqueous extracts from native peat mud, and can be widely used in veterinary medicine, medicine, agriculture - crop production, horticulture, livestock, poultry.

A method of obtaining the extract from the sludge of mud, including the grinding of dirt, humidity 50-60% until pasty state, its extraction of 96%ethanol twice at a ratio of 6 to 10 volumes of alcohol per 1 kg of raw material at a temperature of 30-50° and With intensive stirring for 20-25 minutes, defending spirography weight for 3-4 hours, then poured alcohol micelle. In merged micelle add vaseline oil in quantities that provide extract the desired concentration, and the distillation of alcohol are under vacuum 05-07 ATM at a temperature of not higher than 50° With periodic stirring. The remaining extract assert (waste mostly salt water), and then dissolved in liquid paraffin (EN, p. No. 2073999 And 61 To 35/10).

The disadvantage of this method is incomplete extraction of nutrients due to oxidative processes occurring during extraction in alcohol, and subsequent distillation of the alcohol adding paraffin oil having poor absorption and poor extraction decrease in the percentage allocation to extract biologically active substances contributes to insufficient temperature 30-50° C.

A method of obtaining extracts with antifungal activity from lowland ozokeritovogo peat, including the freezing of peat at -20° C for 72 h, the extraction of water, heated to 90° C for 2 h, when the ratio of raw material:solvent 1:12-15, filtration, after which the extract is evaporated to 1/5 of the volume, and purification of the target product by precipitation with ethyl alcohol (EN, p. No. 2036647 And 61 To 35/10).

This way the main disadvantage inherent characteristic of the first method, namely, obtaining the extract with low biological activity. Reduction of biological activity contributes to the high temperatures of 90° extraction, in which a piece of biologically active substances is destroyed.

The closest technical solution to the claimed is a method of obtaining an aqueous extract (stock solution of sodium HUMATE) of peat, including extraction of milled peat in alkaline - aqueous solution of NaOH at a temperature of 80-90° and With periodic stirring for 1 hour, drain the finished product in cumulative capacity by centrifugation followed by settling it within 3-4 hours (EN, p. No. 2177021, C 10 F 7/00).

The main disadvantages of this method are similar to the disadvantages of the first and second counterparts. In addition, a significant drawback is the fact as an extractant is used an aqueous solution of NaOH, that is, carry out acid-base hydrolysis, causing strong, destructive physical and chemical impacts on the native structure fractions of humic compounds (FGS).

The technical result of the present invention is the production of ecologically pure, concentrated extract of mud, with high biological activity.

The technical result is achieved in that a method of obtaining an aqueous extract of mud includes the extraction of fine mud in an alkaline medium at elevated temperature and vigorous stirring to obtain a homogeneous suspension and subsequent separation, the mud used peat mud with the degree of decomposition of plant residues 50% or more, and the extraction was carried out 7-9 amounts of sodium bicarbonate mineral water at a temperature of 65-70° With the separation of the suspension is carried out by settling within 36-48 hours with the separation of liquid phase and subsequent filtration of the liquid phase through a fine mesh filter and centrifuging it at a speed of 2500-3000 rpm

In the present method uses peat mud with the degree of decomposition of plant residues 50% or more extraction which is carried out at a temperature of 65-70° C.

It is this highly mineralized peat and rich'or is adicheskii substances with a high content of aromatic carbon atoms.

The extraction of such peat natural sodium bicarbonate mineral water type “Khabarovsk 1” fully extracted pantielacey hydrocarbon With30H50related to a new class of triterpenoids, which is a new allotropic form of carbon and is included in the so-called fullerene class materials or preparations, which also feature a high degree of aromaticity, which increases the biological activity of the finished product. Pantielacey hydrocarbon With30H50only extracted in alkaline medium at pH PH 6-8.

In addition, extraction of the extracted hydrocarbons of different groups of aromatic structures, the high contents of which are confirmed by data from the fragmented structure of the inventive extract of the spectra R13With that obtained in the laboratory of chemistry and wood Irkutsk Institute of chemistry and listed in the table.

The temperature of the extraction of raw materials 65-70° C is the optimal temperature at which eliminates the decomposition and loss of biologically active substances.

The task contributes to carrying out the separation of suspensions in three stages:

- advocacy within 36-48 hours with the separation of liquid phase 36-48 hours is sufficient time for the maximum separation of the entire complex is Biologicheskie active substances from raw materials and achieve a full, the final form of education and interaction of active substances, passing in the finished product, making it more rich and concentrated;

filtering the liquid phase of the suspension through a fine mesh filter after settling and subsequent centrifugation at a speed of 2500-3000 rpm, provide a final separation of raw materials and clearance from any mechanical impurities to produce ecological pure concentrated extract.

The best indicators of modes of process operations - time defending suspension 36-48 hours, the temperature of the extraction 65-70° and the ratio of raw material to the extractant 1:7-9 were determined taking into account the degree of intensity of extraction of the organic component of the raw material and the extractant, since the use of peat raw materials are extracted mainly organic component.

For preparation of aqueous extract of mud as the mud will use native peat mud with the degree of decomposition of 50% or more, and pasta made from dirt above, for example, peat mud paste type “Chinska (TU 9365-014-2698312-2001), field Bichevskaya”, Khabarovsk.

As extractant use sodium bicarbonate mineral water type “Khabarovsk 1”, “Mukhen field”,Khabarovsk (TU 10 RSFSR 363) or any mineral water similar in composition to the above-mentioned mineral water.

The extract is prepared as follows.

Example 1.

In the mixer-extractor put the pasta peat “Chinska and mineral sodium bicarbonate water “Khabarovsk 1” in the ratio of 1:7, is stirred at a temperature of 65° to obtain a homogeneous suspension. The suspension is poured into plastic containers or drums with polyethylene liner, assert within 36-48 hours. Next, the liquid phase of the suspension is poured into another container and filtered through a fine mesh filter, after which it is centrifuged at a speed of 2500-3000 rpm, getting clean without mechanical impurities, concentrated, rich in biologically active substances aqueous extract with the following indicators. Organoleptic and physico-chemical:

1. AppearanceLiquid opaque, dark brown color
2. ImpuritiesNo, small Pets natural sediment, gumina-mineral substances.
CAPAHSpecific peat up to 3 points
4. The measure of concentration of hydrogen ions (PH)6,0-8,0
5. The total concentration of water soluble gumina-mineral ve the EU ETS, g/l3,5-4,0
6. Phenol concentration, ml/lNot more than 0.001
Microbiological: 
1. Permissible limits of microbial contamination 
(TBC)Not more than 1031 g of product
2. Fungi, the fungi CandidaNot more than 1021 g of product
3. Heavy metals (total), including:Not more than 20 mg/kg
- lead5 mg/kg
- arsenic5 mg/kg
- mercury1 mg/kg

DesignationThe content of the structural fragments of qx, % Rel.The range of chemical shifts (CS) δ , ppm, assignment signals
The C=O carbon Atoms karbonilnyh groups4,4220-186
COhinCarbon atoms quinoid groups9,8186-180
(A)HE, (O)'OR carbon Atoms, carboxyl, hard-ester groups15,2185-168
Cap- On Aromatic carbon atoms connected with oxygen atoms17,9164-140
WitharC, H - and N - Thames is installed aromatic carbon atoms 38,4140-103
CLcarbohydratesAnomeric atoms plastoglobuli5,1103-96
Withα,β -O-4The carbon atoms in α -O-4 and β -O-4-linkages “of the lignin component3,693-80
Cho, CH2Of carbon Atoms-CH-O-CH-N-fragments5,580-58
CH3About carbon Atoms metaxylene groups058-54
WithAlaThe carbon atoms of alkyl fragments0,154-20
The degree of aromaticity (fa) fa=Iap/Itotal57164-103

The solid precipitate mud suspensions can be used as a standalone product for skin applications.

Example 2.

The method is carried out as in example 1, only in a mixer-extractor is placed native peat with natural humidity of 60-80% and the degree of decomposition of plant residues 50% or more and 8 parts of mineral water “Khabarovsk 1”, and the stirring of the suspension is carried out at a temperature of 67° C. the Performance of the obtained extract correspond to the indicators of example 1.

Example 3.

The method is carried out according to example 2, only the ratio of raw material: extractant take 1:9, and the stirring of the suspension is carried out at temperature is re 70° C. Performance of the obtained extract correspond to the indicators of example 1.

As a source of raw materials used in this method can also be used mud silt (sapropel) freshwater lakes in the Amur region.

The inventive method allows to obtain ecologically pure, biologically active drug made from natural raw materials and possessing a broad spectrum of action, will find, in the opinion of the author, a wide industrial application.

The method of obtaining an aqueous extract of the mud, including extraction of fine mud in an alkaline medium at elevated temperature and vigorous stirring to obtain a homogeneous suspension and subsequent separation, characterized in that mud use peat mud with the degree of decomposition of plant residues 50% or more, the extraction was carried out 7-9 amounts of sodium bicarbonate mineral water at a temperature of 65-70°With the separation of the suspension is carried out by settling within 36-48 h with the separation of liquid phase and subsequent filtration of the liquid phase through a fine mesh filter and centrifuging it at a speed of 2500-3000 rpm



 

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