Method for production of peloid water extract
FIELD: method for production of bioproducts from native divot peloid with high biological activity.
SUBSTANCE: claimed method includes extraction of fine dispersed divot peloid having plant residual decomposition ratio of 50 % or more with sodium hydrocarbonate-containing water at elevated temperature under intensive agitation to produce homogeneous suspension followed by separation under certain conditions.
EFFECT: ecologically pure concentrated peloid extract with high biological activity.
The invention relates to a method for producing bio-based products, in particular aqueous extracts from native peat mud, and can be widely used in veterinary medicine, medicine, agriculture - crop production, horticulture, livestock, poultry.
A method of obtaining the extract from the sludge of mud, including the grinding of dirt, humidity 50-60% until pasty state, its extraction of 96%ethanol twice at a ratio of 6 to 10 volumes of alcohol per 1 kg of raw material at a temperature of 30-50° and With intensive stirring for 20-25 minutes, defending spirography weight for 3-4 hours, then poured alcohol micelle. In merged micelle add vaseline oil in quantities that provide extract the desired concentration, and the distillation of alcohol are under vacuum 05-07 ATM at a temperature of not higher than 50° With periodic stirring. The remaining extract assert (waste mostly salt water), and then dissolved in liquid paraffin (EN, p. No. 2073999 And 61 To 35/10).
The disadvantage of this method is incomplete extraction of nutrients due to oxidative processes occurring during extraction in alcohol, and subsequent distillation of the alcohol adding paraffin oil having poor absorption and poor extraction decrease in the percentage allocation to extract biologically active substances contributes to insufficient temperature 30-50° C.
A method of obtaining extracts with antifungal activity from lowland ozokeritovogo peat, including the freezing of peat at -20° C for 72 h, the extraction of water, heated to 90° C for 2 h, when the ratio of raw material:solvent 1:12-15, filtration, after which the extract is evaporated to 1/5 of the volume, and purification of the target product by precipitation with ethyl alcohol (EN, p. No. 2036647 And 61 To 35/10).
This way the main disadvantage inherent characteristic of the first method, namely, obtaining the extract with low biological activity. Reduction of biological activity contributes to the high temperatures of 90° extraction, in which a piece of biologically active substances is destroyed.
The closest technical solution to the claimed is a method of obtaining an aqueous extract (stock solution of sodium HUMATE) of peat, including extraction of milled peat in alkaline - aqueous solution of NaOH at a temperature of 80-90° and With periodic stirring for 1 hour, drain the finished product in cumulative capacity by centrifugation followed by settling it within 3-4 hours (EN, p. No. 2177021, C 10 F 7/00).
The main disadvantages of this method are similar to the disadvantages of the first and second counterparts. In addition, a significant drawback is the fact as an extractant is used an aqueous solution of NaOH, that is, carry out acid-base hydrolysis, causing strong, destructive physical and chemical impacts on the native structure fractions of humic compounds (FGS).
The technical result of the present invention is the production of ecologically pure, concentrated extract of mud, with high biological activity.
The technical result is achieved in that a method of obtaining an aqueous extract of mud includes the extraction of fine mud in an alkaline medium at elevated temperature and vigorous stirring to obtain a homogeneous suspension and subsequent separation, the mud used peat mud with the degree of decomposition of plant residues 50% or more, and the extraction was carried out 7-9 amounts of sodium bicarbonate mineral water at a temperature of 65-70° With the separation of the suspension is carried out by settling within 36-48 hours with the separation of liquid phase and subsequent filtration of the liquid phase through a fine mesh filter and centrifuging it at a speed of 2500-3000 rpm
In the present method uses peat mud with the degree of decomposition of plant residues 50% or more extraction which is carried out at a temperature of 65-70° C.
It is this highly mineralized peat and rich'or is adicheskii substances with a high content of aromatic carbon atoms.
The extraction of such peat natural sodium bicarbonate mineral water type “Khabarovsk 1” fully extracted pantielacey hydrocarbon With30H50related to a new class of triterpenoids, which is a new allotropic form of carbon and is included in the so-called fullerene class materials or preparations, which also feature a high degree of aromaticity, which increases the biological activity of the finished product. Pantielacey hydrocarbon With30H50only extracted in alkaline medium at pH PH 6-8.
In addition, extraction of the extracted hydrocarbons of different groups of aromatic structures, the high contents of which are confirmed by data from the fragmented structure of the inventive extract of the spectra R13With that obtained in the laboratory of chemistry and wood Irkutsk Institute of chemistry and listed in the table.
The temperature of the extraction of raw materials 65-70° C is the optimal temperature at which eliminates the decomposition and loss of biologically active substances.
The task contributes to carrying out the separation of suspensions in three stages:
- advocacy within 36-48 hours with the separation of liquid phase 36-48 hours is sufficient time for the maximum separation of the entire complex is Biologicheskie active substances from raw materials and achieve a full, the final form of education and interaction of active substances, passing in the finished product, making it more rich and concentrated;
filtering the liquid phase of the suspension through a fine mesh filter after settling and subsequent centrifugation at a speed of 2500-3000 rpm, provide a final separation of raw materials and clearance from any mechanical impurities to produce ecological pure concentrated extract.
The best indicators of modes of process operations - time defending suspension 36-48 hours, the temperature of the extraction 65-70° and the ratio of raw material to the extractant 1:7-9 were determined taking into account the degree of intensity of extraction of the organic component of the raw material and the extractant, since the use of peat raw materials are extracted mainly organic component.
For preparation of aqueous extract of mud as the mud will use native peat mud with the degree of decomposition of 50% or more, and pasta made from dirt above, for example, peat mud paste type “Chinska (TU 9365-014-2698312-2001), field Bichevskaya”, Khabarovsk.
As extractant use sodium bicarbonate mineral water type “Khabarovsk 1”, “Mukhen field”,Khabarovsk (TU 10 RSFSR 363) or any mineral water similar in composition to the above-mentioned mineral water.
The extract is prepared as follows.
In the mixer-extractor put the pasta peat “Chinska and mineral sodium bicarbonate water “Khabarovsk 1” in the ratio of 1:7, is stirred at a temperature of 65° to obtain a homogeneous suspension. The suspension is poured into plastic containers or drums with polyethylene liner, assert within 36-48 hours. Next, the liquid phase of the suspension is poured into another container and filtered through a fine mesh filter, after which it is centrifuged at a speed of 2500-3000 rpm, getting clean without mechanical impurities, concentrated, rich in biologically active substances aqueous extract with the following indicators. Organoleptic and physico-chemical:
|1. Appearance||Liquid opaque, dark brown color|
|2. Impurities||No, small Pets natural sediment, gumina-mineral substances.|
|CAPAH||Specific peat up to 3 points|
|4. The measure of concentration of hydrogen ions (PH)||6,0-8,0|
|5. The total concentration of water soluble gumina-mineral ve the EU ETS, g/l||3,5-4,0|
|6. Phenol concentration, ml/l||Not more than 0.001|
|1. Permissible limits of microbial contamination|
|(TBC)||Not more than 1031 g of product|
|2. Fungi, the fungi Candida||Not more than 1021 g of product|
|3. Heavy metals (total), including:||Not more than 20 mg/kg|
|- lead||5 mg/kg|
|- arsenic||5 mg/kg|
|- mercury||1 mg/kg|
|Designation||The content of the structural fragments of qx, % Rel.||The range of chemical shifts (CS) δ , ppm, assignment signals|
|The C=O carbon Atoms karbonilnyh groups||4,4||220-186|
|COhinCarbon atoms quinoid groups||9,8||186-180|
|(A)HE, (O)'OR carbon Atoms, carboxyl, hard-ester groups||15,2||185-168|
|Cap- On Aromatic carbon atoms connected with oxygen atoms||17,9||164-140|
|WitharC, H - and N - Thames is installed aromatic carbon atoms||38,4||140-103|
|CLcarbohydratesAnomeric atoms plastoglobuli||5,1||103-96|
|Withα,β -O-4The carbon atoms in α -O-4 and β -O-4-linkages “of the lignin component||3,6||93-80|
|Cho, CH2Of carbon Atoms-CH-O-CH-N-fragments||5,5||80-58|
|CH3About carbon Atoms metaxylene groups||0||58-54|
|WithAlaThe carbon atoms of alkyl fragments||0,1||54-20|
|The degree of aromaticity (fa) fa=Iap/Itotal||57||164-103|
The solid precipitate mud suspensions can be used as a standalone product for skin applications.
The method is carried out as in example 1, only in a mixer-extractor is placed native peat with natural humidity of 60-80% and the degree of decomposition of plant residues 50% or more and 8 parts of mineral water “Khabarovsk 1”, and the stirring of the suspension is carried out at a temperature of 67° C. the Performance of the obtained extract correspond to the indicators of example 1.
The method is carried out according to example 2, only the ratio of raw material: extractant take 1:9, and the stirring of the suspension is carried out at temperature is re 70° C. Performance of the obtained extract correspond to the indicators of example 1.
As a source of raw materials used in this method can also be used mud silt (sapropel) freshwater lakes in the Amur region.
The inventive method allows to obtain ecologically pure, biologically active drug made from natural raw materials and possessing a broad spectrum of action, will find, in the opinion of the author, a wide industrial application.
The method of obtaining an aqueous extract of the mud, including extraction of fine mud in an alkaline medium at elevated temperature and vigorous stirring to obtain a homogeneous suspension and subsequent separation, characterized in that mud use peat mud with the degree of decomposition of plant residues 50% or more, the extraction was carried out 7-9 amounts of sodium bicarbonate mineral water at a temperature of 65-70°With the separation of the suspension is carried out by settling within 36-48 h with the separation of liquid phase and subsequent filtration of the liquid phase through a fine mesh filter and centrifuging it at a speed of 2500-3000 rpm
FIELD: medicine, phthisiology.
SUBSTANCE: in early period the preparation "Yantar-antitoks" is used in the dose 0.5 g, 3 times per a day for one month in addition to the conventional antituberculosis therapy. Invention promotes to elimination of symptoms associated with the total intoxication, resorption of focus-infiltration changes, elimination of destructions and ceasing secretion of microorganisms for shorter periods. Invention can be used in treatment of pulmonary tuberculosis.
EFFECT: improved method for treatment.
4 tbl, 1 ex
FIELD: medicine, reflexotherapy.
SUBSTANCE: the method deals with applying a capsule being opened at its one side upon reflector areas. The capsule contains bee wax, propolis, arenaceous quartz, extract of vegetable raw material. One should apply alcoholic extract of vegetable raw material including sage, St. John's wort, Artemisia, dendritic aloe, oak bark, shelf fungus, peppermint, lovage and immortelle. The capsule should be applied successively onto four reflector areas: solar plexus, cervico-thoracic department of vertebral column, lumbus and the upper left part of thorax to be kept there for about 2-8 h at interval between seances being 4-6 h. The method suggested causes no side effects.
EFFECT: higher efficiency.
1 cl, 2 dwg, 3 ex
FIELD: veterinary science, animal science.
SUBSTANCE: one should introduce lygfol at the dosage of 5 ml/animal, twice, in the end of dry period and during the day of calving, moreover, for the first time lygfol should be introduced 1-2 wk before calving. The present innovation enables to decrease sickness rate and increase reproductive capacity in cattle.
EFFECT: higher efficiency of prophylaxis.
1 cl, 1 tbl
FIELD: prophylactic medicine.
SUBSTANCE: the present innovation deals with curative-prophylactic composition including soybean phospholipid complex being 40-88%, where the content of phosphatidyl choline corresponds to 40-80%, and one or several dry extracts of medicinal plants containing substances of phenolic origin, and their weight ratio to phospholipid complex varies within 1:8 to 1:2, and, also, a dietary biologically active additive based upon the above-mentioned composition.
EFFECT: higher efficiency.
6 cl, 2 ex, 6 tbl
FIELD: chemico-pharmaceutical industry.
SUBSTANCE: the present innovation deals with developing preparation with wide spectrum of pharmacological action based upon medicinal raw material. This preparation is of hepatoprotective, antioxidizing, cappilaroprotective and gastroprotective action being phytocomplex out of larch bark including the mixture of phenoloacids, flavonoids, oligomeric and polymeric proanthocyanidines at the ratio of 1:1:3:5, correspondingly. The preparation could be applied for preventing and treating a lot of diseases accompanied by oxidizing stress.
EFFECT: higher efficiency of prophylaxis and therapy.
6 ex, 6 tbl
SUBSTANCE: the method deals with selecting primary raw material, followed by acidic-alkaline treatment, cutting, forming semi-finished ligatures, followed by drying, polishing and packing into ampoules or parcels, followed by sealing and sterilization. As primary raw material one should predominantly apply porcine viscera followed by elution of ballast substances with1.3 dimethylketone, treatment with succinic anhydride, then comes cutting for, at least, 2-4 parts to be sterilized in dry water-free state at no any preliminary conservation with predominantly radiation emission followed by material rehydration and packing into individual parcels, then comes conservation and sealing individual packing with sterile ligatures. Rehydration of ligatures should be performed, for example, for 16 h in 0.02% solution of (1.2-ethylene-bis N diethylcarbodecynoxymethyl) ammonium chloride. Power of radiation emission at sterilization corresponds to at least 15 kGy, as for polishing, it is predominantly fulfilled due to "drilling". Simultaneously one should perform group sterilization of ligatures without individual packing followed by packing the sterile material. Group sterilization of suture material should be performed without individual packing with gas method of ethylenoxide, the mixture of ethylenoxide with methyl bromide or beta-propiolactone followed by packing the sterile material. The method provides considerable decrease of production cost price, decreased expenses of primary raw material, improved histocompatibility, decreased allergic and purulent-inflammatory complications and keeping strong parameters during sterilization.
EFFECT: higher efficiency.
5 cl, 3 ex, 1 tbl
FIELD: cosmetic industry.
SUBSTANCE: the present innovation deals with cosmetic preparations for nourishing and protecting one's skin. The suggested cosmetic preparation contains vegetable oil and extracts of plant raw material, additionally, it contains carbon dioxide extracts out of propolis and bee wax and, also, it may contain ramson juice, aqueous extract out of fir's woody greens or its bark and, also, honey, chalk and fatty foundation, moreover, components should be taken at a certain quantitative ratio. Method to obtain this cosmetic preparation deals with mixing fatty foundation with biologically active substances to obtain homogeneous mass, moreover, biologically active substances should be added into the fatty foundation at 18-22 C. The suggested cosmetic preparation enables to heal small scratches, excoriations, pustules and acne due to its antiphlogistic action. It should be, also, applied during body massage being of antioxidizing action, as well.
EFFECT: higher efficiency of application.
6 cl, 2 ex, 2 tbl
FIELD: medicine, colonohydrotherapy.
SUBSTANCE: one should perform 3-10 procedures of colonohydrotherapy due to injecting curative solution at the rate of 30-35 l/h. Solution should be obtained by diluting, at least, the powder of 1 sublimated juice and/or extract of plant origin in water at 40 C up to concentration ranged 2.5-20 g/l water, by keeping for 20-25 min, straining and cooling up to 35-37 C. The method enables to perform colonohydrotherapeutic procedures causing no discomfort state and affect the whole body more efficiently.
EFFECT: higher efficiency of body healing.
1 cl, 1 dwg, 3 ex, 1 tbl
FIELD: medicine, pharmaceutics.
SUBSTANCE: raw soft roe should be reduced, treated at certain temperature with 0.2%-acetic acid solution, dehydrated with alcohol, dried to obtain granules, granules should be extracted with sulfuric acid solution at three stages, proteins should be precipitated out of extract by adding triple volume of ethanol, residue should be dissolved, solution should be treated with barium hydroxide up to pH being 7.0-7.5 followed by addition of ammonia up to pH being 10.5-11.5 to separate the residue due to decanting and centrifuging. Solution obtained after decanting and centrifugate should be treated with kieselguhr at heating up to 80 C and filtered upon a Nutch filter, one should apply filtrate through cationite in "H+"-form, eluate should be concentrated due to vacuum evaporation or by applying baromembranous technique, then one should precipitate the product out of eluate with triple volume of alcohol due to a 5-fold reprecipitation along with centrifuging and drying the residue to remove residual moisture and dry ready-to-use product at 55-65 C for 24 h. The present innovation enables to increase the degree of purification of protamine sulfate due to purifying against accompanying foreign proteins.
EFFECT: increased anti-heparin activity.
SUBSTANCE: the present innovation deals with insulin preparations applied in therapy of diabetes mellitus. This innovation could be applied in medicinal industry for insulin manufacturing. To obtain insulin one should apply reindeer's pancreas to be homogenized in solution of hydrochloric acid ethanol followed by extraction with subsequent clarification of solution and obtaining the supernatant which then should undergo ion-exchange chromatography and isoelectric deposition by obtaining insulin. The latter should be purified due to high-performance reversed-phase liquid chromatography. Insulin obtained is competitive for the bond with insulin receptor at concentration of above 100 ng/ml due to causing high increase of receptor binding, moreover, it has higher hydrophoby against standard insulins, thus, it has certain differences in the structure of its molecule.
EFFECT: higher efficiency of insulin manufacturing.
2 cl, 2 dwg, 2 ex
FIELD: medicine, colonohydrotherapy.
SUBSTANCE: one should perform 3-5 procedures of deep intestinal purification due to its irrigating with Carlsbad salt solution and additional supply of ozone or oxygen. One should apply 1-2%-Carlsbad salt solution and perform 3-5 procedures at solution's temperature being 30-36.6 C. The method enables to carry out colonohydrotherapeutic procedures by causing no discomfort body state and affect the whole body more efficiently.
EFFECT: higher efficiency of body healing.
1 cl, 1 dwg, 2 ex, 1 tbl
FIELD: medicine, otorhinolaryngological surgery.
SUBSTANCE: one should apply thin layer of "Solcoseryl" gel onto osseous facial walls of frontal and maxillary sinuses at the border with trepanation opening after removing pathological content out of them and before applying a transplant out of flat bone of human fetal cranial arch that exceeds the diameter of trepanation opening by 3-4 mm. Then, one should additionally fix the transplant by affecting with distal edge part of a light guide of semi-conductor laser "ATKUS-15" with contact-type technique at output power of laser radiation being 8 W at constant mode. The method enables to increase fixation density of allobrefobone to osseous walls of sinus along its whole diameter.
EFFECT: higher efficiency of fixation.