Method for normalizing immunobiochemical homeostasis in cows both at prenatal and parturient periods

FIELD: veterinary science.

SUBSTANCE: about 20-25 d before calving one should introduce intramuscularly 0.5%-sodium selenite solution for cows at the dosage of 10 ml. Twice before and twice after calving at 10-d-long interval - tetravit at the dosage of 10 ml at the content of 50000 IU vitamin A, 25000 IU vitamin D, 20 mg vitamin E and 5 mg vitamin F per 1 ml. Succinic acid should be introduced 20-25 d both before and after calving at the dosage of 1.0 g. The method provides efficient correction of the main values of homeostasis in cows after calving.

EFFECT: higher efficiency of normalization.

2 ex, 4 tbl

 

The invention relates to veterinary medicine, relates to a method of normalizing immunobiochemical homeostasis in cows during pregnancy and in the postpartum period and can be used for the treatment and prevention of cow protection from mass diseases of the reproductive organs and get vysokogradientnogo offspring.

Studies show that numerous stress and intensive use of highly productive cows, usually accompanied by functional disorders of the organs of reproduction and their high morbidity during pregnancy, delivery and the postpartum period. According to some authors, obstetric pathology in these physiological periods of reproduction can take on a mass character and is accompanied by a decrease in reproductive function. So, birth and postpartum complications in cows reduce their fertility 17.7-26.0 per cent, increase the duration of infertility for 42 days, reduce the output of offspring 12-18% (1).

In the development of obstetric pathology plays an important role state immunobiochemical homeostasis, in particular cellular and humoral immunity and natural resistance. Thus, it is noted that on the eve and on the day of calving in cows decreases the number of T - and b-lymphocytes, especially in pathological hotel, after which pomerado the second period of their level recovers slowly (2).

The basis of the etiopathogenesis of massive obstetric pathology in cows is the state of stress maladjustment, accompanied by hyperactivity peroxidation (free-radical) oxidation of lipids (Pol), decreased activity of the antioxidant defense system (AOD) and immunological resistance associated with the immunosuppressive effect of food on the FLOOR. The start-up period and calving is an powerful stressors to the body of pregnant cows, accompanied by activation of peroxidation processes and reduce system activity AOD. The level of toxic peroxidation products (diene conjugates, kettani, malonic aldehyde) in the prenatal period are characterized by high performance and system activity AOD, as in enzymatic (catalase, glutathione peroxidase)and nonenzymatic (vitamins a, E, C) level is low. After calving, cows indicators peroxidation products increase even more sharply, and indicators of the system of AOD reduced.

Currently, the excessive accumulation of waste products in the body FLOOR is considered as one of the leading universal mechanisms of cellular damage as a result of violations of structural-functional organization of biomembranes.

Therefore, excessive accumulation in the body of toxic products FLOOR in the prenatal and early postnatal periods is what I based development chain structural and metabolic changes contributing to the emergence and development of postpartum endometritis (violation ionic equilibrium between the cell and the external environment violates the mechanisms of excitation and contraction of the myometrium, and damage of endothelial cells and depolymerization of hyaluronic acid reduces their protective role and creates the conditions for microflora).

The status value immunobiochemical homeostasis as a pathogenetic factor of obstetric pathology cows indicated by the low efficiency of methods of prophylaxis with the use of drugs excluding immunobiochemistry processes (3).

Correction immunobiochemical homeostasis is carried out with the use of biologically active substances, such as adaptogenic, anti-stress agents, antioxidants. Of antioxidants especially widely used inorganic and organic selenium compounds and preparations on their basis as ultramicrotome to feed (4). So, there is a method of prevention of postpartum diseases in cows, including two for 40-50 days before and one month after the first injection, intramuscular injection of 10 ml of 0.5%aqueous solution of selenium with simultaneous introduction into the diet of complex micro - and macroelements, which increases resistance of the organism of cows (5). Also known correction method pathologies about the Jena substances and the treatment and prevention of its consequences, including the use of the universal complex preparation containing sodium Selenite, vitamin E, succinic acid, zinc sulfate and formazin, which is injected intramuscularly 45, 30, 15 days prior to delivery and on the day of delivery (6). However, the known methods in the complexity of their implementation do not provide normalization immunobiochemical homeostasis in full, acting on his individual performance without taking into account the mechanisms of metabolic disorders depending on the physiological state of the animal, the most important of which is the increased tension in metabolic processes, accompanied by the accumulation of toxic products in the FLOOR in the prenatal period and a significant increase in their level after calving. In these physiological periods of reproduction is the heavy use of plastic compounds tissues, especially fat, further structural lipids (6). Mobilization of fatty acids as a result of violations of recycling in the adjoint their oxidation reactions with carbohydrates causes blood gets oxidized compounds that amid weakening system AOD because of multiple stresses and intensive use creates favorable conditions for activation of nonenzymatic lipid oxidation and as a result, inhibition of cellular and humoral factors of immunity.

This goal is achieved by a complex of optimally selected biologically active agents with antioxidant activity and their application in the original scheme.

The method is as follows.

Cows for 20-25 days before calving once intramuscularly with a 0.5% solution of sodium Selenite at a dose of 10 ml, twice before and twice after calving interval 10 days - tetravit in the dose of 10 ml containing 1 ml of 50,000 IU of vitamin a 25000 IU of vitamin D, 20 mg of vitamin E and 5 mg of vitamin F for 20-25 days before and after calving inside succinic acid at a dose of 1.0,

The feasibility of the method was determined in the research and production experience in the cows of black-motley breed milk yield 3500-3800 kg in terms of one of the farms of the Nizhny Novgorod region with high (>90%) incidence of reproductive organs after calving.

Before conducting experience and 2-3 days after calving from 10 cows each group took blood samples for assessment immunobiochemical homeostasis in hemoglobin, red blood cells, total protein serum and protein fractions (determined by standard methods), glucose (determined ortho-toluidine method),total lipids (determined by the method Krinitsky modification Volgina /1969/), products FLOOR - diene conjugates and ketodienes (determined by the method of Placenta /1976/), malondialdehyde (determined in the reaction with thiobarbituric acid), lysozyme activity of blood serum (determined by the method Vgetarian), and bactericidal activity of blood serum (determined by the method Overmaas and Casesmini), phagocytic activity of blood neutrophils (determined by the method Ushastaja), catalase activity (determined in the reaction with ammonium molybdate), vitamin a /retinol/ (was determined in the reaction with antimony trichloride), vitamin E /tocopherol/ (determined by the method of Bieri modification Rusticelli, Chisquare /1972/).

The essence of the method is illustrated by examples.

Example 1. To study the effect of various biologically active agents with antioxidant activity (of sodium Selenite in the form of a 0.5% solution, terravita and succinic acid) on the FLOOR processes and the state immunobiochemical homeostasis principle analogues formed 4 groups globorotalia cows 10 goals each. Cows first experimental group for 20-25 days before calving once was injected intramuscularly with a 0.5% solution of sodium Selenite at a dose of 10 ml, the cows of the second experimental group within 20-25 days before and after calving gave inside succinic acid at a dose of 1.0 g, cows of the third experimental group for 20-25 days before and in the first 20 DN is th after calving was injected intramuscularly tetravit with an interval of 10 days. Cows fourth group of drugs were used, they were used as control. The research results are summarized in tables 1 and 2.

Table 1

The effect of various antioxidants on the performance of LPO products and systems AOD in cows
IndicatorsGroup of cows
1-I, n=10 goal.

(sodium Selenite)
2-I, n=10 goal. (succinic acid)3-I, n=10 goal. (tetravit)4-I, n=10 goal. (without drugs)
M±m±% M±m±% M±m±% M±m±%
Diene conjugates, units/ml+16,7+8,733,3+63,6
Kettani, units/ml+18,2+12,7+54,6 +75,0
Malonic dialdehyde, umol/l+1,5+1,5+9,9+32,4
Catalase, Mkat/l-10,2-15,4-18,2-49,2
Vitamin a, Μmol/l-32,6-31,7-12,6-57,0
Vitamin E, Mmol/l-26,5-24,6-18,4-45,1
Note: numerator - indicators for 20-25 days before calving

the denominator of indicators 2-3 days after calving

Table 2

The effect of various antioxidants on the state immunobiochemical homeostasis cows
IndicatorsGroup of cows
1-I, n=10 goal (sodium Selenite)2-I, n=10 goal (succinic acid)3-I, n=10 goal (tetravit)4-I, n=10 goal (without drugs)
M±m±% M±m±% M±m±% M±m±%
Hemoglobin, g/l+20,8+19,5+7,45+2,4
Erythrocytes, 1012/l+9,4+9,3+9,6+1,9
Total protein serum, g/l +9,3+6,5+5,9+1,1
Gamma globulin, %+18,3+21,2+14,9-12,0
Glucose, mmol/l+20,5+20,7-21,4-47,4
Total lipids, g/l-13,7is 12.5margin of 13.3+4,2
The authors estimated the effect activity, %+21,9+27,3+17,7 -42,9
Bactericidal activity, %+14,8+13,4+13,9is 7.3
Phagocytic activity of neutrophils, %+8,0+7,2+9,8-15,0
Note: numerator - indicators for 20-25 days before calving

the denominator of indicators 2-3 days after calving

Performance products FLOOR - diene conjugates, kettani and malonic dialdehyde - cows for 20-25 days before calving was characterized by high levels above the physiological norm for this period of 1.59-1,74, 1,08-1.1 and 4,49 with 4.64 times, respectively. Indicators of the system of AOD were reduced in non-enzymatic (vitamin a and E)and enzymatic (catalase) link. 2-3 days after calving performance products FLOOR - diene conjugates, kettani and malonic dialdehyde is significantly increased and exceeded the physiological norm for ManagerID at 1.2-1.8, 1,1-1,29 and 2,08-2.73 times, respectively, as indicators of the system of AOD opposite decreased (table 1). Depending on the applied means less performance peroxidation was increased in the following order: the second group of succinic acid, the first group - sodium Selenite, the third group - tetravit, indicating that the decrease in the intensity of peroxidation processes under the influence of the tested tools.

State immunobiochemical homeostasis in cows for 20-25 days before calving was characterized by a reduced number of indicators below the physiological norm - hemoglobin 3.3 5.6 per cent, the number of erythrocytes by 10-13%, gamma-globulin 4.4-8.8%, which indicated the presence of hypoxia and reduced immune resistance and glucose to the lower limits of physiological norm decrease the intensity of the processes of glycolysis and bioenergetic processes. Heightened levels of total lipids due to metabolic disorders of lipoid compounds that can lead to the breakdown of the synthesis of steroid hormones and the violation of the involutionary process of the reproductive organs after calving. Low rates lysozyme, and bactericidal activity of blood serum and phagocytic activity of neutrophils in the blood is evidence of decreased natural resistance dead short the century After calving in cows of the control group showed a dramatic downturn in natural resistance and glucose. In cows of the experimental groups showed a trend toward normalization of immunobiochemical homeostasis, as evidenced by the increase of the main indicators of the content of hemoglobin, number of erythrocytes, the total protein content of blood serum, the number of gamma-globulin, glucose, Lisitsina, bactericidal activity of blood serum and phagocytic activity of blood neutrophils (table 2).

Example 2. To study the effect of different combinations of biologically active agents with antioxidant activity (0.5% solution of sodium Selenite, terravita and succinic acid) and their application to the processes of GENDER and status immunobiochemical homeostasis principle analogues formed 4 groups globorotalia cows. Cows first group (32 goal.) for 20-25 days before calving used a single intramuscular injection of 0.5% solution of sodium Selenite at a dose of 10 ml, intramuscular injection of terravita in the dose of 10 ml twice with an interval of 10 days before and after calving and within 20-25 days before and after calving inside succinic acid at a dose of 1.0, the Cows in the second group (27 goal.) for 20-25 days before calving used a single intramuscular injection of 0.5% solution of sodium Selenite at a dose of 10 ml, intramuscular injection t is travita in the dose of 10 ml twice with an interval of 10 days before and after calving. Cows of the third group (10 animals) drugs were not used, they served as a control. The results are presented in tables 3 and 4.

The combined use of antioxidants provided the normalization immunobiochemical homeostasis (the combined use of sodium Selenite and terravita up to the level of the lower limits of physiological norm, the combined use of sodium Selenite, terravita and succinic acid to the level of the average limits of physiological norm) due to the decrease in the levels of LPO products and system activation AOD (tables 3 and 4).

Table 3

The effect of combinations of various antioxidants on the performance of LPO products and systems AOD in cows
IndicatorsGroups of animals
1-I, n=33 goal. (sodium Selenite + tetravit + succinic acid)2-I, n=27 goal. (sodium Selenite + tetravit)3-I, n=10 goal. (without drugs)
M±m±% M±m±% M±m±%
Diene conjugates, units/ml-46,6+9,7 +40,7
Kettani, units/ml-20,7+7,0+61,8
Malonic dialdehyde, umol/l-20,4+2,2+27,9
Catalase, mkat/l+6,5+3,7-29,4
Vitamin a, µmol/l+12,3+5,0--33,5
Vitamin E, mmol/l+10,7+1,4-26,0
Note: numerator - indicators for 20-25 days before calving

the denominator of indicators 2-3 days after calving

Table 4

The effect of combinations of various antioxidants on the state immunobiochemical homeostasis cows

IndicatorsGroups of animals
1-I, n=33 goal (sodium Selenite + tetravit + succinic acid)2-I, n=27 goal (sodium Selenite + tetravit)3-I, n=10 goal (without drugs)
M±m±% M±m±% M±m±%
Hemoglobin, g/l+13,5+11,5+3,1
Erythrocytes, 1012/l+15,4+10,9+1,9
Total protein serum, g/l+7,1+4,4-2,3
Gamma-globulins, % +32,5+12,7-7,2
Glucose, mmol/l+16,3+14,7-22,6
Total lipids, g/l-18,0-14,0+3,2
The authors estimated the effect activity, %+38,0+22,5-41,5
Bactericidal activity, %+23,6+21,0-5,8
Phagocytic activity of neutrophils, %+16,4+9,6-1,7
Note: numerator - indicators for 20-25 days before calving

the denominator of indicators 2-3 days after calving

The data obtained confirmed the achievement of the claimed technical result is the normalization immunobiochemical homeostasis concomitant use of sodium Selenite, terravita and succinic acid stated in the scheme. When comparing the dynamics of changes in indicators of toxic products FLOOR system AOD and immunobiochemical homeostasis under the influence of certain antioxidants or combinations thereof observed a positive trend, which was reflected in the decrease in LPO products and improve system AOD and immunobiochemical homeostasis. However, if antioxidants were mainly raised some indicators immunobiochemical homeostasis and reduced the growth of the level of LPO products, the combination of a less pronounced impact on some indicators immunobiochemical homeostasis provided a sharp decrease in the level of toxic products FLOOR and as a result, increased system activity AOD and indicators of natural resistance. Increasing the level of natural resistance for the basic parameters to physiological norms creates the necessary basis for effective treatment and prevention for the of ITA's cows from mass diseases of the reproductive organs and get vysokogradientnogo offspring.

Sources of information taken into account:

1. Nezhdanov A.G., Doshukaeva KG Placental insufficiency and its prevention in cows. //Veterinary medicine. - 1999. No. 7. - P.6-11.

2. Yablonsky, VA, Burns CENTURIES Immune status of cows with normal and abnormal calving //veterinary medicine. - 1984. No. 8. - P.50-51.

3. Erokhin A.S., Fedorchenko O.A., Jugs B.C. Prevention of disorders of the reproductive function of cows //veterinary medicine. - 1998. No. 3. - P.37-38.

4. Problems on selenium in animal nutrition. Norw. J. agr. Sci. - 1993. - Suppl. 11. - P.1-216.

5. RF patent №2201221 S2, 2003

6. RF application No. 2001115497 And published. 2003.06.20.

The method of normalization immunobiochemical homeostasis cows in the prenatal and postpartum periods, including the combined use of 0.5% solution of sodium Selenite and active means, characterized in that active funds are used tetravit and succinic acid according to the following scheme: cows for 20-25 days before calving once intramuscularly with a 0.5% solution of sodium Selenite at a dose of 10 ml, twice before and twice after calving interval 10 days - tetravit in the dose of 10 ml containing 1 ml of 50,000 IU of vitamin a 25000 IU of vitamin D, 20 mg of vitamin E and 5 mg of vitamin F in for 20-25 days before and after calving inside succinic acid at a dose of 1.0,



 

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