Method for complex evaluating the severity of hemodynamic and metabolic disorders in women at perimenopausal period

FIELD: medicine, cardiology, endocrinology, gynecology.

SUBSTANCE: one should detect informational-valuable signs of patient's state, such as either the presence or absence of hypertonic disease and uterine extirpation together with adnexa, the value of body weight index, predominance of disorders according to modified menopausal index (MMI)such as autonomic, metabolic-endocrine or psycho-emotional ones, the type of metabolic structures of blood serum, moreover, it is necessary to echocardiographically detect stroke volume, cardiac index and systemic vascular resistance (SVR), at ultrasound testing one should detect maximal linear rate of circulation (LRC max) by medial cerebral artery and thyroid alterations, rheovasographically one should detect specific circulation (SC) of shins, at testing laser doppler flowmetry one should detect microcirculation index, biochemically it is necessary to detect the value of beta-adrenoreactivity, cholesterol level and that of B-lipoproteides, crystallographically - the presence of serotonin and dopamine crystals, due to immunoenzymatic assay on should detect the values by Table 1 and then after obtaining the values of diagnostic coefficients of every parameter it is necessary to summarize them and obtain diagnostic index (DI), at its value being below 10 one should state no alteration, at its value 10-10 - undetermined state, at its value being 21-30 - the 2nd severity degree of disorders, and at DI value being above 31 one should state the 3d severity degree of disorders available.

EFFECT: higher accuracy of evaluation.

5 ex, 2 tbl

 

The alleged invention relates to medicine and can be used in therapy, cardiology, endocrinology, and gynecology.

As the disappearance of a protective effect of estrogen on the cardiovascular system in postmenopausal women, as well as in connection with age-related changes progressively increased frequency of cardiovascular disease (Smetnik V.P. et al. 2000). According to Tea with co-authors (1989), the level of cardiovascular disease is increased from 8.2% in reproductive age 52.3% of the menopause. Premature death from cardiovascular diseases in the age of 45-54 years will result in an average loss of 20 years of life for every 100 women. However, there is a lack of information about the characteristics of diagnostic tests, the effectiveness of medical and surgical treatment due to the rare women's participation in research programs. The results of research conducted mainly on the male part of the population, do not take into account the characteristics of the female body. This may be one of the reasons for the poor quality of diagnosis and the low efficiency of the treatment of some cardiovascular diseases, including hypertension, myocardial infarction, angina pectoris, and some types of cardiomyopathy. At the same time, there are no specific criteria the severity of the cardiovascular system in conditions of estrogen deficiency, not developed a way of assessing the severity of the condition of women in the perimenopausal period.

The known method of computing diagnostics threatening conditions (Gubler E.V. Computational methods of analysis and recognition of pathological processes, 1978, 1990) using mathematical calculations (point method of diagnosis). For this purpose, the diagnostic table, including a certain number of characteristics. Each sign is divided into ranges (2-16). Then calculate the frequencies (number of observations) in each band for each of the diseases A1 and A2. Further, by determining the amount of observations A1 and A2, take each of them for 100% and calculate the relative P in each band. Then calculate the relationship of chastoty and the logarithm relationship chastoty multiplied by 5, i.e. a diagnostic factor. The next step is the calculation of information content (J) of the sign by the formula J(xj)=5 lg P (xj /A1)/P(xj/A2)·0,5[P(xj/A1)-P(xj/A2)].

Informative considered signs with a value of J=0.5 and more.

Then be formalized information form to assess the severity of the condition and make there an informative signs with ranges and diagnostic factors, which further make a diagnosis and assess the severity of the patient.

The method does not allow for assessment of the severity of hemodynamic and metabolicsyndrome women in the perimenopausal period.

There is a method of assessing the severity of the condition in women of perimenopausal period by the number of “tides” heat (Crimean M. “Menopause”, 1989). On this basis, I propose to distinguish between the severity of mild, moderate and severe menopausal syndrome. Emphasizing the contingent nature of this division, propose to establish a diagnosis of “mild menopausal syndrome patients, who have up to 10 “tides” in the day when this overall condition has not changed. When the form of climacteric syndrome of moderate severity, the patient comprise 10-20 “tides per day, in addition she complains of dizziness, headache, memory impairment. In severe menopausal syndrome, hot flashes occur up to 10-15 per hour, in addition the patient identify the symptoms characteristic of vegetative-vascular, metabolic, endocrine and nutritional disorders. Disadvantages: this method does not allow to obtain a reliable assessment of the status of women in the perimenopausal period, therefore, there could be the wrong approach to fixing the hemodynamic and metabolic abnormalities in this period.

For the prototype we have adopted a modified menopausal index (MMI) of Kupperman modification Everway (Valeeva E.M. Guide the endocrine gineco is ogie”, 1998). This index allows us to analyze the severity of various symptoms of menopausal syndrome: neuro, neurological and somatic, and thus determine the presence or absence of hypertension, the rate of body mass index, hormone levels, ultrasound examination of the thyroid gland. When calculating MMI produce symptoms in a specific sequence: blood pressure, hot flushes, sweating, palpitations, etc. Each of the individual symptoms assessed depending on the severity of scores from 0 to 3. The selected symptom analyze separately. The value of neuro-syndromic, assessed up to 10 points, considered as the absence of clinical manifestations; 10-20 points - as a weak degree of impairment; 21-30 points - average; more than 30 points - how severe form of the syndrome. Metabolic-endocrine and emotional disorders within 1-7 points - weak degree; 8-14 points - average; more than 14 points - severe form of the disease. Disadvantages: this method of assessing the severity of the condition is only for women with climacteric syndrome, however, in women with saline during menopause are also available hemodynamic and metabolic disorders, leading ultimately to the development of the of deseases, such as hypertension, ischemic heart disease, hypothyroidism, etc. So the importance of early diagnosis of these disorders is relevant in patients with pathological and physiological course of menopause.

Tasks:

1. Based on a comprehensive assessment of hemodynamic and metabolic abnormalities in women of perimenopausal period to ensure an accurate assessment of the severity of these violations, provide new diagnostic approaches, tactics of management of patients in the perimenopausal period.

The essence of the invention is that the reveal 18 informative-znajomymi signs J≥0.5) and depending on the extent of violations of the norm, when determining echocardiographic studies reveal the stroke volume of the heart, cardiac index and total peripheral vascular resistance and assign these indicators respectively the values: -3-5; -3-7; 5 to 8; when determining reovazograficheskie studies reveal the specific blood flow to the legs and assign a value-6-5; when determining vascular ultrasound of the brain reveal maximum linear blood flow velocity in middle cerebral artery and assign a value-7-5; when determining the modified menopausal index identify the prevalence of vegetative or metabolic-endocrine, or siamotyrannus violations and assign a value-8-7; by laser Doppler flowmetry determine the index of microcirculation and assign a value-5-5; biochemical method determines the index of beta adrenoreceptor and assign a value of 4-6; kristallograficheski way to detect the presence of crystals of serotonin and dopamine and assign values accordingly 10-6 and-5-9; enzyme-linked immunosorbent assay in hormonal background determine the level of thyroid-stimulating hormone and assign a value of 5-6; levels of cholesterol and lipoproteins assign values 5-9; indicator of body mass index assigns a value of-6-5; when diagnosing hypertension assign a value of +9, and in its absence -2, when transferred removing assign a value +8, and in its absence -2, when determining ultrasound examination of the thyroid gland assign a value-5-5; then optionally identify the type mapping metabolic structures serum and assign a value of 5-10; then determine the diagnostic index for the total value of the signs of the condition and when this index value < -10 define no risk of violations; if the index-10-10 - status uncertain; 11-20 determine 1 the severity of the violations; 21-30 determine 2 the severity of the violations, and if the value of the diagnostic index of 31 and above determine 3 the severity of the violations.

The method is as follows.

The diagnostic table, including 60 characteristics (clinical, laboratory, instrumental and kristallograficheskie indicators). Each sign is divided into ranges (2-5). Then calculate the frequencies (number of observations) in each range when each of the States A1 (corresponds to the perimenopausal period) and A2 (corresponding to postmenopausal period). Further, by determining the amount of observations A1 and A2, take each of them for 100% and calculate the relative P in each band. Then calculate the relationship of chastoty and the logarithm relationship chastoty multiplied by 5, i.e. diagnostic factor.

The next step is the calculation of information content (J) of the sign by the formula J(xj)=5 lg P(xj/A1)/P(xj/A2)·0,5[P(xj/A1)-P(xj/A2)].

Informative considered signs with a value of J=0.5 and more. Get 18 informative features.

Table 1
Signs and their gradesDKSigns and their gradesDKSigns and their gradesYes
1. Show. beta adrenoreceptor, usled8. the body mass index 14. direcrory hormone nmol/l
8-16-0,28<23-5,70,8+1,7
17-40+5,723-25-4,90,8-2,5-4,8
>40was 3.725-30+3,42,5-3+2,07
2. Stroke volume, ml >30+4,5>3+6,6
<70+5,29. Serotonin 15. Ultrasound shields, gland
70-80-2,30+6,3increased0
>80-2,81-2,3reduced+3,2
3. Cardiac index, l/min, *m22-10,6diffuse ISM.+2.1
<3+7,23was 3.7sites+5,2
3-4-2,910. Dopamine No change-4,8
>4-2,30+7,416. Extir the situation of the uterus with appendages
4. The round, DIN*s*cm-51-5,7 
<1120-4,82-4,8there+8,6
1120-1800a-0.73-7,2no-2
>1800+8,611. cholesterol, mmol/l17. mapping of metabolic structures
5. ISC maximum, cm/sec<4-1,7 
<90+5,24-5 .5-5,71 TYPE-5
90-117of-1.45,5-6,5+9,42 type+2
>117-7,2>6,5+7,73 type+5
6. MC Shin, ml/100 g*cm312. In - lipoprotein, g/l4 type+3
<2+4,6<4-1,718. Probed. the violations. on MMG
2,0-2,8-0,34-5,5-5,7  
>2,8-5,75,5-6,5+9,4vegetative.-8
7. Pok. microcirculation, units>6,5+7,7exchange rate - endocr.+7
<4+2,713. Hypertension, diseasepsycho-tip.+3
4-6-5,3there+8,6  
>6+5,2no-2  

Then be formalized information form to assess the severity of the condition and make there an informative signs with ranges and diagnostic factors (DK) (see table 1), which further make a diagnosis and assess the severity of the condition of women.

The patient conduct a comprehensive examination, which includes the determination of 18 the above characteristics (see table 1). After receiving the diagnostic values of the coefficients of each of the indicators summarize them and get a diagnostic index (DI) according to the formula: DI=DK1+DC+DC+DC+DC+...+DK.

Then decide: if value of DI < -10 determine the absence of ri is SC violations; when the value of DI-10-10 - status uncertain; 11-20 determine 1 the severity of the violations; 21-30 determine 2 the severity of the violations, and if the value of the diagnostic index of 31 and above 3 determine the severity of the violations.

Example No. 1. Patient K., 52, has complained of General weakness, hot flashes up to 10 times a day, increased sweating, seizures, palpitations, which occurred about 5 months amid disruptions of the menstrual cycle.

The patient survey was conducted. Received the following: index beta adrenoreceptor =35(+6); index stroke volume of the heart =75(-2); index cardiac index =3,5(-3); total peripheral vascular resistance =1270(0); index linear blood flow velocity in middle cerebral artery =85(+5); specific flow shins =1.8V(+5); microcirculation index =4,5(-5); index the body mass index =26(+3); rate of serotonin =0(+6); rate of dopamine =2(-5); cholesterol of 4.5(-5); rate of beta-lipoproteins =4,2(-5); metric MMG - the prevalence of autonomic disorders (-8); rate of thyroid-stimulating hormone =1(-5); ultrasound examination of the thyroid gland was not carried out (no need); index mapping metabolic structures (+2), hysterectomy and hypertension - there are no (-2) - see table 2.

Table 2
SignsDKSignsDK
1. In-arm, used11. cholesterol, mmol/l
35+64,5-5
2. Stroke volume, ml12. In-lipoproteins, g/l
75-24,2-5
3. Cardiac index13. hypertension
3-3no-2
4. SVR14. TSH, nmol/l
127001-5
5. ISC maximum15. USIMG 
85+5 0
6. MC Shin  
1,8+516. mapping MSK
7. PM, units 2 type+2
4,5-517. Probed. the violations.
8. BMI  Neuro-veget.-8
26+318. Hysterectomy with appendages
9. Serotonin  
0+6 
10. Dopamine no-2
2-5  

DI=6-2-3+0+5+5-5+3+6-5-5-5-2-5+0+2-8-2=-15

After summation of diagnostic ratios (they are shown in parentheses) we received a diagnostic index =-15. At the moment the patient no hemodynamic and metabolic disorders. It has the perimenopausal period.

Example No. 2. Patient N., 49 years, no complaints. In the last 3 months notes disruptions of the menstrual cycle.

As a result of the examination on the above method and the calculation of diagnostic factors in this patient's total diagnostic index =-10, indicating an indeterminate state. In this situation, you control tests in the dynamics.

Example No. 3. Patient C., 55 years. Currently, no complaints, notes the increase of body weight, 4 years ago, I had a hysterectomy with appendages on uterine fibroids.

As a result of the survey and calculation of diagnostic factors in this patient's total diagnostic index =+11, which indicates 1 severity of violations. You need the supervision of a gynecologist, endocrinologist, control tests.

Example No. 4. Patient O., 48 L., AMB. He complained of General weakness, malaise, pressing pain in region of heart, hot flashes up to 10 times a day, adding body mass. From the anamnesis: considers himself to be patient for about 3 years, when there were hot flushes, pain in the heart of the oppressive nature associated with emotional stress. When examined by a gynecologist in 1999 established the diagnosis of uterine fibroids. Treated with norcolut 5 mg. currently, drugs are not accepted.

As a result of the examination, the patient has 2 severity of violations (DI =+21), also had elevated levels of thyroid-stimulating hormone and serotonin in serum, the presence of diffuse changes of a thyroid gland, therefore it needs correction syndrome hypothyroidism, in the dynamics of BP control, the supervision of a gynecologist, endocrinologist.

Example No. 5. Patient A., 51, And/B No. 116, was admitted to the cardiology Department KKB 04.01.2002, in an emergency with complaints of increased AD up to 200/120 mm Hg, accompanied by pain in the region of the heart, headache nausea. Adapted to HELL 120/80 mm Hg From the anamnesis: considers herself a patient since September 2001, when amid the onset of perimenopause (disruptions of the menstrual cycle) were bothered by headaches, General weakness, irritability, tearfulness, there was an increase of HELL to 180/100 mm Hg In October of the same year was outpatient examination in cardiology centre, the ROCKS, where diagnosed with severe arterial hypertension stage 2. Climacteric syndrome of moderate severity. Identified hyperlipidemia. Were treated with antihypertensive drugs, but continued weakness, the minimum numbers of AD 150-160/90 mm Hg Deterioration in the last month: blood pressure rises up to 200/120 mm Hg, with the regular intake of capoten in a daily dose of 75 mg Repeatedly called the ambulance, acute crises. KKB delivered in an emergency in connection with the development of the next hypertensive crisis - increased blood pressure up to 200/100 mm Hg

The survey identified 3 severity of violations (DI =+45).

The patient has a clinical diagnosis of hypertension 2 tbsp. Menopausal period. Syndrome hypothyroidism, mild case, offset, 3 the severity of hemodynamic and metabolic disorders. Should the treatment of arterial hypertension, Hypo is epidemically therapy, correction of hypothyroidism. In the dynamics of control HELL, lipids and thyroid hormones in the blood.

The introduction of materials research will improve the accuracy of assessing the severity of the hemodynamic and metabolic abnormalities in women in the perimenopausal period, to reduce the number of people with severe forms of cardiovascular and endocrine diseases, improve the quality of life for this group of women and to reduce the number of days of incapacity.

The method of complex assessment of the severity of hemodynamic and metabolic abnormalities in women in the perimenopausal period, which includes the determination of the most informative signs of the condition, characterized in that as informative signs of the condition to detect the presence or absence of hypertension and removing the uterus with appendages, the rate of body mass index, prevalence of disorders according to the modified menopausal index (MMI) autonomic, metabolic and endocrine or emotional, metabolic type structures serum, echocardiographically determine the stroke volume of the heart, cardiac index and total peripheral vascular resistance (SVR), an ultrasound revealed the maximum linear blood flow velocity (BFV maximum) in the middle cerebral artery and changes in the thyroid is the gland, reovazograficheskie identify the specific blood flow (QM) of the tibia, in the study of laser Doppler flowmetry determine the index of microcirculation, biochemically determine the measure of beta adrenoreceptor, cholesterol and lipoproteins, kristallograficheski the presence of crystals of serotonin and dopamine, enzyme-linked immunosorbent assay to determine the level of thyroid-stimulating hormone derived indicators assign values from table 1 and then after receiving the diagnostic values of the coefficients of each of the indicators summarize them and get a diagnostic index (DI), a value that is less than 10 determine the absence of violations of, when the value of DI -10÷10 - status uncertain, at 11-20 determine 1 the severity of the violations, when the value 21-30 - 2 severity of violations, and a value of DI 31 and above 3 determine the severity of the violations.



 

Same patents:

FIELD: medicine, anesthesiology, resuscitation.

SUBSTANCE: in patients one should study the content of lactoferrin in peritoneal exudates during the 1st d of postoperational period and at decreased value being below 3500 ng/ml on should predict unfavorable result. The suggested method provides correction of possible postoperational complications that deteriorate the flow of peritonitis and lead to lethal result.

EFFECT: higher accuracy of prediction.

3 ex

FIELD: medicinal immunology.

SUBSTANCE: one should sorb inspecific immunoglobulin G in microplot's holes, then one should successively supplement these holes with solution containing guinea-pig's serum and sodium ethylenediaminotetraacetate and after incubation - an analyzed sample containing Clr2s2 complex of human component at unknown activity and pseudoglobulin fraction of human blood serum obtained due to dialysis against distilled water, and, also, a buffer solution that contains calcium and magnesium ions and after incubation it is necessary to wash and dry the plot, holes should be supplemented with enzymatic conjugate with antibodies against human C3 component and substrate of this enzyme, according to the quantity of formed product of enzymatic reaction one should calculate the activity of Clr2s2 complex.

EFFECT: higher efficiency of detection.

1 ex, 1 tbl

FIELD: medicinal immunology.

SUBSTANCE: the present innovation deals with detecting functional activity of complement components in human blood serum while predicting the row of diseases and in biological preparations, as well. One should sorb immunochemically pure immunoglobulin G in microplot's holes, then it is necessary to supplement the holes with an analyzed sample that contains human complement C3 component at unknown activity, and solution that contains guinea-pig's serum to carry out incubation. Then the content of holes should be poured out to introduce enzymatic conjugate with antibodies against human C3 component and substrate of this enzyme into the holes. C3 activity should be calculated according to the quantity of developed product of enzymatic reaction.

EFFECT: higher sensitivity and shortened terms of detection.

2 dwg, 2 ex

FIELD: clinical biochemistry.

SUBSTANCE: one should detect the concentration of extracellular nucleic acids connected with cell surface of formula blood elements in total fraction obtained due to a two-staged elution of blood cells fraction, and at zero value of this value one should diagnose cancer.

EFFECT: higher significance of diagnostics at early stages.

2 cl, 4 ex, 3 tbl

FIELD: medicine, immunological diagnostics.

SUBSTANCE: in patients' coprofiltrates simultaneously in one diagnostic kit on should detect the presence of erysipelas viral antigen and erysipelas viral antibodies and at antigen content being above 1.3 relative units and antibodies being below 0.30 relative units it is possible to predict the flow of infection during 6-8 d, and at antigen content being above 1.3 relative units and antibodies being above 0.60-0.80 relative units one should predict the flow of infection during 2-3 d. The method enables to predict the development of complications.

EFFECT: higher accuracy of prediction.

2 ex, 1 tbl

FIELD: chemical industry; biological industry.

SUBSTANCE: method and device can be used for testing biological, chemical and biochemical surface sensors which operation is based upon excitation of surface electromagnetic waves on surface of solid body. Change in phase of surface (or waveguide) wave is measured by means of interference between signal wave (waveguide or plasma) propagating along tested surface and volumetric reference wave propagating inside liquid directly above the surface at a small angle to the surface. Both interfering waves pass through liquid cell. One wave has shape of surface (plasma or waveguide) wave, the other one has shape of volumetric wave propagating directly above the surface. Change in volumetric parameters of liquid, for example, refractivity, influences both waves - signal surface and reference volumetric. The influence is mutually compensated in registered interference signal. Sensitivity to volumetric parameters of liquid is reduced while high sensitivity to properties of surface layer is kept.

EFFECT: improved precision.

7 cl, 10 dwg

FIELD: medicine, ophthalmology.

SUBSTANCE: the present innovation deals with detecting clinico-functional parameters by studying patient's lacrima due to crystallographic technique. For this purpose one should measure relative width of peripheral zone of crystallization and detect the availability of transitional area between crystallization zones; and if relative width of peripheral crystallization zone is above one seventh of crystallogram's radius and it is possible to observe transitional area between peripheral and central crystallization zones one should diagnose keratoconus at its early stage.

EFFECT: higher accuracy of diagnostics.

2 dwg, 2 ex

FIELD: biochemical engineering.

SUBSTANCE: set is usable for testing antibodies in tumors, like radioactively labeled anti-CD20-conjugates. The conjugates are used for treating and diagnosing B-cell lymphoma tumors. All reagents are sterile.

EFFECT: wide range of functional applications.

103 cl, 38 dwg, 46 tbl

FIELD: medicine, immunology.

SUBSTANCE: in blood serum of pregnant women one should detect the content of circulating immune complex of myelin as the main protein with immunoglobulin G at pregnancy terms after 20 wk and its values being below 0.1 mcg/ml illustrate uncomplicated pregnancy, the level ranged 0.1 - 0.2 mcg/ml points to preeclampsia of light degree, the level of 0.2-0.5 mcg/ml points to preeclampsia of severe degree. The present innovation provides earlier diagnostics before manifestation of traditional clinico-laboratory data.

EFFECT: higher efficiency of evaluation.

3 ex, 1 tbl

FIELD: medicine, surgery.

SUBSTANCE: one should carry out virological testing patient's blood serum and hepatic bioptates. At detecting TTVDNA and HGVRNA it is necessary to perform ultrasound survey, and at availability of biliary sludge one should conclude upon early stage of cholelithiasis.

EFFECT: higher accuracy of diagnostics.

3 ex

FIELD: medicine, anesthesiology, resuscitation.

SUBSTANCE: in patients one should study the content of lactoferrin in peritoneal exudates during the 1st d of postoperational period and at decreased value being below 3500 ng/ml on should predict unfavorable result. The suggested method provides correction of possible postoperational complications that deteriorate the flow of peritonitis and lead to lethal result.

EFFECT: higher accuracy of prediction.

3 ex

FIELD: medicine.

SUBSTANCE: the present innovation deals with comparing the amplitudes of test curves of pH measurement in mouth cavitary media both before and after application of suggested preparations. Test curves of pH measurement towards acidic side are being obtained after activating acid-forming oral microflora with 15 ml 50%-saccharose solution as oral rinsing for 30 sec, and test curves of pH measurement towards alkaline side are being obtained after activating ammonia-forming microflora with 15 ml 8%-carbamide solution (oral rinsing for 30 sec). One should evaluate the efficiency of such preparations according to the difference of amplitudes for test saccharose and carbamide pH curves (ΔAs and ΔAc) obtained both before and after application of the preparations suggested, total evaluation of preparation impact upon oral microflora should be performed due to comparing the values of ΔAs and ΔAc. The method provides evaluation of preparation impact not only upon acid-producing oral microflora but ammonia-producing one, as well.

EFFECT: higher efficiency and accuracy of evaluation.

1 dwg, 1 ex

FIELD: medicine, oncology.

SUBSTANCE: in patients before treatment one should detect the content of total 17-oxycorticosteroids, cortisol, cortisone, tetrahydrocortisol, tetrahydrocortisone in daily urine to calculate the ratio for the concentration of total 17-oxycorticosteroids to the sum of cortisol, cortisone, tetrahydrocortisol, tetrahydrocortisone concentrations and at ratio value being below 2.0 (1.1-1.6) it is possible to predict the duration of relapse-free period ranged 3-6 yr after the end of complex therapy, and at ratio value being above 2.0 (2.4-3.2) - the development of relapse for terms up to 1.5 yr after therapy conducted. The method provides accuracy, availability and requires no high financial expenses.

EFFECT: higher accuracy of prediction.

2 ex, 1 tbl

FIELD: microbiology, pharmaceutical agents.

SUBSTANCE: invention relates to method for determination of genome response of specific cells to vegetable extract action. Method for drug screening includes 1) treatment of specific cells with vegetable extract; 2) protein or RNA isolation from said treated cells; 3) identification of isolated protein or RNA; 4) determination of compound(s) in said vegetable extract; 5) treatment of said cells with determined compound(s); 6) protein or RNA isolation from said treated cells; and 7) determination of compounds providing expression or suppression of said protein or RNA which have another concentration than in untreated said specific cells.

EFFECT: identification of individual compound(s) for screening of new pharmaceutical agents or new pharmaceutical application of existing drugs.

14 cl

FIELD: medicine.

SUBSTANCE: method involves determining infrared radiation pass band, calculating mean value of infrared radiation band pass coefficient. The value being greater than 12.75 in 3500-3200 cm-1 and less than 35.55 and 46.1 in band passes of 1600-1500 cm-1and 1000-930 cm-1, respectively, chronic tonsillitis is diagnosed under condition that norm of infrared radiation band pass coefficient being less than 12.75 in band passes of 3500-3200 cm-1,greater than 35.55 in band pass of 1600-1500 cm-1 and from 46.1 to 54.9 and from 1000 to 930 cm-1.

EFFECT: diagnosis objectiveness.

FIELD: medicine.

SUBSTANCE: method involves checking consciousness, blood coagulation state, peripheral blood leukocytes number, K+ ions, bilirubin, fibrinogen, hemolysis and hemoglobinuria availability, prothrombin index and exotoxic shock development. Each value is calculated in points as follows. Lucidity is evaluated as -2 points; depression - +3 points; coma - +6 points; lack of changes in blood coagulation system - -2 points; coagulation availability without clinical injuries - +2 points; coagulopathy with clinical manifestation signs - +19 points; K+ ions concentration being less than 3.0 mmole/l - +3 points, from 3.1 to 3.5 mmole/l - -5 points, from 3.6 to 5.0 mmole/l - 0 points, greater than 5.0 points - +7 points, failure in determining K+ ions concentration - 0 points; hemolysis availability - +6 points, its lack - -3 points; hemoglobinuria availability - +8 points, its lack - -1 points; leukocytes number being less than 12.0x109/l - -2 points, from 12,1 to 18.0x109/l - 0 points, higher than 18.0x109/l - +8 points; hourly urine output being less than 30 ml/h - +6 points, greater than 30 ml/h - -2 points; bilirubin content being less than 31 mcmole/l - -2 points, from 30.1 to 50.0 mcmole/l - 0 points, greater than 50.0 mcmole/l - +2 points, failure in determining bilirubin content due to hemolysis being available -+6 points; prothrombin index being equal to or less than 60% - +3 points, greater than 60% - 0 points, failure in determining prothrombin index due to hemolysis being available - +12 points; fibrinogen concentration in blood plasma being less than 2.1 g/l - +4 points, from 2.1 to 4.0 g/l - -1 point, from 4.1 to 6.0 g/l - +1 point, failure in determining fibrinogen concentration due to erythrocyte hemolysis being available - +13 points; exotoxic shock development - +9 points, its lack - -1 point. The points are summed up. The value being greater than +13, admission for treatment in resuscitation department is indicated. The value being less than -13, admission for treatment in therapeutics department is indicated. The value being from -13 to +13, resuscitation expert consultation is advised.

EFFECT: high evaluation accuracy.

3 tbl

FIELD: medicine, veterinary science.

SUBSTANCE: for the purpose to evaluate the efficiency of tested agents one should detect the content of helminthic eggs in feces of experimental animals before treatment, during treatment and after its ending, and according to early and pronounced increase of eggs' content after the onset of therapy for 1-4 d against initial value and further escape of eggs in feces of treated animals it is possible to detect efficient antihelminthic preparations. The present method enables to apply decreased number of animals in experiments conducted. The method is reliable and quick.

EFFECT: higher efficiency of screening.

4 ex, 5 tbl

FIELD: medicine.

SUBSTANCE: method involves applying integrated diagnostics approach with blood serum examination being applied using infrared spectrometry methods. Sample absorption spectra in the area of 1200-1000 cm-1 are recorded, absorption peak heights having maximum at 1170, 1165, 1140, 1125, 110, 1070, 1025 cm-1 points are measured. Then, mean peak height C and ratio X of peak height at 1125 cm-1 point of maximum to C are calculated. X≥0.7 being the case, light myocardium injury degree is to be diagnosed. 0.7>X≥0.53 being the case, moderate severity degree case is to be diagnosed. X<0.53 indicates grave injury to take place.

EFFECT: high reliability of diagnosis.

FIELD: medicine.

SUBSTANCE: method involves determining blood circulation minute volume from central hemodynamic values by applying ultrasonic examination. Blood circulation minute volume being equal to 4.4-5.6 l/min and higher, moderate severity degree of the process is to be diagnosed. The value being equal to or greater than 5.7 l/min, chronic suppurative process exacerbation is diagnosed. The value being equal to or less than 4.3 l/min, grave chronic suppurative process condition is to be diagnosed.

EFFECT: non-invasive examination process usable in the cases of chronic suppurative processes aggravated with purulent tubo-ovarian neoplasms.

FIELD: medicine, laboratory diagnostics.

SUBSTANCE: the suggested studying should be carried out on the glass simultaneously with several inductors by applying minimal inter-taking antilogarithms concentrations of aggregation inductors which correspond at double combination of inductors: ADP 5.0 x 10-8 M, adrenaline 3.0 x 10-9, collagen - dissolving the main suspension 1:8, thrombin 0.075 U/ml; at triple combination of inductors: ADP 10-9 M, adrenaline 10-9, collagen - dissolving the main suspension 1:9, thrombin 0.060 U/ml. The development of aggregation means thrombocytic activation in patients with arterial hypertension at metabolic syndrome. The method enables to evaluate the changes of thrombocytic functional state with combination of inductors more probably present in area of vascular lesion by applying minimal necessary concentrations that develops real conditions at hemostatic initiation in human vessels.

EFFECT: higher efficiency of studying.

3 dwg, 3 ex, 2 tbl

FIELD: medicine, hepatology.

SUBSTANCE: one should detect splenic length in mm (X1) and circulation in portal vein, moreover, additionally, on should detect volumetric circulation in splenic vein in cu. cm/min. (X2), the index for the ratio of volumetric circulation in splenic vein to the area of longitudinal splenic section (X3), circulatory direction in left-hand gastric vein (X4) by establishing its direction towards the liver to be 1, from the liver to be 2, diameter of splenic artery in cm (X5) and transhepatic portal volumetric circulation in cu. cm/min. (X6), then one should calculate discriminant function Z = 15.9850 - 0.0187X1 + 0.2006X3 - 1.9025X4 - 19.0493X5 - 0.0025X6, where Z - the criterion for predicting "healthy-sick" state; then it is necessary to detect the group with hepatic diseases by the value of Z ≤ 1.621 to calculate for them discriminant function Y = 9.7396 - 0.0279X1 - 0.0018X2 + 0.1873X3 - 4.9174X4, where Y - the criterion to predict "patients with chronic hepatitis - patients with cirrhosis" state and at Y > 1.239 one should diagnose chronic hepatitis, at Y ≤ 1.239 - cirrhosis.

EFFECT: higher efficiency of diagnostics.

3 ex

Up!