Method for early selecting stress-resistant replacement poultry offspring of different genotypes for the purpose of reproduction

FIELD: poultry science.

SUBSTANCE: the present innovation deals with visual evaluation in chicken followed by testing them by the value of bioelectric potential. Chickens with bioelectric potential being significantly higher against average values are considered to be stress-resistant ones and those with bioelectric potential being significantly lower against average values in concrete population are concluded to be stress-sensitive ones. The method is very simple in its implementation and efficient for large-scale selection in poultry on stress-resistance.

EFFECT: higher efficiency.

1 cl, 2 dwg, 2 ex, 4 tbl

 

The invention relates to poultry, in particular to methods of early selection stressoustoicivij repair of the young birds of different directions productivity and crosses for reproduction, taking into account their bioelectric potential (BP).

The known method of selection of birds on stressoustojchivosti during their movement through the narrow horizontal support and the action of the electrical power source is a frequency of 4-7 Hz and energy 2-9 j. Discarded birds that fell from the support (see A.S. USSR №1464993, And 01 To 67/00, 29/00, 1989). The disadvantage of this method is the lack of data on safety, the level of natural resistance and the rate of reaction selected individuals that cannot serve as full proof of their stressoustojchivosti.

The known method of selection stressoustoicivij chickens, where during incubation of the eggs obtained from the evaluated individuals in the period from 1 to 19 day, reduce the temperature within one hour of 37.7 to 27.7°and then determine the viability of embryos and genotype chickens loci, and to stressoustojchivye include chickens, heterozygous for certain loci with viable embryos 60-100% (see A.S. USSR, No. 1436961, And 01 To 67/02, 1986).

The disadvantage is that this method requires highly skilled, specialised laboratories and possible to test only a small poultry.

Closest to the proposed image is the shadow and adopted by the author for the prototype is the method of selection of breeding birds at day old, where conduct a visual assessment of the young phenotypic and form homogeneous groups according to the duration of embryonic development (PEER) within their physiological norm, with an interval of 12 hours (see patent SU # 1419647, And 01 To 67/02, 43/0 30.08.88. Bulletin No. 32).

The disadvantage of this method is that it does not directly take into account the rate of reaction, i.e. a specific way of animal response to changing environmental conditions, depending on the nature of the genotype.

Mass breeding aimed at increasing productivity without taking into account stressoustojchivosti and resistance, may lead to lowering of the preservation and adaptive plasticity birds.

The technical result that can be obtained by carrying out the present invention is to early detection and screening stressoustoicivij, viable young birds for reproduction on the output, or in the period of acquisition stud at 15-16 weeks of age on the magnitude of bioelectric potential, determined after effect of the stresses of the same strength (vaccination, sortirovanie on the floor, marking, transportation to another location), caused, as we assume, Homo - or heterozygous state of the loci responsible for resistance, stressassociated body.

The essence of the invention is achieved in that the air conditioning is haunted chickens after 8-10 h after sampling from the incubator and actions first stressfactor (sexyavenue on the floor, vaccination, tagging) or to feed after carriage in the period of acquisition stud, additional testing on the value of the bioelectric potential (µa µv or any multimeter, for example DT-832 DIGITAL, in the presence of contact plus electrode on the right leg and a minus electrode on the left. To enhance the contact electrodes and the feet are placed in a vessel of dielectric material filled with water or any mesorectum room temperature. To stressoustojchivye include replacements with indicators PSU in µa µv or significantly higher, stresstesting is significantly lower from the average specific populations.

The proposed method was tested on the parent flocks (AB×DM) cross the Anak-Titanium JSC " Podolski", Hisexs White in the SHA "Kuma", SC-Rus-2, MC-123, CJSC "Shpakovskaya" in 2000-2002, (n=7000).

Example 1. Found that when testing chickens of different breeds immediately after sampling from the incubator resolution multimeter DT-832 DIGITAL captures readings only 3-5% of Chicks above 1 µa (exceptAB 1 gr.), therefore, the variability of this characteristic high Cv=228-33%, but after the stress is reduced - Cv=113-20% (table 1).

Stress is the voltage measurement of the state of birds in µv less variabelno: to stress the Cv is 94-1,5%, after stress - 29-0,5%. The coefficients of repeatability µa in populations with wysokim reliable g=0,91-0,71, readings µv is considerably lower and not always reliable, R=0,62±0.19.

The relationship between BP - amperage (mA) and voltage (µv) in animals corresponds to the laws of physics, in our examples, they are everywhere straightforward, positive.

Given that the frequency mkA, MKV and correlation between them is significant (R=0,71±0,13; of-0.62±0,1), consider to determine stressfulness appropriate to use the testimony µa; if not fixed device to take readings µv.

Genetic conditionality of the relationship bioelectric potential with stressfulness and indicators of natural resistance is confirmed by the significantly pronounced difference between maternal and paternal forms on the magnitude of bioelectric potential and change its level following a stress of the same strength (table 1, example 1).

The data show that males AB paternal forms import of the cross "Anak-Titan" after the action of the first stressfactor have indications µa in 55-49 times higher than among male and female maternal forms of diabetes. Males AB are characterized by high uniformity of MCA (Cv=20,0-11,0) and practically do not change the parameters at the first and second definition of BP after stress (1.2 times), that is strasssteine. Paternal form of our

Example 1.

cross SC-Rus-2 to stress has no differences with the parent and only after the stress exceeds 3.9 times µa and 1.6 times µv. Stress responses have expressed increasing µa 28 times and UV - 2.1 times, i.e. stressfully. The parent form (plymouthrock) both crosses response to stress has no differences to increase μa, respectively, 7.9-8.0 and UV - 2.0 and 3.2 times, however, the higher the ICA, the higher mV (voltage).

It is established that BP with age is consolidated and transferred by inheritance. However, more significant correlations father-offspring were observed to stress on the MCA (g=0,35), mcV (R=0,41). Mother-offspring - lower and more pronounced after stress, respectively, g=a 0.27 and 0.30.

Example 2. Both males AB cross "Anak-Titanium"received from pereiras birds were observed lower variability and higher BP=59,2 µa. Both males obtained from young parents, BP below=50,5 MCA and observed significant semannot empirical frequencies from theoretical (Fig 1 and 2). They have been more stressfulness and stronger reacted to the first feed factor (soy milk) increase the level of resistance (1 experimental group, table 2) So, in 3 weeks of age, the increase was: FOREPLAY IS 6.7%, BASK is 4.1%gamma globulin - 4.0% and a decrease in albumin - 2.3% in comparison with the control group.

Phenotypes with a mean BP (II experimental group) were less responsive to increased CARESSES 3.6%, BASK, 2.6%, γ-globulin - 1.7% and a decrease in albumin by 0.8% compared to control.

Changes in phenotypes with high BP (III experimental group) on the forced feeding of soy milk to increase FOREPLAY and BASK were even weaker and therefore amounted to 2.6 and 1.0%, but the increase γ-globulin (3.7%) and decrease in albumin 2.2% were at level 1 of the experimental group, indicating that they have different genetically determined degree of stressoustojchivosti.

Example 2.

Testing of chickens of different genotypes at day old showed the existence of a General principle, the higher the BP, the greater the level of resistance and stressoustojchivosti (example 3, table 3).

So, end of the line AB surpassed cockerels and chickens DM cross “Anak-Titanium” FOREPLAY respectively 8.3 and 10.7%, BASK to 2.0%and 4.6%, total protein - 8.0 and 10.0%. A similar benefit was observed in the level of resistance in cockerels "Laba-5" BP=1.8 µa in comparison with the cockerels BP=0.7 and chickens BP=0.6 μa, CARESSES, respectively, 2.6 and 2.3%; BASK and 3.4 and 5.2%. Both males with low BP through the day after drinking soy milk has noted a significant increase CARESSES 2.7% and BASK - 5.5%are also observed more pronounced the reactions is in individuals with low BP.

The results provided a basis for a conclusion about the opportunity to capture a genetically defined resistance only after pulling Chicks to the influence of the first feed or other factors.

Under other equal conditions of feeding and housing differences in the level of resistance increase or offset depending on stressfulness phenotypes and usefulness of the influence of the first food factor (Patent RU 2179448. The application 13.07.2000. Published 20.02.2002. Bulletin No. 5).

It is established that increasing the level of resistance in the daily age-feeding soy milk contributed to the change in BP between groups. In individuals with low BP indices with age increased, with high - decreased. As a result, at 20 weeks of age in male AB cross "Anak-Titanium noted the same pattern - the higher the BP, the greater the level of resistance. Reliable repeatability of the trait, the presence of correlation with CARESSES, BASK and qualitative composition of the meat (table 4) is proof of the effectiveness of BP for mass breeding of birds for early selection (at the conclusion or at 19-20 weeks of age) vysokorazvynenych, stressoustoicivij Geno - and phenotypes.

Example 3.

Labor costs account for 1 pers./h, h is testing 240 of chickens and the cost of the device from 500-600 rubles Testing can be done at any convenient location.

1. The method of selection of stress-resistant replacements birds of different genotypes for reproduction, including visual assessment of conditioned chickens after sampling from the incubator, or before feeding after transplantation in the period of acquisition of the herd, characterized in that the youngsters are additionally tested for the value of the bioelectric potential (µa µv or) after action process stress-factors of the same strength, however, stress-resistant include replacements with indicators bioelectric potential (µa or µv) significantly higher, and the stress-sensitive include individuals with biological potential was significantly lower from the average specific populations.

2. The method according to claim 1, characterized in that the testing carried out by any multimeter, for example DT-832 DIGITAL, in the presence of plus and minus electrodes respectively in contact with the right and left leg in the vessels of dielectric material filled with water or any mesorectum room temperature.



 

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