Method for preventing afterbirth retention and its degradation

FIELD: veterinary medicine.

SUBSTANCE: method involves subcutaneously introducing immunomodulator like denatured suspended placenta at a dose of 20 ml/head/day 10 days before calving during 10 days.

EFFECT: enhanced effectiveness in preventing fetal envelopes from degradation.

2 cl, 9 dwg

 

The present invention relates to the field of animal husbandry, in particular animal, and can be used in the prevention of infertility in cows due to separation of placenta in physiological terms.

There are ways to prevent the detention of the placenta in cows by application of various biologically active agents: hormonal, enzymatic, anti-bacterial, physical therapy and others[1, 2].

The disadvantages of these methods are: low efficiency due to low resistance of the body, prevent the development of inflammatory processes in placentae, and lack of contractile activity of the uterus.

Also known a method of preventing the arrest of the placenta and postpartum complications in cows [3], which provides for the introduction of tissue beastialiy made from the placenta of cows with specific bacteriophages (prototype).

The disadvantages of this method are: 1) lack of effect of “preservation” of the tissues of the placenta, i.e. the protection and preservation of the structure and function of the tissues of fetal membranes in the delivery period from decay until the moment of separation spontaneously or manually; 2) the lack of an effect of enhancing the overall resistance of the organism of the mother in the prenatal and birth periods;

3) as a consequence, the absence of a stimulatory effect on you is the origin of the fetus;

4) carrying out treatment immediately after birth.

The low resistance of the body of mothers at delivery, causing placental insufficiency and the presence of inflammatory processes in placentae, leads to a rapid decay of tissues of fetal membranes (placenta) immediately after birth. This circumstance does not allow to separate the placenta, for example, RAM (manually) method, because the tissues are torn and exfoliate. In the spring and summer is especially rapid reproduction of microorganisms in the uterus.

The objective of the invention is improving the quality of preventive detention placenta and prevent decomposition of the tissues from the moment of separation by increasing the overall resistance of the organism.

This is achieved by the fact that as a preventive measure applied placenta denatured suspended (MPD) cows or bioglobin [4]. MPD is injected subcutaneously in the neck at a dose of 20 ml/bird/day not more than 10 days before the birth.

MPD (bioglobin - enhanced VCP) - binormality providing immunomodulatory effect due to the presence in their structure of polypeptides, hydroxy acids and nucleic acids.

Example. According to the literature, lack of education in the body of cows prenatal hormones estradiol and cortisol [5], low protein index, and increased with what the actual content of progesterone contribute to the emergence of detention placenta.

In this regard, we selected two groups (#1 and # 3) cows for 10 days before calving by ELISA was noted in the blood, low levels of estradiol and cortisol and group # 2 cows with high levels of estradiol and cortisol. In addition, in the 1st group was initially high levels of progesterone (table 1).

According to these data in groups 1 and 3 predicted retention of placenta, and in group 2 independent of its origin. Cows 3rd group with the aim of preventing the arrest of the placenta was administered subcutaneously in the neck for 10 days before calving MPD dose of 20 ml/bird/day.

Birth of animals took place without complications in physiological terms.

As a result, in the 3rd group after treatment of ELVs the placenta was separated independently in an average of 3.7 hours after childbirth. In the 2nd group, he departed alone in an average of 8.0 hours, but not later than 12 o'clock In the 1st group noted the retention of placenta within 6-7 h after birth and their subsequent fertilization.

In cows the 1st group after calving had elevated levels of estradiol, a prostaglandin that promotes retention of placenta.

In cows 2 and 3 groups at the discharge of the placenta, the amount of estrogen, on the contrary, decreases, which corresponds to the data of the literature.

Thus, initially low levels of estradiol before calving and the subsequent rise after, pursue the corresponding retention of placenta, eliminated prophylactic treatment of ELVs. The dynamics of progesterone is the same in all groups.

The definition of values by the method of the concentration of immunoglobulins in the blood of experimental cows showed (table 2)that in the 1st group is increased by 5.0%. And in the 2nd and 3rd groups, on the contrary, decrease, respectively 19.3 and 9.4%. The increased levels of immunoglobulins is the body's reaction to the development of inflammation in the uterus of cows [6].

In the 2nd group of the inflammatory process was not, as the latter went on their own. In the 3rd group of anti-inflammatory effect of the drug MPD.

Protein ratio in all groups tended to increase after calving: In the 1st group and 0.61-0,72; the 2nd is 0.65 - 0.66 and the 3rd - 0,50-0,54.

Because globulin fractions have the ability to transport steroid hormones, vitamins and other biologically active substances, the best results are marked in the 3rd group, where the number of globulins was the highest - 64,63, in the 2nd - 59.99 and the 1st - 57,83%.

Histomorphological studies of tissue from the placenta of cows experimental groups confirmed the efficacy of PDS.

Paint hematoxylin-eosin. Micmed 2. ×250, 640.

I. retention of placenta

Fetal membranes have significant strukturyzowane, compared with the control group. Was observed almost complete denudation of the terminal villi (Figure 1), places were found remnants of degenerating cytotrophoblast events karyopyknosis and karyorhexis. In the stroma of the villi was observed strongly pronounced capillary hyperplasia with symptoms of hemostasis (Figure 1(1)). Often found where on a smooth chorion remained integrity cytotrophoblast (Figure 2(2)), although the stroma of the villi affected fibonacii, razvlecheniy and swelling. Most of the terminal villi was preserved the integrity of the reservoir cyto - and syncytiotrophoblast. Multinucleated cells were found rarely. In neither case did not meet the hyalinosis of the vessel walls. Were more subtle and smooth, and in the villous chorion destructive and degenerative phenomena, and the capillary network was desolate. Remained syncytiotrophoblast with a specific structure (Figure 3), characteristic for the functioning of the placenta; found numerous syncytial processes and nodes.

II. Self-discharge of the placenta

Fetal membranes had a typical structure. In stem and terminal villi of the chorion basis was presented by connective tissue penetrated the arterial and venous vessels of different caliber (Figure 4(3)). The capillary network was moderately expressed among cell E. the elements of the basis prevailed fibroblastic cells of the row (Figure 5(5) 6(6)), met histiocytes and isolated macrophages type Kashchenko-Hofbauer. Often found lots of razvlechenija basics and edematous effects. The wall of the larger vessels was also with signs of razvlechenija and edema (Figure 4(4)). Often observed phenomenon sclerosis and hyalinosis. The epithelium of the villi places maintained double-layered, showed patches of functionally active syncytiotrophoblast. Cells cytotrophoblast, as a rule, were exposed vacuolar degeneration, picknose and karyorhexis. Some capillaries and venules remained crownpointe. In the decidual tissue was observed collagenase and vacuolization of the intercellular substance (Fig.7, 8(8), 5, 6(7), 9). Terminal villi were covered heavily vacuolation degenerating trophoblasts, among the cells which showed giant cells with OxyFile cytoplasm, sometimes multi-core. Deposits fibrinoid were minimal.

3. Processing VAT.

Fetal membranes cows of this group differed little from fetal membranes cows of the control group, which last went in physiological terms. In large, and often in smaller vessels smooth chorion was observed loosening and swelling of adventitia with the phenomena of hyalinosis, proliferation and obliteration (Figure 10, 11), and razvlechenie and swelling of the stroma of the villi (Fig(10). Among cleto the different elements of the stroma were found mainly fibroblasts, cells Kashchenko-Hofbauer; chorionic epithelium was with the phenomena of degradation and degeneration. Emocapella network was developed moderately. In some capillaries were observed hemostasis (Fig(11)). In networkinfo the chorion also observed hyalinosis of the outer and middle membranes of blood vessels and razvlechenie stroma. In the chorionic epithelium of the villi destructive changes were accompanied by basophilic cytoplasmic granulation, pyknosis and cariolina (Fig), as in the control group. On the terminal villi remained chorionic epithelium with numerous multi-core cells (11, 10(9)), characteristic of the aging of the placenta. Places found villi with fibrinogenami sediments (Fig(12)). Cells cytotrophoblast almost everywhere were with vacuolar degeneration and phenomena of transformation in multinucleated cells (Figure 10). The capillary network of the terminal villi, as in the control group, was moderately cravenplan. The phenomenon of hyalinosis the walls of blood vessels was observed even in the terminal regions of the villi. In the stroma of the villi were identified by razvlechenie, especially near blood vessels and swollen phenomena (Fig, 13).

Thus, from the above description it is seen that at physiological course of pregnancy specific changes in the placenta are one of the factors in normal and timely delivery of the Department the Department of the placenta.

To the prevailing changes in the placenta at the microscopic level, which are signs of its “physiological aging, bringing collagenase stroma of the villi, the phenomenon of destruction and desquamation of the epithelium, as well as fibrinoid deposits around the bare fibers. In addition, there are perivascularly sclerosis and obliteration of openings of different caliber. Fibrinoid fields are often found in okologische space.

During the arrest, in contrast to group self-discharge of the placenta involutive-dystrophic changes in flow poorly. The decidual membrane remains rich in cellular elements. Everywhere there remain signs of maturity of the placenta. Well pronounced vascularization of the villi and sinteticpersona membrane. Often found syncytial knots, typical of normal Mature placenta.

In the stroma of the villi often large, functionally active fibroblasts with a well using processes that are absent in the tissues of the placentas of cows from group self-discharge of the placenta. The stroma of the villi remains in most cases tecophilaeaceae.

Drug use MPD (placenta denatured suspended) contributes to the activation of the functional processes of “aging” of the placenta, also features REGO for change available in placentas of cows in the control group, which creates conditions for physiologically mediated early separation of the placenta.

Sources of information

1. Fedosov NH Prevention detention of the placenta in cows / Nagpadala, Hahanov // veterinary medicine. - 1983. No. 12. - P.48-50.

2. SU 1683759, 5 And 61 To 33/34, 15.10.1991. Method of prevention of postpartum diseases in cows.

3. EN 2149638, 7 AND 61 TO 35/50, 27.05.2000. A method of preventing the arrest of the placenta and postpartum complications in cows.

4. EN 2036651, 6 AND 61 TO 35/50. Biogenic stimulator for the treatment and prevention of diseases of farm animals.

5. Doshukaeva KG hormone levels in the blood of pregnant cows / Cgidev // veterinary medicine. - 1994. No. 5. - P.48-49.

6. Uryupina, NV Microscopic changes in caruncular and cotyledonary in various methods of treatment arrests of placenta / Neverapine, Gagagagaga, Vaastav // veterinary medicine. - 1983. No. 10, - P.45.

A method of preventing the delay of the placenta and prevent its decomposition in cows by introducing immunomodulator, characterized in that as an immunomodulator enter the placenta denatured suspended (MPD) subcutaneously at a dose of 20 ml/bird/day, which begin to enter up to 10 days before calving within 10 days.



 

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