Preparation of sow's sex pheromones

FIELD: veterinary science.

SUBSTANCE: the suggested preparation contains condensate obtained after distillation with urinary vapor of sows being in heat, acetic acid, butyric acid, caproic acid at the following ratio of components, weight%: acetic acid 0.5-2.0, butyric acid 5.0-20.0, caproic acid 0.5-5.0, condensate - the rest up to 100. The preparation is of high biological activity.

EFFECT: higher efficiency.

1 ex, 1 tbl

 

The invention relates to veterinary medicine, in particular the production of biological agents for the stimulation of reproductive function in male farm animals.

Known drug “Stimulen” (Aginary. Intensification play in industrial pig farming. - Abstract. Prof. dis. - Dobrovici, 1995, - 46 C.), which is a mixture of organic substances with attractant (pheromone) activity. However, when using it, as a rule, get low and unstable results.

The closest technical solution, selected as a prototype, is the preparation of the artificial sex pheromone sows (Ed. St. USSR №1720640, And 61 D 19/02 “Artificial sex pheromone sows”, ed. Vphone, Tulyganova)comprising a mixture of organic substances and water in the following ratio of ingredients, wt.%: acetic acid is from 0.2 to 4.3; butyric acid - 4,0-87,0; hexanoic acid - 0,4-8,7; distilled water the rest up to 100.

The disadvantage of this drug is of low biological activity, since it consists of only three components, which are important components of sex pheromones sows, but not the only one. In addition to acetic, butyric and Caproic acids sexual pheromones sows contain other org the organic and inorganic compounds, with attractant activity. This is confirmed by the experimental results: after neutralization of these volatile organic acids in the urine sows she had attractant activity. From 10 sexually Mature boars 7 was positive urine sows without volatile organic acids.

The aim of the invention is to increase the biological activity of the drug.

This objective is achieved in that the drug of sex pheromones contains known volatile organic acid, and optionally enter the condensate obtained after steam distillation of urine sows in sexual hunting, in the following ratio, wt.%: acetic acid is 0.5 to 2.0; butyric acid - 5,0-20,0; hexanoic acid - 0,5-5,0; condensate rest to 100. Condensate urine was obtained using a standard apparatus for steam distillation, which includes a steam generator, a system of tubes for pressure equalization and level measurement water trap Hopkina, running and receiving flask. The process of distillation was carried out by passing steam through the heated urine at a temperature of 95-98°C, followed by cooling of the vapor and condensate (Krekeler, Hackstein. Analytical and preparative laboratory methods. - M.: Chemistry, 1994, - 44-48 C.).

Best cootes which of these components were selected with the use of mathematical planning of experiment method the steep ascent. When you reduce the amount of condensate distillation of urine biological activity of the drug is reduced by 15-25%, while increasing the quantity of condensation activity of the drug is not changed.

Comparative analysis of the prototype shows that after enabling the preparation of a condensate distillation of urine with water vapor biological activity of the drug is greatly increased. Thus, the proposed drug meets the criterion of “novelty”. The study of other well-known technological solutions in this area the features that distinguish the invention from the prototype, were not identified and therefore they provide the proposed technical solution according to the criterion of “significant differences”.

Example. To obtain the drug of sex pheromones of sows in a glass bowl was made of 0.5 ml of acetic acid, 5.0 ml butyric acid, 0,5 Caproic acid was added and condensate distillation of urine to 100 ml of Urine were obtained from sows that were in sexual hunting, using special external urinals. The resulting preparation of sex pheromones was a clear liquid with a specific smell. The drug was Packed up in glass bottles, was checked aluminum caps with rubber stoppers and stored until use at 4-5°C. a shelf Life of 6 months.

Check the RCA experienced a series of drug toxicity confirmed its harmlessness. In the special chamber of the machine with Mature boars using a generator SAG-1 for 60 min was created aerosol air saturation test drug. Behavioral reactions, basic clinical and hematological characteristics of boars in the validation period and the subsequent 7 days was within physiological norms. Was not observed phenomena toxicity and white mice, after intraperitoneal the introduction of the drug in a dose of 0.1 ml/bird all the animals were alive.

Attractant activity of the obtained preparation was determined by the method of biological testing using special olfactometer developed design (Obeid and other Effects of pheromones grunt on the formation of sexual function replacement gilts. Veterinary science. - 1993. No. 3. - P.34-36). The evaluation of the results of biological testing, expressed in percent.

The results showed that the grunts are included in the biological test, percent key (positive) responses to experimental drug was higher (89,6%)than drug-prototype (71,2%).

Production testing of manufactured drug sex pheromones sows were carried out in pig farms Kursk region.

The drug is used for the development of young boars sexual conditional reflex in a stuffed pig. It was formed three groups of 7-nachnyj boars of large white breed (21 goals. in each). The first group of grunts accustomed to captivity after preprocessing stuffed manufactured drug. To do this, the product is evenly applied to the surface of the stuffed by spraying his staff “Rosinka”. Consumption of the drug was 10-15 ml of the Second group of grunts were accustomed to captivity after processing stuffed drug prototype in the same dosage.

The third group of grunts was the control, they were taught to Hutch for a guy that pheromones were not processed.

The results showed (table. 1)that the grunts of the first group sexual conditional reflex was produced faster than males of the second and third group.

Table 1
Effect of sex pheromones sows developing boars reflex on the Scarecrow
GroupnAccustomed to Hutch on the Scarecrow
  boars%
1 (the proposed drug)211571,4
2 (drug-prototype)211152,4
3 (control)21838,1
P is kechnie: teach boars to Hutch on the effigy was carried out for 15 days

Application of the developed drug pheromones sows allows to detect and remove boars with mild sexual reflex and not as “suppliers” of sperm for artificial insemination.

The drug of sex pheromones of sows was introduced in pig farms Kursk region, where artificial insemination of pigs.

The drug of sex pheromones sows containing a mixture of acetic, butyric and Caproic acids, characterized in that the preparation additionally include a condensate obtained after steam distillation of urine sows were in sexual hunting, in the following ratio, wt.%: acetic acid is 0.5 to 2.0; butyric acid - 5,0-20,0; hexanoic acid - 0,5-5,0; condensate - the rest is up to 100.



 

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