Method for increasing resistance to pullorosis in poultry
FIELD: poultry science.
SUBSTANCE: one should irradiate eggs before incubation with the light of He-Ne laser LHN-104, wave length being 632.8 nm, power density at eggs surface being 50 mW/sq.cm/sec, with red light of fluorescent lamp DNESG-500, wave length being 630-650 nm, power at eggs surface being 23.1 erg and with ultraviolet from DRT-400 lamp at wave length being 185-400 nm, average dosage at eggs surface being 20 merg in 3-min-long exposures.
EFFECT: higher resistance to pullorosis in poultry.
4 dwg, 4 tbl
The invention relates to poultry and can be primarily used to increase the resilience of birds to impact exciter pullorosis Salmonella pullorum in a production environment.
The known method of increasing the viability of Chicks by preincubation handling eggs radiation of helium-neon laser AGR-12 a wavelength of 632.8 nm, output power of 15 mW/cm2·C, which stimulates the growth, development, indicators of natural resistance offspring /Petrov E.B. Application rays helium-neon laser for the stimulation of embryogenesis chickens and enhance the livability of Chicks. - Autoreplies...Kida. agricultural Sciences: MVA - 1982 - 20 C./.
There is a method of stimulation of embryonic and postembryonic development of poultry by preincubating irradiation of eggs monochromatic coherent red helium-neon laser LGN-104, a wavelength of 632.8 nm, power on the surface of eggs 50 mW/cm2·with, which lowers embryonic mortality, increased output conforming Chicks on 7,56%, the safety of the birds by the end of the growing - on 5,95% /Mamakaev M.N., Mamakaev A.D. laser irradiation of eggs. Copyright certificate № 1821958 /.
The known method of prevention of pullorosis birds by preincubation handling eggs radiation of helium-neonova the laser LGB-104, a wavelength of 632.8 nm, power density on the surface of eggs 50 mW/cm2·with /Mamakaev mathematical SCIENCES. The decision on the grant of a patent on application No. 2000110969/13/011534, prototype /.
The disadvantages of the known methods is that it does not identify specific sustainability birds to pullorosis in complex preincubation processing of eggs by the emission of a helium-neon laser LGN-104, a gas discharge lamp DMESG-500 and an ultraviolet lamp CES-400, the content and activity of lysozyme, serum birds obtained from irradiated eggs, relative to the test-culture of Micrococcus lisodeicticus and pathogen pullorosis Salmonella pullorum not conducted microbiological testing.
The purpose of the invention is to increase the specific resistance of birds to impact exciter pullorosis Salmonella pullorum in conditions of disadvantaged pricecheapest.
This goal is achieved by the fact that large embryos before incubation irradiated by monochromatic coherent red helium-neon laser LGN-104 a wavelength of 632.8 nm, power density on the surface of eggs 50 mW/ cm2·with red light discharge lamp DMESG-500, a wavelength of 630-650 nm, power on the surface of eggs 23.1 erg and ultraviolet lamp CES-400, wavelength 185-400 nm, the average dose on the surface of eggs 20 mayor/g in the exposures of 3 minutes.
Processing of hatching eggs l is pure energy was performed in a pilot plant for laser light activation /Mamakaev mathematical SCIENCES. Auth. mon. No. 1748768,1989,/.
The content of lysozyme in the serum of broilers of all experimental groups compared to control in all age periods of the studies was higher /table 1/.
|The content of lysozyme in the serum of broilers when laser light activation, n=50 /X±mx/, ug/ml|
|Group||Light sources||The age of birds|
|1 - entry checkpoints for important locations.||-||4,05±0,02||3,35±0,05||2,97±0,05||2,67± 0,08|
|2 experiments||LGN 104||5,93±0,06||4,36±0,06||3,79± 0,02||3,18± 0,05|
|3- '-"||DMESG - 500||to 4.52± 0,05||to 3.92±0,07||3,20±0,04||2,84± 0,06|
|4 - "-″||CES - 400||6,02±0,06||4,36± 0,07||of 3.46±0,02||3,12± 0,05|
|5 - "-"||LGN - 104||6,33±0,04||5,14±0,074,12±0,02||3,88± 0,4|
|DMESG - 500|
|CES - 400|
In day-old broiler 2 group content of lysozyme compared with the 1st group was higher than that of 1.88 mg/ml, 3 by 0.47 μg/ml, 4 - 0.97 µg/ml and 5 - of 2.83 µg/ml In the study of lysozyme in other age periods remains the same pattern with the difference that with age, the birds are decreasing. If Chicks all groups index ranged from 4,05 to 6,33 μg/ml, 15-day - from 3,35 up to 5.14; 30-day - from 2,97 to 4,12 and 45-day - from 2.67 to 3.88 µg/ml And differences of lysozyme between 30 - and 45 - day broilers less contrast than between daily 15 - and 30-day.
The content of lysozyme in the serum of broilers hatched from eggs after laser irradiation /2 g/, was higher than in chickens 3 groups /red-light/, as compared to 5 group /complex processing/ was lower. Differences in the concentration of lysozyme in the serum of broilers 2 and 4 groups /laser and ultraviolet exposure/ were insignificant and were unreliable character / P>0,05 /. Indicators of activity of lysozyme serum shelter is experienced broilers relative to the test culture Micrococus lysodeicticus 0.75 h, the reaction was significantly higher than the results of the control group /31,01% - lysis in 2; 3; 4; 5 groups - 10.68%; 3,12%; 10,96 and 11,98% /2/. Similar data were obtained in studies in 8, 16 and 24 hours of reaction with the difference that differences in the results were more contrast on the 24 hour reaction.
In the study of bactericidal activity of lysozyme serum 30-day-old broilers found that, compared with the first group of poultry 2 groups it was higher by 5.38%, 3 - 1.42%, 4 - 3,20 - and 5-by 7.04%. A similar pattern was also observed in 8, 16 and 24 h of reaction serum and the test microbes, however, better results 4 group relative to the control wore unreliable character /P>0,05/.
The authors estimated the effect activity of blood serum of broilers hatched from eggs after laser exposure was significantly higher than that in the processing of red /P<0,001/. Differences between 1 and 4 groups turned out to be inaccurate /P>0,05/. The activity of lysozyme, serum 5 (integrated treatment) group in all periods of the studies was higher than in poultry group-2 / P<0,001/.
It is established that the activity of lysozyme, serum up to 8 h of reaction was increased, then decreased, reaching the initial level in day-old Chicks for 22-23 h, 30-day - for 18-20 hours This indicates that lysozyme serum up to 8 h of reaction manifests bactericidal action, and from 8 to 16 h - bacter staticheskoe.
Level lysozyme activity of blood serum was higher in broilers 1 - and 15-day-old than in 30-and 45-day that is positively correlated with indicators of lysozyme in the serum.
Analysis of the results of research on the effect of light energy on the activity of the blood serum of experimental broiler chickens showed that laser light treatment in optimal doses significantly contributes to the enhancement of the bactericidal and bacteriostatic activity of blood serum in relation to pathogen pullorosis Salmonella pullorum /1; 2/.
0.75 hour reaction serum daily broilers and Salmonella pullorum regarding the results of the control group % lysis in group 2 was significantly higher on 1,70%3 - 0.44, 4 - 1,95 and 5 to the group - of 2.21%, respectively, after 8 h of reaction - 1,78%; 0,98; 2,67 and 2,79%, after 16 h - 2.05%; or 0.57, 1.64 and 2,72%, after 24 h of reaction - by 1.47%; 0,73; 0,15 and 2.41%, but differences between control and 4 groups were unreliable /P>0,05/.
Thus, the results of the research activity of the blood serum of experimental chickens regarding bactericides and bakteriostatichnosti to Salmonella pullorum show that a steady increase in these parameters were observed in the groups of the laser, red and ultraviolet rays in the complex, as well as the application of laser beams. In the group of ultraviolet light after 1 h incubation ingredients there has been a rapid decline of serum, reaching the initial level of optical density.
Research is seen a natural increase in the activity of lysozyme to 8 h of incubation and decreased results after 8 h, reaching minimum values, except for the integrated exposure of the eggs.
It is established that the activity of blood serum monthly broilers in all experimental groups was higher than in control. The exception is group 3, where the difference with the control for 16 h research was minimal.
When considering optical density of some samples after incubation of serum monthly broilers and pathogen pullorosis Salmonella pullorum in solution was observed a sharp decrease in the optical density and the emergence of a flocculent precipitate, which indicates that the agglutination reaction.
Generalizing the results of the research, it should be noted that the determination of bactericidal and bacteriostatic activity of blood serum can be recommended as a test to determine the resistance of birds pullorosis-typhoid. It indicates a higher degree of resistance of the organism birds obtained from eggs after light treatment before laying to incubation. The best results were registered in the group of calves derived from eggs after complex formation is otci laser, red and UV light. When comparing the use of the laser light, and ultraviolet light for up to 16 hours of incubation significantly different results were reported, but after 16 hours under application of the UV activity of the blood serum decreases more sharply in day-old Chicks, reaching control values. Regarding the group's use of monochromatic red light of the laser light more active in the stimulation of bactericidal and bacteriostatically properties of serum.
Research results lysozyme, bactericidal and bacteriostatic activity of the blood serum of broilers when laser light activity positively correlate with viability of birds in postnatal ontogenesis /table 3/.
|The safety of broilers when laser light activation, n=200, /X±mx/|
|Group||Light sources||The age of the birds, days|
|1 - counter.||-||194,19±0,89||97,10||186,27± 2,18||93,14||180,18±1,84||90,09|
|2 - experienced.||LGN-104||198,49±0,80||99,25||l95,97±1,13||97,99||190,82±1,40||95,41|
|3 experienced.||DMESG-500||199,77±1,23||99.89 per||192,64±1,13||96,32||187,33±1,90||93,67|
|4 ~ experienced.||CES-400||199,16±0,33||99.58||195,9± 1,16||97,96||190,40±1,56||95,20|
|5 - experienced.||LGN-104 DMESG-500 DPT-400||199,99±0,09||99,99||198,45±1,36||99,23||192,27±1,80||96.14|
In broilers, the laser light output by the processing of hatching eggs, no adverse events were observed.
It is established that at 15 days of age the safety of broilers compared with the control was higher in group 2 on 2,21%; 3 - 2,87%; 4 - 2.56% and 5 group by 2.99%. Similar results registered by 30 day. 45 day screening compared with the control, the safety of broilers was higher in group 2 - 5,91%, 3 - on of 3.97%, 4 - 5,67% and 5 to the group - of 6.71% /P<0,01-0,001/.
The safety of the birds was higher in the group of broilers obtained from eggs after complex processing laser, red and UV light. Significant differences in the viability of the birds after the laser and ultraviolet irradiation was not detected.
The overall incidence of broilers to 30 days of age was in the control group -15,5%; the irradiation of laser light is 16.5%, red and UV light by 16.0%, and when integrated impact - 15%. Overall mortality was respectively 38,71%, 21,21%; 28,12%; 25,00%, 16,67%/table. 4, 3, 4/.
|The monthly incidence of broilers pullorosis when laser light activation and mortality, n=200.|
|Group||The light source||Livestock the age of one month, a goal||Ill. goal||Palo total, goal||Selected pathogen pullorosis, sample||Reacted positively RA sample||Total ill-pullorosis goal.|
|1 - counter.||~||188||31||12||6||24||30|
|5-experienced.||LGN-104 DNEST-500 DPT-400||195||30||5||2||32||34|
Bacteriological study of poultry waste to the age of one month of cultivation showed that in the control group of 12 investigated the fallen chickens selected pullorosis Salmonella pullorum, respectively, when exposed to laser LGN-104 - 7 and 4, the lamp DNEST-500 - 9 and 5, the Jack Russell-400 - 8 and 5, and when integrated impact - 5 and 2.
Serological studies on agglutination reactions respond positively in group 1 of 188 samples 24 /12,77%/2 of 193 29 /15,03%/, 3 from 191 26 /13,61%/4 - 28 of 192 /14,58/ and 5 group of 195 32 /16,41%/.
Therefore, according to bacteriological, serological studies of 200 broilers in the experience of contact with the causative agent of pullorosis in group 1 and 15%; in 2 and 4 - 16.5%; 3 - 15,5% and 5 group - 17%. Mortality was 20,00; 12,12; 16,13; br15.15 and 5.88 per cent in the respective groups.
Thus, the results of the research total isresponse, the survival rate of birds in conditions unfavorable for pullorosis economy, bactericidal and bacteriostatic activity of lysozyme serum relative to the test microbes Micrococcus lisodeicticus, in which Sudetes of pullorosis Salmonella pullorum serological and microbiological studies show the use of the proposed method preincubation comprehensive treatment of chicken embryos by laser radiation of LGN-104, lamps DMESG-500 and he-400 exposures of 3 minutes increases the resistance of birds to impact exciter pullorosis-typhoid birds.
The method of increasing the resistance of birds to pullorosis, including preincubation handling of eggs by the emission of a helium - neon laser LGN-104, a wavelength of 632.8 nm, power density on the surface of eggs 50 mW/cm2·, characterized in that preincubation processing of eggs was performed comprehensively light helium-neon laser LGN-104, a wavelength of 632.8 nm, power density on the surface of eggs 50 mW/cm2·with gas discharge lamp DMESG-500, a wavelength of 630-650 nm, power on the surface of eggs 23.1 erg and mercury-quartz lamp CES-400, wavelength 185-400 nm, power on the surface of eggs 20 mayor/g, exposures of 3 minutes.
FIELD: biotechnology, vaccines.
SUBSTANCE: vaccine comprises bacterial mass of Pasteurella multocida of serovariants A, B and D, Haemophilus pleuropneumonia of serogroups 1 and 2, and streptococcus of serogroups C and R, and also lysate-anigens of salmonellae Salmonella cholerae - suis, strain № 370 and Salmonella typhimurium № 415 mixed in the definite concentration. Vaccine elicits the high immunogenicity and provides the protection of pigs against infectious pneumonia of bacterial etiology and salmonellosis.
EFFECT: valuable veterinary properties of vaccine.
3 cl, 1 tbl, 5 ex