Method for preparing erythrocytic mass to be modified with antibiotics

FIELD: medicine.

SUBSTANCE: method involves exposing erythrocytic mass, produced in apparatus, to ultraviolet radiation of wavelength equal to 256 nm produced with a lamp source operating during 15-20 min. Then it is modified with an antibiotic and returned to patient.

EFFECT: enhanced effectiveness of treatment; increased sensitivity to antibiotics; reduced drug consumption.

 

The present invention relates to medicine, namely Transfusiology for preparation of erythrocyte mass for subsequent modification by her antibiotics for the treatment of inflammatory diseases (group delay).

In the treatment group delay is the most important place is occupied by antibacterial drugs. However, often when group delay their reduced bioavailability due to the clear deterioration of the microcirculation in inflammation, which leads to reduced clinical efficacy of antibiotics.

Therefore, the success of antibiotic therapy depends not only on the correct choice of drug to which sensitive causative agent of the disease, but from the way his "delivery" to the site of infection (Lasswell, Lasalyano, Kguseynov, Nagsimula, Lshkurvn. Features UV treatment autologous blood patients to optimize antibiotic therapy. Materials of the III Congress of photobiologic Russia, Voronezh, 2001, s).

In the treatment group delay recently use the methods in medicine, including ultraviolet irradiation of autologous blood and in vitro modification of erythrocyte mass antibiotic.

Closest to the proposed invention is a method, when after drawing blood from a patient in the plasmapheresis procedure was performed in vitro modification of erythrocytes antibiotics with subsequent is relevant to their introduction into the patient's body (Gampopa, Savrasov, Vmmap. Reinfuse cell mass of the blood after incubation with the antibiotic in the treatment of uncomplicated pneumonia in children. Efferent therapy. 1998, Vol. 4. No. 4. P.47-50). However, the methodology did not include pre-ultraviolet irradiation of the erythrocyte mass.

The use of ultraviolet irradiation of red blood cell mass in the process of its preparation for further in vitro modification of the antibiotic in the blood is not known.

The technical object of the present invention is to provide a method of preparation of the erythrocyte mass before the subsequent modification of it with antibiotics to increase the bioavailability of antibacterial drugs.

The problem is solved by carrying out plasmapheresis emitting erythrocyte mass, with the resulting erythrocyte mass in the "Source" is exposed to ultrafiolyetovykh rays with a wavelength of 256 nm (source lamp [DRB]-8) for 15-20 minutes with the subsequent modification of the antibiotic, and return of the patient.

The method is as follows.

Under aseptic conditions from the cubital vein of a patient using a plastic container Gamecon" 500/300 ml produce fence 400 ml of whole blood, with the aim of substitution patient is injected with 0.9% sodium chloride solution 300 ml drip.

"Gamecon" centrifuged in a centrifuge RS-6 mode 2900 rpm for 10 minutes, then from the container using plasmacytoma remove the plasma. Erythrocyte mass, obtained alertnum by apparatus "Source" is exposed to ultraviolet rays with a wavelength of 256 nm for 15-20 minutes; the ultraviolet source lamp is [DRB]-8. Then erythrocyte mass modify antibiotic and is re-infused to the patient.

The effectiveness of the proposed method is investigated experimentally. Estimated measure of the sensitivity of microorganisms to ampicillin, the inhibitory growth of the pathogen in three series (4-I control), with different ways of administration of the drug.

Criteria of sensitivity was determined by the conventional 4-point scale microbiological studies (Order No. 250 the USSR Ministry of health).

Pre reliably determined susceptible to this antibiotic. The method of diffusion in agar was carried out by paper disks with further incubation at 37 ° °and the final stage is the definition of the zone of growth inhibition of bacteria, which characterizes the degree of susceptibility or resistance to the drug.

In the experiment, 1 ml of bacterial suspension was made on sterile nutrient agar in Petri dishes. Then, on the surface of the medium were applied to paper disks, etc the supply of various investigated the composition. The first series - canned by the blood of the patient with an antibiotic, second series - erythrocyte mass, obtained from blood of a patient with an antibiotic, third series - proceeded treated with ultraviolet light according to the described method and antibiotic and control series - only antibiotic. In each series of 10 samples.

The results showed that the zone of growth inhibition of microorganisms in the 3rd series was maximum (25±2 mm), which significantly exceeded the target by 19%, and the 1st and 2nd series, 4 and 5%, respectively (p less than 0.05).

Thus, the positive effect of the proposed method is to increase the sensitivity of microorganisms to antibiotics, which is associated with a combination of membranotropic effects of ultraviolet irradiation of blood and in vitro modification of the formed elements of the blood with the use of antibiotics, which gave the ability to potentiate the pharmacological effect of drugs.

The proposed method allows to increase the bioavailability of antibacterial drugs in terms of group delay and reduce the amount of drugs that optimizes antibacterial therapy.

The method of preparation of erythrocyte mass for subsequent modification of its antibiotics, including plasmapheresis with the release of erythrocyte mass, characterized in that the obtained eritr Citaro mass in the “Source” is exposed to ultraviolet rays with a wavelength of 256 nm (source - lamp [DRB]-8) for 15-20 min, with subsequent modification of its antibiotic and return to the patient.



 

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