Strain propionibacterium freudenreichii subspecies shermanii as producer of fodder protein

FIELD: biotechnology, food industry, agriculture, microbiology.

SUBSTANCE: the strain Propionibacterium freudenreichii subsp. shermanii Ac-103/12 is obtained by multiple re-inoculations of the parent strain Propionibacterium freudenreichii subsp. shermanii Ac-103 cells and deposited in All-Russian State collection of microorganism strains used in veterinary science and animal husbandry at number VGNKI - 03. 04. 12. - DEP. This strain is a producer of fodder protein that allows excluding the environment pollution in manufacturing the protein fodder, to enhance the specific yield of protein, to reduce energy consumptions in manufacturing protein fodder, to simplify and to accelerate the process for its preparing, to simplify the equipment, to utilize wastes in processes using the natural raw.

EFFECT: valuable properties of strain.

2 tbl, 10 ex

 

The invention relates to food industry, biotechnology, agriculture.

Known yeast Saccharomices cerevisiae race-1986 - (Patent RU №2183666, 12 F 3/10, publ. 2002) [1].

Yeast this race is used for digestion of the grain mash in alcohol production. Their function is the processing of sugars in the wort into alcohol. The feed product is not the target product, its utilitarian function is not defined. He is the alcohol production waste, polluting the environment. Control over the quality and composition of the waste difficult to implement.

A known strain of the yeast Candida tropicalis BWA-C producing fodder protein (Patent RU №2042713, 12 P 1/100, publ. 20.06.2002,) [2] (the prototype).

The disadvantage of this known strain is use for feed protein of the yeast Candida, which are conditionally pathogenic microorganism that requires additional process of thermolysis and special equipment, a complex system of neutralization of effluents and air emissions from large volumes of used air. Sanitary and epidemiological rules SanPiN 2.3.2.1078-01 yeasts of the genus Candida included in the list of substances that have harmful effects on human health. The growth rate of cells known strain is low, which contributes to the production of the n cycle and reduces the specific yield of the target product at the stage of biosynthesis. In addition, in the preparation of feed protein by the use of this famous strain of yeast that requires a large consumption of heat and energy for drying biomass and considerable expense (1.5 g/l) ammonium sulfate.

The technical result achieved by the present invention, is the elimination of environmental pollution, the reduction of energy consumption in the preparation of feed protein, simplification of apparatus and equipment, disposal of waste products, using natural raw materials, increasing the biological value of feed protein by providing opportunities to enrich the intestinal microflora of animals living cells propionic acid bacteria.

This technical result is achieved by the fact that, as a producer of protein feed use newly selected strain of Propionibacterium freudenreichii subsp. shermanii 103/12 deposited in the all-Russian State collection of strains of microorganisms used in veterinary medicine and animal husbandry, the registration number of a strain of Propionibacterium freudenreichii subsp. shermanii VGNKI-03.04.12.-DEPT (123022, Moscow, highway, and 5). The location of the strain collection is defined microorganisms wildebeest VNIIBT (111033, Moscow, street powered wheelchairs and scooters, dB).

The use of bacteria as a producer of protein feed is more effective because the bacteria produce up to 75% of the be is the spacecraft mass, while the yeast is not more than 60%. The use of a new strain of Propionibacterium freudenreichii subsp. shermanii 103/12 for the preparation of protein meal does not require air consumption and energy consumption per presentation, because this strain of propionic acid bacteria is gone anaerobic. The strain has a broad spectrum antimicrobial action, which eliminates the development of foreign microflora in the process of biosynthesis, and therefore does not require special equipment for compliance with conditions of sterility. Opportunities for the recycling of various waste industries using natural raw materials, while increasing the biomass of a new strain with the purpose of preparation of protein feed solves environmental problems of enterprises.

Below are examples of implementation of the invention.

Example 1.

The strain of Propionibacterium freudenreichii subsp. shermanii 103/12 was obtained by multiple (at least 30) of the re-seeding of the cells of the original strain of Propionibacterium freudenreichii subsp. shermanii Ac-103 on a dense nutrient medium of the following composition:

Distilled water 1000 ml.

Yeast extract 10.0 g,

KN2PO41,0,

Na2HPO4·2H2O 3,0,

Sodium lactate (70% solution) 40,0 ml

CoSO41,0 mg

Agar 20,0,

The content of free propionic acid in the medium was gradually increased from 0 to 1.5%. Cells were incubated on nutrient dense is the ed at 37° C for 24 hours. The selection was carried out based on the number of colonies and acid-forming capacity, which was determined by titration. Was selected those colonies growing on solid culture medium is not inhibited in the presence of 1.5% propionic acid. After 30 transfers the strain reached genetic stability. The results of the selection of a new strain of Propionibacterium freudenreichii subsp. shermanii 103/12 by step breeding are shown in table 1, which shows that when the content in the environment of propionic acid to 1.0% of the cell growth of a new strain of propionic acid bacteria (unlike the original strain) is not inhibited and is characterized by a sufficiently large number of cells. When larger, the content of propionic acid (1,5%) there is a sharp decrease in the number of cells, and the morphology of the cells changed.

For breeding strain selected the largest colonies were perseval on liquid nutrient medium of the following composition:

Distilled water 1000 ml.

Yeast extract 10.0 g,

KH2PO41,0,

Na2HPO4·2H2O 3,0,

CoSO41,0 mg

Sodium lactate (70% solution) 40,0 ml

For long-term storage of cells of strain lyophilizer in separated milk and store in the absence of oxygen. Cells of strain can also XP is a thread on the 10%sacharose-gelatin agar or in semi-solid medium under oil.

The strain is not zoopathogenic or phytopathogenic. It is not dangerous for other reasons.

In accordance with Bergey′ s Manual of Determinative Bacteriology, 9th edition, 1994, Williams &αμπ; Wilkins, USA selected strain identified as Propionibacterium freudenreichii subsp. shermanii 103/12.

Culture-morfologicheski particular strain still sticks by 0.5-1.5 mm, sometimes forming short curved chains, sometimes have the form of cocci. Gram-positive. Nesporoobrazuth. Aerotolerance. Sprayway fructose, lactose, galactose, glucose, mannose. The main product of metabolism - propionic acid. In the cell wall of the main component is meso-DAN. The optimum pH of 7.0 to 7.2. The optimum temperature 32° C.

Example 2.

The strain of Propionibacterium freudenreichii subsp. shermanii 103/12 was assessed by the ability to form a high content of protein by culturing it on a liquid nutrient medium composition specified in example 1, the results of measuring the amount formed in the culture fluid of biomass and crude protein.

The inoculate in the amount of five volume percent was introduced into the flask with the liquid nutrient medium with a volume of 750 ml and were cultivated in a hospital if the following parameters: temperature 37° C, pH 6.0. As the neutralizing agent used a 20%solution of g is kookie sodium. After 24 hours of cultivation was determined by the growth of bacteria and the number formed during the biosynthesis of biomass and crude protein in the culture fluid.

The results obtained are presented in table 2.

Table 2

Characterization of selected strains of Propionibacterium freudenreichii subsp. shermanii 103/12
Name of indicatorValue
The number of cells in 1 ml culture fluid0,7· 109
The specific output from the substrate, wt. %95,0
Mass fraction of crude protein, %47

On the basis of obtained results it is concluded that the selected strain has a high productivity in relation to the target product - accumulation of biomass (initial content of bacterial cells was equal to 1.0· 102), high specific output from the used substrate and the accumulation of crude protein to 47% vs. 25% of the original strain. The strain can be used on an industrial scale.

A new strain of Propionibacterium freudenreichii subsp. shermanii 103/12 allows to obtain protein food with high output when disposing of waste products: distillery stillage, spent grains, waste flour and to omalinova productions waste grain and fruit raw materials.

Example 3.

In distillery vinasse contribute 1.1 mg/l CoSO4and grown pure culture of Propionibacterium freudenreichii subsp. shermanii 103/12.

At the end of the fermentation process the resulting mass is divided into solid and liquid phase by filtration.

The solid phase is sent to drying at a temperature (80-90)° C.

Feedstuff obtained after drying of the solid phase contains 45.9% of crude protein and has the following composition:

mass fraction of carbohydrates, % SV 44,2

mass fraction of ash, % 2,5 SV

the total amino acid content, % 43,4

of them

lysine + histidine 2,15

arginine 1,25

aspartic acid 4,25

threonine 2,0

serine 2,08

glutamic acid 14,84

methionine + cystine 1,3

glycine 2,6

Proline 3,14

phenylalanine + tyrosine 2,65

alanine 4,4

isoleucine + leucine 2,53

mass fraction of protein Burstein, % SV 37,1

mass fraction of fat,% SV 5,1

the total content of trace elements, mg/kg 21800

of them

phosphorus 5900

potassium 3500

sodium 500

calcium 11600

magnesium 1000

iron 750

cobalt 4,9

the vitamin content, mg/kg

E (tocopherol) 43,5

B1(thiamine) 3,3

In2(Riboflavin) 7,7

In3(Pantothenic acid) 35,3

In4(choline) 700

In5(nicotinic acid) 26,8

In6(pyrid the Xin) 8,2

In9(folic acid) 18,0

In12(ciankobalamin) 34,4

the exchange energy, MJ 13,4

fodder units, kg 1,37

Obtained after filtering the liquid phase content in g/l:

dry matter 10,1

mass fraction of nitrogenous substances 1,04

mass fraction of ash 1,3

mass fraction of fat 0,006

mass fraction of carbohydrates 1,7

mass fraction of ammonia nitrogen 0,04

the total amino acid content,% 0,29

the total content of trace elements 1,05

the total content of vitamins 1,01

the content of lactate (propionate), g/100 ml of 1.12

Received feed product contains living cells propionic acid bacteria, enriching the intestinal microflora of animals consuming food that increases its biological value.

Example 4.

Culture liquid after fermentation with Propionibacterium freudenreichii subsp. shermanii 103/12 in example 3 is subjected to pre-filtering. After filtration of the precipitate obtained by the humidity of 50-60%, which can be used as a ready wet food.

Example 5.

In milk serum solids content of 4-5% add 0.9 mg/l CoCl2is heated to 50° To contribute 20% vol. cells of a pure culture of Propionibacterium freudenreichii subsp. shermanii 103/12. The incubation is conducted for 3 hours at a temperature of 45° and a pH of 5.9 to 6.0. After drying the obtained biomass gain b is lkove-vitamin food containing living cells propionic acid bacteria in the exponential growth phase.

Example 6.

Brewer diluted with tap water at a ratio of 1:1, set the pH at 5.8 to 6.0, temperature 59-61° and contribute to 1.4 u/g conditional starch α -amylase. The mixture is kept under these conditions for 55 min, and then 55 minutes at a temperature of 74-76° C. After cooling the mixture to 59-61° give it to 0.4 u/g α -amylase and maintained under these conditions for 35 minutes the Mixture is cooled to 45-50° and contribute to 5.5 units/g conditional starch glucoamylase incubated at pH 5.0 to achieve the content of reducing substances 6%, then cooked fermentolizat spent grains impose 1.1 mg/l CoSO4and pure culture of Propionibacterium freudenreichii subsp. shermanii 103/12 in an amount of 5 vol.%. Incubation is carried out at periodic stirring for 20 hours at a temperature of 50° and a pH of 5.9 to 6.0.

The resulting biomass of propionic acid bacteria in the exponential growth phase, dried and get protein and vitamin food.

Cell viability in the finished dried protein stern preserved.

The obtained protein-vitamin product contained protein 49.6% SV, amino acids to 44.1% for SV, carbohydrates 37,7% SV

Example 7.

Milling waste is crushed to pass at least 80% through a sieve with cells of diameter is ω 1 mm Prepare aqueous suspension of powdered milling waste by mixing them with water in the ratio 1:3. In the prepared suspension is made of 20 u/g of dry matter of the pulp cellulolyticus enzyme β -glucanase and incubated the mixture at a temperature of (59-61)° C and pH 6.0 for 55 min, and then at a temperature of 105° C for 25 minutes to reach the content of reducing substances 3-5%, then also add to 8 IU/g cellulose β -glucanase and 5 u/g conditional starch glucoamylase. The mixture is maintained at a temperature of 45° C and pH 5.0 for 30 min before reaching the content of reducing substances 7-10%.

After processing in osaharennoe mass contribute 0.9 mg/l CoCl2and implement it on the incubation of microorganisms Propionibacterium freudenreichii subsp. shermanii 103/12 at a temperature of 45° and a pH of 5.9 to 6.0. After 24 hours, the formed protein-vitamin product with a mass fraction of protein to 49.6% in SV, with the amino acid content of 44.1% for SV, the carbohydrate content of 37.7% SV

Example 8.

Fruit pomace with a humidity of 50-60% is diluted with water in the ratio 1:2, the mixture is heated to a temperature of 45-50° With, in it add the cellulase in the amount of 1.8 u/l and the mixture was incubated for 3 h at 50°C until the content of reducing substances 10-15% with obtaining fermentolizate. Add Aut 0.9 mg/l CoCl 2and seeded with a pure culture of microorganisms Propionibacterium freudenreichii subsp. shermanii 103/12, which contribute to the nutrient medium in the amount of 20% by volume environment. Incubation is carried out at periodic stirring at a temperature of 40° and a pH of 5.9 to 6.1 in 48 hours.

Example 9.

Starch waste is treated similarly raw grain and milling waste, excluding stage of grinding. When the preparation of a mixture of starch waste water the amount of water is adjusted to obtain a slurry concentration of 14-16%.

On the environment carry out incubation Propionibacterium freudenreichii subsp. shermanii 103/12.

Get food, rich in biologically active substances and protein.

Example 10.

The crushed grain, milling waste or starch waste is used to make fermentolizate by treating them with aqueous suspensions of amylolytic enzymes. To do this, in aqueous suspension contribute to 1.4 u/g conditional starch α -amylase, and the mixture is kept for 55 min at a temperature of 59° and a pH of 5.8, then 35 min at 95° C, after which the mixture is cooled to 45° With, give it to 0.4 u/g conditional starch α -amylase and 6.5 u/g conditional starch glucoamylase. Stand the mixture for 30 min at a temperature of 45° C and pH 5.0 to achieve the content of reducing substances 7% with obtaining fermentolizate where Inc is berout pure culture of Propionibacterium freudenreichii subsp. shermanii 103/12 with obtaining adequate protein and vitamin food.

The strain of Propionibacterium freudenreichii subsp. shermanii VGNKI-03.04.12.-DEPT producing fodder protein.



 

Same patents:

FIELD: biotechnology, medicine, microbiology, agriculture, food industry.

SUBSTANCE: the strain Lactobacillus plantarum P4 deposited in the collection of NIIM MO RF at number 39 and the strain Lactobacillus buchneri P0 deposited in the collection NIIM MO RF at number 38 form in common a symbiotic system characterizing by syntrophic synergistic type of relationship. The probiotic preparation "Bilakt" based on thereof comprises the dried mixture of strains Lactobacillus plantarum P4 and Lactobacillus buchneri P0 in the ratio of microorganisms from 4:1 to 1:4 and products of their vital activity. This mixture promotes to sanitation of nonsterile cavities of body and correction of dysbacteriosis of different etiology in sick humans and animals at the content of live cells 1 x 108/1 ml of rehydrated preparation, not less. The preparation shows high effectiveness in some human diseases - digestive and urogenital tracts, different cutaneous diseases of different etiology (suppurative and burn injuries of skin) and agricultural animals and poultries.

EFFECT: valuable medicinal properties of strain and probiotic.

4 cl, 3 ex

FIELD: biotechnology, medicine, microbiology, agriculture, food industry.

SUBSTANCE: the strain Lactobacillus plantarum P4 deposited in the collection of NIIM MO RF at number 39 and the strain Lactobacillus buchneri P0 deposited in the collection NIIM MO RF at number 38 form in common a symbiotic system characterizing by syntrophic synergistic type of relationship. The probiotic preparation "Bilakt" based on thereof comprises the dried mixture of strains Lactobacillus plantarum P4 and Lactobacillus buchneri P0 in the ratio of microorganisms from 4:1 to 1:4 and products of their vital activity. This mixture promotes to sanitation of nonsterile cavities of body and correction of dysbacteriosis of different etiology in sick humans and animals at the content of live cells 1 x 108/1 ml of rehydrated preparation, not less. The preparation shows high effectiveness in some human diseases - digestive and urogenital tracts, different cutaneous diseases of different etiology (suppurative and burn injuries of skin) and agricultural animals and poultries.

EFFECT: valuable medicinal properties of strain and probiotic.

4 cl, 3 ex

FIELD: biotechnology, microbiology.

SUBSTANCE: the strain Lactobacillus paracasei CNCM I-2116 (Ncc 2461) is able to attach with mammal intestine mucosa and to grow in the presence of up to 0.4% of bile acid salts and to prevent the infection of intestine epithelial cells with rotaviruses. Agent shows the content from 1 x 105 to 1 x 1012 CFU of the strain L. paracasei I-2116 (NCC 2461) and can be used for preparing an agent and foodstuff taken among milk, yogurt, cheese, dry milk, children's nutrition products or a pharmaceutical preparation taken among a liquid bacterial suspension, dry oral supplement, liquid oral supplement, dry product for feeding through a tube or liquid product for feeding through a tube. Invention provides the enhanced viability of the strain in its using and effectiveness in prevention of adhesion to intestine cells and invasion of pathogenic microorganism cells in intestine causing diarrhea. Invention can be used in food industry and medicine in prophylaxis and/or treatment of diarrhea-associated diseases.

EFFECT: valuable medicinal properties of strain and agent.

3 cl, 5 dwg, 7 ex

FIELD: biotechnology, microbiology.

SUBSTANCE: invention proposes a method for preparing exopolysaccharide by culturing microorganisms in nutrient medium containing one or more carbon source assimilated by microorganisms and caruba seeds fraction as nitrogen organic source. Applying the proposed method provides preparing exopolysaccharide eliciting improved organoleptic, sensor and visual properties. Invention can be used in building, paper, textile, cosmetic, food, oil output industry and agriculture.

EFFECT: improved preparing method.

15 cl, 4 ex

FIELD: biotechnology, microbiology.

SUBSTANCE: invention proposes a method for preparing exopolysaccharide by culturing microorganisms in nutrient medium containing one or more carbon source assimilated by microorganisms and caruba seeds fraction as nitrogen organic source. Applying the proposed method provides preparing exopolysaccharide eliciting improved organoleptic, sensor and visual properties. Invention can be used in building, paper, textile, cosmetic, food, oil output industry and agriculture.

EFFECT: improved preparing method.

15 cl, 4 ex

FIELD: biotechnology, microbiology.

SUBSTANCE: invention proposes a method for preparing exopolysaccharide by culturing microorganisms in nutrient medium containing one or more carbon source assimilated by microorganisms and caruba seeds fraction as nitrogen organic source. Applying the proposed method provides preparing exopolysaccharide eliciting improved organoleptic, sensor and visual properties. Invention can be used in building, paper, textile, cosmetic, food, oil output industry and agriculture.

EFFECT: improved preparing method.

15 cl, 4 ex

FIELD: biotechnology, microbiology.

SUBSTANCE: invention proposes a method for preparing exopolysaccharide by culturing microorganisms in nutrient medium containing one or more carbon source assimilated by microorganisms and caruba seeds fraction as nitrogen organic source. Applying the proposed method provides preparing exopolysaccharide eliciting improved organoleptic, sensor and visual properties. Invention can be used in building, paper, textile, cosmetic, food, oil output industry and agriculture.

EFFECT: improved preparing method.

15 cl, 4 ex

FIELD: biotechnology, microbiology.

SUBSTANCE: invention proposes a method for preparing exopolysaccharide by culturing microorganisms in nutrient medium containing one or more carbon source assimilated by microorganisms and caruba seeds fraction as nitrogen organic source. Applying the proposed method provides preparing exopolysaccharide eliciting improved organoleptic, sensor and visual properties. Invention can be used in building, paper, textile, cosmetic, food, oil output industry and agriculture.

EFFECT: improved preparing method.

15 cl, 4 ex

FIELD: biotechnology, microbiology.

SUBSTANCE: invention proposes a method for preparing exopolysaccharide by culturing microorganisms in nutrient medium containing one or more carbon source assimilated by microorganisms and caruba seeds fraction as nitrogen organic source. Applying the proposed method provides preparing exopolysaccharide eliciting improved organoleptic, sensor and visual properties. Invention can be used in building, paper, textile, cosmetic, food, oil output industry and agriculture.

EFFECT: improved preparing method.

15 cl, 4 ex

FIELD: biotechnology, microbiology.

SUBSTANCE: invention proposes a method for preparing exopolysaccharide by culturing microorganisms in nutrient medium containing one or more carbon source assimilated by microorganisms and caruba seeds fraction as nitrogen organic source. Applying the proposed method provides preparing exopolysaccharide eliciting improved organoleptic, sensor and visual properties. Invention can be used in building, paper, textile, cosmetic, food, oil output industry and agriculture.

EFFECT: improved preparing method.

15 cl, 4 ex

FIELD: biotechnology, medicine, microbiology, agriculture, food industry.

SUBSTANCE: the strain Lactobacillus plantarum P4 deposited in the collection of NIIM MO RF at number 39 and the strain Lactobacillus buchneri P0 deposited in the collection NIIM MO RF at number 38 form in common a symbiotic system characterizing by syntrophic synergistic type of relationship. The probiotic preparation "Bilakt" based on thereof comprises the dried mixture of strains Lactobacillus plantarum P4 and Lactobacillus buchneri P0 in the ratio of microorganisms from 4:1 to 1:4 and products of their vital activity. This mixture promotes to sanitation of nonsterile cavities of body and correction of dysbacteriosis of different etiology in sick humans and animals at the content of live cells 1 x 108/1 ml of rehydrated preparation, not less. The preparation shows high effectiveness in some human diseases - digestive and urogenital tracts, different cutaneous diseases of different etiology (suppurative and burn injuries of skin) and agricultural animals and poultries.

EFFECT: valuable medicinal properties of strain and probiotic.

4 cl, 3 ex

FIELD: biotechnology, microbiology.

SUBSTANCE: the strain Lactobacillus paracasei CNCM I-2116 (Ncc 2461) is able to attach with mammal intestine mucosa and to grow in the presence of up to 0.4% of bile acid salts and to prevent the infection of intestine epithelial cells with rotaviruses. Agent shows the content from 1 x 105 to 1 x 1012 CFU of the strain L. paracasei I-2116 (NCC 2461) and can be used for preparing an agent and foodstuff taken among milk, yogurt, cheese, dry milk, children's nutrition products or a pharmaceutical preparation taken among a liquid bacterial suspension, dry oral supplement, liquid oral supplement, dry product for feeding through a tube or liquid product for feeding through a tube. Invention provides the enhanced viability of the strain in its using and effectiveness in prevention of adhesion to intestine cells and invasion of pathogenic microorganism cells in intestine causing diarrhea. Invention can be used in food industry and medicine in prophylaxis and/or treatment of diarrhea-associated diseases.

EFFECT: valuable medicinal properties of strain and agent.

3 cl, 5 dwg, 7 ex

FIELD: biotechnology, microbiology.

SUBSTANCE: invention proposes a method for preparing exopolysaccharide by culturing microorganisms in nutrient medium containing one or more carbon source assimilated by microorganisms and caruba seeds fraction as nitrogen organic source. Applying the proposed method provides preparing exopolysaccharide eliciting improved organoleptic, sensor and visual properties. Invention can be used in building, paper, textile, cosmetic, food, oil output industry and agriculture.

EFFECT: improved preparing method.

15 cl, 4 ex

FIELD: biotechnology, veterinary science, microbiology.

SUBSTANCE: the strain of bacterium Bacillus subtilis VKM B-2287 is isolated from soil. Bacterial cells are gram-positive with aerobic type of respiratory, don't form capsule but form round spores. The strain hydrolyzes glucose, mannitol and lactose but it doesn't ferment sucrose, inositol, sorbitol and maltose, it doesn't form gas in fermentation process and inhibits the growth of staphylococcus, Escherichia coli, enterobacteria, citrobacteria and aeromonas. The strain is used as industrial one for preparing a preparation named by authors as "Subtilis+". The preparation normalizes function of digestive tract in agricultural animals, poultries and fishes and can be used in treatment and prophylaxis of bacterial infections. Invention can be used for preparing the probiotic preparation.

EFFECT: valuable properties of strain and preparation.

1 tbl, 3 ex

FIELD: agricultural microbiology.

SUBSTANCE: the strain Azotobacter vinelandii IB 4 is obtained by analytical selection of natural isolates by method for selection of producers eliciting rather high antagonistic activity with respect to fungal phytopathogens. The strain is deposited in collection of microorganisms of Biology Institution UNTS RAN at number Azotobacter vinelandii IB 4. For preparing the preparation the strain is grown in nitrogen-free nutrient medium at 28-30oC for 60 h under aeration condition up to the titer value 1010 CFU/ml. The strain elicits high nitrogenase activity and ability for producing cytokinins that simulate the growth and development of plants.

EFFECT: valuable properties of microorganism strain.

7 tbl, 7 ex

FIELD: waste water treatment.

SUBSTANCE: cyanide effluents are treated with alkali or alkali-earth metal metabisulfite in presence of copper catalyst and residual cyanide and thiocyanate are subjected to bacterial destruction using strains Pseudomonas putida 21 and Pseudomonas stutzeri 18.

EFFECT: enabled detoxification of effluents within a wide cyanide and thiocyanate concentration range and therefore allowed use of the method for cleaning waste waters and slurries in gold mining, galvanic, pharmaceutical, and in a number of other industries.

FIELD: biotechnology, microbiology.

SUBSTANCE: invention relates, in particular, to preparing nutrient media used for culturing the plague microorganism vaccine strain and can be used in medicinal microbiology. The nutrient medium for culturing the plague microorganism vaccine strain comprises additionally as a stimulating additive sodium sulfite and as a nutrient base - soybean fruits enzymatic hydrolyzate in the following ratio of components, g/l: microbiological agar, 11.0-13.0; soybean fruits enzymatic hydrolyzate, 250.0-350.0; sodium chloride, 4.5-5.5; sodium hydrogen phosphate, 3.5-4.5; sodium sulfite, 0.0003-0.0005; distilled water, the balance. Invention provides enhancing the growth property of nutrient medium.

EFFECT: valuable properties of medium.

3 ex

FIELD: biotechnology, food and medicinal industry, microbiology.

SUBSTANCE: the strain Bifidobacterium longum 379-IN is obtained by selection without using methods of genetic modification of the strain Bifidobacterium longum B379M and distinct by ability to utilize insulin. The strain is deposited in GKNM GU "MNIIEM named for G. N. Gabrichevskiy Russia Ministry of Public Health" at № 172. The strain shows high technological effectiveness, accumulates biomass with substrates of vegetable origin and artificial nutrient media for short periods with concentration of bifidobacteria, it elicits acid-forming and antagonistic properties with respect to pathogenic and putrid microflora. This allows its using in manufacturing bacterial preparations, biologically active supplements for food, fermented-dairy and nonfermented-dairy foodstuffs, ferments, hygienic and cosmetic agents providing probiotic effect and normalization of microbiocenosis in human body, among them in gastroenteric and urogenital tracts, cutaneous and mucosa integuments. Invention can be used in manufacturing bacterial preparations, biologically active supplements for food, fermented-dairy and nonfermented-dairy foodstuffs, hygienic and cosmetic agents.

EFFECT: valuable properties of strain, expanded assortment of similar agents.

6 ex

FIELD: biotechnology, microbiology.

SUBSTANCE: method for preparing liquid lactobacterin involves regeneration, culturing passages of lyophilized culture and culturing ferment of lactobacilli in liquid lyophilized nutrient medium containing dry defatted milk enzymatic hydrolyzate with the content of amine nitrogen 1 485 mg%, 30.0 ± 3.0 g/l; yeast concentrated autolyzate, 110.0 ± 10 g/l; food agar, 0.8 g/l, and distilled water, up to 1 l. Culturing ferment is carried out up to accumulation of biomass of lactobacilli 109-1010 CFU/ml. Then 10-30% of supernatant liquid is removed from the ready product and replaced it with equal volume of fresh nutrient medium. Invention provides simplifying technology in preparing liquid lactobacterin and to elevate the storage period of viable lactobacilli. Invention can be used in producing probiotic preparations.

EFFECT: improved preparing method.

1 tbl, 3 ex

FIELD: biotechnology, microbiology, veterinary science.

SUBSTANCE: for preparing a preparation cells of microorganism Escherichia coli VKM CR-322D is cultured in nutrient medium containing Hottinger's broth, glucose, yeast extract, manganese sulfate, potassium hydrophosphate, sodium chloride and tap water in the content of amine nitrogen 125-155 mg%. Glucose is added by batch portions in the process of culturing cells that is carried out at temperature 30-31oC at stirring and aeration for 10-12 h. Prepared cultural fluid containing 3 x 109 bacterial cells/ml is mixed with protective sucrose-gelatin medium and subjected for lyophilic drying. Dried mass is stored under nitrogen that enhances safety of viable cells in the preparation. Applying the preparation for prophylaxis and treatment of agricultural and domestic animals and poultries with gastroenteric diseases provides its effectiveness.

EFFECT: improved preparing method, valuable veterinary properties of preparation.

17 cl, 8 ex

FIELD: biotechnology, microbiology, medicine.

SUBSTANCE: invention relates to the strain Lactobacillus paracasei CNCM I-2116 used for diarrhea prophylaxis causing by pathogenic microorganisms. Supernatant of this strain culture elicits ability to prevent colonization of intestine with pathogenic microorganisms causing diarrhea also and this strain is designated for preparing agent used for prophylaxis and/or treatment of disorders associated with diarrhea. Agent for oral administration represents therapeutically effective dose of the strain L. paracasei CNCM I-2116 or supernatant of its culture and acceptable foodstuff. Invention provides the enhanced viability of the strain in its applying and effectiveness in prophylaxis of adhesion to intestine cells and invasion to intestine cells of pathogenic microorganisms causing diarrhea.

EFFECT: valuable medicinal properties of strain.

5 cl, 8 dwg, 10 ex

Up!