Container for keeping allergenic disks

FIELD: medical engineering.

SUBSTANCE: device has bottom and cover joined in inseparable way so that no chance is available to mismatch covers belonging to different containers. The bottom and internal part of cover are lined with sponge so that the allergenic disks are kept in uniformly wetted state. Grate with holes is attached to lower sponge, the holes narrowing downward as cones so that the allergenic disks are kept in stable vertical position.

EFFECT: simplified access to disks using forceps; excluded misidentification risk.

5 cl, 6 dwg

 

The invention relates to a device for packaging and storage allergenic discs.

For chemical analysis of immunoglobulin E, or G, in addition to other reagents used in laboratories allergenic discs. They are round or polygonal disks of paper, a modified cellulose, plastic or similar materials. Some allergenic discs molded in the form of rings, by cutting the Central part. Typically, the disk diameter is from 0.5 to 1 cm, and the thickness ranges from 0.1 to 1 mm Such disks presented for sale in a large quantity.

Allergenic discs absorption or covalently coated with the appropriate antigen with which the antibody, for example, from serum, can form a connection that allows their identification in subsequent reactions. For example, during the determination of immunoglobulin E, or G, when the enzyme immunological examination allergenic disk forms a solid phase.

In connection with the specifics of their application, allergenic discs in many laboratories use in large quantities. As allergenic discs cannot be distinguished by appearance, they must be stored in such packaging, which provides easy access to drives and eliminates the possibility of erroneous identification. In addition, they should granites is in the wet state.

To date, allergenic discs were kept in glass bottles with a buffer solution or in plastic containers of the following types:

1. Plastic cassette containing a sponge immersed in buffer solution.

Allergenic discs have a hexagonal shape and are supplied in sheets, they can be separated from the sheet on specially marked perforation lines section. Packed in such a way allergenic discs are difficult to extract from the cassettes. In addition, the edges of allergenic discs may not come off exactly at the perforation.

2. Container with rigid foam (DE 8907465 U)

The bottom of the container is covered with a saturated buffer rigid foam with vertical grooves. Allergenic discs vertically inserted into these grooves. The disadvantage of such systems is that allergenic discs are in very close contact with a rigid foam particles which easily stick to allergenic discs, and this introduces errors in the results of subsequent measurements.

3. Plastic cassette with the inserted mesh

The bottom of the cassette is covered with impregnated buffer sponge. On top of it is a plastic mesh. Allergenic disk can be placed in any of the thus formed cell. The disadvantage of this tape is that it takes a lot of space, because allergenic disks storing the camping horizontally. In addition, they are difficult to remove with tweezers. There is a risk that the disc will slip into a neighboring cell.

Thus, all known devices have significant drawbacks hindering the rapid implementation of tests with allergenic discs.

The object of the present invention is to develop a device for packaging and storage allergenic discs, which provides greater ease of handling of allergenic discs, their compactness and storage without the risk of mistaken identity.

It was found that the requirements specified above conforms to the container with a hinged lid according to this invention. Preferably, the cover is not separated from the body of the container, because this design eliminates the possibility of confusing the lid from different containers. The housing and the cover are covered with a sponge, so allergenic discs maintain the same humidity. Grille with holes, which are made with small conical narrowing down, placed or fixed on the bottom of the sponge. Thus, allergenic discs are located in the holes vertically and stably. They can't slip off, and they are easy to remove with tweezers.

Consequently, the invention relates to a device for vertical storage allergenic discs, consisting of a body and a cover, characterized in that

a) the details of the housing and cover are covered with a sponge;

b) lattice with holes, which conically taper down, placed on the bottom part.

The preferred embodiment of the invention is a device in which the housing and the cover are connected to each other.

The preferred embodiment of the invention is a device in which the housing and the cover are connected by hinges.

The following preferred embodiment of the invention is a device that contains a cellulose sponge reinforced with natural fiber.

Another preferred embodiment of the invention is a device in which the cover and/or case provided with sealing edges.

Figure 1 shows a top view of the device according to this invention.

Figure 2 shows a side view.

Figure 3 shows a closed container according to this invention.

Figs.4, 5 and 6 shows the test results for storage allergenic discs in various types of packaging.

A Central role in the device or container according to this invention plays a lattice. It creates holes, which preferably have a square or diamond shape and conically narrowed down. However, allergenic discs can be inserted along the diagonals of the holes and let them fall down, until they will delay tapering down walls, or until they reach the bottom. Due to this layout is the position, allergenic discs cannot slip off or get in the neighboring holes. In addition, the disks can be easily removed, for example, tweezers because they are located in the container vertically.

The size of the holes may vary depending on the size of the allergenic discs. For most allergenic discs are suitable hole size 4×4 to 5×5 mm In the preferred embodiment of the invention is applied grating 27×27×27 mm, which has 25 holes the size of 4.45×4,45 mm (diagonal 6.3 mm), which are narrowed down to 4.25×4,25 mm (diagonal 6 mm). For storage in a container larger or smaller quantities of allergenic discs, you can change the number of holes in the desired manner so that the narrowing of the holes could be customized to the size of the allergenic discs.

In the preferred embodiment of the invention, the housing and cover of the container all the time connected to each other so that after opening the container, it is impossible to put on another cover, and this eliminates the error of identifying disks that can occur when the entanglement of covers from different containers. These two parts of the container are usually connected by hinges or other traditional mating parts, for example, a container with hinged lid, made as a single part. This can be, for example, a flexible flap p is avati, or other structures, usually made of plastic. Professionals in this industry there are various versions of such structures.

The bottom and lid of the container is covered with a sponge, which should be soaked in appropriate buffer solutions. Thus, allergenic discs remain sufficiently moist without contact with the sponge large city areas. Moreover, the use of double sponge prevents condensation effects on the body and slipping or sliding off allergenic discs.

Sponges usually pick up to the size of the bottom or cover and close them completely. Sponge or non-woven material, preferably composed of a hydrophilic natural or synthetic materials, such as cellulose, felt or latex, particularly preferably from pigmented cellulose, reinforced with natural fiber. Due to their ability to absorb liquid, sponges retain a sufficient quantity to maintain allergic drives in the wet state even without other liquid layers in the container. The conditions of storage in accordance with the principle of "wet chamber".

In the preferred embodiment of the invention, the lid and/or body of the container, particularly preferably cover, provided with a sealing edge. Thus, the container for this the invention can be well sealed, and allergenic discs retain moisture for a long time.

Figure 1 shows a top view of an open container according to this invention. The bottom and the lid D is covered with sponge S and connected by two hinged tacks V. Grating G is placed on the sponge, located at the bottom. She has narrowed down the hole, in which allergenic discs And can be inserted diagonally. Thus, allergenic discs are arranged vertically and are unable to slide into the holes, however, do not come into contact with the walls of the holes or sponge flat parts of its surface. After closing the valves, the container is maintained in closed condition by means of conventional fasteners R, located on the container and its lid.

Figure 2 shows a side view of the open container. Support lattice expands downwards and, thus, create a conically tapering holes. Depending on the size of the inserted allergenic discs slide into the holes or until until you come into contact with the sponge, or as long as they do not delay the tapering of the walls of the holes.

Figure 3 shows a container according to this invention in a closed form. Allergenic discs are surrounded by wet sponges, but not in contact with them large areas of the surface.

4, 5 and 6 are explained in more detail in the comparative example.

So about the time, the container according to this invention is free from the disadvantages mentioned at the beginning of the description:

Allergenic discs can be quickly and easily removed using tweezers.

They can't get stuck in the container.

- Due to the tight closure of the container and wet sponges on both parts of the container, allergenic discs moistened evenly.

- The vertical location of the openings, allergenic discs virtually no contact with the sponge, it means that they are unable to adhere particles of the sponge.

- Allergenic discs remain well fixed in their positions, even when transporting and turning the container.

- Preferred variant of the container according to this invention, in which the housing and the cover are connected, you can easily open and close without the risk of mixing up the cover.

Even if you do not specify other details, we can assume that the experts will be able to apply the above description in the widest sphere. Preferred embodiments and examples will be needed, therefore, is simply to complete the disclosure of the invention does not limited to.

Full disclosure contains all applications, patents and publications mentioned above and below and entered into this application by reference.

Comparative example

Figs.4, 5 and 6 the results of the test program is quality storage allergenic discs in the packaging of various types. Investigated the activity of allergenic discs with different allergens (d1, E1, g3, i1 and m2) after storage under different conditions. For testing, we selected the following package types:

1. The initial activity

For comparison, the initial activity of allergenic discs was determined directly after their manufacture.

2. Prototype

Allergenic discs were kept in the container for allergenic discs, known as the prototype (in accordance with DE 6907465 U),

3. This invention

Allergenic discs were kept in the container for allergenic discs according to this invention, which is shown in figure 1-3.

4. In dry atmosphere

Packaging was a closed glass bottle without moistened pads.

The above four types of packaging are presented in figure 4-6 blocks with the following identification keys:

The allergenic activity of the disks was determined by known methods by means of ELISA test, by determining the binding of IgE with the relevant allergenic discs. Activity (U/ml) was calculated using a reference curve based on known concentrations specific to the allergen IgE.

Figure 4 shows the activity of allergenic discs after storage for 32 weeks at a temperature of 2-8°C.

Figure 5 shows the asset is ity allergenic discs after storage for 32 weeks at a temperature of 25° C.

Figure 6 shows the activity of allergenic discs after storage for 8 weeks at a temperature of 36°C.

The test results show that, compared with other types of packaging, a container for allergenic discs according to this invention provides a more favorable storage conditions, in which allergenic discs keep their activity.

1. The storage device allergenic discs in a vertical position, comprising a container and a lid, wherein the container and the lid is covered with a sponge in a container placed grille with a conically tapering bottom holes intended to receive the disks.

2. The device according to claim 1, characterized in that the container and the lid are connected to each other.

3. The device according to claim 1 or 2, characterized in that the container and cover are connected by hinges.

4. The device according to claim 1 or 2, characterized in that the container and lid are covered with sponges cellulose, reinforced with natural fiber.

5. The device according to claim 1 or 2, characterized in that the cover and/or container provided with a sealing edges.



 

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