Method for estimating stress stability of kettle

FIELD: veterinary medicine.

SUBSTANCE: method involves interpreting dynamic omega potential behavior pattern relative to its initial level during 6-7 min after applying artificial pain irritation. The method is applied beginning from animal age of 1 month. Omega potential is measured before and after pain irritation test. Omega potential level growing down, the animal is considered to be of low stress stability. Omega potential level growing high, the animal is considered to be of high stress stability.

EFFECT: enhanced effectiveness of method.

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The invention relates to livestock and can be used in the selection of cattle in industrial technology, as well as to methods of breeding and selection of animals.

Numerous studies of domestic and foreign authors found that in the conditions of industrial technologies of production of milk and beef of animals are not able to fully adapt to the new conditions, thus reducing their productivity and reproductive ability, the timing of their economic use and increased morbidity. Therefore, the definition of adaptive capabilities of the organism is of great scientific and practical importance. Considerable practical interest is the assessment of stressoustojchivosti animal body.

Under stressoustojchivosti cattle understand the level of response of the animal organism to the influence of stress factors and its ability to adapt (adapt) to the new conditions.

At the present time to assess stressoustojchivosti and natural resistance are the following indicators (Chipledhunga, Wtederb, 1987):

biochemical content of total protein in serum inorganic phosphorus, calcium, carotene, vitamin a, glucose, ketone bodies, the catalase activity of the blood, alkaline reserve;

hematologica the Kie - the number of erythrocytes and leukocytes, hemoglobin content in the blood, morphological composition of blood cells, hematocrit, and others;

immunobiological - phagocytosis of leukocytes, an elimination properties of blood, antibody titer, bactericidal activity and the activity β-lysine, the authors estimated the effect activity of blood serum and other

These indicators make it possible to determine the reduction and even loss of protective-adaptive properties in animals and the natural resistance of the organism in the process of adaptation of cattle.

In accordance with the invention, to improve the efficiency of the method of evaluating stressoustojchivosti of livestock as a criterion deviation of omega-potential from its initial level during clockwise or counterclockwise after artificial painful irritation of the animal, and when the deviation clockwise in the direction of increasing omega-potential of the animals referred to vysokoustoychivy.

The analogue of our invention is to work Pro and Entozoa (A.S. No. 1819139, 1992), in which the authors propose to determine the resistance of cattle to use the amount of “stress sweat” on 1 cm2the area of the skin in the region of the last rib. The disadvantages of this method, in our opinion, are labor intensive is th costly, time-consuming and expensive equipment used to obtain the final result. Furthermore, the method has a relatively low reliability.

Also known technical solution (A.S. 1701215, class. And 61 In 5/04; And 01 To 67/02, 1991 - prototype), which use the initial level of omega-potential for determining the milk yield of cows. The disadvantage of this method is the lack of differentiation of animals by type of stressoustojchivosti within groups, resulting in and increased selection error that affect the accuracy of the method and the quality of the breeding herd.

The technical solution consists in increasing the efficiency, reliability and reduce the complexity of the method.

This object is achieved in that in the method of estimating stressoustojchivosti cattle, including the use of baseline omega-potential, an animal subjected to pain irritation and the deviation of omega-potential from its initial level during clockwise or counterclockwise determine the degree of stressoustojchivosti.

The novelty of the claimed proposal due to the fact that the first character of the deviations of omega-potential in artificial painful irritation from the baseline used to estimate stressoustojchivosti cattle.

The invention is illustrated precincts of the Ohm, where figure 1 shows the principle of assessing stressoustojchivosti animals on the nature of the deviation quantities of omega-potential from its initial level, figure 2 reflects theoretically expected and obtained empirically the nature of changes in omega-potential in animals with different stressoustojchivosti.

A specific example of the method of evaluating stressoustojchivosti cattle

In the comfort of the animal is fixed in the machine. According to the method Oissing and Art (1987) record the initial level of omega-potential of the animal. Then produce artificial painful irritation of the animal and observing the deviation of the readings for the measurement of omega-potential. Observations are recorded in the journal.

To prove the authenticity of the way we have conducted special studies in whose “Krasnodar” Kuban gosagrouniversiteta in 2001-2002

Experience 1. The purpose of this experience was to identify animals with the different nature of the changes to the original level of omega-potential after artificial painful irritation of the animal.

For the experiment it was tested on 50 cows of black-motley breed, similar in age. The animals were kept in the same barn with the same feeding. The research results are summarized in table 1.

5.00
Table 1

Changing the initial level of omega-potential after stimulation of the animal, mV
Periods, minVasoconstrictive (n=34)Nizkotexnologichno (n=16)
M±mσCv, %tdM±mσCv, %td
Baseline OP16,6±0,21,177,05-17,3±0,31,206,94-
0.3018,9±0,31,759,266,414,0±0,10,402,8610,4
1.0017,8±0,42,33to 13.092,215,4±0,31,207,794,4
2.0017,0±0,31,7510,291,616,2±0,41,609,881,6
3.0016,8±0,42,3313,870,416,4±0,31,20to 7.320,4
4.0016,7±0,21,177,010,216,5±0,10,402,420,3
16,6±0,10,583,490,416,6±0,20,804,820,4
6.0016,6±0,31,7510,540,017,0±0,31,207,061,1
7.0016,6±0,42,3314,040,017,3±0,20,80to 4.620,8

Analysis of the above data in table 1 shows that the experimental heifers on artificial stress factor react differently. Most animals (68%) after pain stimulation baseline omega-capacity first increases and then comes to the original metric. In other animals (32%), it decreases with subsequent recovery. The established differences are statistically vysokopostavlennyy (td=10,4 6,4...). Restore the original level of omega-potential in animals after stimulation and in the first and in the second case occurs for approximately 6-7 minutes.

We found that the change in omega-potential experimental heifers after artificial stimulation is curvilinear in nature. In the early period (first 3 minutes) its value in some animals mentioned above are initially recorded (1 type), and others, on the contrary, lower (2 type). Then PR is coming recovery readings prior to baseline values (IWAP). Moreover, the curves describing the dynamics of omega-potential as an empirical (experimental)and theoretical (calculated) have identical views. Therefore, in cattle there are two types of animals, estimated as extreme types vysokoustoychivy and nizkoresursnyh.

Experience 2. Set (Supplemento, Wtederb, 1987), which is the main indicator of stressoustojchivosti animal production conditions is the level of its productivity.

To this end, we conducted a production test method. The survey targeted two groups of cows of black-motley breed, 50 animals in each group. The formation of groups was made on the principle of steam-analogues by age, breed, body weight and duration of lactation. On average it amounted to 305 days. Feeding and maintenance of animals during the experience was the same. The results of the study are presented in table 2.

Vysokoustoychivy
Table 2

The relationship of the changes to the original level of omega-potential milk yield of cows
Group of cowsnThe milk yield for 305 days of lactation, kgr
M±MσCv, %td
505830,0±of 120.5723,012.4-+0,88
Nizkotexnologichno505308,3±126,5536,7the 10.12,99+0,83

Set (table 2)that milk yield highly stressoustoicivij cows was higher on 521,7 kg, or 10%, than their nizkoresursnyh peers in a highly reliable difference.

Stressoustojchivosti cows and their milk productivity have a high positive correlation (r≈0,9).

Thus, scientific experiment and its production testing allow to conclude that the nature of changes in the outgoing level of omega-potential in cattle after a painful stimulus reflects the stress of cows, and it can be used in breeding animals as a cheap quick way.

A method of evaluating stressoustojchivosti cattle, including the measurement of the physiological parameter of the animal after exposure to the stress factor, characterized in that as a physiological parameter using omega-potential, with pre-set to its original level after pain stimulation, increasing the level of omega-potential animal belongs to the very accousticly, while lower - to diskettestation.



 

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