Method for evaluating efficiency of trichophytosis therapy

FIELD: medicine, dermatology, clinical laboratory diagnostics.

SUBSTANCE: the present method deals with detecting the focus of neutrophilic phagocytic activity lesion in capillary blood. At the values of cells' capacity to phagocytosis in percentage and phagocytic number on the 10th d of therapy being below 20% and 3.3, correspondingly one should evaluate therapeutic efficiency to be low, if it is above 40% and 4.0, correspondingly - as high.

EFFECT: higher accuracy of evaluation.

2 ex, 3 tbl

 

The invention relates to medicine, namely to dermatology and clinical laboratory diagnosis, and can be used to assess the effectiveness of treatment of trichophytosis.

Known methods of evaluating the effectiveness of treatment on the clinical improvement of the patient, visual assessment of skin lesion (Pesterev PN. Trichophytosis zooantroponoznyh. - Tomsk: Izd. University, 1998. - 124 C.). Evaluating the effectiveness of treatment trihofitii carried out with the help of bacteriological seeding and microscopic diagnosis (Leshchenko V.M. Laboratory diagnosis of fungal diseases. - M.: Medicine, 1982. - 144 S.).

The prototype of the present invention is to evaluate the effectiveness of treatment using the definition of the functional state of phagocytes indicators phagocytic activity of the cells in venous blood. It is shown that there is a decrease of values in the initial period of the disease and the increase in the recovery period (Medvedev Y.A. Molecular-cellular mechanisms of immunogenesis in zoonotic trichophytia: abstract of Diss. Prof. the honey. Sciences. - M., 1988. - 36 S.). However, this method neutral state phagocytosis directly in inflammation, that does not fully assess the effectiveness of the treatment.

The technical result - improving the accuracy and reducing the time of the evaluation.

The decree of the config technical result is achieved by determining on day 10 of treatment ability of cells to phagocytosis in % the table of contents and phagocytic number of neutrophils capillary blood, taken directly from the lesion.

The proposed method is as follows: three times in the course of treatment (before treatment, at 7-10 days of treatment and at the end of treatment) investigated the capillary blood from the lesion. The blood samples are as follows: the skin at the site of blood collection (1-10 mm from the edge of the hearth) is treated with a solution of alcohol. Then the scarificator make a puncture of the skin. Pop drops of blood are collected in a test tube with heparin (15 μl) using an insulin syringe. The total amount of collected blood 40-60 µl. For the evaluation of phagocytic activity of neutrophils determine the ability of cells to phagocytosis (% content) and phagocytic number (the number of particles in a single neutrophil) (Laboratory research methods in the clinic: a Handbook / Menshikov V.V., Delektorskaya LN. - M.: Medicine, 1987. - 368 C.). When the values on the tenth day of treatment, the ability of cells to phagocytosis in % the table of contents and phagocytic number less than 20% and 3.0, respectively, the effectiveness of the treatment is assessed as low, more than 40% and 4.0, respectively, as high.

Properties of neutrophils in inflammation differ significantly from the properties of neutrophils circulating in the blood line. The presence of neutrophils in the blood is to last only way to move from a place of education (bone marrow) to the place of execution of the special function (t is Ani, the inflammation). Resting neutrophils are found in the blood, active in tissues or inflammation (Inflammation. A guide for physicians / edited WAV, Vsaugov. - M.: Medicine, 1995. - 640 C.). From the bloodstream neutrophils are recruited into a hotbed of potential threats, for example in the area of tissue damage, where they are activated and perform their function. Therefore, we have studied the performance of phagocytic activity of neutrophils obtained from inflammation (capillary blood) in patients zooantroponoses the trichophytosis and compared with similar indicators of cells circulating in the blood line (venous blood).

The results of the study patients zooantroponoses the trichophytosis are shown in table 1.

As can be seen from table 1, figures phagocytic properties of circulating cells and capillary blood before treatment was virtually at the same level. In the course of treatment was observed to increase performance phagocytic activity of cells of capillary blood 10 day (p<0.05) and after treatment (p<0,05). Venous blood was noted increase in performance only by the end of treatment (p<0,05), but they could not reach the capillary blood.

The authors in scientific medical and patent literature found no information about the determination of phagocytic activity of neutrophils capillary blood and in the lesion to assess the effectiveness of treatment trihofitii. Thus, the claimed invention meets the patentability criteria of “novelty”.

The research of the authors was first proved accurate estimates of the treatment trihofitii based on the phagocytic activity of neutrophils in the lesion. Thus, the claimed invention meets the criterion of “inventive step”.

The proposed evaluation method is illustrated by the following examples.

Example 1. The patient Was, 13 years old, was admitted to hospital in the Republican dermatovenerologic clinic complaining of a rash on the smooth skin. After the examination diagnosed with infiltrative trichophytosis smooth leather (42 source)caused by T. verrucosum. In addition to the General analysis of blood, the patient was taken blood from the lesion and venous blood for determination of phagocytic activity of neutrophils with the aforementioned method. The results are shown in table 2.

As can be seen from table 2, the indicators of phagocytic cells of capillary blood were on a higher level compared with venous blood and, unlike the data of venous blood were significantly increased in the treatment process. Growth was consistent with the clinical improvement, the tenth day of treatment the contours of the lesions were smoothed, the skin is much p is turned pale, infiltration slept, the elements of the rash began to be resolved. By the time of discharge from hospital (eighteen days of treatment) foci were not konturirovany, skin lesions regular color, peeling stopped. Consequently, a significant increase of phagocytic cells in inflammation on the ninth day of treatment corresponded to rapid clinical recovery.

Example 2. Patient F., 12 years old, was admitted to hospital in the Republican dermatovenerologic clinic complaining of a rash on the smooth skin. After the examination diagnosed with infiltrative trichophytosis smooth skin (31 hearth)caused by T. verrucosum. Also, as in the first patient, were collected blood from the lesion and venous blood for determination of phagocytic activity of neutrophils. The results are shown in table 3.

As can be seen from table 3, the performance of phagocytic cells of capillary blood Patient F. was significantly increased only in the period after treatment, although there was a trend towards growth on the tenth day of treatment. Indicators of venous blood was sharply decreased by the tenth day of treatment and increased by the end of therapy to the initial level. The clinical picture was observed more long-term resolution of lesions compared with the first case. The flattening elements, the resolution of infiltration and guipere the AI was observed for the twelfth day of treatment. The foci were konturirovany before the fifteenth day of treatment. At discharge, the skin lesions was normal in color, but remained easy peeling. Therefore, the absence of significant changes in the phagocytic activity of neutrophils in inflammation on the ninth day was accompanied by a long-term resolution of the disease, increase by the end of therapy consistent with the completion of the inflammatory process.

The proposed method was evaluated the effectiveness of treatment in 30 patients and in all cases was achieved the specified technical result. The method is easily reproducible in the hospital. Thus, the claimed invention meets the patentability criterion of “industrial applicability”.

A method of evaluating the effectiveness of treatment trihofitii

A method of evaluating the effectiveness of treatment trihofitii by determining the phagocytic activity of blood neutrophils, characterized in that the capillary blood of the lesion determine the ability of cells to phagocytosis in % the table of contents and phagocytic number and their values on the tenth day of treatment less than 20% and 3.3 respectively efficacy of treatment is assessed as low, more than 40% and 4.0, respectively, as high.



 

Same patents:

FIELD: medicine, biochemistry.

SUBSTANCE: in blood serum one should detect the level of lactoferrin and biliary acids. At their ratio being equal to 5-17 it is necessary to detect chronic hepatitis of high activity.

EFFECT: higher accuracy of detection.

3 ex

FIELD: medicine, infectology.

SUBSTANCE: one should detect the level of terminal stable metabolites of nitrogen oxide (NOx) in whole blood. At its value ranged 39.6-86.0 mcM/l one should evaluate hemorrhagic fever accompanied with renal syndrome (HFRS) of average severity, at NOx value ranged 86.7-141.5 mcM/l - severe form of HFRS and at its values ranged 88.2-128.6 mcM/l at the background of pronounced clinical picture - as complicated disease flow. The method enables to shorten the terms for carrying out the assays.

EFFECT: higher accuracy of evaluation.

3 ex, 1 tbl

FIELD: medicine.

SUBSTANCE: method involves studying blood samples with venous blood mixed with vital stain like methylene blue. Degree of vital stain absorption by erythrocytes is determined by applying photocolorimetry. The value drop being more than 25%, extracorporal detoxication is to be predicted as ineffective.

EFFECT: simplified method.

6 tbl

FIELD: medicine, analytical biochemistry.

SUBSTANCE: invention relates to laboratory methods of investigations. Method involves sampling specimen from patient to be inspected, extraction of serotonin and histamine from a specimen, chromatography of extract and determination of concentration of serotonin and histamine by the fluorescence intensity value. Saliva is used as biological fluid. Saliva by volume 1 ml is extracted with 4 ml of 1 N hydrochloric acid solution, 2 g of anhydrous potassium carbonate and 5 ml of mixture of butanol and chloroform in the ratio 3:2 are added, extract is shaken up and centrifuged. Organic phase (4 ml) is sucked off from extract and passed through chromatography column (diameter is 3 mm, height is 16 mm) filled with ion-exchange resin KB-4 or KB-4P-2 or Bio Rex-70 in H+-form, size of granules is 0.1 ± 0.02 mm. Histamine is eluted with 4 ml of 0.1 N hydrochloric acid at the rate of eluting solution 0.4 ml/min. Histamine concentration is determined by reaction with ortho-phthalic aldehyde dissolved in ethanol. Serotonin concentration is determined by reaction with ninhydrin in organic passed through column. Method provides assaying the saliva concentration of serotonin and histamine with high precision.

EFFECT: improved assay method.

The invention relates to medicine, namely to surgery

The invention relates to the encoded Micronesia, which is encoded using a stored code written by bleaching of fluorescent molecules on the surface of or inside microsites using impact Micronesian light radiation from a source with high spatial resolution

The invention relates to medicine, namely to laboratory diagnosis

The invention relates to the food and pharmaceutical industries and can be used in assessing the quality of medicines and biologically active additives to food with antioxidant properties; selection of optimum process conditions in the allocation of natural fat-soluble antioxidants; in search of an effective system of antioxidant synergists
The invention relates to medicine, in particular for dentistry

FIELD: medicine, analytical biochemistry.

SUBSTANCE: invention relates to laboratory methods of investigations. Method involves sampling specimen from patient to be inspected, extraction of serotonin and histamine from a specimen, chromatography of extract and determination of concentration of serotonin and histamine by the fluorescence intensity value. Saliva is used as biological fluid. Saliva by volume 1 ml is extracted with 4 ml of 1 N hydrochloric acid solution, 2 g of anhydrous potassium carbonate and 5 ml of mixture of butanol and chloroform in the ratio 3:2 are added, extract is shaken up and centrifuged. Organic phase (4 ml) is sucked off from extract and passed through chromatography column (diameter is 3 mm, height is 16 mm) filled with ion-exchange resin KB-4 or KB-4P-2 or Bio Rex-70 in H+-form, size of granules is 0.1 ± 0.02 mm. Histamine is eluted with 4 ml of 0.1 N hydrochloric acid at the rate of eluting solution 0.4 ml/min. Histamine concentration is determined by reaction with ortho-phthalic aldehyde dissolved in ethanol. Serotonin concentration is determined by reaction with ninhydrin in organic passed through column. Method provides assaying the saliva concentration of serotonin and histamine with high precision.

EFFECT: improved assay method.

FIELD: medicine.

SUBSTANCE: method involves studying blood samples with venous blood mixed with vital stain like methylene blue. Degree of vital stain absorption by erythrocytes is determined by applying photocolorimetry. The value drop being more than 25%, extracorporal detoxication is to be predicted as ineffective.

EFFECT: simplified method.

6 tbl

FIELD: medicine, infectology.

SUBSTANCE: one should detect the level of terminal stable metabolites of nitrogen oxide (NOx) in whole blood. At its value ranged 39.6-86.0 mcM/l one should evaluate hemorrhagic fever accompanied with renal syndrome (HFRS) of average severity, at NOx value ranged 86.7-141.5 mcM/l - severe form of HFRS and at its values ranged 88.2-128.6 mcM/l at the background of pronounced clinical picture - as complicated disease flow. The method enables to shorten the terms for carrying out the assays.

EFFECT: higher accuracy of evaluation.

3 ex, 1 tbl

FIELD: medicine, biochemistry.

SUBSTANCE: in blood serum one should detect the level of lactoferrin and biliary acids. At their ratio being equal to 5-17 it is necessary to detect chronic hepatitis of high activity.

EFFECT: higher accuracy of detection.

3 ex

FIELD: medicine, dermatology, clinical laboratory diagnostics.

SUBSTANCE: the present method deals with detecting the focus of neutrophilic phagocytic activity lesion in capillary blood. At the values of cells' capacity to phagocytosis in percentage and phagocytic number on the 10th d of therapy being below 20% and 3.3, correspondingly one should evaluate therapeutic efficiency to be low, if it is above 40% and 4.0, correspondingly - as high.

EFFECT: higher accuracy of evaluation.

2 ex, 3 tbl

FIELD: medicine.

SUBSTANCE: method involves studying blood serum, processing obtained data and setting disease diagnosis. The study is carried out by preparing dried blood serum sample as suspension in Vaseline oil and doing the infrared spectroscopy analysis in the bandwidth of 120-1000 cm-1 and determining absorption strip peak heights having maximum at 1180; 1165; 1160; 1150; 1130; 1070; 1025 cm-1 and then calculating the following two ratio groups, the first of which is ratio of peak height with maximum at 1165 cm-1 to 1150 cm-1; 1160 cm-1 to 1130 cm-1; 1070 cm-1; 1025 cm-1. The second group has ratio peak having maximum at 1165 cm-1 to 1160 cm-1; 1180 cm-1 to 1130 cm-1; 1065 cm-1; 1070 cm-1. The obtained three-dimensional distribution of the first group is projected to frontal plane for calculating two-dimensional coordinates and comparing to flat reference diagnostic images of hepatic pathologies and to a normal reference diagnostic image represented as flat polygons which boundaries are given by the following values. The norm is represented by X(-2.3;2.0;4.0;4.0) and Y(1.6;0.8;0.8;1.6), respectively. Oncology is represented by X(1.7;1.7;0.0;0.0) and Y(1.9;1.25; 1.25;1.9). Hepatites are represented by X(1.9;2.2;1.8;1.4 and 1.9;1.8;4.0) and Y(1.9;1.9; 0.5;0.5 and 08;0.5;0.8). Cirrhosis is represented by X(1.9;2.6;1.4) and Y(1.6;0.8;0.4). Diseases are differentiated by interpreting point position within particular area. Three-dimensional distribution of the second group is projected to frontal plane and compared to diagnosis images of pathology and norm. Coordinate values of the second group are as follows: norm - X(1.8;2.9;2.5;1.5), Y(2.7;2.0;1.2;1.6); oncological cases - X(0.27;0.67;0.63), Y(0.27;0.67;0.3); hepatitis - X(1.5;2.5;2.4;1.2), Y(1.6;1.2;0.2;0.9); cirrhosis - X(1.1;0.9;0.9). Final diagnosis of pathology is set when particular data values belong to the corresponding pathology zone in both cases.

EFFECT: high accuracy of diagnosis.

FIELD: medicine.

SUBSTANCE: method involves studying biological material by applying infrared spectroscopy techniques. The obtained data are processed and diagnosis is set. Blood serum is used as the biological material. The study is carried out by preparing dried blood serum sample as suspension in Vaseline oil and doing the infrared spectroscopy analysis in the bandwidth of 120-1000 cm-1 and determining absorption strip peak heights having maximum at 1170; 1165; 1160; 1150; 1140; 1060; 1050; 1040; 1025 and then calculating the following ratio values like peak height with maximum at 1160 cm-1 to 1140 cm-1; 1165 cm-1 to 1150 cm-1; 1040 cm-1 to 1025 cm-1. The obtained distribution of this group is projected to frontal plane for calculating two-dimensional coordinates and comparing to flat reference diagnostic images of prostate pathologies and to a normal reference diagnostic image represented as flat polygons which boundaries are given by the following values. The norm is represented by X(-1.15;-0.9;0.45;0.0;-0.65) and Y(0.99;4.2;0.9;0.46), respectively. Pathology by X(-1.15;-1.15;0.35;0.0;0.65) and Y(0.99;-0.03; 0.48;0.09;0.46). The norm and pathology are differentiated. Additional mathematical processing is carried out on infrared spectra of blood serum samples of patients belonging to pathology image according to parameter values. First of all, three-dimensional distribution is calculated as peak having maximum at 1160 cm-1 to one having maximum at 1150 cm-1; 1170; 1160 cm-1; 1160 cm-1 to 1025 cm-1. It is projected then to frontal plane and compared to diagnosis images of prostate adenoma and images of prostate carcinoma. The second group relationships the following values are used: oncological cases - X(0.28;0.77;1.24;0.96), Y(0.75;0.46;-0.13;-0.02); adenoma - X(0.28;1.24;2.21;1.24;0.77), Y(0.75;1.24;-0.12;-0.13;0.46). Differential diagnosis of pathologies is set by interpreting point position within particular pathology image.

EFFECT: high accuracy of differential diagnosis.

FIELD: medicine.

SUBSTANCE: method involves determining mean cytochemical coefficient of lipid accumulation in peripheral blood leukocytes in conditional units before beginning therapy application (MCC1) and in 2-3 or 5-6, or 10-12, or 20-24 months of therapy application (MCC2). Therapy effectiveness coefficient is calculated in conditional units from formula K= MCC2/MCC1. The value being equal to or greater than 1, leprosy therapy is predicted to be effective.

EFFECT: simplified prognosis method.

1 dwg, 1 tbl

FIELD: medicine.

SUBSTANCE: method involves determining infrared radiation absorption coefficient in blood plasma in bandwidth of 1543-1396 cm-1. The infrared radiation absorption coefficient is determined in %. The value being equal to 29.7±1.1%, catarrhal cholecystitis is diagnosed. The value being 26.4±1.4%, phlegmonous cholecystitis is diagnosed. The value being 21.2±1.8%, gangrenous cholecystitis is diagnosed. The value being equal to 18.6±0.5%, gangrenous perforated cholecystitis case is diagnosed. The value in norm is equal to 32.4±0.8%.

EFFECT: high accuracy and specificity of diagnosis.

FIELD: medicine.

SUBSTANCE: method involves pouring venous blood treated with heparin into five conic test-tubes in the amount of 0.1 ml. The first three of them contain 0.1 ml of non-colored latex suspension with particle size of 1.5 mcm, the fourth one contains 0.1 ml of medium 199 and 0.1 ml of 0.1% aqueous solution of tetrazole nitro blue, the fifth one contains .1 ml of latex suspension and 0.1 ml of 0.1% aqueous solution of tetrazole nitro blue. The first test-tube is incubated in thermostat for 5 min at37°C, the second one for 30 min, the third one for 1 h, the fourth and the fifth one for 40 min. Smears are prepared from 0.2 ml of incubation mixture on glasses and dried at 37°C, fixed in burner flame, stained with 0.1% aqueous solution of tetrazole nitro blue, repeatedly dried and studied with microscope under immersion with magnification of 90x10. Test results are evaluated from absorption activity in phagocytosis reactions in determining the number of phagocytes, phagocytic number, phagocytic integral index and phagocytosis rate values. Tetrazole nitro blue test response is determined by counting formazan-positive cell number, calculating cytochemical activity index and tetrazole nitro blue test stimulation index.

EFFECT: accelerated test; high accuracy and low cost of examination.

1 dwg, 3 tbl

Up!