Method for detecting hospital strains

FIELD: medicine, epidemiology, medicinal microbiology.

SUBSTANCE: method involves assay of sensitivity of strains to antibiotics, composing antibioticorgams and comparing antibioticograms of microorganisms isolated from patients and vicinity objects. Strain isolated from vicinity objects is diagnosed as hospital strain if it shows the resistance against three and more antibiotics and in the correspondence of antibioticograms of microorganisms isolated from patients and vicinity objects. Method provides carrying out diagnosis of hospital strains with the high precision. Invention can be used for detection of circulation of hospital strains and for carrying out anti-epidemic measures in curative-prophylactic institutions.

EFFECT: improved detecting method.

4 dwg

 

The invention relates to medicine, namely to epidemiology, to identify circulation of hospital strains in health care institutions (hospitals), and can be used for carrying out anti-epidemic measures in hospitals.

The problem of epidemiological surveillance in health care institutions in connection with the growth of purulent-septic diseases caused by hospital strains is important for clinics around the world.

Registered numerous cases of nosocomial infections (NI) in obstetric hospitals, surgical wards and outpatient clinics. Moreover, in the surgical wards of purulent-inflammatory processes were mainly caused by strains: E. coli, S.aureus, E.aerogenes.

To detect circulation of hospital strains in hospitals known methods of identification of microorganisms at different objects in hospitals, with additional verification of properties of selected micro-organisms, which include selected strains of microorganisms to the hospital (Sidorov, S. and co-authors. Hospital infections. “Antibiotics and chemotherapy”. - 1999. - 44. - 3. - S; Iskhakov HI, Bazhenov L.G. Hospital strains... in the surgical clinic. “The journal of Microbiology, epidemiology and immunology”. in 1991, No. 9. - P.27.). In microbiological PR is ctice to detect circulation of hospital strains are widely used epidemiological methods of labeling. Isolated from patients with strains using standard techniques identified to genus and species, and then spent intraspecific identification of bacteria with the aim of determining sarovara and biovars of the pathogen of nosocomial infections (Pramoxine NS, Morozova O.T. Evaluation of nosocomial infections and microbial colonization in newborns using epidemiological labeling strains of microorganisms involved. "Epidemiology and infectious diseases". - 1996, №3. - S. 24).

Known methods of detection of hospital strains of a long and complex.

The closest way to the same destination is selected as a prototype method for the diagnosis of nosocomial strain of Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA, which includes the determination of the sensitivity of the strain to antibiotics, fugative and serotype, resistance to disinfectants, plasmid profile, the coefficient of adhesion to epithelial cells, a strain of PSEUDOMONAS AERUGINOSA diagnoses as hospital in the absence of its sensitivity to nine or more antibiotics, the same phagocata, resistance to five disinfectants, similar plasmid profile and the coefficient of adhesion of 15±0.2 and more. (Patent RF №2110579, publ. 10.05.1998,)

For reasons that impede the achievement of specified following technical result when using known is on the way taken as a prototype, is that the known method requires considerable cost and long time (10-15 days) to produce the final results.

The task, which is aimed by the invention, is a simplified way of identifying nosocomial strains while reducing material costs.

The technical result is simple, does not require high material costs rapid method for the detection of nosocomial strains in the premises of health care facilities for diagnosing hospital strains with 100%accuracy.

This technical result in the implementation of the invention is achieved by a method of detection of nosocomial strains, including the determination of sensitivity to antibiotics of strains of microorganisms isolated from patients and from surrounding objects, according to the invention optionally use the drafting antibiotikum microorganisms and their comparison, strain, isolated from surrounding objects, diagnosed as a hospital with resistance not less than three antibiotics and compliance antibiotikum microorganisms isolated from patients and from the surrounding objects.

Distinctive features is that the proposed method is based on the compilation and mapping antibiotikum microor is Anisimov, isolated from patients with various items LPU, the coincidence which concludes circulation in a hospital facility hospital strain.

The proposed method, in comparison with the known, has the following advantages: reduces the time of detection of nosocomial strains in hospitals and saves material costs to conduct laboratory research.

The proposed method is as follows.

Allocated to culture microorganisms from different sources (from the body of the patient, and also from bakterionositelej or surrounding objects)that match their signs strains of S.aureus and E.aerogenes seeded on the surface of the medium.

On the surface of a nutrient medium with a suspension of microorganisms impose 4-6 disks impregnated with various antibiotics, such as penicillin, ampicillin, Cefazolin, gentamicin, amikacin, and others. The impact carried out for 18-20 hours at a temperature of 36°S, and then analyze the results.

The diameter of the zones of growth inhibition of taking into account the diameter of the disk is measured with an accuracy of 1 mm When measuring the zones of growth inhibition is focus on the complete inhibition of visible growth. Then make the antibiogram of resistance of microorganisms to antibiotics used in hospitals. Under antibiot cogram microorganisms of the same species, isolated from patients and from the surface of the surrounding objects, it is concluded that the circulation in hospitals hospital strain. The antibiogram must match at least 3 antibiotics.

Figure 1-4 shows a schematic of antibiotic resistance strains of microorganisms: figure 1 is a diagram of antibiotic resistance strains of S.aureus isolated from patients; figure 2 - scheme of antibiotic resistance strains of S.aureus isolated subjects in medical facilities; figure 3 - scheme of antibiotic resistance strains of E. aerogenes isolated from patients; figure 4 - scheme of antibiotic resistance strains of E. aerogenes allocated objects in medical facilities.

Neither figure 1-4 numbers 0-100 shows the percentage of resistance of the isolated strains of microorganisms to antibiotics.

When comparing the respective antibiotikum organisms S.aureus (1,2) and microorganisms E. aerogenes (3,4) on the basis of the identity of antibiotikum can conclude circulation in a hospital facility hospital strains.

The inventive method used on the basis of the surgical departments of the regional clinical hospital Tyumen.

A simple way to identify the circulation of hospital strains in hospitals and cheapness laboratory studies allow this method is widely used for surveillance in the PL is.

The method of detection of nosocomial strains, including the determination of sensitivity to antibiotics of strains of microorganisms isolated from patients and from surrounding objects, characterized in that it additionally making use antibiotikum microorganisms and their comparison, strain, isolated from surrounding objects, diagnosed as a hospital with resistance not less than three antibiotics and compliance antibiotikum microorganisms isolated from patients and from the surrounding objects.



 

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