Method for detecting both functional and metabolic state of nervous tissue

FIELD: medicine, neurology, psychopathology, neurosurgery, neurophysiology, experimental neurobiology.

SUBSTANCE: one should simultaneously register electroencephalogram (EEG) to detect the level of constant potential (LCP). At LCP negativization and increased EEG power one should detect depolarizational activation of neurons and enhanced metabolism. At LCP negativization and decreased EEG power - depolarized inhibition of neurons and metabolism suppression. At LCP positivation and increased EEG power - either repolarized or hyperpolarized activation of neurons and enhanced metabolism. At LCP positivation and decreased EEG power - hyperpolarized suppression of neurons and decreased metabolism of nervous tissue. The method enables to correctly detect therapeutic tactics due to simultaneous LCP and EEG registration that enables to differentiate transition from one functional and metabolic state into another.

EFFECT: higher accuracy of diagnostics.

5 dwg, 1 ex, 1 tbl

 

The alleged invention relates to medicine, namely, neurology, psychopathology, neurosurgery, neurophysiology and experimental neuroscience, and is intended to determine the functional and metabolic state of the nervous tissue.

There is a method of determining the state of the nervous tissue by registration of cerebral blood flow by hydrogen clearance using platinum electrodes implanted in the brain tissue or venous sinus /1/. However, the known method only indirectly allows to judge about the change in the functional and metabolic state of the brain and does not differentiate between many pathological and physiological state. How uninformative, difficult to implement, vasocongestion and hardly applicable in the clinic.

There is a method of determining the functional and metabolic state of nervous tissue by conducting positron emission tomography of the brain /2/. The disadvantage of this method is the complexity of implementation, high economic costs, and the use of expensive equipment for research, the need for preliminary training of patients to the study. The disadvantages of the method are the inability to conduct dynamic monitoring of the condition of the brain during medical manipulation is the third in the clinic, the complexity and inconvenience for the implementation of experimental studies in small laboratory animals.

The known method of registration level of constant potential (SCP) /3/ and electroencephalogram /4/. The SCP shifts reflect the polarization processes in nervous tissue /5/. Positive SCP shifts accompany the development of polarization processes (depolarization or hyperpolarization of neurons and glial cells, reducing the extracellular concentration of ions To the(+) and increase the concentration of ions of CA(2+) and Na(+) Negative SCP shifts reflect the development of depolarization processes (depolarization of neurons and glial cells, increased extracellular concentrations of K+, the decrease in the concentration of CA(2+) and Na(+)). However, the definition of the polarization processes does not allow to differentiate and detect the transition from one functional and metabolic state of the nervous tissue to another. The method does not allow to determine the pathological and physiological processes, has a low diagnostic accuracy and uninformative, which limits its application in clinical practice and experiment.

Closest to the proposed method is a method of registering a total of slow electrical activity of the brain from the surface of the scalp (EEG) /6/. It is known that the activation processes in the nervous system accompanied by depression alpha is aktivnosti. The development of pathological conditions due to a violation of the metabolism, as in ischemia of the brain, associated with the emergence of medlennovolnovoj activity in theta and Delta ranges. Inhibition of the functional state of the deepening of hypoxia and ischemia leads to depression EEG /7/, /8/, /9/. Despite having given way to a number of positive properties, it is not possible to subtly differentiate many physiological and pathological FS.

Thus, at present there are no specific methods to assess the functional and metabolic state of the nervous tissue in the whole range of physiological phenomena.

The objective of the proposed invention is to provide a method that improves the accuracy of determining the functional and metabolic state of nervous tissue by differentiation and registration of the transfer of one functional and metabolic state to another.

This object is achieved in that in the known method for determining the functional and metabolic state of nervous tissue by registering her bioelectric activity, simultaneously with a total of slow electrical activity (EEG), record levels of constant potential (PP), compare them, and the nature of changes of the measured parameters for the same period of time which determine the functional and metabolic state of the nervous tissue.

New to the achievement of the technical result is that in parallel with the registration of the constant potential record total of slow electrical activity of the nervous tissue and the changes in the measured parameters determine the functional and metabolic state of the nervous tissue. Simultaneous registration of the constant potential and total slow electrical activity allows the estimation of polarization and activation processes in nervous tissue, which increases the accuracy of determining the functional and metabolic state by differentiation and registration of the transfer of one functional and metabolic state of the nervous tissue to another.

Negativization SCP and the increasing power of EEG rhythms consider how the development of the depolarization activation of neurons and increase the metabolism of nervous tissue.

Negativization PP and reducing power of EEG rhythms is considered as the development of depolarization inhibition and inhibition of the metabolism of nervous tissue.

Positivization PM and the increasing power of EEG rhythms is considered as the development repolarization or hyperpolarizing activation of neurons and increase the metabolism of nervous tissue.

Positivization PP and reducing power of EEG rhythms is considered as the development hype the polarization inhibition of neurons and weakening of the metabolism of nervous tissue.

The method is as follows.

The object of the study nepostizhimaya (silver chloride) electrodes by using a DC amplifier conduct simultaneous registration of the level of constant potential and total slow electrical activity in the study of nervous tissue.

The enhancement or suppression of the total slow electrical activity and positive or negative changes of the level of constant potential determine the functional and metabolic state of the brain.

When a positive SCP shifts and the oppression of the EEG power development is determined by the polarization of the cell membrane (d - or hyperpolarization and a decrease in the metabolic needs of the nervous tissue. This functional condition develops or when hyperpolarizing inhibition, or when returning the membrane potential to resting potential after depolarization exaltation excitability.

When a positive SCP shifts and the increasing power of the EEG determine the development of the polarization of the cell membrane (d - or hyperpolarization) and the optimal level of metabolism of nervous tissue. This functional state is developing or by hyperpolarizing the exaltation of excitability, or repolarization in connection with the release of cells from metodicheskoe depression or namely parabiosis.

When the negative the main shifts SCP and the increasing power of the EEG determine the development of depolarization, accompanied by increased excitability and metabolic needs of the nervous tissue. These changes are observed, in particular, during the development of response activation.

When the negative SCP shifts and oppression capacity EEG determine the development of the depolarization braking (paraliticescom or katolicheskomu type) and decrease metabolism.

Example.

To assess the functional and metabolic state of the nervous tissue in our method, a study was conducted SCP and EEG in experimental acute circulatory ischemia of varying depths. Modeling ischemia was carried out in two ways on the same rats consistently within one experiment Pre-2-3 days prior experience outbred white rats weighing 150-200 g of either sex (n=12) under Nembutal anesthesia under the bones of the skull over the frontal cortex, the left and right hemispheres were implanted silver chloride electrodes. Indifferent chlorinated silver electrode was placed in the bone over the frontal sinuses. The findings of the electrodes was attached to the skull bystrootverdevayuschey plastic.

Thereafter, rats were operated under Nembutal anesthesia (40 mg/kg) about modeling ischemia. Registration bioelectrical brain activity in unipolar technique began before the introduction of anaesthesia and continued throughout the experiment is using a multi-channel DC amplifier with an input impedance of 1 Mω and bandwidth 0-40 Hz. Data ocifrovivaem (100 Hz) and for further processing into the computer. The definition of the spectrum of rhythms and its capacity was performed using Fourier transform. It was allocated five bands: Delta-1- (0.2 to 1 Hz), Delta-2- (1-4 Hz), theta (4-8 Hz), alpha (8-13 Hz) and beta (13-30 Hz) rhythm. SCP was averaged for the periods corresponding to the epochs of EEG analysis.

The first model of ischemia (Ischemia-1”) was bandaging both common carotid arteries. The duration of the isolated actions of this model of ischemia was 20 minutes, followed by an additional administration to rats of the occluder in the middle cerebral artery (MCA) of the left hemisphere (“Ischemia-2”) /10/. Combined modeling of two types of circulatory ischemia was carried out for 60 minutes, after which the occluder was removed and held registration SCP and EEG for 16 minutes.

In a separate series of experiments (n=10) conducted a study of SCP and power of the EEG after intracerebroventricular introduction of cyclopentylamine (LCA). The introduction of the LCA was carried out under General anesthesia (Nembutal, 40 mg/kg) by injection (25 mg/kg) in the middle cerebral ventricles of the right hemisphere.

Thus, there was an opportunity to assess the nature of changes of the complex bioelectric parameters (EEG and SCP) during anesthesia eleminal-sodium, intracerebral entricular the introduction of the LCA, ligature of common carotid artery, MCA occlusion and reperfusion and, based on data from the literature and obtained regularities of changes of bioelectric potentials, to determine the functional and metabolic state of the brain.

The results were processed statistically using the methods of parametric and non-parametric statistics for dependent and independent samples: criterion t Student, the criterion marks, Wilcoxon criterion, criterion of Wilcoxon-Mann-Whitney.

Figure 1 shows the change of power of the EEG and the PLO during simulation ischemia in two ways. It is seen that blocks the common carotid arteries (“Ischemia 1”) resulted in the increase of EEG power in the frontal and parietal cortex of the right and left hemispheres. The increase in the power spectrum of rhythms in all leads in General to the whole sample of rats was 14,04±1,75% (p<0,001). The greatest increase in amplitude (20,04±3,2%) was observed in the alpha range, where she grew up with 23,71±0,86 to 28,46±1,09 µv (p<0,001). For theta rhythm, the increase amounted to 16.31±3,15% and he has changed since 46,52±2.03 to 54,01±2,08 µv (p<0,01). The increase in the amplitude of the Delta rhythm was $ 10,61±2,79%: 106,85±3,96 to 118,38±3,74 µv (p<0,01). The least changed of the beta rhythm. Its capacity increased by only of 9.21±4,33% from 7.31±0,34 to 7.98±0,33 µv. However, and this increase was statistically significant (paired t-test Student showed what Alicia at p< 0,01). Simultaneously with the increase in the power of EEG rhythms was observed a small negative deviation SCP, which by the end of the period amounted to 1222,51±290,1 µv (p<0,01). According to published state of neural activation is accompanied by increased metabolism of nervous tissue /12/. The negative shift of the PP indicates depolarization of neural elements /9/, /7/, and in General with the increase of neural activity, this indicates that the development of nerve cells in the functional state type catelectrotonus.

Additional introduction of the occluder in the MCA of the left hemisphere on the background of General depression of EEG rhythms resulted in more significant changes SCP in the neocortex, which had both positive and negative direction (1, “Ischemia-2”). In the left hemisphere in the frontal and parietal cortex showed a significant (several tens of millivolts) negative deviation SCP. The average depression of EEG rhythms in the left hemisphere in the total sample was in the frontal cortex 25,62±2,41, in the parietal 22,74±1,94%. Most of the changes have affected the slow frequency (see table). In the right hemisphere oppression rhythms was significantly less than in the left hemisphere, and amounted to frontal cortex of 14.28±2,49% 13,03±2,19% for the parietal. Analysis of the EEG changes in the right hemisphere separately over a range of frequencies (see table) shows that ugnat the tion was affected only the Delta rhythm. In other frequency bands introduction of the occluder in the MCA of the left hemisphere did not lead to significant changes of rhythms, compared to the period prior to ischemia of the brain. Differences in changes of bioelectric potentials in the right hemisphere also dealt with the SCP. In the parietal cortex of the right hemisphere negative deviation was almost 3 times less than in the left hemisphere (see figure 2). In the frontal cortex of the right hemisphere deviation constant potential wore a generally positive direction.

Thus, the development of circulatory ischemia model 1 was accompanied by a relatively small negative deviation of the SCP and the increasing power of the EEG rhythms in all leads. Using model 2 of MCA occlusion of the left hemisphere resulted in significant additional negativization UPP left hemisphere and oppression it is against this background EEG. According to the literature /12, 13, 14, 15/ negative deviation SCP and depression EEG are indicators of the development of deep brain ischemia. This allows us to assume that in the left hemisphere when “Ischemia-2 was simulated severe ischemia of the brain, as confirmed by histological analysis /10/. Changes in EEG and SCP observed in the right hemisphere in Ischemia-2”connected, apparently, with redistribution of blood flow due to the activation of mechanisms of collateral circulation. In the literature, and euda data about the increase in stroke blood flow in the pool symmetric arteries of the opposite cerebral hemisphere /16/. We have observed in the frontal cortex of the right hemisphere positive SCP shifts testifies to increase of blood flow in this part of the brain, and reduce the power of the EEG rhythms reflects improvements in metabolic and functional state of the nervous tissue, worsened during Ischemia-1”. In other words, in the frontal cortex of the left hemisphere was formed near the preoperative. By increasing the blood supply to the frontal cortex, apparently, there was a partial “theft” of the parietal cortex of the same hemisphere. Therefore, in the parietal cortex of the right hemisphere after the introduction of the occluder in the MCA of the left hemisphere has also been negativespace SCP. The negative shift of SCP in the parietal cortex of the right hemisphere, coupled with some reduction in the power of rhythms indicates that here also during Ischemia-2” formed by relatively poor functional state of the nervous tissue.

Comparing the results of the changes of bioelectric potentials of the two models of ischemia allows us to consider “Ischemia-1” as the weaker model of ischemia. On similar models of ischemia shows /17/that, despite having the inactivation barrier transport system of the blood-brain barrier, neurons maintain a high level of oxidative metabolism due to the existence of, in particular, intracellular compensatory PR is sposabella mechanisms According to literature data, for hypoxic exposure neurons correspond to the primary reaction depolarization of the resting potential and activation of pulse activity, alternating with the deepening of hypoxia her depression by paraliticescom type /18/. Apparently, activation of the EEG and negativespace SCP observed when bandaging the common carotid arteries, and reflect the primary exaltation excitability of brain cells on carelectronics.com type.

Removing the occluder and reperfusion of the brain on the CME caused an increase in the power of EEG rhythms and positive deviation of SCP in the frontal and parietal cortex of the left hemisphere. A similar pattern was observed in the parietal cortex of the right hemisphere. In the frontal cortex of the right hemisphere MCA reperfusion of the left hemisphere has led to an increase in EEG power in the background negativization SCP. In other words, the recovery mode of the flow corresponding to the period of Ischemia-1” in the left hemisphere, electrophysiological data reflects improvements in metabolic and functional state of the nervous tissue. Similar processes took place in the parietal cortex of the right hemisphere, whereas in the frontal cortex removing the occluder, reducing collateral blood supply, worsened its metabolic condition that activated it again.

Data reperfusion indicate that brain activation observed show the Liu EEG, possible on the background of both negative and positive deviations of SCP. If in the first case, it indicates a worsening of functional and metabolic state (as with “Ischemia-1”), the second case is about improving it in connection with the exit status metodicheskoe depression while improving blood flow.

Thus, the use of two models of ischemia have shown that the development of ischemic processes associated with worsening functional and metabolic state of the neocortex, accompanied ambiguous changes in EEG. With a relatively weak semiii negativespace SCP, reflecting, as it is known, increasing depolarization neuroglial complex /7/, /9/, is accompanied by activation of the EEG. With the deepening of ischemia, even more negativespace SCP and depression of EEG rhythms, reflecting the development of the nervous tissue parabolicheskogo braking. Positive deviance SCP and the increased power of the EEG is observed during the reverse process: when the depolarization of neurons and output them, apparently, from the state namely parabiosis due to improved blood supply to the nervous tissue and its metabolic state.

Figure 3 shows the change UPP and EEG in rats after intracerebroventricular the introduction of LCA in the 4 leads. It is evident that the injection of the LCA caused in all cases, a positive deviation UPP the primary activation power of the majority (Delta-1, Delta-2 and beta) EEG rhythms, which then changed their depression. Most positiviste SCP was observed in the right hemisphere, i.e. on the side of drug administration. According to literature data, /19/, /20/ adenosine and its analogs (including LCA) inhibit the impulse activity of neurons and cause membrane hyperpolarization. Obtained in our experiment positiviste SCP also suggests that this deviation reflects the hyperpolarization of neurons. The hyperpolarization can be combined with the increasing power of rhythms and their oppression. Primary activation of the EEG (figure 4) in the Delta and beta bands in a positive shift of SCP reflects, apparently, the development of functional status, such anode exaltation, with increased metabolic needs against the conservation of ion homeostasis, which quickly changed to hyperpolarizing inhibition. Developed oppression capacity rhythms after the introduction of the LCA was stable and had over 60 minutes of observation.

The assessment of functional and metabolic state of the nervous tissue in our way was also conducted after the introduction of eleminal-sodium. The experimental design was as follows: after connecting the connectors with the wires from the amplifier to the terminals of the electrodes mounted on the head of the rat, the animal p which were located in the experimental chamber, where, after the calm within 5-10 minutes, they made the original recording of biopotentials, then the rat was taken in hand and with a syringe intraperitoneally injected eleminal-sodium (40 mg/kg). Figure 5 shows the variation in the recorded potentials intraperitoneal injection to rats eleminal-sodium. It is seen that immediately after the injection of the drug was observed adverse deviation SCP (p<0.001) and an increase of EEG power (p<0,001). After 1-2 minutes appeared positive displacement constant potential while maintaining high power rhythms (figure 5. “Predcon”). Loss of pain sensitivity and the falling asleep of the animal occurred against the background of further positivization UPP with a simultaneous decrease in the amplitude of EEG rhythms, oppression which reached a maximum during the deepening of sleep.

Comprehensive registration SCP and EEG during anesthesia allowed, therefore, to identify at least three successive stages of changes in the functional and metabolic state of the brain. Negative emotional arousal, obviously developing in animals during the puncturing needle of the skin and the drug was accompanied by negativities constant potential and the power increase EEG, reflecting, apparently, the development of depolarization exaltation neuronal excitability and increased metabolism n is rvnoy tissue. As absorption into the blood eleminal-sodium appeared positive deviation SCP while maintaining high-power EEG. The nature of the change of constant potential shows the development of the re - and hyperpolarizing processes. Increased the amplitude of the rhythms indicates the absence of another at this time hyperpolarizing inhibition. In the literature there is evidence of hyperpolarizing changes in the membrane potential of the neurons under the action of Nembutal /21/. Comparison of SCP and EEG characteristics allows us to consider the development of neurons in the period Predcon” functional state, the corresponding anode exaltation and strengthening during this period, the metabolism of nervous tissue. Finally, the onset and development narcotic sleep was accompanied by an even stronger positive shift of constant potential and the oppression of the power of the EEG. This functional state reflects, apparently, the deepening of hyperpolarization of the cells and the onset of hyperpolarizing inhibition with reduced metabolism of nervous tissue.

Comparison of these data with the results of the changes of bioelectric potentials with the introduction of the LCA indicates that there are fundamental similarities In both cases, the positive deviation of the SCP was accompanied by the initial activation of the EEG, with subsequent depression. It shows the, in both cases evolved in similar functional and metabolic processes.

The results are presented of experiments demonstrated the high diagnostic capabilities of the proposed method. Individually, neither EEG nor the SCP does not allow for subtle differentiation of functional and metabolic state of the nervous tissue. The advantage is the relative simplicity of the method. The proposed method allows to register and to differentiate the transition from one functional and metabolic state to another, thereby increasing the accuracy of diagnosis of pathological and physiological States, allows for adequate drug therapy of pathological conditions such as ischemia, aimed at restoring functional and metabolic state of the nervous tissue, and to determine prognosis and proper treatment after one or the other therapy, as well as to study the effect of extreme factors on the human body.

Thus, the proposed method makes it possible to accurately determine the functional and metabolic state of the nervous tissue, is adequate to differentiate between physiological and pathological conditions, to register the transition from one functional and metabolic state to another, which improves the accuracy of the diagnosis is IKI and highly informative way and allows you to properly define the tactics of treatment in neurology, psychopathology, neurosurgery and neurophysiology, as well as allows for the development of new pathogenetic neuroprotective drugs and to study the mechanisms of pathological and physiological conditions in the experiment.

Sources of information taken into account

1. Demchenko, ETC Methods of studying cerebral blood flow // research Methods blood circulation. Leningrad: Nauka. 1976, 104-125.

2. Buchsbaum MS, Gillin JC, Wu J, Hazlett E, Sicotte N, Dupont RM, Bunney WE. Jr. Regional cerebral glucose metabolic rate in human sleep assessed by positron emission tomography //Life Sci 1989, 45 (15): 1349-56.

3. Kohling R, A Schmidinger, Hulsmann S, Vanhatalo S. Lucke A, Straub H. Speckmann EJ. Tuxhom I, Wolf P, Lahl R, Pannek H, Oppel F, Greiner C, Moskopp D, Wassmann H. “Anoxic terminal negative DC shift in human neocortical slices in vitro,” Brain Res 1996 Nov 25, 741 (1-2): 174-9.

4. Ingvar D.H. Cerebral metabolism, cerebral blood flow end EEG//EEG Clin. Neurophysiol. 1967. Suppl. 25 P 102 to 106.

5. Marczynski TJ. Neurochemical interpretation of slow cortical potentials as they relate to cognitive processes and a parsimonious model of the mammalian brain. In: McCallum WC, Curry SH, editors. Slow potential changes in the human brain. New York: Plenum Press, 1993, p. 253-275.

6. The bioelectric potentials of the human brain. The mathematical analysis.// Edited Rusinova B.C., M.:Medicine, 1987, 254 S. (prototype).

7. Zhirmunskaya E.A. Electrical activity of the brain in normal, hypertensive disease and stroke. M, 1963.

8. Hockaday I.M., Potts F., Epstein E et al. EEG changes in acute cerebral anoxia from cordiac or respiratory arrest// EEG Clin. Neurophysiol., 1965. Vol. 18. No. 6. P.575-586.

9. Ingvar D.H., Sjolund B, Arbo A. Correlation between dominant EEG frequency cerebral oxygen upstake and blood flow// EEG Clin. Neurophysiol. 976. Vol.41, No. 3. P.268-276.

10. Supanova GS, Mustache L.A., Sofianou A.A., Shapkin A.G., Raevskaya LU, S. Golubev, Muriqi SE minimally Invasive model of focal cerebral ischemia in rats.// Experimental and clinical pharmacology 2001, v.64, No. 4, p.63-67.

11. Moskalenko J.E., Demchenko IT, Savich AA, Weinstein G.B. About the specifics of the ratio of local blood flow and some indicators of the functional status of limited parts of the brain. In the book: Correlation of blood supply to the metabolism and function. Edited Heimtextile, Tbilisi: back to text, 1969, s-163.

12. Komotini P.A., Chikvaidze, VN, Svanidze IK, Mchedlishvili GI Effect of ischemia on some metabolic processes in the Central nervous system. In the book: Correlation of blood supply to the metabolism and function. Edited Heimtextile, Tbilisi: back to text, 1969, s-210.

13. Gurvich, A. M., Chikunova L.G., Novoderezhkin I.S., Bulanova OLGA. The role of post-hypoxic changes of metabolism and brain oedema in the dynamics of recovery of functions of the Central nervous system after prolonged periods of complete cessation of blood circulation. In the book: Correlation of blood supply to the metabolism and function. Edited Heimtextile, Tbilisi: back to text, 1969, s-240.

14. Chen Q, Chopp M, Bodzin G, Chen H Temperature modulation of cerebral depolarization during focal cerebral ischemia in rats: correlation with ischemic injury. //J.Cereb Blood Flow Metab. 1993. May; 13 (3): 389-94.

15. Mies , Iijima T, Hossmann KA. Correlation between peri-infarct dementia DC shifts and ischaemic neuronal damage in the rat. //Neuroreport 1993 Jun; 4 (6): 709-11.

16. Pokrovsky A.V., N.N. Yakhno., Kuntsevich G And Lavrentieva M.A., Malkova M.B. features of intracerebral hemodynamics in patients with occlusive lesions of the main arteries of the brain. Journe. neuropath. and psychiatry, 1989, CH, vol. 9, p.7-11.

17. Dirlam - Reactivity of capillaries and pyramidal neurons of the cerebral cortex of rats in conditions of acute reduction of blood flow. Bull. the experts. Biol. and med., 1994, No. 5, s-560.

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Table

The power changing rhythms of different ranges in % during Ischemia-2" in relation to the period preceding the "Ischemia-1"
 The left hemisphere The right hemisphere
Delta-1The Delta 2ThetaAlphaBetaDelta-1The Delta 2ThetaAlphaBeta
Frontal cortex-50,83±3,52-38,10±5,07-34,10±2,41-11,76±4.09 to-8,45±6,22-51,86±2,44-23,45±3,17+3,45±2,20+1,39±2,62-7,18±5,94
Parietal cortex-44,84±of 4.38-21,77±3,31-21,17±3,31-13,03±2,90-17,09±5,20-42,94±1,60-18,35±3,24-4,94±3,39-2,63±3,43-4,23±1,89
""decrease, "+" - increase in the amplitude of rhythms

The method of determining the functional and metabolic state of nervous tissue, including the registration of the electroencephalogram (EEG), characterized in that simultaneously with the EEG record levels of constant potential (SCP) and negativization SCP and the increasing power of the EEG determine the depolarization of the neuronal activity and increased metabolism; if negativization SCP and the decrease in the power of EEG depolarization inhibition of neurons and inhibition of metabolism; if positively SCP and increasing Monastier - repolarization or activation of hyperpolarizing neurons and increased metabolism; if positively SCP and the decrease in the power of EEG hyperpolarizing inhibition of neurons and decreased metabolism of nervous tissue.



 

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