Method for preparing hollow horse-mint oily extract eliciting with antibacterial activity

FIELD: medicine.

SUBSTANCE: method involves using air-dried vegetable raw. Hollow horse-mint (Monarda) raw treated by steam is extracted with vegetable oil in the ratio raw : extract = 1:10 at heating for 5 h on water bath at temperature 60-70oC followed by filtration. Method provides the possibility for year-round manufacturing the hollow horse-mint (Monarda) oily extract eliciting with the high antibacterial and expressed anti-fungal activity.

EFFECT: improved preparing method.

1 tbl, 1 ex

 

Usage: the invention relates to medicine and can be used as antimicrobial agent of plant origin.

The aim of the invention is to develop a method of producing an oil extract from monardi fistular, possessing antimicrobial activity.

The new way is to obtain an oil extract of the dried plants of monarda fistular, establishing antimicrobial properties, not previously known for this plant.

A method of obtaining an oil extract from the aerial parts of Hypericum perforatum in the air-dry condition. The essence of this method lies in the fact that the crushed dry raw materials Hypericum perforatum steam, then pour vegetable oil in a ratio of 1:8 and extracted for 5 hours at a temperature of 70-80°C.

Thus obtained oily Hypericum extract exhibits antimicrobial activity against both gram-positive and gram-negative microorganisms. He has a strong effect on enterobacteria, already at a concentration of 30 mg/ml completely inhibited the growth of Enterobacter cloacae and Proteus vulgaris, and at a concentration of 50 mg/ml also completely inhibits the growth of Escherichia coli, Klebsiella pneumoniae, Citrobacter freundii, and Pseudomonas aeruginosa.

The lack of an oil extract of St. John's wort is its lower activity against graphology the nutrient microorganisms. None of the concentrations it does not inhibit completely the growth of Staphylococcus aureus (St. aureus and Enterococcus (Str. faecalis), although it reduces its (the highlands of GI, Volhonska T.A., Yakimova UL, Debt V.I. "the Way to obtain an oil extract having antimicrobial activity". Patent 2205017. 2003. Bul).

Oil extract obtained from monardi fistular shows high activity against gram-positive microorganisms. Already at a concentration of 30 mg/ml, it completely suppresses their growth.

The technical result of the invention consists in providing a method to obtain an oil extract from monardi fistular, possessing antimicrobial activity.

Bee balm is fistular (Monarda fistulosa L.) is a herbaceous plant of the family Lamiaceae. It is native to the Northern America, whence it was brought to Spain in the 16th century. In Russia, bee balm is found only in the conditions of culture in the European part of Russia, in the Urals and in Siberia. Medicinal, perfumery and food value of monarda due to the content in plant essential oils exhibiting antibacterial, anthelmintic, antibiotic, immunomoduliruushim activity. The period of maximum accumulation of these biologically active substances is the flowering period. Our research has shown that essential oil of the flowering plants contains about 40 components, and output it with what is 3,4-4,2% (Oparin W., Pokrovsky L.M., Vysocina GI, Tkachev A.V., Study of chemical composition of essential oils of Monarda fistulosa L. and M. didyma L., cultivated in the conditions of Western Siberia. 2000. No. 3. P.19-24. Publishing house of the Altai state University. Barnaul). Yalta Institute of physical methods of treatment and medical climatology has certificates of authorship on the opening on the use of essential oils of monarda as a radioprotective agent for the treatment of asthma, chronic bronchitis and tracheitis, as a means of facilitating engraftment of alien tissue, as preservative blood. Essential oil of monarda promotes treatment of diseases caused by infection with Salmonella. It is effective against protozoa - Trypanosoma and amoeba (Dragana IV, Kahn LU Secret healing properties of monarda. "Potatoes and vegetables." No. 5. 1997).

In monarda present, in addition to essential oils, and other biologically active substances, such as flavonoids with strong antiseptic and anti-inflammatory action. Along with essential oils, flavonoids plant obliged manifestation of efficacy against various pathogens (bacteria, fungi, protozoa, and others). The maximum number of flavonoids contained in the aboveground parts of monarda fistular in the phase of mass flowering (1,5-2,0%) (according to authors). In practice, the use of infusions, pulp, juice of leaves and inflorescences of Monard is, which contribute to the treatment, epithelization and granulation nonhealing wounds, eczema, cuts, abrasions, help fight seborrhea, dandruff, acne, flaky skin. In practice it is more convenient to use an oil extract of monarda than infusions, gruel or juice that have a short shelf life and can be produced only from fresh plants.

This objective is achieved in that the crushed air-dry raw material (length stems not more than 2 cm) steam on steam for 10 minutes. Steamed raw material is transferred into the container, pour refined sunflower oil at a ratio of 1:10 and warm on a water bath for 5 hours at 60-70°C. After 5 hours, the raw material is press-ready oil extract is filtered, the filtrate poured into dosed containers (bottles).

Example. 100 g of powdered air-dried aerial parts of monarda fistular steam out on the sieve with one layer of white baseway or cotton fabric in a boiling water bath for 10 minutes with closed lid. Steamed raw material is transferred into the container, pour 1000 ml of refined sunflower oil and heat on a water bath for 5 hours at 60-70°C with stirring. Oil extract is filtered through a Buechner funnel with basevi filter under vacuum. The raw material is squeezed and the remains of an oil extract add in Ronco for filtering. Transparent filtrate yellowish-green in color and poured into a metered capacity (bottles of 50 or 100 ml).

Antimicrobial activity

Antimicrobial activity determined in a known manner (Ministry of health of the USSR №535 from 15.04.85 year). In tubes containing 1 ml mycopathologia broth, bring the tested microbial strains, incubated at 37°C for 24 hours. Get day cultures of the strains tested. In a test tube with daily culture make oil extract to a final concentration of 30 and 50 mg/ml, incubated them at room temperature for 3 hours, shaking occasionally. Then produce sowing on Petri dishes, which are placed in a thermostat at a temperature of 37°C for 24 hours. The number of grown colonies on plates appreciate the signs +:

++++ not read the number of colonies;

+++ -150-100 colonies;

++ -50-100 colonies;

+ -less than 50 colonies;

- lack of growth.

Data on antimicrobial activity of the oil extract is presented in the table .

Antimicrobial activity of the oil extract of monarda fistular
Types of microorganismsControlConcentration
30 mg/ml50 mg/ml
Staphylococcus aureus++++--
Streptococcus faecalis++++--
Escherichia coli+++++-
Enterobacter cloacae++++--
Proteus vulgaris++++--
Klebsiella pneumoniae++++--
Citrobacter freundii+++++-
Pseudomonas aeruginosa+++++-
Candida albicans+++++++

From the data presented in the table on the study of antimicrobial activity of the oil extract of monarda fistular we can conclude the following. Already at a concentration of 30 mg/ml, it completely inhibited the growth of Staphylococcus aureus, Streptococcus faecalis, Enterobacter cloacae, Proteus vulgaris and Klebsiella pneumoniae, oppressing the rest of microorganisms and Candida albicans. At a concentration of 50 mg/ml there is a complete suppression of the growth of all microorganisms and even greater inhibition of Candida albicans.

Thus, the claimed method to obtain an oil extract of monarda fistular, having antimicrobial activity, can be used for year-round production of antimicrobial agent in a wide range of therapeutic actions.

The use of the method of obtaining oil extract and is monardi fistular, possessing antimicrobial activity in comparison with other ways provides the following expected benefits:

1. Allows the source to obtain an oil extract to use monardo fistular in the air-dry condition and to release its uninterrupted throughout the year.

2. Allows you to release therapeutic agent from the antimicrobial activity of a wide spectrum of deystviy.

3. Allows you to confirm the feasibility of the industrial cultivation of monarda fistular in Siberia.

The way to obtain an oil extract having antimicrobial activity, including grinding, steaming dry plant materials steam, extraction of vegetable oil for 5 h and filtered, characterized in that as the plant materials used aboveground part of monarda fistular, collected in the period of maximum accumulation of biologically active substances that lead to antimicrobial activity, decoupling should be performed within 10 min, the extraction is carried out at a ratio of raw material : solvent 1:10, respectively, at 60-70°C.



 

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7 cl, 4 tbl, 43 ex

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EFFECT: improved preparing method.

2 tbl

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