Method for protection of vegetative solanaceous plants from insect pests

FIELD: plant production.

SUBSTANCE: method includes spraying of vegetative solanaceous plants with Steinermena feltiae suspension in combination as antidesiccant with agent obtained from biomass of Mortierella jenkinii micromycete according to claimed technology.

EFFECT: insect pest control with improved effect.

 

The invention relates to technology for biological protection of plants.

There is a method of vegetative protection solanaceous plants from harmful insects, providing them spraying the suspension Steinernema caprocapsae with the addition of antigenicity in the form of a preparation it includes (Pushnia M.V., Technology for entomopathogenic microsporidia and nematodes and their application for the protection of solanaceous crops. Abstract of thesis. Ph.D. - Krasnodar: VNIIBT, 2000, p.14).

The technical result of the invention is the extension of the range of agents for biological protection of plants from harmful insects and effectiveness of protection.

This result is achieved that the way to protect vegetative solanaceous plants from harmful insects, which they spraying a suspension of the entomopathogenic nematodes with the addition of antidetonant, according to the invention as entomopathogenic nematodes using Steinernema feltiae, and as antidetonant drug use, obtained by sequential extraction of biomass micromycete Mortierella jenkinii nonpolar solvent in the supercritical state, water, alkali, water, acid, water, alkali and water followed by combining the first extract from the solid residue, in amounts of 0.2-1% by weight of the suspension.

The method is implemented as follows.

Dry is iomass micromycete Mortierella jenkinii extracted with non-polar extractant, for example carbon dioxide or hexane, supercritical condition. At this stage, separating the first extract used in the future when receiving the drug. Further biomass sequentially extracted with water, alkali, water, acid, water, alkali and water. Obtained after completing all these stages of extraction of the solid residue combined with the first extract. Thus obtained product contains, mainly, chitosan and higher polyunsaturated fatty acid that provides the possibility of its use as antigenicity. In the drug no phyto-toxic substances that can be used in the processing plants. The drug is administered in a suspension of the entomopathogenic nematodes Steinernema feltiae in amount of 0.2-1% by weight of the suspension. The amount of preparation of the biomass micromycete Mortierella jenkinii justified by the fact that when its content in the suspension is less than 0.2 mass% of its antigenicity properties become insignificant to achieve a technical result, and when the content of more than 1% by weight dramatically increases the viscosity of the suspension, which when the content of the drug is about 1.5% starts heliroute, which complicates the use of the suspension according to the intended purpose.

Vegetative plants of the Solanaceae family, such as tomatoes, potatoes, eggplant, Oprah is nodding freshly prepared composition suspension Steinernema feltiae and preparation of biomass micromycete Mortierella jenkinii. Processing should be done with the mass emergence of larvae of harmful insects in the evening period in the absence of precipitation. The time to treatment is justified by the fact that this part of the day is characterized by lower temperatures and lack of sunlight, which prevents nematodes from drying out and death. The absence of precipitation guarantees the preservation of nematodes on the green mass of plants in areas where the pests. Viable nematodes infect insects, causing them to die. They nematodes selected not harm vegetating plants.

To confirm the achievement of the technical result was a series of experiments in laboratory and field conditions. In laboratory conditions, suspensions of nematode species Steinernema caprocapsae and Steinernema feltiae was carried out by infecting the most dangerous pest of solanaceous crops - Colorado potato beetle larvae, number 25; 50 and 100 nematodes per larva. The death of the larvae of the pest first age was 90-100%, of the second age 60-100%, the third age 90-96% when the number of infection 50-100 nematodes per larva and 10-18% of the larvae of the fourth age with the same number of infection, regardless of the type of nematodes. Field tests conducted on potatoes, struck the Colorado potato beetle. When the flow of the suspension of nemato the 8· 105instances with the addition of 0.01% A.I. of Biostate or 0.2 or 1% of the drug from biomass Mortierella jenkinii 1 m2landing on the closest analogue and the proposed method respectively obtained efficiency after 1 day 72.1% of the known method is 83.8% and 82.9 per cent to declare three days 74,8%; 87,9% 88,2%, respectively. This is probably due to less loss of nematode species Steinernema feltiae in composition with the preparation of the biomass micromycete Mortierella jenkinii than nematode species Steinernema caprocapsae in composition with Biostator, which may be caused or better antideslizante properties of the drug from biomass micromycete Mortierella jenkinii compared with Biostator, or a smaller content of substances toxic to the nematode species Steinernema feltiae than Biostate toxic to species of Steinernema caprocapsae.

At the same time on the experimental plots revealed reduction of fungal diseases and increase yields of potatoes in comparison with the reference, which is probably due to the presence of the drug from biomass micromycete Mortierella jenkinii substances that stimulate the growth and defence reactions of plants.

Thus, the proposed method can improve the effectiveness of pest insects on vegetative solanaceous crops.

The way to protect vegetative solanaceous plants from harmful insects, providing their OpRisk is of the suspension of the entomopathogenic nematodes with the addition of antidetonant, characterized in that as entomopathogenic nematodes using Steinernema feltiae, and as antidetonant drug use, obtained by sequential extraction of biomass micromycete Mortierella jenkinii nonpolar solvent in the supercritical state, water, alkali, water, acid, water, alkali and water followed by combining the first extract from the solid residue, in amounts of 0.2-1% by weight of the suspension.



 

Same patents:

FIELD: plant production.

SUBSTANCE: method includes potato treatment before dispatch for storage with adjuvant in combination with 0.2-1 mass % of Steinermena feltiae suspension. Adjuvant is obtained from biomass of Mortierella gemmifera micromycete according to claimed technology.

EFFECT: insect pest controlling with improved effect without detrimental effect on potato immunological status, germinating, and organoleptic characteristics.

FIELD: plant production.

SUBSTANCE: method includes potato treatment before dispatch for storage with adjuvant in combination with 0.2-1 mass % of Steinermena feltiae suspension. Adjuvant is obtained from biomass of Mortierella hydrophila micromycete according to claimed technology.

EFFECT: insect pest control with improved effect without detrimental effect on potato immunological status, germinating, and organoleptic characteristics.

FIELD: plant production.

SUBSTANCE: method includes potato treatment before dispatch for storage with adjuvant in combination with 0.2-1 mass % of Steinermena feltiae suspension. Adjuvant is obtained from biomass of Mortierella jenkinii micromycete according to claimed technology.

EFFECT: insect pest control with improved effect without detrimental effect on potato immunological status, germinating, and organoleptic characteristics.

Sandwich paste // 2243703

FIELD: food-processing industry.

SUBSTANCE: sandwich paste includes liver, pork cuttings, dry protein semi-finished product, dry milk, chick-pea, rice, beet, marrow, CO2-extracts of laurel leaves, eugenol basil, dill, preparation produced from Mortierella pulchella micromycet biomass by predetermined process, edible salt and water.

EFFECT: harmonized organoleptical properties of product and improved assimilation of nutritive substances.

Sandwich paste // 2243702

FIELD: food-processing industry.

SUBSTANCE: sandwich paste includes liver, pork cuttings, dry protein semi-finished product, dry milk, chick-pea, rice, beet, marrow, CO2-extracts of laurel leaves, eugenol basil, dill, preparation produced from Mortierella parvispora micromycet biomass by predetermined process, edible salt and water.

EFFECT: harmonized organoleptical properties of product and improved assimilation of nutritive substances.

FIELD: food-processing industry.

SUBSTANCE: meat-and-vegetable paste includes liver, pork cuttings, dry protein semi-finished product, dry milk, lentils, rice, beet, marrow, CO2-extracts of laurel leaves, eugenol basil, dill, preparation produced from Mortierella nantahalensis micromycet biomass by predetermined process, edible salt and water.

EFFECT: soft taste and aroma, improved assimilation of nutritive substances.

FIELD: food-processing industry, in particular, sandwich paste composition.

SUBSTANCE: meat-and-vegetable paste includes liver, pork cuttings, dry protein semi-finished product, dry milk, lentils, rice, beet, marrow, CO2-extracts of laurel leaves, eugenol basil, dill, preparation produced from Mortierella spinosa var. sterilis micromycet biomass by predetermined process, edible salt and water.

EFFECT: soft taste and aroma, improved assimilation of nutritive substances.

FIELD: food industry.

SUBSTANCE: the present innovation deals with technology of manufacturing frozen products out of fruit-berry raw material. Method to obtain frozen dessert deals with preparing fruits and/or berries followed by their reducing, addition of granulated sugar, carraginan and a solid residue. The latter should be obtained after extracting Pythium ultimum micromycete biomass by the preset technique. Then they should be mixed at heating at simultaneous inactivation of native enzymes, cooled followed by addition of mycelium separated after the first stage of extraction of Pythium ultimum micromycete biomass with liquid carbon dioxide at simultaneous increase of pressure, then pressure should be decreased up to atmospheric one to obtain the target product in its frozen form. The present innovation enables to decrease energy capacity and accumulation of melanoidins in the target product.

EFFECT: higher efficiency.

FIELD: food industry.

SUBSTANCE: the present innovation deals with technology of manufacturing frozen products out of fruit-berry raw material. Method to obtain frozen dessert deals with preparing fruits and/or berries followed by their reducing, addition of granulated sugar, carraginan and a solid residue. The latter should be obtained after extracting Mortierella indohii micromycete biomass by the preset technique. Then they should be mixed at heating at simultaneous inactivation of native enzymes, cooled followed by addition of mycelium separated after the first stage of extraction of Mortierella indohii micromycete biomass with liquid carbon dioxide at simultaneous increase of pressure, then pressure should be decreased up to atmospheric one to obtain the target product in its frozen form. The present innovation enables to decrease energy capacity and accumulation of melanoidins in the target product.

EFFECT: higher efficiency.

FIELD: food industry.

SUBSTANCE: the present innovation deals with manufacturing fruit-berry puree by applying additives out of a microorganism. One should perform the following technique: raw material should be rubbed through without separating waste products, decanted to mix centrifugate with precipitation and a solid residue obtained after extracting Pythium insidiosum micromycete biomass in the preset sequence to be repeatedly decanted. Then one should supplement the centrifugate with an extract obtained after the first extraction of the same biomass with a nonpolar extragent in supracritical state to be heated, packed and thermally treated at obtaining the target product.

EFFECT: increased quality of the target product.

3 ex

The invention relates to the field of Microbiology and can be used as a means for protection of plants from diseases and pests

The invention relates to agricultural biotechnology, in particular to the production and use of biological means of combating plant pathogens

The invention relates to agriculture, in particular to the field of plant protection from pests and diseases, and can be used to prevent the development of resistance of Colorado potato beetle to insecticides

The invention relates to the microbiological industry

The invention relates to the microbiological industry, and in particular to methods and lines for the production of biological products based on entomopathogenic nematodes used as biological agents in the control of insect pests
Up!