Method for preventing immunodeficiency in experiment

FIELD: medicine.

SUBSTANCE: method involves exposing thymus to microwaves of millimetric bandwidth and wavelength equal to 5.6 mm. Single exposure time is equal to 1-2 min with total number of procedures, given every second-third day, being equal to 6. Immunodeficiency model is reproduced at the second day after having accomplished the procedures by combining hexenal anesthesia and abdominotomy followed by immobilization stress accompanied with immunological value control 5 days after reproducing the model.

EFFECT: enhanced effectiveness of immunodeficiency prophylaxis under experimental conditions.

1 tbl

 

The invention relates to the field of medicine and relates to methods of prophylaxis of disorders of the immune status of physical methods.

Closest to the claimed method is prevention of postoperative immunodeficiencies (RF patent No. 2185216, registered in the state register of inventions of the Russian Federation of 20 July 2002, the authors by V. Melnikov, Umesi and others).

The known method is that the fact that before operational intervention effect of ultrasound on labile methodology to the area of the handle of the sternum, daily pay exposition 2-2,5 minutes to the total number of procedures is equal to 4-6.

The disadvantage of this method is that its application of ultrasound on the above region is limited by a number of contraindications (pathology of the thyroid and parathyroid glands expressed vegetative disorders, widespread atherosclerosis of the coronary arteries and aorta etc). In addition, the efficiency of monopropylene on phenotypic and immunological parameters (E-ROCK various modifications, antibody productive cells) is not always sufficient.

The proposed method devoid of the above disadvantages. How is that carried out first impact on the region of the thymus microwave millimeter-wave with a wavelength of 5.6 mm, a single exposure for 1-2 minutes when the total number of p is ocedur - 6 through one or two days, the next day after completion of procedures reproduce the model of immunodeficiency.

The method is as follows. Under the control parameters of the immune status of animals, such as rats, first by the irradiation of the specified area microwave millimeter-wave with a wavelength of 5.6 mm, a single exposure for 1-2 minutes when the total number of procedures 6 through one or two days. The next day after completion of procedures reproduce the model of immunodeficiency, for example, by combining geksenalovy anesthesia (introduction depending on the body weight of 0.3-0.8 ml of 5% solution geksenala intraperitoneally), abdominator (skin incision, fascia and peritoneum over the white line with a length of 2-3 cm with the subsequent imposition of appropriate joints) and immobilization stress (immobilization of individual cells burrows at night). After 5 days of conducting the monitoring of immunological parameters.

Studied 40 rats male Wistar rats weighing 150-200 g were divided into 4 groups of 10 animals. 10 rats of the first group served as intact control for other animals.

The 10 animals of the second group played the model of immunodeficiency States. When this was initially carried out by intraperitoneal injection, depending on body mass average of 0.5 ml of 5% solution geksenala intraperitoneally. After 5-10 minutes at the height of the dream is proizvodila skin incision, fascia and peritoneum over the white line with a length of 2-3 cm with the subsequent imposition of appropriate seams. Then the animals were obezdwijivanie in individual cells burrows at night with the purpose of reproduction immobilization stress. After 5 days spent monitoring of immunological parameters.

10 animals of the third group pre-made course of the impact of low ultrasound power on the area of the thymus at the intensity of 0.05 W/cm2, a single exposure of 2 minutes, at the rate of 6 treatments every 1-2 days, after which they reproduced the model of immunodeficiency (see gr) and after 5 days held control of immunological parameters.

The 10 animals of the fourth group pre-made course exposure to EHF millimeter radiation on the area of the thymus at a wavelength of 5.6 mm, a single exposure of 2 minutes, at the rate of 6 treatments every 1-2 days, after which they reproduced the model of immunodeficiency (see gr) and after 5 days held control of immunological parameters.

Studied the following parameters: the mass of the thymus in mg; lysozyme activity of blood plasma OWhen in µg/ml; indicators of phagocytosis by neutrophils - Auianou and Basukaviru: phagocytic activity of leukocytes (FAL) in % and phagocytic index (PHIL) units, the rate of completion of phagocytosis (NRF) in %; phenotypic pokazatel is - blood levels of E-ROCK Society. and E-ROCK act. on M.Jondal et al. at %, the content in the spleen antibody productive cells (AFC) N.K.Erne in CL/PE.

The obtained data testify about a particular advantage of the method of the invention before the method prototype. So, in the result of the combined effects of pathogenetic factors obtained replicable model of immunodeficiency (group 2): the weight of the thymus with 290,6±18.4 to 183,6±16,9 mg (P<0,001), level of phagocytic activity of blood 63,7±2,67 to 33.2±4,01% (P<0,001), phagocytic index of 1.66±0.16 to 0,91±0,14 (P<0.01), and NRF with 59,1±1,47 to 38.7±2,78 (P<0,001), E-ROCK Society. from 62.5±1,92 to 44.9±1,74 (P<0,001), E-ROCK act. from 50.4±2,31 to 22.3±1,15 (P<0,001), AFC in the spleen with 164,2±7,51 to 74.5±8,13 (P<0,001).

Ultrasound warned the development of most of the described disorders (see table), yielding thus EHF. So, significantly more than under the influence of ultrasound, the use of EHF before modeling immunodeficiency increased thymus weight, the content of lysozyme in the serum, blood levels of E-ROCK act. and in the spleen AOK (P<0,05-0,001). Effective in the aspect of prevention, but less demonstrative was the phagocytic indices and E-ROCK Society.

Thus, the statistical processing also confirmed the effectiveness of the developed method.

The method can be illustrated by the following is the reamers.

1. Rat male Wistar rats weighing 165 g - intact. Thymus - 305 mg, lysozyme serum - 9,35 µg/ml, FAL - 56%, PHIL - 1,05%, NRF - 63,8%, E-ROCK Society. - 63,0%, E-ROCK act. - 58,0%, KLA - 160 CL/PE.

2. Rat male Wistar rats weighing 165 g - model (geksenalovy anaesthesia + abdominoscopy + immobilization stress within 24 hours). Thymus - 205 mg, lysozyme serum - 9,35 µg/ml, FAL - 28,0%, PHIL - 0,98%, NRF - 39,9%, E-ROCK total of 51.0%, E-ROCK act. - 23,0%, KLA - 56 CL/PE.

3. Rat male Wistar rats weighing 185 g - method prototype. 6 sessions of ultrasound on the region of the thymus at the intensity of 0.05 W/cm2, single exposure 2 minutes, 6 treatments 1 (weekdays) - 2 (Saturday-Sunday) day. The next day after the last treatment - play the above model, and 5 days after this study immunological parameters. Thymus - 160 mg lysozyme serum cent to 8.85 mg/ml, FAL - 38%, PHIL - 1,55%, NRF - 42,6%, E-ROCK Society. - 52%, E-ROCK act. - 47%, KLA - 120 CL/PE.

4. Rat male Wistar rats weighing 190 g - the method of the invention. 6 sessions microwave millimeter-wave apparatus of the "Reality-1 in the region of the thymus at a wavelength of 5.6 mm, a single exposure of 2 minutes, 6 treatments 1 (weekdays) - 2 (Saturday-Sunday) day. The next day after the last treatment - play the above model, and 5 days after that - immunological study on the simple. Thymus - 353 mg, serum lysozyme and 13.3 μg/ml, FAL - 58%, PHIL - 1,02%, NRF - 50,8%, E-ROCK Society. - 60%, E-ROCK act. - 68%, KLA - 240 CL/PE.

5. Rat male Wistar rats weighing 165 g - the method of the invention. 6 sessions microwave millimeter-wave apparatus of the "Reality-1 in the region of the thymus at a wavelength of 5.6 mm, a single exposure of 1 minute, 6 treatments 1 (weekdays) - 2 (Saturday-Sunday) day. The next day after the last treatment - play the above model, and 5 days after this study immunological parameters. Thymus - 358 mg, lysozyme serum - 14,38 µg/ml, FAL - 58%, PHIL e 2.06%, NRF - 45,2%, E-ROCK Society. - 62%, E-ROCK act. - 59%, KLA - 189 CL/PE.

6. Rat male Wistar rats weighing 195 g - the method of the invention. 6 sessions microwave millimeter-wave apparatus of the "Reality-1 in the region of the thymus at a wavelength of 5.6 mm, a single exposure 3 minutes (longer edge), 6 procedures 1 (weekdays) - 2 (Saturday-Sunday) day. The next day after the last treatment - play the above model, and 5 days after this study immunological parameters. Thymus - 198 mg, lysozyme serum - 10,63 µg/ml, FAL - 52%, PHIL - 1,21%, NRF - 45,8%, E-ROCK Society. - 54%, E-ROCK act. - 58%, KLA - 156 CL/PE.

7. Rat male Wistar rats weighing 180 g - the method of the invention. 6 sessions microwave millimeter-wave apparatus of the "True-1" area of the thymus at a wavelength of 5.6 mm, a single exposure of 30 seconds (lower boundary), 6 procedures 1 (weekdays) - 2 (Saturday-Sunday) day. The next day after the last treatment - play the above model, and 5 days after this study immunological parameters. Thymus - 255 mg, lysozyme serum - 10,35 µg/ml, FAL - 45%, PHIL - 1,38%, NRF -62,1%, E-ROCK Society. - 42%, E-ROCK act. - 23%, KLA - 96 CL/PE.

The above illustrations indicate the advantage of the method of the invention before the method prototype, and the fact that the optimal single exposure time of application IIM 5.6 mm for the prevention of immunodeficiency is in animals 1-2 minutes.

Method of prevention of immunodeficiency in the experiment, including the impact on the region of the thymus physical factor, wherein the exposure is carried out by irradiation of the specified area microwave millimeter-wave with a wavelength of 5.6 mm, a single exposure 1-2 min when the total number of procedures 6 through one or two days, then on the next day after completion of procedures reproduce the model of immunodeficiency, through a combination of geksenalovy anesthesia, abdominator and subsequent immobilization stress, with monitoring of immunological parameters after 5 days after playback of the model.



 

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