A method of evaluating the effectiveness of pregravid preparing women for pregnancy with miscarriage hormonal origins in history

 

(57) Abstract:

In the method of conducting quantitative determination of endometrial protein - alpha-2 microglobulin fertility (AMGF) in the flushing of the uterus and menstrual flow and progesterone before and after treatment, and to increase the level of all indicators assess the training as effective. The method allows to determine performance criteria pregravid training in women with miscarriage hormonal origins in history for planning a subsequent pregnancy with minimal risk of miscarriage. 1 PL.

The present invention relates to medicine, in particular to obstetrics and gynecology, and may find application in offices miscarriage, pregnancy planning outpatient departments, engaged in specialized technique on the problem of miscarriage.

There is a method of evaluating the effectiveness pregravid training women endocrine Genesis, based on the results of hormonal research, in particular progesterone, which is the normal concentration of progesterone characterizes the usefulness of the luteal phase of the cycle (Ssilaetsja its relatively low accuracy, related to the fact that the content of progesterone does not characterize the functional usefulness of the endometrium, which is extremely important for normal pregnancy.

The objective of the invention is to improve the accuracy of determining the effectiveness of pregravid therapy in women with miscarriage of hormonal origin.

This object is achieved by the method lies in the fact that determine the content of endometrial protein alpha-2 microglobulin fertility (AMGF) in the flushing of the uterine cavity on 22-24 day cycle and menstrual secretions in this series.

Levels of AMGF, components in the flushing of the uterine cavity 11787,5±4690,8 ng/ml, menstrual flow - 49287,5±5377,7 ng/ml, which proves the effectiveness of pregravid training of women and the possibility of pregnancy. The content of AMGP in the flushing of the uterine cavity within 4655,4±2827,3 ng/ml and in menstrual flow - 10389,2±4586,3 ng/ml indicates the unpreparedness of the woman's body for the upcoming pregnancy, about the suppression belauxinteziruushih functions endometrial glands with inferiority secretory transformation of the endometrium.

To evaluate the information content And the Finance 125 women with NB before and after treatment (see table).

As can be seen from the presented data, the increase in the content of AMGP in the flushing of the uterine cavity 6-8 times in the menstrual flow is 4-6 times compared to the original is accompanied by a normalization of the level of progesterone and is a reliable criterion for the effectiveness of pregravid training women with UXO.

The following examples illustrate the method according to the invention.

Example 1.

Ill C. C. C. 31 years have complained about the miscarriage. When conducting clinical, hormonal, ultrazvukovoj and morphological examination diagnosed the failure luteal phase of the cycle (NLF). The level of AMGP in the flushing of the uterine cavity 24 day cycle was 3850 ng/ml, menstrual flow 10000 ng/ml.

After hormonal therapy, including correction of the second phase of the cycle the purpose of human chorionic gonadotropin at a dose of 1000 IU a day intramuscularly with 16 for 25 day of a cycle, progesterone levels consistent with normal values 80 nmol/l (normal range 22-80 nmol/l) and the level of AMGP in the flushing of the uterus increased to 27000 ng/ml and in menstrual flow up to 40,000 ng/ml, which allowed to conclude about the effectiveness of pregravid under the DAMI.

Example 2.

Patient D. C. Y. 24 years complained about the miscarriage. In the examination diagnosed NLF and the inferiority of the morphofunctional state of the endometrium. The level of AMGP in the flushing of the uterine cavity was 3520 ng/ml, menstrual flow - 8900 ng/ml Serum level of progesterone was 18 nmol/L.

After conducting a comprehensive hormonal, metabolic therapy, enzyme therapy, physiotherapy (helium-neon laser level AMGF increased flushing of the uterine cavity to 27900 ng/ml, menstrual flow to 41400 ng/ml When radioimmunological study, the progesterone level increased to 76 nmol/l, which testifies to the effectiveness of pregravid training.

Example 3.

Patient K. O. T. 26 years old, came about miscarriage. In the examination diagnosed NLF and the inferiority of the functional state of the endometrium. The level of AMGP in the flushing of the uterine cavity was 2540 ng/ml, menstrual flow - 7600 ng/ml Serum level of progesterone was normal (69 nmol/l).

A pregnancy ended in spontaneous interruption in the period of 8 weeks. This item is ittok with miscarriage hormonal origins in history. The accuracy of the method amounted to 87.6%.

A method of evaluating the effectiveness of pregravid preparing women for pregnancy with miscarriage hormonal origins in history, including determining the level of progesterone, characterized in that it further determine endometrial protein alpha-2 microglobulin fertility (AMGF) in the flushing of the uterine cavity on 22-24-day cycle and menstrual secretions before and after treatment and the increase in runoff from the uterine cavity is more than 6 times in the menstrual discharge more than 4 times and with increasing levels of progesterone assess the training as effective.



 

Same patents:
The invention relates to medicine, in particular to ophthalmology
The invention relates to medicine, namely to methods of diagnosis
The invention relates to medicine and can be used in the selection of adjuvant therapy in disseminated peritonitis

The invention relates to medicine, namely to cardiology
The invention relates to medicine, namely to gynecology, and can be used for objective assessment of exacerbation and severity of chronic varicose veins ovary /varicocele/

The invention relates to medicine, namely to Oncology and clinical biochemistry

The invention relates to experimental medicine and Mineralogy, in particular to the field of research biomineral structures, and particularly to a method of determining the content of protein compounds in kidney stones
The invention relates to medicine, namely to obstetrics
The invention relates to medicine, namely to Oncology, surgery, laboratory diagnosis, and can be used to predict the possibility of radical or conditionally radical surgery for gastric cancer

FIELD: medicine, ophthalmology.

SUBSTANCE: in lacrimal liquid one should detect the content of interleukin 8 (IL-8) and that of interleukin 1 beta (IL-1β) to calculate prognostic coefficient (PC) due to dividing the first value by the second one by the following formula: At PC value being below 10.0 one should predict favorable disease flow, and at PC value being above 10.0 - unfavorable flow.

EFFECT: higher accuracy of prediction.

2 ex

FIELD: forensic medicine.

SUBSTANCE: for the purpose to detect the sequence of applied lesions at availability of several wounds, scratches and ecchymoses on a cadaver one should study the activity of alkaline peptides isolated out of affected tissue by the impact of blood neutrophils of healthy donors upon phagocytosis. Moreover, the highest stimulating effect belongs to the peptides isolated out of the lesion applied earlier. The method enables to detect the sequence of applied lesions more accurately and differentiate the repeated lesion applied 5 min later, or more.

EFFECT: higher efficiency and accuracy of detection.

2 ex, 2 tbl

FIELD: medicine, biochemistry.

SUBSTANCE: in blood serum one should detect the level of lactoferrin and biliary acids. At their ratio being equal to 5-17 it is necessary to detect chronic hepatitis of high activity.

EFFECT: higher accuracy of detection.

3 ex

FIELD: medicine.

SUBSTANCE: method involves determining cathepsin D activity in endometrium bioptate. The value being equal to or less than 0.1 units of enzymatic activity per hour, external genital endometriosis is diagnosed.

EFFECT: high accuracy of diagnosis.

1 tbl

FIELD: medicine.

SUBSTANCE: method involves studying lactoferrin content in blood serum and peritoneal exudates in postoperative period every day during the first three days. Lactoferrin concentration in blood serum being concurrently reduced by 0.02 mcmole/l or less and increasing lactoferrin concentration in peritoneal exudates by 0.04 mcmole/l or more, enteric detoxication is considered to be effective.

EFFECT: high quality of estimation.

2 tbl

FIELD: medicine.

SUBSTANCE: method involves determining plasminogen/plasmin, α2-macro-globulin, α1-antitripsin content at the first, third, fifth and tenth day. The plasminogen/plasmin level being equal to 66-74 mcmole/l or 100-120 mcmole/l, α2-macro-globulin level of 2.7-3.0 mcmole/l, α1-antitripsin content of 2.38-3.2 mcmole/l, systemic inflammatory response to purulent infection, light severity degree endotoxicosis is diagnosed and favorable disease outcome is predicted. The plasminogen/plasmin level being equal to 50-65 mcmole/l or 125-160 mcmole/l, α2-macro-globulin level of 2.3-2.6 mcmole/l, α1-antitripsin content of 3.3-4.0 mcmole/l, sepsis with organ and system dysfunction, moderate severity degree endotoxicosis is diagnosed and septic complication availability and lingering disease development course is predicted. The plasminogen/plasmin level being equal to 39-40 mcmole/l, α2-macro-globulin level of 1.58-2.08 mcmole/l, α1-antitripsin content of 5.0-6.2 mcmole/l, severe sepsis, septic shock, severe degree endotoxicosis is diagnosed and unfavorable disease outcome is predicted.

EFFECT: high accuracy of diagnosis.

5 tbl

FIELD: medicine, biochemistry.

SUBSTANCE: at testing one should precipitate high-molecular compounds with acetonitrile and register supernatant's spectral characteristics. Supernatant should be applied onto a paper filter, dried and put into solution containing aromatic aldehyde, acetone and concentrated hydrochloric acid taken at weight ratio of 70:5:1 to be kept for 2-3 min. Then it should be once again dried up to detect qualitative and semiquantitative content of oxidized tryptophan metabolites by intensity and chromatic shades. Moreover, by chromatic shades of yellow dyeing it is possible to detect the content of hydroxylated metabolites and by chromatic shades of violet dyeing - that of unhydroxylated ones.

EFFECT: higher significance of detection.

3 ex

FIELD: medicine, anesthesiology, resuscitation.

SUBSTANCE: in patients one should study the content of lactoferrin in peritoneal exudates during the 1st d of postoperational period and at decreased value being below 3500 ng/ml on should predict unfavorable result. The suggested method provides correction of possible postoperational complications that deteriorate the flow of peritonitis and lead to lethal result.

EFFECT: higher accuracy of prediction.

3 ex

FIELD: veterinary medicine.

SUBSTANCE: method involves determining low and middle molecular mass substances content in blood plasma and erythrocytes and general blood plasma albumin concentration. Integral index is calculated on basis of obtained values using formula II=100*S238-298(plasma)/S238-298(erythrocytes)*GAC, where S238-298(plasma) and S238-298(erythrocytes) are the low and middle molecular mass substances content in blood plasma and erythrocytes, respectively, determined from area of figures restricted by spectral curves in wavelength range of 238-298 nm and abscissa axis (conditional units2); GAC is the general blood plasma albumin concentration (g/l). The value being from 2.1 to 3.0, the first endotoxicosis degree is diagnosed. The value being from 3.1 to 4.5, the second endotoxicosis degree is diagnosed. The value being from 4.5 to 6.0, the third endotoxicosis degree is diagnosed. The value being greater than 6.0, the fourth endotoxicosis degree is diagnosed. The normal value is equal to 0.5-2.0.

EFFECT: high accuracy of diagnosis.

1 dwg, 1 tbl

FIELD: medicine.

SUBSTANCE: method involves separating blood serum proteins into fractions, determining albumins and alpha-2-globulins content and controlling their content changes during the disease development process. Gamma-globulin content is determined in per cent ratio with respect to total protein quantity. Then, changes in the fractions content are controlled from the first to the third week. Albumin content being in norm and alpha-2-globulins content becoming greater to the end of the first week by 30-50% when compared to normal value and dropping to norm at the second week end and gamma-globulin content increasing from norm by 10-30% to the second or the third week, high inflammatory process activity is to be diagnosed. Albumin content dropping by 10-30% from normal value at the second week, alpha-2-globulins content growing by 10-20% of norm and gamma-globulin content dropping by 30-50% at the second or the third week when compared to norm, low inflammatory process activity is to be diagnosed.

EFFECT: high accuracy and reliability of diagnosis.

Up!