The method of determining the direction of transport and metabolic rate between mineralized tissue and contacting the biological fluid |
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IPC classes for russian patent The method of determining the direction of transport and metabolic rate between mineralized tissue and contacting the biological fluid (RU 2242007):
     
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Invention relates to method for detection of typical marker foreign DNA sequences, usable in plant modification, in transgenic plant materials and products made of the same. Claimed identification method includes carrying out of multiplex polymerase chain reaction on the base of DNA isolated from tested material followed by hybridization of fluorescent labeled reaction products on specialized bioarrays. Hybridization data are registered by control and program complex and identification of foreign genes and regulatory zones thereof in tested material is carried out, and obtained results are quantitatively interpreted.
Method for detecting toxic action of oral mucosal interferonotherapy / 2288474
One should daily introduce into oral cavity of inbred mouse BALB/c for about 3-5 d an olive-shaped melted edge of capron fish line of 0.5-2.0 mm diameter impregnated with glycerol-containing preparation of recombinant interferon-α, containing 104-106 IU/ml recombinant human interferon-α, then in a dead mouse it is necessary to determine against an intact mouse the morphofunctional state of hepatocytes, enterocytes, lymphoid tissue of regional lymph nodes to evaluate: the absence of toxic action of oral mucosal interferonotherapy in case of no degenerative alterations in hepatocytes, enterocytes, lymphoid tissue of regional lymph nodes in inbred mouse BALB/c subjected to oral mucosal interferonotherapy; the presence of toxic action of oral mucosal interferonotherapy at availability of degenerative alterations of hepatocytes and/or enterocytes, and/or lymphoid tissue of regional lymph nodes in inbred mouse BALB/c after oral mucosal interferonotherapy. The innovation increases information value of the method suggested.
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 Method of multianalytic immune assay with using microparticles / 2379691
Invention refers to biology and medicine, namely to immunodiagnosis. There is offered method of multianalytic immune assay based on immunochemical, genetic and other types of reactions of biospecific binding analyte and ligands. There are mixed various categories of microparticles coated with biospecific reagents for binding of various required analytes and marked with one or more fluorochromes in various concentrations emitting a long-living fluorescence. The analysed sample and biospecific developing reagent marked with a detecting fluorochrome with a short-living fluorescence with its excitation area being outside that of fluorochromes with long-living fluorescence are added to the particle mixture. It is followed with reaction for biospecific complex formation. The prepared biospecific complexes are deposited on a solid-phase carrier. The fluorescence emission of all fluorochromes is excited with emitters in two spectral ranges herewith measuring an amount of long-living fluorescence in a time resolution mode to identify the microparticle and an amount of short-living fluorescence of detecting fluorochrome for measuring concentration of required analytes. Thus the concentration ratio of long fluorescing fluorochromes in microparticles for detecting the same type of analyte is constant, and for determining different types of analytes, the concentration ratio differs at least twice.
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Device comprises a measuring cavity for receiving and introducing a fluid sample. The measuring cavity has a set fixed thickness not exceeding 170 micrometres. The measuring cavity has a section fit for acquisition of its image. Within the measuring cavity, there is a dry reagent. The reagent contains as a component, a molecule conjugate with phosphor used for binding with biological components and with all other reacting components. The reacting components are soluble and/or suspended in the fluid sample. The method involves mixing of the reagent with the liquid sample to be introduced in the measuring cavity. A section of the sample in the measuring cavity is exposed to electromagnetic radiation of wavelength corresponding to wavelength of phosphor excitation. Phosphor marked biological components are detected through-thickness of the measuring cavity. Further, numerical analysis of the digital image follows to identify the biological components showing phosphor and to determine amounts of the biological components showing phosphor in the sample. The biological components are discernible on the digital image as fluorescing points emitting electromagnetic radiation of wavelength corresponding wavelength of phosphor emission.
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The invention relates to medicine and can be used in dentistry, trauma, radiology, biochemistry and pathophysiology. The method is characterized by the fact that the injected radioisotope one pair of experimental animals receiving standard feed, and the second pair of experimental animals treated with sucrose cariogenic diet, determine % of its inclusion in mineralized tissue and contacting the biological fluid in a period of time up to 24 hours, calculate the relative radioactivity of PR for each animal - OPA1, ORA2, ORA3and ORA4and when the values of PR>1, draw conclusions about the direction of transport of a substance from a biological fluid in mineralized tissue; ORA<1, draw conclusions about the direction of transport of the radioisotope in the opposite direction, and when the PR 1.0, conclude that both directions are balanced among themselves; then for these groups of experimental animals calculates the intensity of transport in the prevailing direction using the ratio of the difference ORA CUora; CUoragreater than zero indicate a +, CUoraless than zero indicate the sign ; if the values being compared Crowd have across the lagoon to the consistent fabric will be at CUorawith a large value regardless of the sign. Effect: increase the objectivity of the method. 2 Il.
The invention relates to medicine and can be used in dentistry, trauma, radiology, biochemistry, pathophysiology. There is a method of determining the state of the metabolism by calculating the % include radioactive substances in mineralized tissues and biological fluids by calculating the ratio of the number of pulses/min, detected in 1 g of tissue or 1 ml of liquid, pulse/min introduced per 1 g of body weight. [Roadside R. P. Metabolism of vitamin B1in rats of different ages contained on normal and cariogenic diet // Dentistry. - 1967. - N6. - C. 7-11. This method is chosen for the prototype. However, this method does not provide a fairly complete characterization of metabolism in mineralized tissues and biological fluids, because % inclusion does not fully reflect the state of metabolism, which is also characterized by such parameters as the prevailing direction of transport of the radioisotope between mineralized tissue and contacting the biological fluid and the intensity of transport is vannoy tissue and biological fluid and traffic in the prevailing direction may submit to the experimenters new for more information about the features of metabolism, because changes in the metabolism cannot occur without changes in the transport of metabolites, and dentists, trauma surgeons and radiologists may identify new approaches to diagnosis and treatment of fractures, malignant and benign neoplasms of bone, osteoporosis, periodontitis, dental caries and other diseases. The objective of the invention is to increase the objectivity of the method by determining the predominant direction of transport of the investigated radioisotope between mineralized tissue and contacting the biological fluid and determining the intensity of transport in the prevailing direction of the rats. This is due to the fact that one of the first pair of rats, which under certain conditions of experience, after a specified period of time after injection of the radioisotope, calculate the relative radioactivity OPA1according to the formula:
and the second rat from the first pair to calculate the OPA2but over a greater period of time after injection of the radioisotope; and another pair of rats contained in other conditions of experience in a similar time intervals after injection of the radioisotope, determine the PR3and ORA4Lozovanu fabric in the selected period of time after injection of the radioisotope; when ORA<1, draw conclusions about the prevalence of transport of the radioisotope in the opposite direction, and when the PR 1.0, conclude that both directions are balanced among themselves; then for these groups of rats determines the rate of transport in the prevailing direction using the ratio of the difference ORA CUoraby the formulas: CUor=ORA2-OPA1and CUor=ORA4ORA3; CUoragreater than zero indicate a +, CUoraless than zero indicate the sign; if the values compare CUorahave opposite signs, then the higher the intensity of the radioisotope transport of the biological fluid in mineralized tissue will be at CUorawith a large value regardless of the sign. Using this method you can determine the prevailing direction of transport of radioactive substances involved in the metabolism of the biological fluid in mineralized tissue or in the opposite direction and quantify its intensity not only between mineralized tissue and body fluids, but also between demineralizing tissue and biological fluids, and non-radioactive the IU human and animal. The method is as follows: one of the first pair of rats, which under certain conditions of experience, after a specified period of time after injection of the radioisotope, calculate the relative radioactivity OPA1according to the formula:
and the second rat from the first pair to calculate the OPA2but over a greater period of time after injection of the radioisotope; and another pair of rats contained in other conditions of experience in a similar time intervals after injection of the radioisotope, determine the PR3and ORA4; and when the values of PR>1, draw conclusions about the prevalence of transport of a substance from a biological fluid in mineralized tissue in the selected period of time after injection of the radioisotope; ORA<1, draw conclusions about the prevalence of transport of the radioisotope in the opposite direction, and when the PR 1.0, conclude that both directions are balanced among themselves; then for these groups of rats determines the rate of transport in the prevailing direction using the ratio of the difference ORA CUoraformula: KPopA1=ORA2-OPA1and CUor=ORA4ORA3; CUorabole have opposite signs, then the higher the intensity of the radioisotope transport of the biological fluid in mineralized tissue will be at CUorawith a large value regardless of the sign. Depending on the objectives of the experiment, you can conduct an unlimited number of definitions that will allow you to get a more objective assessment of metabolism in which the researcher is interested intervals. As mineralized tissue in the examples N1 and N2 used mandibular bone, and the biological fluid in contact with the bone, was blood. Example N1. Increase traffic radioisotope CUorabetween bone and blood in one prevailing direction, the two pairs of rats from 3 to 24 hours after injection of the radioisotope. Four three-month rats intraperitoneally introduced a solution of [35S]thiamin is based 10000 pulse/min per 1 g of body weight. The first pair of rats fed the standard feed vivarium (light lines - 1), from 3 to 24 hours after injection of the radioisotope decreased % inclusion in blood from 9.9 to 6.8, and the % inclusion in bone increased from 10.0 to 10.8 (Fig.1); respectively OPA1after 3 hours was 0.93, and ORA2after 24 hours - 1,58; CUori.e. raznosnom cariogenic diet (dark lines - 2), fell from 3 to 24 hours % inclusion in blood from 12.1 to 6.7, and the % inclusion in bone increased from 9.3 to 11.7. Accordingly ORA3after 3 hours was 0,79, and ORA4after 24 hours 1,74; CUor, i.e. the difference between the PR4and ORA3reached 0,95. Therefore, we can conclude that the first and second pairs of rats from 3 to 24 hours prevailed receipt of the radioisotope from the blood into the bone compared to transport in the opposite direction, and the more intense the second pair of rats contained on cariogenic sucrose diet than the first pair of rats fed the standard feed of the vivarium, as evidenced by almost doubling the size of CUorthan CUor. Example N2. The change in intensity of the transport of radioactive cuorabetween bone and blood in opposite directions in the two pairs of rats from 6 to 24 hours after injection of the radioisotope. Four three-month rats intraperitoneally introduced a solution of [75S]of selenate based 20000 counts/min per 1 g of body mass (Fig.2). The third pair of rats fed standard feed vivarium (light lines - 3), with 6 to 24 hours after injection of the radioisotope almost unchanged % inclusion in blood 67,6 and 66,8 and % inclusion in bone increased from 27.2% to 56.9. Sooty between PR6and OPA5was +0,37. The fourth pair of rats contained the last 2 months 54% sucrose cariogenic diet (dark lines - 4), a slight increase % enable blood to 78.2 after 24 hours, compared to 67.6 6 hours after injection of the radioisotope, and the % inclusion in the bone, on the contrary decreased with 60,3 to 53.8 in the same period. Accordingly ORA7was 0,89, i.e. higher than 0.67 and the OPA8while CUor, i.e. the difference between the OPA8and ORA7amounted to - 0,22. Therefore, we can conclude that from 6 to 24 hours the third pair of rats kept on a standard stern of the vivarium, prevailed receipt of the radioisotope from the blood into the bone, and the fourth pair of rats receiving 2 months cariogenic sucrose diet, prevailed output of the radioisotope in the opposite direction, and the intensity of transport was higher in the third pair of rats which CUor1>8 times higher than CUor. The proposed method of determining the prevailing direction of transport and mated with him the method for determining the intensity of transport between mineralized tissues and biological fluids have a high degree of objectivity that enables you to apply the research Institute of the possibility of delivery of a radioactive substance or a stable substance, which did not exist before in the body, in mineralized tissue for the purpose of diagnosis and treatment. So, for example, quantify the temporal dynamics of PR and CRorayou can apply when selecting a radioisotope for differential diagnosis of malignant and benign neoplasms of bone, when malignant neoplasms accumulate more of the radioisotope in the area of the tumor and the metastases than benign. Obviously more suitable for diagnosis and treatment will be a radioisotope with a large CUoradominated by transport from the blood into the bone and with greater intensity of transport in the prevailing direction. Graphics ORA and CUorasignificantly demonstrative digital tables. Finding out the direction of the prevailing transport connections between the biological fluid and mineralized tissue, as well as traffic in the prevailing direction is possible even in cases where without the proposed factors must not be performed. Using this method you can determine the predominant direction of transport of radioactive substances involved in metabolism, and describe its intensity not only between mineralizacija the non-radioactive substances and substances not taking part in the metabolism, if before the introduction it was not in human or animal. Claims The method of determining the direction of transport and metabolic rate between mineralized tissue and contacting the biological fluid, characterized in that the injected radioisotope one pair of experimental animals receiving standard feed, and the second pair of experimental animals treated with sucrose cariogenic diet, determine % of its inclusion in mineralized tissue and contacting the biological fluid in a period of 24 h, calculate the relative radioactivity of PR for each animal ora1, ORA2, ORA3and ORA4by the formula
and when the values of PR>1, draw conclusions about the direction of transport of a substance from a biological fluid in mineralized tissue; ORA<1, draw conclusions about the direction of transport of the radioisotope in the opposite direction, and when ORA = 1,0 conclude that both directions are balanced among themselves; then for these groups of experimental animals calculates the intensity of transport in prevalin the sub>1and CUor=ORA4ORA3while CUoragreater than zero indicate a +, CUoraless than zero indicate the sign ; if the values compare CUorahave opposite signs, then the higher the intensity of the radioisotope transport of the biological fluid in mineralized tissue will be at CUorawith a large value regardless of the sign.
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