The method for determining the redox potential of the walls of the stomach and duodenum
The invention relates to the field of medicine. The method is characterized by the fact that through fibrogastroduodenoscopy enter active platinum needle electrode with a diameter of 0.6 mm and a length of 3 mm, which is implanted in the wall section of the investigated organ perpendicular to the plane of the mucosa to a depth of 2 mm and the value of the redox potential is determined by the potential difference arising between the active electrode and the reference electrode is not less than 1 minute, the Invention can more accurately determine the value of the redox potential.
The invention relates to medicine, namely to methods of measurement of redox potential (Eh) of the wall of the stomach and duodenum.
There is a method of determining Rho2of the gastric mucosa (Zatevakhin And. And. and others in the book. “New technologies in the treatment of gastroduodenal ulcer bleeding”. - M., 2001).
To determine Rho2in the gastric mucosa was performed polarographic study using the original endoscopic technique: active platinum electrode contact of the type described electrode with the mucous membrane of the stomach; as reference electrode was used caramelly electrode, as a recording device - polarograph.
The method has the following disadvantages:
With this method of determining the inevitable contact of the electrode and hydrochloric acid of the gastric juice and therefore the error in measurement Rho2the ground clearance of N+.
Technical difficulties while keeping a tight contact of the electrode with a given area of the mucosa when the mobility of the wall of the stomach (peristalsis, breathing, insufflate air) leads to displacement of the electrode and also to errors in measurement.
The need for calibration of the active electrode before each measurement; indirect (polarography) measure Rho2involves a distortion of the true values.
The present invention is the study of Eh in the wall of the stomach and duodenum.
The problem is solved in that a method for determining the redox potential of the walls of the stomach and duodenum, namely, that through fibrogastroduodenoscopy enter active platinum needle electrode with a diameter of 0.6 mm and dialock to a depth of 2 mm and the value of the redox potential is determined by the potential difference, arisen between the active electrode and the reference electrode is not less than 1 minutes
The study is a Desk redox potential (Eh) occurring between the measuring electrode implanted in slist-submucosal layer of the wall of the stomach (duodenum), and the reference electrode.
The measuring electrode is a platinum electrode needle with a diameter of 0.6 mm and a length of 3 mm, the Measuring electrode is connected to a recording device through a conductor length of 2000 mm and a diameter of 1 mm. Conductor, the connection of the active electrode with the conductor and the nearest to the conductor 1 mm active electrode is covered with a layer of insulator (PVC skin). The total diameter of the conductor and insulator should not exceed 2 mm.
The reference electrode is hlorserebryany, standard, factory-made. The reference electrode is placed in the left axilla of the patient, the contact of the reference electrode and the skin through gauze napkins soaked with 0.9% sodium chloride solution.
The recording device is a voltmeter with a range of value not less than -300...+300 mV, divdat in the control solution according to GOST 8.450 at a temperature of +25°+1°C.
Fibrogastroduodenoscopy is introduced into the lumen of the stomach (the duodenum), the end face of fibrogastroduodenoscopy is placed in front of the investigated area of the wall of the body at a distance of 5 mm from the last.
Through the working channel of fibrogastroduodenoscopy in the lumen of the stomach (duodenum) is entered active electrode with the conductor.
The active electrode is implanted in the surveyed area of the wall of the stomach (duodenum) perpendicular to the plane of the mucosa to a depth of 2 mm to full contact insulator with a mucous membrane.
The recording device will be switched to the definition of potential. The true value of oxidation-healing capacity is the value to be registered not less than 1 min after the start of measurement.
The proposed method allows you to set the ratio of activity of oxidants and antioxidants in the tissue by the formula
where SW - stationary oxidative-up capacity for a given temperature,
[Red] is the concentration of reducing agents in tissue,
[Ox] is the concentration of oxidants in the tissue.
When oszenia [Ox]/[Red] and, therefore, Eh reflects the activity of peroxidation processes and the viability of the antioxidant system in this tissue under these conditions.
[Oh] is observed in the presence of tissue ischemia or reperfusion syndrome with the development of tissue necrosis.
Were carried out experimental measurements:
In rats, the white line was carried out by measurement of Eh in the thigh muscles (h=+10 mV). Simulated ischemia tissue temporal compression of the aorta within 15 min, while Eh +60 mV. With the resumption of blood flow Eh(up to +20-15 mV (histologically - acute muscular dystrophy). When compression of the aorta more than 15 min was noted Eh>90 mV (histologically - tissue necrosis).
The method was tested on the basis of the Moscow hospitals.
The volunteers in the absence of pathology of the stomach and duodenum Eh=+15 mV. In acute tissue blood loss Eh +60-70 mV. Were subsequently identified acute erosive-ulcerative lesions of the wall of the stomach or duodenum that was predicted Eh.
Thus, indicators of change in Eh compared to the norm SW serves as a criterion for determining tissue necrosis.
The method for determining the redox potential is positive needle platinum electrode with a diameter of 0.6 mm and a length of 3 mm, which is implanted in the wall section of the investigated organ perpendicular to the plane of the mucosa to a depth of 2 mm and the value of the redox potential is determined by the potential difference arising between the active electrode and the reference electrode is not less than 1 minutes
FIELD: medicine, hepatology.
SUBSTANCE: one should detect the level of hepato-specific enzymes (HSE) in blood plasma, such as: urokinase (UK), histidase (HIS), fructose-1-phosphataldolase (F-1-P), serine dehydratase (L-SD), threonine dehydratase (L-TD) and products of lipid peroxidation (LP), such as: dienic conjugates (DC), malonic dialdehyde (MDA). Moreover, one should detect the state of inspecific immunity parameters, such as: immunoregulatory index (IRI) as the ratio of T-helpers and T-suppressors, circulating immune complexes (CIC). Additionally, one should evaluate the state of regional circulation by applying rheohepatography (RHG), the system of microhemocirculation with the help of conjunctival biomicroscopy (CB) to detect intravascular index (II). In case of increased UK, HIS levels up to 0.5 mcM/ml/h, F-1-P, L-SD, L-Td, LP products, CIC by 1.5 times, higher IRI up to 2 at the norm being 1.0-1.5, altered values of regional circulation, increased II up to 2 points at the norm being 1 point, not more one should diagnose light degree of process flow. At increased level of UK, HIS up to 0.75 mcM/ml/h, F-1-P, L-SD, L-TD, LP products, CIC by 1.5-2 times, increased IRI up to 2.5, altered values of regional circulation, increased II up to 3-4 points one should diagnose average degree of process flow. At increased level of UK, HIS being above 0.75 mcM/ml/h, F-1-P, L-SD, L-TD, LP products, CIC by 2 and more times, increased IRI being above 2.5, altered values of regional circulation, increased II up to 5 points and more one should diagnose severe degree of process flow.
EFFECT: higher accuracy of diagnostics.
FIELD: medicine, infectology, hepatology.
SUBSTANCE: in hepatic bioptate one should detect products of lipid peroxidation (LP), such as: dienic conjugates (DC), activity of antioxidant enzymes, such as: catalase (CAT)and superoxide dismutase (SOD). One should calculate by the following formula: C = DC/(SOD x CAT)x100, where DC - the content of dienic conjugates, SOD - activity of superoxide dismutase, CAT - activity of catalase. At coefficient (C) values being above 65 one should predict high possibility for appearance of cirrhosis, at 46-645 - moderate possibility and at 14-45 -low possibility for appearance of cirrhosis.
EFFECT: higher accuracy of prediction.
FIELD: medicine, clinical toxicology.
SUBSTANCE: at patient's hospitalization one should gather the data of clinical and laboratory values: on the type of chemical substance, patient's age, data of clinical survey and laboratory values: body temperature, the presence or absence of dysphonia, oliguria being below 30 ml/h, hemoglobinuria, erythrocytic hemolysis, exotoxic shock, glucose level in blood, fibrinogen and creatinine concentration in blood serum, general bilirubin, prothrombin index (PTI), Ph-plasma, the state of blood clotting system. The state of every sign should be evaluated in points to be then summed up and at exceeding the sum of points being above "+20" one should predict unfavorable result. At the sum of "-13" prediction should be stated upon as favorable and at "-13" up to "+20" - prediction is considered to be doubtful.
EFFECT: higher accuracy of prediction.
2 ex, 3 tbl
FIELD: medicine, juvenile clinical nephrology.
SUBSTANCE: disease duration in case of obstructive pyelonephritis should be detected by two ways: either by detecting the value of NADPH-diaphorase activity, as the marker of nitroxide synthase activity in different renal department and comparing it to established norm, or by detecting clinico-laboratory values, such as: hemoglobin, leukocytes, eosinophils, urea, beta-lipoproteides, lymphocytes, neutrophils, the level of glomerular filtration, that of canalicular reabsorption, urinary specific weight, daily excretion of oxalates, arterial pressure, and estimating their deviation against average statistical values by taking into account a child's age.
EFFECT: higher efficiency of detection.
7 dwg, 1 ex, 6 tbl
FIELD: clinical medicine, pulmonology.
SUBSTANCE: one should carry out complex estimation of interleukin-1β) concentration in blood, saliva, bronchoalveolar liquid. Moreover, one should detect distribution coefficient (DC) for IL-1β as the ratio of IL-1β blood content to IL-1β salivary content. At increased IL-1β blood content by 10 times and more, by 2 times in saliva, unchanged level of bronchoalveolar IL-1β, at DC for IL-1β being above 1.0 one should predict bronchial obstruction. The method enables to conduct diagnostics of the above-mentioned disease at its earlier stages.
EFFECT: higher efficiency of prediction.
FIELD: medicine, diagnostics.
SUBSTANCE: the present innovation deals with genetic trials, with diagnostic field of oncological diseases due to analyzing DNA by altered status of gene methylation that take part in intracellular regulation of division, differentiating, apoptosis and detoxication processes. One should measure the status of methylation in three genes: p16, E-cadherine and GSTP1 in any human biological samples taken out of blood plasma, urine, lymph nodes, tumor tissue, inter-tissue liquid, ascitic liquid, blood cells and buccal epithelium and other; one should analyze DNA in which modified genes of tumor origin or their components are present that contain defective genes, moreover, analysis should be performed due to extracting and purifying DNA out of biological samples followed by bisulfite treatment of this DNA for modifying unprotected cytosine foundations at keeping 5-methyl cytosine being a protected cytosine foundation followed by PCR assay of bisulfite-treated and bisulfite-untreated genes under investigation and at detecting alterations obtained according to electrophoretic result of PCR amplificates, due to detecting the difference in the number and electrophoretic mobility of corresponding fractions at comparing with control methylated and unmethylated samples containing normal and hypermethylated forms of genes one should diagnose oncological diseases. The method provides higher reliability in detecting tumors, detection of remained tumor cells after operation.
EFFECT: higher efficiency of therapy.
1 cl, 3 dwg, 4 ex
FIELD: medicine, gastroenterology.
SUBSTANCE: one should carry out diagnostic studying, moreover, on the 5th -6th d against the onset of exacerbation in case of gastric and duodenal ulcerous disease one should detect the content serotonin, histamine and acetylcholine in blood, then during 2-3 wk one should conduct medicinal therapy to detect serotonin, histamine and acetylcholine level in blood again and at serotonin content being by 2-3 times above the norm, histamine - by 1.15-1.4 times above the norm and acetylcholine - by 20-45% being below the norm one should predict the flow of gastric and duodenal ulcerous disease as a non-scarring ulcer.
EFFECT: higher accuracy of prediction.
SUBSTANCE: method involves taking blood from ulnar vein (systemic blood circulation) and from large vein of the injured extremity proximal with respect to lesion focus (regional blood circulation). Spontaneous NST-test value is determined and difference is calculated in systemic and regional blood circulation as regional-to-systemic difference. The difference value is used for predicting clinical course of pyo-inflammatory disease in extremities.
EFFECT: high accuracy of diagnosis.
4 cl, 2 tbl
FIELD: medicine, gastroenterology.
SUBSTANCE: one should introduce biologically active substance, moreover, in patient's blood serum one should detect the content of acetyl choline and choline esterase activity followed by 2-h-long intragastric pH-metry at loading with biologically active substance as warm 40-45%-honey water solution at 35-40 C, and at increased content of acetyl choline being above 1.0 mM/l, choline esterase being above 0.5 mM/l/30 min and pH level being 6.0-6.9 it is possible to consider apitherapy to be useful for treating ulcerous duodenal disease.
EFFECT: higher efficiency and accuracy of detection.
FIELD: medicine, gastroenterology.
SUBSTANCE: it has been suggested a new method to detect pharmacological sensitivity to preparations as acidosuppressors. After the intake of the preparation a patient should undergo fibrogastroduodenoscopy 3 h later, then, through endoscopic catheter one should introduce 0.3%-Congo red solution intragastrically and the test is considered to be positive at keeping red color that indicates good sensitivity to the given preparation, and in case of dark-blue or black color the test is considered to be negative that indicates resistance to this preparation. The suggested innovation widens the number of diagnostic techniques of mentioned indication.
EFFECT: higher efficiency of diagnostics.