The method for determining the redox potential of the walls of the stomach and duodenum

 

(57) Abstract:

The invention relates to the field of medicine. The method is characterized by the fact that through fibrogastroduodenoscopy enter active platinum needle electrode with a diameter of 0.6 mm and a length of 3 mm, which is implanted in the wall section of the investigated organ perpendicular to the plane of the mucosa to a depth of 2 mm and the value of the redox potential is determined by the potential difference arising between the active electrode and the reference electrode is not less than 1 minute, the Invention can more accurately determine the value of the redox potential.

The invention relates to medicine, namely to methods of measurement of redox potential (Eh) of the wall of the stomach and duodenum.

There is a method of determining Rho2of the gastric mucosa (Zatevakhin And. And. and others in the book. “New technologies in the treatment of gastroduodenal ulcer bleeding”. - M., 2001).

To determine Rho2in the gastric mucosa was performed polarographic study using the original endoscopic technique: active platinum electrode contact of the type described electrode with the mucous membrane of the stomach; as reference electrode was used caramelly electrode, as a recording device - polarograph.

The method has the following disadvantages:

With this method of determining the inevitable contact of the electrode and hydrochloric acid of the gastric juice and therefore the error in measurement Rho2the ground clearance of N+.

Technical difficulties while keeping a tight contact of the electrode with a given area of the mucosa when the mobility of the wall of the stomach (peristalsis, breathing, insufflate air) leads to displacement of the electrode and also to errors in measurement.

The need for calibration of the active electrode before each measurement; indirect (polarography) measure Rho2involves a distortion of the true values.

The present invention is the study of Eh in the wall of the stomach and duodenum.

The problem is solved in that a method for determining the redox potential of the walls of the stomach and duodenum, namely, that through fibrogastroduodenoscopy enter active platinum needle electrode with a diameter of 0.6 mm and dialock to a depth of 2 mm and the value of the redox potential is determined by the potential difference, arisen between the active electrode and the reference electrode is not less than 1 minutes

The study is a Desk redox potential (Eh) occurring between the measuring electrode implanted in slist-submucosal layer of the wall of the stomach (duodenum), and the reference electrode.

The measuring electrode is a platinum electrode needle with a diameter of 0.6 mm and a length of 3 mm, the Measuring electrode is connected to a recording device through a conductor length of 2000 mm and a diameter of 1 mm. Conductor, the connection of the active electrode with the conductor and the nearest to the conductor 1 mm active electrode is covered with a layer of insulator (PVC skin). The total diameter of the conductor and insulator should not exceed 2 mm.

The reference electrode is hlorserebryany, standard, factory-made. The reference electrode is placed in the left axilla of the patient, the contact of the reference electrode and the skin through gauze napkins soaked with 0.9% sodium chloride solution.

The recording device is a voltmeter with a range of value not less than -300...+300 mV, divdat in the control solution according to GOST 8.450 at a temperature of +25°+1°C.

Fibrogastroduodenoscopy is introduced into the lumen of the stomach (the duodenum), the end face of fibrogastroduodenoscopy is placed in front of the investigated area of the wall of the body at a distance of 5 mm from the last.

Through the working channel of fibrogastroduodenoscopy in the lumen of the stomach (duodenum) is entered active electrode with the conductor.

The active electrode is implanted in the surveyed area of the wall of the stomach (duodenum) perpendicular to the plane of the mucosa to a depth of 2 mm to full contact insulator with a mucous membrane.

The recording device will be switched to the definition of potential. The true value of oxidation-healing capacity is the value to be registered not less than 1 min after the start of measurement.

The proposed method allows you to set the ratio of activity of oxidants and antioxidants in the tissue by the formula

Eh=Eo+klg[Ox]/[Red],

where SW - stationary oxidative-up capacity for a given temperature,

[Red] is the concentration of reducing agents in tissue,

[Ox] is the concentration of oxidants in the tissue.

When oszenia [Ox]/[Red] and, therefore, Eh reflects the activity of peroxidation processes and the viability of the antioxidant system in this tissue under these conditions.

[Oh] is observed in the presence of tissue ischemia or reperfusion syndrome with the development of tissue necrosis.

Were carried out experimental measurements:

In rats, the white line was carried out by measurement of Eh in the thigh muscles (h=+10 mV). Simulated ischemia tissue temporal compression of the aorta within 15 min, while Eh +60 mV. With the resumption of blood flow Eh(up to +20-15 mV (histologically - acute muscular dystrophy). When compression of the aorta more than 15 min was noted Eh>90 mV (histologically - tissue necrosis).

The method was tested on the basis of the Moscow hospitals.

The volunteers in the absence of pathology of the stomach and duodenum Eh=+15 mV. In acute tissue blood loss Eh +60-70 mV. Were subsequently identified acute erosive-ulcerative lesions of the wall of the stomach or duodenum that was predicted Eh.

Thus, indicators of change in Eh compared to the norm SW serves as a criterion for determining tissue necrosis.

The method for determining the redox potential is positive needle platinum electrode with a diameter of 0.6 mm and a length of 3 mm, which is implanted in the wall section of the investigated organ perpendicular to the plane of the mucosa to a depth of 2 mm and the value of the redox potential is determined by the potential difference arising between the active electrode and the reference electrode is not less than 1 minutes



 

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