The method of producing bio-fertilizers

 

(57) Abstract:

The invention relates to agriculture and can be used in the manufacture of various types of granulated mineral fertilizers (ammonium nitrate, required for plant growth, urea, ammofoska and others) for crop production. The method of producing bio-fertilizers is that on granular fertilizers applied biological product on the basis of the strain Bacillus subtilis H-13”, which has antagonistic properties to phytopathogenic microorganisms and growth promoting action. As such biological product, can be used any drug on the basis of the bacterial strain Bacillus subtilis H-13” in liquid or solid fine form with a titer of not less than 106cells, the Method allows to increase the efficiency of plant protection from infectious diseases and increase crop yields. 3 C.p. f-crystals, 5 PL.

The invention relates to agriculture and can be used in the manufacture of various types of granulated mineral fertilizers (ammonium nitrate, required for plant growth, urea, ammofoska and others) for crop production.

Modern industrial production of mineral fertilizers onological the analyses of samples of different types of mineral fertilizers were received data about indigenous microflora, living on the feedstock and subsequently settling on the surface of granules of mineral fertilizers at the final stage of their production or in their further storage and transportation. The main representatives of the indigenous microflora were strains of bacteria of the genus Bacillus. Species diversity was insignificant and was represented mainly by species B. subtilis, B. cereus, B. B. megaterium. Some of the types of compound fertilizers containing phosphorus were found mushrooms. Currently there are a number of microbiological preparations for agriculture for various purposes - growth, suppress the development of pathogenic bacteria and fungi and contributing to the fixation of molecular nitrogen.

The famous “bacterial Strain Bacillus subtilis N 11 (ARRIAM), which has antagonistic and stimulating properties and how to use it to protect barley from Helminthosporium” RF patent No. 93001490 priority from 01.11.93 author Popova J. P.

The bacterial strain Bacillus subtilis No. 11, selected from a sample of soil taken from under cotton in Egypt, has antagonistic properties against phytopathogenic soil microorganisms, in particular Helmin-thosporium the step by simultaneously with the inoculation of the seeds with soil green fertilizer (green manure).

Also known Bacterial fertilizer “NICK” and the method of obtaining a bacterial fertilizers for application No. 99100664 priority from 21.01.99 authors Vinogradova E. J., Vinogradov A. E.

Granular bacterial fertilizer contains a bacterial culture of the strain Bacillus mucilaginosus (VCMB 1451D), the sorbent and the remains of the culture fluid with metabolites, in the following ratio of ingredients per 1 g of the substance:

1-100 million cells of Bacillus mucilaginosus;

0,2-0,4 amendment;

the rest remains of the culture fluid with water and metabolites.

At the same time as a sorbent of this fertilizer may contain pulverized plant mass, peat, vermiculite.

A method of obtaining a bacterial fertilizers on the basis of the strain Bacillus mucilaginosus includes the cultivation of the specified strain on liquid nutrient media-hydrolysates waste industry and agriculture, after which the resulting product is mixed with sorbent and granularit.

In the particular case of the cultivation is carried out using as a nutrient substrate an aqueous extract of bird droppings with the addition of 0.5% molasses at pH 7.5±0.1 and a temperature of 36-38° what ioricet 20.04.90 authors Vasyuchenko I. K., Perebityy A. N., Pigulevskaya B. C., Berezko M. and Puchko Century. And.

The invention relates to methods for producing substrates for growing seedlings and vegetable crops using biomass of bacteria of the genus Pseudomonas inhibitors development of phytopathogenic microorganisms.

In this way we obtain a substrate by depositing peat, lime, mineral and bacterial fertilizers with subsequent mixing of the components, as well as bacterial fertilizer use biomass of the bacterial strain Pseudomonas putida fluorescens CMPM IN-3481.

As the prototype was chosen as the Method of producing biofertilizer” RF patent No. 2130005 priority from 06.04.1996 authors Rahmanova I. T., Alimova FK, Ozhiganovo, U., Khabibullina R. E., N. Krylova.And., Fattakhova A. N.

The method consists in the fact that the strain or community of microorganisms cultivated on sterile nutrient medium to achieve a titer of bacterial mass 108-109cells/ml of the resulting biomass is separated from the environment, concentrate. The concentrated biomass is applied on the dried granulated chicken manure. Immobilized thus biomass is dried. This method ensures that the increased survival of micro the Oia when introduced into the soil provide the elements of mineral nutrition not only plants, but, and soil, including phytopathogenic microorganisms, resulting in their number in the soil can significantly increase.

There are currently no methods for production of mineral fertilizers, which could inhibit the growth and development of pathogenic microflora in the soil.

The task of the invention is to increase the efficiency of plant protection from infectious diseases associated with microbial processes in soil due to application of mineral fertilizers.

This problem is solved due to the fact that the granulated mineral fertilizers cause bacterial preparation on the basis of the strain Bacillus subtilis H-13”, which has antagonistic properties to phytopathogenic microorganisms and growth promoting action.

As such bacterial product can be used any drug on the basis of the bacterial strain Bacillus subtilis H-13” in liquid or solid fine form with a titer strain of not less than 106cells/g, as well as non-toxic to crops additives.

The strain of Bacillus subtilis H-13” deposited under registration number ARRIAM D-606 dated September 10, 1998 in the group Epiphany the loss of population (of the title) goes in the spore state, and it allows to use it as a bacterial additive to the granules of various types of mineral fertilizers used in crop production.

It was established experimentally that the bacterial preparation on the basis of the strain Bacillus subtilis H-13 has:

- high antagonistic activity against a broad spectrum of phytopathogenic bacteria and fungi;

- stimulating effect on plant growth;

- stable resistance to high concentrations of salts and other chemical components used in industrial production of granulated mineral fertilizers;

- ability to survive and to retain their useful properties in a wide temperature range from -20 to +90°C;

- ability to exist in both aerobic and anaerobic environment;

- ability to survive and exist in a wide range of pH from 3 to 11.

Thus, the combination of granulated mineral fertilizers with bacterial preparation on the basis of the strain Bacillus subtilis H-13” contributes to the protection of mineral elements from using them as a power source of phytopathogenic microflora. However, experimental the mA Bacillus subtilis H-13” not less than 106cells/year

Moreover, the observed effect of inhibiting the growth and development of pathogenic microflora, wintering and persistent in the soil on crop residues.

This is confirmed by the experimental data presented below in tables 1 and 2.

The results of the experiments are shown in tables 1 and 2 show a zone of inhibition (suppression) of phytopathogenic bacteria and fungi (control these zones are absent), then there is clearly prove the efficiency of the bacterial preparation on the basis of the strain Bacillus subtilis H-13”, combined with granulated mineral fertilizers, as an antagonist of phytopathogenic microflora.

In addition, the experimentally determined decrease in the rate of solubility of fertilizer granules, combined with bacterial drug, compared with granules of raw mineral fertilizers. For example, the following results are obtained for the solubility (%) of some granules of mineral fertilizers on agar in Petri dishes, which are shown in table 3.

The results of the experiment are shown in table 3, indicate that the mineral elements (more than just this is the flax physico-chemical condition, less soluble and therefore more resistant to the process of denitrification (the transition to a gaseous state). Thus, the enrichment of fertilizer granules with a biological product on the basis of the strain Bacillus subtilis H-13” contributes to a more efficient absorption by plants of mineral substances in the composition of these fertilizers.

In addition to the effect of the reduced solubility of fertilizer granules, combined with bacterial drug, there is the effect of intensive growth of the strain Bacillus subtilis H-13” in the granules and in the surrounding area (D=15÷30 cm.

This fact suggests that in this case the applied useful microflora using mineral nitrogen to build their bodies, fixes it in the soil and makes it more available for plant nutrition.

Experimental evidence for the efficiency of the bacterial preparation on the basis of the strain Bacillus subtilis H-13” (titer not less than 106cells/g), combined with granulated mineral fertilizers, as growth funds are shown in tables 4 and 5.

Thus, the processing of granulated mineral fertilizers bacterial preparation on the basis of strain B is Biologicheskie processes in the soil, caused by the application of mineral fertilizers, and increase crop yields.

In the particular case performance of the proposed method bacterial preparation on the basis of the strain Bacillus subtilis H-13 is used in the form of a fine dry powder with a title(1÷5)·106cells/g, which is applied on the granules of mineral fertilizers in the amount of 0.4-1.5% of the amount of substance used, which is 4 to 15 kg/T. it is found Experimentally that the titer of strain in bacterial drug and its quantity per ton of fertilizer are the best.

When the industrial production of fertilizers application of the dry form of the bacterial preparation produced by the method of powder and mixing drums, where there is a cooling process and coating of fertilizer granules waterproof oil and other compositions (taly oil, dispersant NF and others).

In addition, dry bacterial drug can easily handle the granules of mineral fertilizers in the farms of the farmers immediately before the incorporation of the fertilizer into the soil. For dusting granules in farms, you can use regular economese. The minimum rate of flow of the dry drug in the farms of the farmers should be increased by 10-15%, i.e. it will be 6-18 kg per tonne of granulated mineral fertilizers.

When dusting of granulated mineral fertilizers dry bacterial drug, an additional positive effect, consisting in the protection of granules from sintering due to the high hygroscopicity of such coverage.

In another particular case, the performance of the proposed method bacterial preparation on the basis of the strain Bacillus subtilis H-13” is used in the form of liquid fraction with a title(1÷10)·108cells/g, which sprayed granules of mineral fertilizers (except ammonium nitrate) in an amount of 0.1-0.2% of the amount of substance used, that is 1÷2 l/so

It was established experimentally that the titer of strain in bacterial drug and its quantity per ton of mineral fertilizers, are optimal for the case of implementation of the method.

When this spraying of fertilizer granules bacterial preparation is carried out by using a spray of any type and in industry this operation assistance drums, in which there is a cooling process and coating of fertilizer granules waterproof oil and other compositions (taly oil, dispersant NF and others) and their mixing.

In the particular case performance of the proposed method bacterial preparation in the form of liquid fraction with a titre not less than 1·109cells/g is applied to the surface of fertilizer granules comprising a waterproof compositions at the rate of 0.01-0.02% of the amount of substance used, which is 100-200 ml/so

In this case, the activation liquid forms of the bacterial preparation process of industrial production of mineral fertilizers. When investigating the possibility of combining the bacterial preparation on the basis of the strain Bacillus subtilis H-13” with the substances used in the process as agents that reduce the hygroscopicity of the granules (taly oil, dispersant NF and so on), it was experimentally found that the liquid form of the bacterial preparation should be made in the compositions in amounts of 5-10% of the volume of the used substance agent, with titre not less than 1·109cells/G. Thus, the flow rate of the liquid form of the bacterial preparation is 1 is the most suitable for implementation in industry, as it does not require material expenditures for additional equipment in the technological chain of production of mineral fertilizers.

Example 1.

The culture is grown bacteria Bacillus subtilis H-13” in a sterile liquid nutrient medium containing 4% wheat bran and 0.3% of chalk, on a rocking chair or in the fermenter when 37±1°C for 24-36 hours. In the culture fluid accumulate 5·108cells/ml Then get bacterial preparation in the following way. First mix:

70 ml of the culture fluid,

230 g of defecation,

then the resulting mass is dried at 60°C for 12 hours and prepare from it the fine powder by grinding (the title is a little more than 106cells/g).

Granular fertilizers in quantities of 1 ton and bacterial product in the form of fine powder in an amount of 10 kg load in a conventional mixer, where it is stirred by rotation of the drum for 5 minutes.

Received biomineral fertilizer discharged from the mixer and applied to the soil.

This method can be applied directly in the farms of farmers.

Example 2.

The grow="ptx2">Peptone 20

Glycerin 10

TO2NRA41,5

MgS41,5

on a rocking chair or in the fermenter at 37°C for 24-36 hours before reaching into the culture fluid of title 109cells/ml

Then get a liquid form of the bacterial preparation, mixing 20% of the culture fluid and 80% water (titer of bacterial drug - 2·108cells/ml).

Then 10 l of the obtained liquid bacterial drug applied to 5 tons of fertilizer granules using industry standard equipment: by spraying through a nozzle and thorough mixing of the drums, in which there is a cooling process and coating of fertilizer granules waterproof compositions.

Finished fertilizer packaged in 1-20 kg in plastic bags for sale to consumers.

Example 3.

The culture is grown bacteria Bacillus subtilis H-13” in a sterile liquid nutrient medium as described in example 2, before reaching into the culture fluid of title 1010cells/ml

Then get a liquid form of the bacterial preparation, mixing 20% of the culture fluid and 80% water (titer of bacteria drug is mixed with 10 l taly oil and sprinkle them on granular fertilizers in the amount of 5 t, as indicated in example 2.

Finished fertilizer packaged in 1-20 kg in plastic bags for sale to consumers.

1. The method of producing bio-fertilizers, including the combination of granular fertilizers on microbial biomass, characterized in that as granular fertilizer use fertilizer and biomass is used as a bacterial preparation on the basis of the strain Bacillus subtilis H-13”.

2. The method according to p. 1, characterized in that the bacterial preparation on the basis of the strain Bacillus subtilis H-13” is used in the form of a dry powder with a titer of not less than 106CL/g, which is applied to the surface of granules of mineral fertilizers based 4-15 kg/so

3. The method according to p. 1, characterized in that the bacterial preparation on the basis of the strain Bacillus subtilis H-13” is used in the form of liquid fraction with a titer of not less than 108cells/ml, which is applied to the surface of granules of mineral fertilizers based 1-2 l/t by fine spraying.

4. The method according to p. 1, characterized in that the bacterial preparation on the basis of the strain Bacillus subtilis H-13” is used in the form of liquid fraction with tsytnyk compositions based 100-200 ml of a biological product for 1 t of mineral fertilizers.



 

Same patents:
The invention relates to the field of biotechnology and relates to a method of biosynthesis of cephalosporin C in liquid nutrient medium with the use of culture Acremonium chrysogenum

The invention relates to biotechnology and is a method of obtaining gamma-aminobutyric acid (GABA) using auxotrophic for L-isoleucine bacteria Escherichia coli

The invention relates to applied Microbiology and can be used for cleaning the surface of the water from spilled oil and petroleum products

The invention relates to applied Microbiology and can be used for cleaning the surface of the water from spilled oil and petroleum products

The invention relates to the field of biotechnology, and more particularly to a strain of Shigella sonnei No. 262 and can be used to produce vaccine and diagnostic products

The invention relates to the mushroom industry and can be used in biotechnology for the production of fruit bodies of mushrooms

The invention relates to the field of biotechnology, hydrolysis industry and winemaking, and is intended for the production of purified pectolytic enzyme preparation
The invention relates to the processing of the fruit before putting it into storage
The invention relates to biotechnology, namely, construction of Escherichia coli - producer cholera toxin, and can be used to create a diagnostic test systems, allowing to detect toxigenic clones of Vibrio cholerae and Escherichia coli, as well as for the design of live vaccine strains against diarrhoeal diseases caused by pathogenic strains of V. cholerae and E.
The invention relates to methods for compound fertilizer granulation using a drum granulator-dryer (BGS) and may find application in the chemical industry for the production of complex fertilizers
The invention relates to methods for complex nitrophosphate fertilizers prolonged action, widely used in agriculture
The invention relates to compositions of nitrogen-potassium fertilizers containing ammonium nitrate and a potassium component, and methods for their preparation and can be used in agriculture and chemical industry

The invention relates to organo-mineral fertilizer and lime to the way they are received from products industrial utilization and can be used in agriculture and forestry
The invention relates to methods for complex nitrophosphate fertilizers prolonged action, widely used in agriculture
The invention relates to a method for producing nitrogen-potassium fertilizer
The invention relates to a method for producing nitrogen-potassium fertilizer
The invention relates to a method for producing a complex NPK fertilizers commonly used in agriculture
The invention relates to the production of fertilizers and can be used in the technical processes of production of organic fertilizers (WMD)

FIELD: agriculture, in particular, mineral fertilizer utilization processes.

SUBSTANCE: method involves introducing into soil phosphate flour in an amount sufficient for obtaining of estimated yield, with phosphate flour being preliminarily mixed with carbamide and phosphogypsum; spreading resulted mixture over soil surface and embedding with the use of cultivators, disk harrows or surface plows. Method allows phosphate flour to be used as fertilizer on soil with neutral or close to neutral acidity.

EFFECT: increased efficiency in utilizing of phosphate flour on acid soil.

1 ex

Up!