The way to determine ovulation and device for its implementation

 

The invention relates to the field of medicine and veterinary medicine, and specifically to obstetrics and gynecology, and can be used in the diagnostic process of the physiological state of the female reproductive system, pregnancy planning or adopting measures for protection from unwanted pregnancy, and can also be used in animal husbandry when determining the period of ovulation in animals. The way to determine the fertile period include the bringing into contact of mucous secretions from the electrodes of the sensor and subsequent measurement during the menstrual cycle polar conductivity of mucus, allowing to determine the concentration of hormone in the blood and obtained the maximum value of the polar conductivity to determine the ovulation period. The measurement of the polar conductivity mucus is carried out in a pulsed mode. The device contains a sensor with electrodes, a circuit for signal processing, comprising a block selection signal and a conversion unit of the useful signal into a digital code, the digital indicator and the power source. The block selection signal includes a load resistor, two repeaters and a capacitor. The electrodes are made of chemically homogeneous the period of ovulation. 2 C. p. F.-ly, 3 ill.

The invention relates to the field of medicine and veterinary medicine, and specifically to obstetrics and gynecology, and can be used in the diagnostic process of the physiological state of the female reproductive system, pregnancy planning or adopting measures for protection from unwanted pregnancy. It can be widely used in animal husbandry when determining the period of ovulation in animals.

Currently gynecological science and practice have a wide array of methods for determining ovulation period and funds, implementing them based on different principles that formed the basis of their functioning. We can distinguish two directions, actively developing: ultrasound and hormonal monitoring.

A known method for diagnosis of ovulation, which in the dimension in biological tissues velocity of shear waves in the traveltime of the leading edge of the pulse wave between the receiving and radiating, the Flexural piezoelectric tranducers. Alternately, put the sensor on the right nipple and then the left breast, record the time of propagation of transverse shear is s certificate of the USSR No. 1378812, IPC 6 AND 61 IN 5/05, 1984).

The known method for diagnosing biological ready women for childbirth, which consists in measuring the propagation velocity of shear waves in the skin of the nipple of the mammary glands, holding the pulsating vacuum stimulation of each nipple of the breast using a decompression chamber that simulates the lips and mouth of the infant, re-measuring the propagation velocity of shear waves in the skin of the right and left nipples and decreasing the velocity of propagation of shear waves (3 m/s and more diagnose biological readiness for childbirth (USSR author's certificate No. 1380726, IPC 6 And 61 In 5/05, 1985).

There is also known a method of determining ovulation by ultrasound in which pulse register index of the uterine artery on vaginal dopplerogramma, and the approach of ovulation is determined by the dynamics of changes in value recorded indicator (patent RF №2013989, IPC 6 And 61 In 5/00, 1991).

However, the known methods for acoustic diagnostics do not provide accurate prognostic data on the occurrence of ovulation, and can only be considered as a rapid-methods.

More accurate and complete diagnostic data about the occurrence of ovulation get the ovulation, consisting in bringing the electrodes into contact with mucous secretions of the body, which use saliva or cervical mucus, and the measurement during the menstrual cycle difference of redox potentials between electrodes made of dissimilar chemical composition of materials, and the determination of the fertile period by the maximum value of the measured parameter (RF patent No. 2128943, IPC 6 And 61 In 5/05, 1999).

This method is compared with the above-mentioned acoustic diagnostic methods greatly improve the accuracy of the designated period of ovulation, however, are needed for the implementation stationary conditions and appropriate instrumentation.

Closest to the claimed invention in part of the way to the technical essence and the achieved when using the result is a method for determining ovulation period, comprising bringing into contact of the electrodes with mucous secretions and subsequent measurement during the menstrual cycle polar conductivity of mucus in an inhomogeneous electric field, which allows to indirectly determine the concentration of luteinizing hormone in the blood, and received the 10/00, 2001).

In the known method eliminated for the most part the drawbacks of the above technical solutions, representing the prior art, thereby improving the accuracy of determining ovulation period up to several hours. It is known that this method is based on the change in potassium-sodium balance in the side of potassium, the concentration of which in the mucous secretions of the body increases by at least an order as early onset of ovulation. This changes the fine structure of the amino acids of the protein of mucus that is fixed by changing polar conductivity of mucus. The magnitude of the polar conductivity is not constant and changes over time, i.e. it is a random variable. The uncertainty of the moment of determination of the conductivity in mucous secretions in every act of measurement and is one of the major disadvantages of this method. In addition, prolonged exposure to the electric field leads to significant changes in the fine structure of the amino acids of the protein of mucus, and the measured value of the conductivity in this case carries no information about the state of the fine structure of the protein, which ultimately introduces a certain error in dostovernom electrodes, made of different metals, connected to the measuring device. During operation of the device is measured electrical parameters, depending on the oxidizing or reducing properties of vaginal secretions, which determine the beginning of the period of ovulation (patent RF 2230328, IPC And 61 In 10/00, 1974). Use information only about the redox properties of vaginal discharge causes insufficient accuracy of determining the commencement of the period of ovulation.

It is also known a device for determining ovulation period, including the electrodes, mounted in a holder of dielectric material made of dissimilar chemical composition of materials and connected to the potentiometric circuit containing a comparator, one input of which is connected to the said electrodes and the output to the input of the indicator device, a second comparator input connected to the reference threshold, connected to a stable voltage source (RF patent No. 2128943, IPC 6 And 61 In 5/05, 1999). Due to the fact that to determine the ovulation period using the difference of redox potentials, increases the accuracy of determining the desired parameter. However, the accuracy of determination of the search is snasti and achieved when using the result is a device for determining ovulation period, comprising of a sensor with electrodes having distinct from each area of contact surfaces for forming a non-uniform electric field between the electrodes, the processing circuitry of the signal containing the block selection signal and a conversion unit of the useful signal, the indicator is made in the form of a digital display (patent RF №2195863, IPC 6 And 61 In 5/05 And 61 In 10/00, 2001).

The known device has allowed to eliminate in the main shortcomings of a similar technical solutions that characterize the prior art, and to increase the accuracy of determining the commencement of the period of ovulation. However, as mentioned above, the polar conductivity is a function of time and its informative value to the state of the fine structure of the protein-only a very short time. Prolonged exposure to electric fields leads to significant changes in the fine structure of the amino acids of the protein of mucus. The beginning of the process of measuring the conductivity of mucus in time in every act of measurement is uncertain. Therefore, the resulting value of the polar conductivity is a random variable, since the conductivity of mucus, as noted above, is a function of time. Thus the terrain in the accuracy of the measured parameter - ovulation period.

The problem underlying the claimed invention is to provide a method for determining ovulation period and device for its realization, free from the disadvantages inherent in the technical solutions, representing the prior art, due to the increased use of information base processes in diagnosed organisms.

Technical result achieved during the implementation of the claimed invention is to provide a technical solution with a higher accuracy and precision (up to several hours) to determine the start of ovulation.

The problem underlying the claimed invention, achieving the above-mentioned technical result is decided to part ways due to the fact that in the known method for determining ovulation period, comprising bringing into contact of mucous secretions from the electrodes of the sensor and subsequent measurement during the menstrual cycle polar conductivity of mucus, allowing you to indirectly determine the concentration of hormone in the blood and obtained the maximum value of the polar conductivity to determine the ovulation period, the measurement of the polar conductivity with the Le, formed between the electrodes of the sensor immediately after the supply voltage on the electrodes of the above-mentioned sensor.

The problem underlying the claimed invention, achieving the above-mentioned technical result is solved in part of the device due to the fact that in the known device for determining ovulation period, containing the sensor with electrodes having distinct from each area of the contact surfaces forming a non-uniform electric field, the scheme of signal processing, comprising a block selection signal and a conversion unit of the useful signal into a digital code, the output of which is connected to the input of the digital display, and a power source connected to the above elements of the device, the block selection signal includes a load resistor in series with the sensor, two repeaters, one of which detector is connected to the sensor electrodes and said resistor, and the output of the repeater is connected to the capacitor, the storage value of the voltage across the resistor, and with the input of the second repeater, the output of which is connected to the input of the conversion unit of the useful signal into a digital code, these electrodes are made the curtain schematic diagram of the device; in Fig.2 shows the dependence of the change of the voltage drop across load resistor from time to time, and Fig.3 presents the main phases of development of the follicles, the concentration of estradiol 17in blood, the concentration of lysozyme protein in vaginal mucus and readings during the “hunt”.

As noted above, it is known influence on the potassium-sodium balance of the body's hormonal and using the concentration in the blood luteinising hormone to determine the ovulation period. As a result of the authors of the studies it was found that under the influence of an electric field fine structure of the protein amino acids is quickly destroyed and the measured value of the conductivity in this case, it carries no information about the real state of the fine structure of the protein. In this regard, the proposed measurement of conductivity to carry out in a pulsed mode, you can fix the size of the polar conductivity at the time of occurrence of an inhomogeneous electric field in the sensor. I.e., in every act of measurement polar conductivity mucus start measuring the conductivity of a strictly defined in time and corresponds to the moment the supply voltage is on Alekno concentration in the blood luteinising hormone.

The method of determining ovulation period in accordance with the present invention is implemented as follows. On the surface of the electrodes of the sensor 1, which creates a non-uniform electric field in mucous secretions, apply highlighting to create an electric field in the whole volume of the investigated medium. When applying voltage to the electrodes of the sensor in mucous secretions occurs inhomogeneous electric field, which is the translational motion of the multidimensional organic chains and their partial spread. Thus, it is possible to represent the effect of a nonuniform electric field on the fine structure of the protein amino acids, resulting in polar conductivity of mucus. Note that in mucous secretions are ions. When the voltage at the electrodes of the sensor 1 pulse increases, the ionic conductivity at the initial moment more polar than that is undesirable, because there is no explicit maximum value of conductivity. Therefore, the voltage at the electrodes of the sensor 1 increases from 0 to 3 In 10-15 ISS. In this case, the ionic conductivity is the background of the polar conductivity.

In accordance with Andonovski equilibrium when more, but the mobility is higher. In the future, under the influence of the field in mucous secretions is a dynamic equilibrium with a predominance of ionic conductivity.

The method in accordance with the present invention is implemented by a device, the functional diagram is shown in Fig.1.

The device consists of a sensor 1 with the electrodes, resistance 2, two repeaters 3 and 4, one of which is the repeater 3 is detecting, condenser 5, unit conversion signal into a digital code 6, a liquid crystal digital display 7, the power source 8, a battery 9, the network adapter 10. The device is powered from its own battery 9 can be recharged from mains 220 C. the batteries provides intensive operation for several days. To recharge the batteries you are using an external network adapter 10 (Converter 220 V - 9). The power supply 8 provides the necessary power to all blocks of the device and charge the battery when connecting the external network adapter 10.

The device operates as follows. A sample of mucus taken from the test mammal inflicted with the condition of Deleni difficult or too thick, the porous material used for sampling, pre-moistened with saline solution and after sampling the contents of a porous material is pressed against the surface of the sensor.

Sensor 1 when connected to a power source 8 generates in the study mucous secretions deposited on the surface of the sensor electrode, a nonuniform electric field. For this purpose, the sensor electrodes 1 made of a chemically homogeneous material, but differing from each other by the contact surfaces, between which is formed a non-uniform electric field.

After receiving voltage from the power source to the electrodes of the sensor 1, the potential at the point a of the circuit equal to the voltage drop across the resistor 2. The time dependence of the voltage (potential) at the point a (Ua) is shown in Fig.2. The voltage Ua across the buffer cascade detector repeater 3) is supplied to the condenser 5, which is charged to the value of Ua. Resistance input buffer stage (repeater 4) and output buffer stage (detection repeater 3) is so great, that allow to maintain the potential on the capacitor 5 unchanged for a long time. During this time, the operator fixes bocasana 6, continuously producing a signal processing from the output of the repeater 4. Thus, it provides a measurement value proportional to the maximum polar conductivity in this act of measurement.

The present invention was tested in several regions of the Russian Federation. So Strazilovska stud in accordance with the present invention were covered 240 mares. At the same time these mares were taken blood tests for estradiol 17. The main results of the experiment shown in Fig.3, where Fig.3A shows the relative main phases of development of follicles in Fig.3B - concentration of estradiol 17in the blood, in Fig.3C - the concentration of total protein and lysozyme in the vaginal mucus of Fig.3D readings during the ripening of the follicles. As follows from the obtained results, the maximum change in conductivity in mucous secretions corresponds to the maximum blood concentration of estradiol 17. Therefore, a more precise determination of the achievement of this point by measuring the magnitude of the polar conductivity at a certain time will significantly improve the accuracy of determination of the period s the objective of its use in all spheres of application of the present invention.

Claims

1. The method of determining ovulation period, comprising bringing into contact of mucous secretions from the electrodes of the sensor and subsequent measurement during the menstrual cycle polar conductivity of mucus, allowing you to indirectly determine the concentration of hormone in the blood, and obtained the maximum value of the polar conductivity determine the period of ovulation, characterized in that the measurement of the polar conductivity of mucus in every act of measurement is carried out in a pulsed mode, in a non-uniform electric field formed between the electrodes of the sensor, at the time of applying voltage to the electrodes of the above-mentioned sensor.

2. A device for determining ovulation period, containing the sensor with electrodes having distinct from each area of the contact surfaces forming a non-uniform electric field, the scheme of signal processing, comprising a block selection signal and a conversion unit of the useful signal into a digital code, the output of which is connected to the input of the digital display, and a power source connected to the above elements of the device, characterized in that it block allocation helpful is the second of which is connected to the sensor electrodes and said resistor, and the output connected to the input of the second repeater, the output of which is connected to the input of the conversion unit of the useful signal into a digital code, these electrodes are made of a chemically homogeneous material.



 

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FIELD: medicine, clinical toxicology.

SUBSTANCE: at patient's hospitalization one should gather the data of clinical and laboratory values: on the type of chemical substance, patient's age, data of clinical survey and laboratory values: body temperature, the presence or absence of dysphonia, oliguria being below 30 ml/h, hemoglobinuria, erythrocytic hemolysis, exotoxic shock, glucose level in blood, fibrinogen and creatinine concentration in blood serum, general bilirubin, prothrombin index (PTI), Ph-plasma, the state of blood clotting system. The state of every sign should be evaluated in points to be then summed up and at exceeding the sum of points being above "+20" one should predict unfavorable result. At the sum of "-13" prediction should be stated upon as favorable and at "-13" up to "+20" - prediction is considered to be doubtful.

EFFECT: higher accuracy of prediction.

2 ex, 3 tbl

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