Method and device photoimmunology therapy on ghevondian and zharova

 

The invention relates to medicine and biology, in particular to physical therapy and immunology for the correction of immunodeficiency and associated diseases when exposed to light different areas of the person. The way photoimmunology therapy includes coherent and not coherent radiation natural radiation in the optical range, from x-ray to radio wave of biological objects, including nadkarni, and/or intravenous, and/or in vitro irradiation of blood and/or components, and/or external and/or interstitial endoscopic irradiation of the thymus and/or spleen, and/or mucous membranes, and/or lymph nodes, and/or vessels, with light matrices perform selective irradiation and/or spatially extended areas of the bio-object associated with the local foci of specific diseases and/or directly and/or indirectly with the Central and/or peripheral organs of the immune system, or a combined effect on the biological object in the combined exposure with other factors such as physical and/or chemical and/or pharmaceutical preparations and determination of the optimal energy and/or spectral parameters and/or doses when predvaritelnii after treatment and/or duration of the treatment cycle is performed on the basis of changes in the ratio of high - and discouvery normal antibodies in serum and/or the secrets of the biological object under test in vitro, in the treatment - before, during or after exposure in vivo, while the criterion of achievement immunocorrective effect is the increase in the content vysokoavidnyh antibodies to 75-100% while reducing discouvery antibodies to 0-25%, the criterion of achievement immunosuppressive effect is a decrease in the content Vysocany antibodies to 5-25% while increasing discouvery antibodies to 75-95%, and the optimal dose effects when treatment is determined on the basis of the transit time of all antibodies from one state of avidity in the other, respectively, when the test exposure in vitro, and also taking into account the severity of immunologic deficiency, the severity of the disease, irradiated area, and the optimal dose effects on biological object in one session are calculated according to the formula: Ec=K×T×B×S, where Ewith- the duration of the session, min, K - correction factor (depends on device), T - exposure, min required for the conversion discouvery antibodies in vysokoavidnyh status set to pre-test the effect on the serum and/or secrets of bioobject in vitro, B - the degree of severity of the disease, evaluate on a scale in which 4 - a severe form of the disease, S - area of exposure. The device immunomodulatory therapy contains optical effects connected with power supply unit and the control unit, with an additional control unit is connected with unit feedback, including units of sampling, preparation of blood serum analysis and unit adjustments made with the possibility of information signals in a software unit, and the control unit is configured to implement programmable test modes exposure to serum in vitro before treatment and immune disorders using the parameters defined during the test exposure. The unit of analysis includes a die with cells, the automatic measurement of titles and optical module in the form supplied to the individual cells of the fiber and the radiation receivers and performed as a test of radiation and reception of the effects of absorption and/or scattering in the sediments formed at the bottom of the individual cells. The use of the invention improves the efficiency of treatment of disorders of the immune system. 2 S. and 6 C.p. f-crystals, 4 tab., 4 Il.

Known methods and devices for svetaderevjanko impact on different areas of the body, including for the purpose of correcting the immune system [1-4]. They use sources of optical radiation, such as lasers or LEDs, are connected to the power supply. The radiation source is usually placed in the front-end part of the extension nozzles or connected with an optical fiber through which the radiation is directed to the biological object. It is also practised using multiple lasers or narrowband sources in the form of LEDs with wavelengths lying in the spectral range from 0.25 μm to 3 μm. The radiation sources can operate in a wide range of frequencies and duty cycle both in continuous and pulsed mode.

Analysis of the few research results on the use of such optical sources mainly based on He-Ne and semiconductor lasers for the correction of the immune system shows that, in General, optical radiation affects functionalties and proliferative activity, changes the quantitative indicators of cellular immunity: number of total and active T-lymphocytes, the ratio of subpopulations of T cells (SV/SP, the helper-suppressor ratio), stimulate macrophage activity [2-4].

Information about the change in the number of b-lymphocytes in the blood during therapy very divergent.

Usually, in experiments the radiation directed or directly on the area of pathology, or localized to region of Central and peripheral organs of the immune system, including the thymus, spleen and lymph nodes.

A similar work can serve as a method and device described in U.S. patent No. 6084242 [5]. It radiation from an infrared semiconductor laser is directed to the biological object by using a light guide in the form of a hollow tube for irradiation local pathologies of the body in the treatment of cancer, arthritis, multiple sclerosis and other diseases accompanied by changes of the immune system. The immune system serves to determine the content of T - cells and CD4/CD8.

The drawback of these methods and devices is the difficulty of simultaneous irradiation with light of the lengthy and separate zones voznikaut when complex spatial geometry of these zones or at their location, for example, from different sides of the bio-object, which is typical, in particular by irradiation with extensive abnormalities of the limbs. As a result, similar to irradiation extensive pathologies requires the sequential movement of the radiation source from one zone to another, which is time consuming and may not achieve the desired effect of different times of irradiation of different zones.

Another disadvantage is the lack of clear scientific criteria for assessing the effectiveness of treatment and selection of doses. Thus, none work reviews [1-4], including the analogue of [5], is not described reliable methods and criteria for the selection of doses, that is, they were selected purely empirically. Finally today, essentially there are no objective data to prove therapeutic efficacy of light in the treatment of immune disorders, despite encouraging clinic, the available data are scarce, fragmentary, are mixed and sometimes contradictory. Moreover, it is not possible to conduct a comparative analysis of them, because the authors used different methods of optical effects, various immunological parameters, moreover, used different doses and the immunological pokazati extensive pathological areas of complex geometry is achieved in the device, described in the patent No. 2145247 [6], which is taken as a prototype. This device is a matrix of multiple radiation sources placed on the substrate to form the working surface, similar to the form of a spatially extended pathological zone. As radiation sources is proposed to use as lasers and matrix of high-brightness LEDs.

However, this invention has several disadvantages. In the inventive device does not describe the use for the treatment of various disorders of the immune system. In this regard, is absent, as in the analogue, the method of assessment immunotropic effect of optical effects and the algorithm for choosing the optimal doses. The disadvantage of this device is the inability to simultaneously selective irradiation of several extended pathological areas or organs of the Central and peripheral immune system. The use of a single large matrix can lead to unwanted exposure of healthy non-pathological zones, which increases the overall load on the body, can reduce the positive effect and lead to unwanted side effects, for example, in the form of a General rising temperatures, increasing microcirculation throughout the body, immunosuppression.

The present invention is put we have developed a fundamentally new rapid way to assess the functional activity of the immune system, based on the determination of titers of normal antibodies in serum and secretions in the reaction of the passive haemagglutination (TPHA) differentiation of two discrete groups of antibodies with high and low avidities. Normal 80-100% of the antibodies have a high avidity. The pathology and dysfunction of the immune system increases the content discouvery antibodies to 75-95%, unable to have a protective effect [7]. The method allows to quickly (within 1-2 hours) and unambiguously diagnose the presence or absence of immunological failure.

About the severity of immunologic failure can be judged by the change in the functional and protective activity of antibodies before and after transformation discouvery antibodies in vysokoavidnyh state induced by various factors both in vivo and in vitro [8].

On the detected degree of immunodeficiency, the increased level vysokoavidnyh antibodies [9].

Using these new approaches to diagnosis and treatment of immunodeficiency States, it was the first to demonstrate in vitro experiments on the blood sera of patients with various immunodeficiency disorders, as well as on pure preparations of IgG isolated from the same sera, that the effect of optical radiation can directly affect the structure and function of antibodies, in particular discouvery antibodies, transforming them into vysokoavidnyh state [10].

Thus were obtained the first evidence for a direct effect of optical radiation on antibodies and their protective properties and an opportunity has arisen for an adequate assessment of immunotropic action of optical radiation on the body.

These data provided theoretical and practical basis for understanding and formulations of this invention.

The aim of the present invention is to develop a method photoimmunology therapy and devices for selective optical effects on the affected area, taking into account individual selection of doses and objective control effectiveness.

This goal is achieved by using light matrix is selective irradiation and/or the yamo or indirectly with the Central and/or peripheral organs of the immune system, including nadkarni, and/or intravenous, and/or in vitro irradiation of blood and/or components, and/or external and/or interstitial endoscopic irradiation of the thymus and/or spleen, and/or mucous membranes, and/or lymph nodes, and/or vessels and/or combined effects on the biological object in the combined exposure with other factors such as physical and/or chemical and/or pharmaceutical preparations.

The definition of optimal energy and/or spectral parameters and/or doses at pre-test studies and/or the effectiveness of immunomodulatory effects during and/or after treatment and/or duration of the treatment cycle is performed on the basis of changes in the ratio of high and discouvery normal antibodies in serum and/or secrets when you test the in vitro studies and in the treatment - before, during or after exposure in vivo.

The criterion of achievement immunocorrective effect is the increase in the content vysokoavidnyh antibodies to 75-100% while reducing discouvery antibodies to 0-25%.

The criterion for achieving the immunosuppressive effect is a decrease in the content vysokoavidnyh antibodies to 5-25% delaetsa based on the time of the transfer of antibodies from one state of avidity to another, accordingly, when the test exposure in vitro, and also taking into account the severity of immunologic deficiency, the severity of the disease and the irradiated area.

The optimal dose effects on biological object in one session is calculated by the formula: Ewith=To×T×B×S,

where Ewith- the duration of the session, min;

K - correction factor (depends on the device used);

T - exposure, min required for the conversion discouvery antibodies in vysokoavidnyh status set to pre-test the effect on the serum and/or secrets of bioobject in vitro;

B - the degree of severity of the disease is assessed according to the scale of points:

1 - without clinical manifestations;

2 - mild disease;

3 - average form of the severity of the disease;

4 is a severe form of the disease;

S - area of the irradiated surface.

Assess the body's response to exposure is carried out by determining the level of high - and discouvery antibodies in the serum of the patient after the first three sessions.

Evaluation of the effectiveness of immune-modulating effect and/or duration of treatment is carried out by determining the content of wisniowska impact on biological object as an additional physical factor can be used optical radiation with different wavelengths, in particular, blue and/or red and/or infrared range, and/or x-ray radiation and/or ultrasound and/or temperature, and/or the introduction of oxygen and/or changing the pH of the environment. Immunomodulatory effect is achieved due to various factors, which can be sequential in time to each other and/or simultaneous in time and/or with a time delay relative to each other, and the optimal parameters and dosage are determined based on the maximum change avidity antibodies.

Improving the effectiveness of immunomodulating effects and obtaining stable clinical remission is achieved through the combined effects on the biological object. As additional factors influence the chemical and/or drug can be used immunomodulators, and/or immunosuppressants, and/or photosensitizers, and/or preparations of immunoglobulins and/or albumin, and/or mixtures thereof, and optical exposure is carried out on a biological object to the introduction and/or after administration of drugs, and/or affect themselves preparations, respectively, prior to their introduction and/or after introduction into the biological object, and the time delay between exposure to the drug dose is determined based on the maximum change avidity antibodies.

To achieve therapeutic immunomodulatory effects using in vitro irradiation of blood plasma with subsequent reinfuse her into the bloodstream after the conversion of all discouvery antibodies in vysokoavidnyh state.

Immunomodulatory effect is implemented using a device, comprising a block with a source of optical effects, coupled with the power supply, the unit of life, is associated with the bio-object and providing the necessary conditions for carrying out therapy, the control unit connected to the unit of action and unit sustainment and additional feedback, functionally connecting the control unit and the biological object that includes interconnected blocks of the sample of blood, preparation of blood serum, the unit test is deterministic transformation of antibodies, the control unit avidity antibodies, information from which is supplied to the control unit, in which are embedded two programs with the algorithm one of which provides a test of the impact on the serum in vitro before treatment and the second mode of immune correction using the optimum parameters determined during the test exposure.

For inspection and/or block enzyme immunoassay, and/or block radioimmunoassay analysis, and the block transformation contains a source physical effects.

When the control unit avidity includes standard die with the number of cells, in particular, 8×12, the automatic measurement of titles, the optical module in the form supplied to the individual cells of optical fibers and detectors for test irradiation, and to register the effects of absorption and/or scattering in the sediments, formed on a flat and/or hemispherical bottom of the individual cells.

As the source of optical effects are used alone and/or combined in a matrix of sources of optical radiation in the form of lasers and/or LEDs, and/or lamps of different type with filters, including incandescent, hid and fluorescent lamps and/or without them, sources of x-ray and/or radio-wave and millimeter ranges and/or combinations thereof.

To achieve the immunomodulatory effect device further comprises a module in vitro exposure to blood and/or sources are placed on the patient's body nadkarni, the source unit is equipped with an optical fiber connected to the devices for endoscopic iravani for the irradiation areas of the bio-object directly or indirectly associated with elements of the immune system, responsible for the maturation of antibodies in plasma cells and/or functioning of antibodies.

Strengthening the immune-modulating effect is achieved by using a combination of sources of impact of various types, including physical and/or chemical sources connected to the control unit.

1. The selection algorithm parameters and doses to achieve the immunocorrective therapeutic effect.

The method of selection involves several steps.

Step 1. Determination of content in the blood serum of a patient discouvery antibodies and titers of all normal antibodies to gram+ and/or gram - bacteria with the use of a diagnostic test system for the rapid assessment of immune status [7]. Content discouvery antibodies to 75-90% indicates the presence of immunological failure.

Step 2. At a given wavelength and intensity of irradiation in vitro is determined by the temporary exposure time exposure required to convert all discouvery antibodies in vysokoavidnyh status in the serum of the patient.

Step 3. The computation of the radiation dose for a single session taking into account the severity of immunol is rmula: Ewith=To×T×B, where:

Ewith- the duration of the session, min;

K - correction factor (depends on the device used);

T - exposure, min required for the conversion discouvery antibodies in vysokoavidnyh condition established in the preliminary test the effect on the serum and/or secrets of bioobject in vitro;

B - the degree of disease severity is scored on a scale in points:

1 - without clinical manifestations;

2 - mild disease;

3 - average form of the severity of the disease;

4 is a severe form of the disease.

Step 4. Assess the body's response to exposure to changes in the level of high and discouvery antibodies in the serum of the patient after the first three sessions.

Step 5. Evaluation of the effectiveness of irradiation on changes in the level of high and discouvery antibodies in the serum of the patient after the 5th and forth until the 10th session.

Step 6. Termination of irradiation for achievement in the serum of the patient's level vysokoavidnyh antibodies to 75-100% and reduce discouvery antibodies to 25% or their complete disappearance.

Step 7. If necessary to increase avidity antibodies and achieve stable clinical remission teleshop the chemical or biological factors.

An example of dose-response relationships change avidity antibodies

In Fig.1 shows a typical dependence of the change of avidity antibodies as a function of time of irradiation. It is the nature of the change, close to the threshold defined by two main times: threshold time at which it starts to change the avidity and the optimal time of exposure above which does not have the sense of further exposure. This dependence is key when determining the optimal irradiation parameters as in test effects on serum samples of blood, and already irradiation of the whole body.

2. An example implementation of a device photoimmunology therapy in the treatment of patients with atopic dermatitis (AD).

2.1. The description of the device.

The geometric shape of the device: an elongated hollow cylinder with the placement of the LEDs on the inner surface facing the output Windows into the cylinder. The shape of the inner surface depends on the shape of the bio-object and may be close to cylindrical, conical and spherical or combinations thereof. An example of a cylindrical section for irradiating limbs in the treatment of atopic dermatitis.

The diameter of 200 mm, the emission on the arm of the patient. On the inner surface of the cylinder is placed led emitters quantity 240 pieces with an average distance between the individual emitters 30 mm wavelength: 0.6 microns. The capacity of each oscillator operating in the continuous mode is 2 mW, the average density of the radiation on the surface of the bio-object is about 1 mW/cm2. The LEDs are connected in series-parallel. Powered from the mains voltage of 220 V and frequency of 50 Hz. At the ends photometrical system has two rubber rings 5 cm in diameter, connected with the surface of the cylinder with three springs are placed relative to each other at an angle 120°. The arm of the patient is entered sequentially in two rubber rings and due to the spring it is fixed near the axis of the cylinder. The zone is selected, exposure is carried out using the blend on hand oilcloth with the required geometry of the open zones. The total weight of the cylinder does not exceed 1.5 kg, allowing the patient is in an upright position and easily withstand the load of the described device. One session lasts 30-60 minutes. All procedures within 5-10 5-10 days. The mode is set on the basis of individual pre-test sivagami patients.

Common to all patients HELL was itching varying degrees of severity, sleep disorder, irritability, fatigue, bad mood.

The clinical picture of patients with AD was characterized by a more symmetric prone to grouping erythematous-papular rash, lesions infiltration and lichenification, point and line excruciate, peel, finely and sredneplastichnye peeling. Cutaneous manifestations discharge chute elements 26 (74,2%) patients were localized on the face, neck, torso, flexor surfaces of the extremities. 9 (25,7%) of patients with rash had widespread diffuse character.

Relapsing course HELL with similar proceeding of clinical exacerbations were observed in 10 (28,5%) patients, progressive - 23 (65%), regression - 2 (5,7%).

Characteristic for patients HELL was the increase in the number of eosinophils in the blood correlated with the SCORAD index and disease severity (r=0,711).

The results of immunological studies have shown that the distinctive feature of patients with blood pressure in the acute stage is the increase in the content of discouvery antibodies G class to 75-88% (normal 0-20%) and reduced vysokoavidnyh to 12-25% (at a rate of 80-100%). Reducing avidity an is Oh specificity (gram+, gram-) antibodies and the severity of clinical manifestations of the disease. Between the level discouvery antibodies and the magnitude of the SCORAD index was revealed a strong correlation (r=0,94), indicating a high degree of correlation between avidities antibodies G class and clinical picture of HELL.

Characteristic of patients with AD was increased 4-6 times b-lymphocytes, i.e., to 530-688 of limp./ál of the norm - 121+23 limp./ál. On the background of increase in the number of lymphocytes in 1,5-3 times.

Along with increase of b-lymphocytes was increased approximately 1.5 times the number of T-lymphocytes, mainly due to CD8 cells.

In 70% of patients HELL was rising levels of circulating immune complexes to 65-110.E. while reducing phagocytic activity of neutrophils to 65-75% (the norm 82,7+2.3%) and the ability of neutrophils to generate radicals in response to opsonization antigens to 23.6+3,5 (normal range 40-80).

In all patients with AD were impaired DETAIL status by reducing the level of alpha - and gamma-IFN and increase in blood circulating IFN from 1.5 to 4-6 ME.

Change the parameters of the T-system of immunity is not correlated with the SCORAD index.

Thus, the most informative criteria, adequately reflecting technicist criteria for evaluation of therapeutic effects in patients with AD.

2.3. The influence of low-intensity optical radiation on clinical and immunological parameters in patients with AD.

The reaction of patients HELL on irradiation wore a biphasic. In most patients the first phase after three sessions of low-intensity irradiation was characterized by increased numbers of lymphocytes in the blood and titles of normal antibodies in 2-4 times, and the level discouvery antibodies remained unchanged (75%) or were increased to 90-95% in some cases.

The second phase of the organism's reaction developed after the 5th session of low-intensity irradiation was characterized by a tendency toward normalization of lymphocyte levels and titles of normal antibodies, increase vysokoavidnyh antibodies. The maximum increase in the content vysokoavidnyh antibodies to 95-100% was 10 session almost all patients and only 8% of the content vysokoavidnyh antibodies reached 100% at a later date (two weeks after the end of physical therapy).

10 sessions of low-intensity irradiation of patients with AD have led to the decrease in the SCORAD index from 32.8+2,04 to 22.08+1,68, while the coefficient of effectiveness of treatment was 33.3+2.48 and exceeded the traditional therapy 11% (P0,001).

As a result of nizkointensivnoi the 220+84) and an increase of CD4 cells, index IRI (of 1.03 to 1.41). Showed normalization of IFN status.

Subjectively, patients itching acquired sporadically nature, and therefore decreased irritability, normalized to fall asleep in 60% of patients HELL happened reducing the frequency of exacerbation and easier for subsequent exacerbations.

Thus, low-intensity irradiation of a limited area of the skin induces in the body of a cascade of reactions, the result of which was the correction of immunological failure, improving the barrier function of the dermis and the development of severe clinical remission HELL. Exposure effect was more pronounced in comparison with traditional therapy.

2.4. The impact of combination therapy on clinical and immunological parameters in patients with AD.

The coefficient of efficiency of treatment of patients with AD treated with combination therapy, i.e., the traditional with the addition of 10 sessions of low-intensity irradiation was 41,98+1.58% and significantly exceeded the effectiveness of traditional therapy 19,68% (p 0.001) and monotherapy - 8.7% (p 0,05). The index SCORAD decreased from 50.6+2,72 to 29.4+1,74, had a higher content of vysokoavidnyh antibodies to 93% and decreased discouvery antibodies from 76% to 7%. In addition, smart is liczenie index IRI to 1.45. Decreased the number of eosinophils with 853+11 467+97, there was normalization of INF-status. The result of the combined therapy was more than a mild case of the disease and reduce the frequency of exacerbations in 76% of patients.

Thus, it becomes obvious that AD therapy should additionally include means aimed at the normalization of the functioning of the immune system, in particular to increase the secretion vysokoavidnyh antibodies G (G1, G2, G3) of the class. Only this creates favorable conditions to reduce allergens from the outside, the increased destruction and elimination of allergens and Topunov from the body, the reduction sensitization of the organism and the normalization of the functioning of the Th2 lymphocyte subpopulations and cytokine imbalance.

The identification of previously unknown relationship between activity In the immune system, influencing the fate of incoming allergens and Topunov in the body and the development of Th2 and Ig E dependent allergic reactions opens an entirely new and effective approach for the treatment and prophylaxis of allergic and atopic diseases.

As an alternative the effects on the immune system along with our previously immunomodulators [] can be successfully impact on the host pathogenetically important parts of the immune system opens the way to increase the effectiveness of therapy, cost reduction for the treatment of AD, improve their quality of life, there is the prospect control and prevent the development of atopic reactions.

3. The block diagram of the device for photoimmunology therapy.

Is depicted in Fig.2 block diagram of the device operates as follows. Optical effects 1 generates optical radiation in a temporary mode (pulsed or continuous) provided in the power supply unit 2. As radiation sources can be used as lasers of various types, and more efficient LEDs. The life-support system 3 provides the necessary external conditions for ensuring the bio-object 4. The radiation is directed to areas of the body as affected by pathology and related organs of the human immune system. Control system 5 provides the necessary parameters of irradiation energy (power, energy) and time, determine the total dose. A key element of the proposed block diagram is the feedback system 6, which provides control over effectiveness.population the avidity of antibodies, contained in serum 8, and block adjustment 9 designed in accordance with the results of the analysis to generate information signals in the programming unit 10. In the past depending on the stage of exposure is set or the test mode to determine the individual characteristics of the bio-object 4, or optimal treatment. The block 11 is designed to implement the mode of combined treatment, which along with the optical effect is the influence of other physical and chemical factors. Particularly promising is the use of various medicinal immunomodulators, and their optimal dose, time of introduction into the biological object and the time and duration of the subsequent optical radiation is determined using the described feedback system.

4. The optical part of the control unit avidity using optical fibers or matrix of LEDs.

In Fig.3 presents fragments of different options optical system unit determining the avidity of the antibodies (Fig.1, POS. 8). In the schema And the radiation from the optical source 1 is introduced into the optical fiber 2, which was supplied to the plate 3 located in Udelny this unit performs two main functions, defined modes of action. With a relatively powerful impact is deterministic transfer of antibodies from a state with low avidity. And then there is the determination of the number of relevant antibodies in accordance with the invention. Thus can investigate the behavior of antibodies at different doses to determine the optimal mode of treatment. The second function is to determine changes in the optical properties of the fluids in the process standard reaction titration. This measured value of the transmitted light through each of the die as a function of time using the matrix of photodetectors 5, located under the bottom of individual dies. The signals from each photodetector receives in multichannel recording unit 6, where they are processed and compared. The result of this comparison, the obtained information on the amount of credits and determines the magnitude of the avidity of antibodies.

Difference scheme B is used in place of the optical fibers of the individual LEDs 1, which provide exposure of each plate. Depending on the power of LEDs, you can use either one led for each die, or a small matrix. Further, as in provokingly the recording unit 4.

5. Examples fotometricna devices for simultaneous irradiation of various agencies connected with the organs of the immune system.

In Fig.4 presents examples of schemes irradiation of the biological object. Scheme And used several of photometric for irradiation of biological object 1 in the zone of the thymus 2, one of the limbs and in the area of the spleen 3. To do this, use respectively the flat photometric 4, the cylindrical photometric 5 and flexible photometric 6. The number of photometric may be extended for an additional irradiation of the lymph nodes, zones, tonsils, clusters of lymph and blood vessels, etc.

The principal factor in the choice of the number of irradiated zones is to determine the effectiveness of exposure in each case using the previously described feedback system and control unit the avidity of antibodies D. In the General case, in this embodiment, the surface of the biological object in the required areas covered by using separate modules, with, for example, a rectangular shape. Within each segment, radiation sources, for example LEDs, are arranged uniformly. The segment can be made in the form of monolithic integrated chip soldered hybrid led crystal average distance between the surface of the bio-object and LEDs. The individual segments are connected with a flexible coupling spring type and can be located at different distances from each other to form the required geometry of irradiation. In diagram B shows the irradiation of the biological object 1 is already using one large photometric 2 in the form of a semicylinder, on the inner surface of which is placed a large number of LEDs 3. The advantage of this scheme is the possibility of simultaneous exposure as extensive pathology, such as cutaneous manifestations of atopic dermatitis, and at the same time immunocompetent elements of the body, including the thymus, spleen and an extensive network of vessels and nodes of the lymphatic system.

To avoid possible total overload of the organism when exposed, areas that do not require irradiation, can be screened in various ways. The simplest ways is to use curly-cloths, gauze screens or additional aperture.

Other ways are to change the optical axis of each led or a small matrix modules in the desired direction with the help of a computer program to concentrate radiation on the required areas. Another possible method consists in placing between would have been born matix otherwise, for example, due to various electro-optical effects or mechanically due to the relative movement of the diaphragms and valves. And finally, this can be done by changing the level of power, in particular by changing the current through each source, and simultaneously from multiple sources.

6. Photoimmunology device for intraoral use.

For endoscopic applications, the individual radiation sources are not located at the inner surface of the substrate, as described above, and on the outer surface. In biological object, in particular in the intestinal tract, is located a cylindrical capsule with radiation sources. It can be swallowed by a patient and then after passing the stomach naturally recorded in a specific area of the small intestine, which is determined by means of ultrasonic diagnostics.

7. Photoimmunology device for treatment of blood and local treatment of pathologies.

The described invention the matrix on the surface of the hands, can be implemented in the form of flat segments of rectangular shape with dimensions of 15×60 mm For uniform coverage of the hand in the wrist area partition is required, including about the and, one for the local treatment of pathologies. In the case of more extensive pathologies can use up to 4-5 of these sections. Within one segment can accommodate up to 10 LEDs in two rows of 5 LEDs in each row.

8. Photoimmunology device Photoplotter for irradiation of local pathologies.

Form a flexible matrix irradiator close to the plane with a radius of 30 mm wavelength red LEDs 0.63 and infrared of 0.85 μm. The radiation power of about 10 mW at a wavelength of 0.63 µm and 0.3 W at the wavelength of 0.85 μm. The number of LEDs 88. The irradiator on the surface of the body is fixed by the medical adhesive tape or patch. Autonomous power from the battery. Such irradiators may be several simultaneous exposure to multiple zones located in different parts of the body. One light therapy session lasts 15 minutes. All six sessions in one week.

9. Photoimmunology device in the form of a "suit" or "knight's armor".

If necessary, exposure of the whole body you can use the substrate in the form placed on the couch "suit" or "knight armor", the inner surface of which contains many relevant from the authorized geometry with the location of the emitters on their inner surface. These semi-cylinders are placed on a normal couch, on which is located the man.

Such photometrische system is very promising for the treatment of extensive dermatological pathologies, for example in the treatment of infectious diseases in infants, the matrix of LEDs can be attached to the outer transparent to radiation to the walls of conventional incubators. If necessary, exposure of the patient from all sides simultaneously, for example when the total therapy blood, the patient is placed on a transparent for radiation of a couch or a grid type of hammock, which in turn slide inside the cylinder, the inner surface of which emitters repeats the shape of a man.

10. Other photoimmunology device.

In General, as a radiation source in the present invention can be used practically many of the sources used in phototherapy, for example, different types of lasers, incandescent lamps with filters, discharge and fluorescent lamps.

In the case of irradiation of the biological object in a form similar to cylindrical, for example, the limbs of the discharge bulb can be made in the form of thin cylinders, which are evenly spaced around the limbs with axes, parallela irradiator similar design of pump sources in laser systems except only in the region where the active element is placed limb. Depending on the medical task, the sources can operate in different modes spectral ranges mainly from 0.2 to 3 μm with a different degree of monochromaticity of 10-3nm to 103m

The present invention first shown a direct effect of light on the immune system and its modulation by optical radiation. The possibility of selecting the optimal doses of the organism on the basis of avidity antibodies, the degree of immunological failure and individual characteristics of the organism. The interrelation between the exposed area and the number of irradiated zones, wavelength, exposure mode (continuous, pulsed) at different modulation frequencies and duty cycle.

The main advantage of the invention is the possibility of selective and simultaneous exposure of any required extensive areas of the body, directly as the source of symptoms of a particular disease, for example dermatological, and at the same time areas of the Central and peripheral immune system with simultaneous precise quantitative control efficiency rejuv one of the famous inventions that have not previously been reached.

The prospects of combining light with ultrasound, magnetic field and other physical factors of influence, as well as various drugs and immunomodulators. These methods can usefully complement each other and provide significant synergistic immunomodulatory and therapeutic effect.

The present invention can also be used to prevent colds and infectious diseases, especially in the autumn-winter period deficit lighting. There is reason to assume that the seasonal diseases and epidemics of influenza can be associated with a decrease in the activity of the immune system during periods of shortage of light. Therefore it can be recommended for residents of the far North, people whose work takes place in confined spaces, on long space flights, etc., Most cost-effective, low-power, compact and light weight are led matrix.

Brief description of drawings

Fig.1 is an example of dose-response relationships in the form of changes in the avidity of antibodies as a function of time of exposure, where: A - the percentage of vysokoavidnyh tel; t - threshold time at which it starts to change the avidity of the antibodies under action is yuusei therapy, where: 1 - optical effects; 2 - power supply; 3 - block of life support; 4 - biological object; 5 control; 6 - block feedback; 7 - unit sampling and preparation of blood samples; 8 - control unit avidity; 9 - unit corrections impact; 10 - software unit; 11 - a-block with additional sources of exposure (chemicals, ultrasound, etc).

Fig.3 - optical part of the control unit avidity using optical fibers (A) or matrix of light-emitting diodes (B).

A: 1 - radiation source; 2 - optical fiber; 3 - tablet cultures; 4 - cones cultures; 5 - matrix of photodetectors; 6 - multi-unit recording.

B: 1 - a matrix of LEDs; 2 - tablet cultures; 3 - matrix of photodetectors; 4 - multi-unit recording.

Fig.4 - examples fotometricna devices for simultaneous irradiation of various agencies connected with the organs of the immune system.

And sample several photometric: 1 - biological object; 2 - position of the thymus; 3 - position of the spleen; 4 - flat photometric for irradiation of the thymus; 5 - cylindrical matrix for exposure of the extremities; 6 - flexible matrix for the irradiation region of spleen;

B - an example of using one large fot the odes).

Sources of information

1. Illarionov C. E. fundamentals of laser therapy. M: Respect, 1992, S. 26, 31, 71-80.

2. Takushi Tadakuma. Possible application of the laser in immunobiology. The Keio journal of medicine, 1993, 42 (4), 180-182.

3. So Century. Konchugova, S. B. Pershin, A. A. Minenkov. Immunomodulatory effects of low-intensity laser radiation. The journal “problems of balneology”, 1997, No. 1, S. 42-45.

4. T. Karu. Mechanism of low-power laser light action on cellular level. In: Laser in Medicine and Dentistry, Ed. By Z. Simunovic Rijelia: Uitgraf 2000, 98-124, filed Jul. 6, 1998.

5. D. S. Brown, M. R. Brown US Patent No. 6084242 from 06.07.1998, publ. 04.07.2000. Method and Device for stimulating the immune system and generating healing at the cellular level.

6. C. P. Zharov. RF patent №2145247 “Photomedicine therapeutic device for the treatment of extended pathologies” priority from 10.04.1998, published in Bulletin of inventions No. 4, February, 2000.

7. B. C. Ghevondian, N. M.Ghevondyan. Express-method of assessment of functional activity In the immune system by Ghevondyan. RF patent №2137133, priority from, 24.07.1996

8. B. C. Ghevondian, N. M.Ghevondyan, M. C. Ghevondyan. Method and kit for rapid diagnosis of preclinical and clinically significant forms of immunological failure. Application for invention No. 2000105362, priority dated 06.03.2000,

9. B. C. Ghevondian, N. M.Ghevondyan, M. C. Ghevondyan. The way immunocorrective therapy preclinical and clinically the invention No. 2000, priority from 13.03.2000,

10. V. P. Zharov, V. S. Gevondyan, N. M. Gevondyan, S. A. Ermilov, A. M. Volynskya. Light activation of the immune system. Part 1. Influence on G-class antibodies, Proceedings of SPIE. Vol. 3914, 324-333, August, 2000. USA.

Claims

1. The way photoimmunology therapy, including coherent and incoherent radiation natural radiation in the optical range, from x-ray to radio wave of biological objects, including nadkarni, and/or intravenous, and/or in vitro irradiation of blood and/or components and/or external and/or interstitial endoscopic irradiation of the thymus and/or spleen, and/or mucous membranes, and/or lymph nodes, and/or vessels, characterized in that the light matrices perform selective irradiation and/or spatially extended areas of the bio-object associated with the local foci of specific diseases and/or directly and/or indirectly with the Central and/or peripheral organs of the immune system or combined effects on the biological object in the combined exposure with other factors such as physical and/or chemical and/or pharmaceutical preparations and determination of the optimal energy and/or spectral parameters, and/or doses at pre-test IC is s, and/or duration of the treatment cycle is performed on the basis of changes in the ratio of high - and discouvery normal antibodies in serum and/or the secrets of the biological object under test in vitro studies and in the treatment - before, during or after exposure in vivo, while the criterion of achievement immunocorrective effect is the increase in the content vysokoavidnyh antibodies to 75-100% while reducing discouvery antibodies to 0-25%, the criterion of achievement immunosuppressive effect is a decrease in the content vysokoavidnyh antibodies to 5-25% while increasing discouvery antibodies to 75-95%, and the optimal dose effects when treatment is determined on the basis of the transit time of all antibodies from one state of avidity in the other, respectively, when the test exposure in vitro, and also taking into account the severity of immunologic deficiency, the severity of the disease, the irradiated area, and the optimal dose effects on biological object in one session are calculated according to the formula Ewith=To×T×B×S, where Ec- the duration of the session, min; K - correction factor (depends on the device used); T - exposure, min required for the conversion nitrado blood and/or secrets of bioobject in vitro; B - the degree of severity of the disease, assess the scale points: 1 - without clinical manifestations, 2 - mild disease, 3 - average form of the severity of the disease, 4 - severe form of the disease; S - area of exposure.

2. The method according to p. 1, wherein to evaluate the response of the body to influence determination of the level of high and discouvery antibodies in the serum of the patient after the first three sessions.

3. The method according to p. 1, wherein to evaluate the effectiveness of immune-modulating effect and/or duration of treatment is the determination of high and discouvery antibodies in the serum of the patient after the 5th and forth until the 10th session.

4. The method according to p. 1, characterized in that when combined impact on biological object as an additional physical factor can be used optical radiation with different wavelengths, in particular blue and/or red and/or infrared range, and/or x-ray radiation and/or ultrasound and/or temperature, and/or the introduction of oxygen and/or changing the pH, and the effect of various factors carried out sequentially in time with each other and/or simultaneously in edalat based on the maximum change avidity antibodies.

5. The method according to p. 1, characterized in that when combined impact on biological object as additional factors of chemical and/or drug can be used immunomodulators, and/or immunosuppressants, and/or photosensitizers, and/or preparations of immunoglobulins and/or albumin, and/or mixtures thereof, and the optical effects of exercise on biological object to the introduction and/or during introduction and/or after administration of drugs and/or affect themselves preparations, respectively, prior to their introduction and/or after introduction into the biological object, and the time delay between exposure to the drug prior to its introduction into the biological object and/or after administration of the drug and subsequent exposure of the bio-object and/or the optimal dose of exposure is determined based on the maximum change avidity antibodies.

6. The method according to p. 1, characterized in that conduct in vitro irradiation of blood plasma with subsequent reinfuse her into the bloodstream after the conversion of all discouvery antibodies in vysokoavidnyh state.

7. The device immunomodulatory therapy containing optical effects connected with power supply unit, and a control unit, wherein the additional control unit tie is, made with the possibility of information signals in a software unit, and the control unit is configured to implement programmable test modes exposure to serum in vitro before treatment and immune disorders using the parameters defined during the test exposure, the unit of analysis includes a die with cells, the automatic measurement of titles and optical module in the form supplied to the individual cells of the fiber and the radiation receivers and performed as a test of radiation and reception of the effects of absorption and/or scattering in the sediments formed at the bottom of the individual cells.

8. The device under item 8, characterized in that the optical effects of a single and/or combined in a matrix of sources of optical radiation in the form of lasers and/or LEDs, and/or lamps of various types, including incandescent, hid and fluorescent.



 

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