The method of obtaining treniruyuschego antibacterial wound healing products

 

(57) Abstract:

The invention relates to medicine. Spend immobilization of crystalline iodine on native casein goat's milk. Then immobilization of native iodized casein goat milk and mucus glycoproteins of animal origin on the high-molecular compound (Navy) at a temperature of 50 to 55°C. as IUD use methylcellulose. Then add hydrolyzed fucoidan. All components taken in amounts providing the following relations in product, wt.%: glycoproteins of mucus animal 15-20; native casein goat's milk to 34.5-35; methylcellulose 30-35; crystalline iodine 0,5-2,5; hydrolyzed fucoidan, a 9.5 to 17.5. The invention allows to realize the specified destination. 8 C.p. f-crystals, 1 table.

The invention relates to medicine, in particular to the pharmaceutical industry, and is intended to generate treniruyuschego antibacterial wound healing agent.

A method of obtaining a therapeutic tool (patent RU 2172180 C1, 20.08.01), which uses silver nitrate, hydroxide gel, aluminium, polyethylene glycol interferon. Disadvantages are the technical complexity of speciailty.

The purpose of the invention is to develop a new low-cost treniruyuschego antibacterial wound healing products, stable in storage.

For this purpose, the proposed method of obtaining treniruyuschego antibacterial wound healing agent, providing for the immobilization of glycoproteins of mucus and protein at high molecular carrier, followed by the introduction of an antimicrobial agent and a wound healing supplements, protein quality using native iodinated casein goat's milk, which is applied to the methylcellulose at a temperature of 50 to 55°C in a ratio of 1-1, as antimicrobial agent is iodine, as wound healing supplements hydrolyzed fucoidan, as treniruoei supplements glycoproteins of mucus.

The method of obtaining treniruyuschego antibacterial wound healing agent is as follows.

Methylcellulose is brought into a warm water with a temperature of 50-60°C to obtain a 1-1 .5% solution. The mixture was incubated for 1.5-2 hours. Temperature due to the following restrictions. At temperatures below 50°C and above 60°With the process of gelation is longer.

The obtained colloidal gel methylcellulose make is Oseni 1-1 by weight, followed by a gradual cooling for 2-3 hours to a temperature of 10-15°C and the temperature at 10-15°C for 8-10 hours. The separation is based on the method of flocculation.

When making a solution of methylcellulose in a smaller number is not complete precipitation of casein, with the increase of the mass fraction of the solution of methylcellulose output casein remains unchanged, and the flow of components increases. Flocculation at a temperature below 10°C and above 15°C is slower. When making a solution of methylcellulose in not skim milk flocculation goes bad.

This method of concentration of casein is based on two physico-chemical phenomena: limited thermodynamic compatibility of proteins and polysaccharides in water, as well as a higher osmotic pressure of a solution of a polysaccharide in comparison with the osmotic pressure of a solution of protein. Mixing solutions of protein and polysaccharide in certain concentrations leads to the formation of two-phase systems: the lower phase - concentrate casein, top - polysaccharide phase. Through the interface is the transfer of water from the protein solution into the solution of the polysaccharide to establish the phase equilibrium that Obolo">Crystalline iodine in the amount of 0.5 to 2.5% by weight of casein for immobilization is applied by spraying on the received native casein goat's milk with subsequent laundering water free iodine.

Next, prepare 4-5% solution of methylcellulose, putting it in warm water with a temperature of 50-60°C. the Mixture was kept for 1.5-2 hours. Temperature due to the following restrictions. At temperatures below 50°C and above 60°With the process of gelation is longer.

At the same time carry out the selection of glycoproteins intestinal mucus animals (large and small ruminants). This mucous membrane is crushed on the top through the bars of a diameter of 2-3 ml, spend it cleaning and remove glycoproteins by salting out with magnesium sulfate. For canning mucus use alcohol rectified rate of 20% by weight of raw materials.

Native iodinated casein goat's milk, mucus glycoproteins of animal origin and 4-5% solution of methylcellulose contribute in a chemical reactor with a ratio of components of glycoproteins-casein-methylcellulose 1-2-2, where there is a continuous mixing of the components. After 30 minutes in the same reactor contribute hydrolyzed fucoidan in the number of 9.75-17,5% by mass.

The finished product is a dense homogeneous mass brown color with a faint specific odour.

The proposed method of producing treniruyuschego antibacterial wound healing agent is illustrated by examples of its manufacture, are summarized in table.

Treniruyuschie antibacterial wound healing agent can be used in gel form, in the form of cotton-gauze wipes and in freeze-dried form. This tool can also be obtained in the form of films by deposition on a solid basis, followed by drying in air.

Example 1. The mucus glycoproteins of animal origin and iodinated casein goat's milk mixed with 4-5% solution of methyl cellulose in a ratio of components glycoproteins of mucus-iodinated casein-methylcellulose as 1-2-2 at a temperature of 50 to 55°C. the resulting mixture was stirred for 15-20 minutes. Next, make a hydrolyzed fucoidan in the number 17,50% by mass.

Used in gel form, which advantages over other forms used for the same purpose, is that it is well applied to the skin, the wound surface, well-distributed and easily smiffys the specified components included in the product in the following ratio, wt.%:

Native goat casein 34,50

Glycoproteins of mucus animal 17,75

The Methylcellulose 32,00

Crystalline iodine 1,75

Hydrolyzed fucoidan 14,00

Next, the resulting gel impregnated multilayer gauze napkins of various sizes (from 5×5 cm to 30×30 cm). Tissues have high stability and duration of action, atraumatic. Using napkins allows to reduce the duration of treatment. Reduced consumption of drugs and dressings for treatment, facilitated the work of the medical staff. It is particularly convenient to use napkins in the military field, in the aftermath of natural or man-made disasters and mass lesions of the people.

Example 3. Carried out as in example 1, except that the specified components included in the product in the following ratio, wt.%:

Native goat casein 35,00

Glycoproteins of mucus animal 20,00

The Methylcellulose 35,00

Crystalline iodine 0,50

Hydrolyzed fucoidan 9,50

Next, the resulting gel is dried using sublimirovanny and milled. The powder is applicatio the displacement of the affected area, reducing the toxic load on the system of detoxification. This form demonstrates to the local application to persons of any age for the prevention and treatment of inflammatory processes of the skin, rash, acne, diaper rash, weeping eczema, various dermatoses, trophic ulcers, complicated by secondary infection. Used topically as a powder.

This tool can also be obtained in the form of films, by drawing on a firm basis, followed by drying in air.

Insertion of an IUD makes the tool plasticity and the formation of a film when applied to the skin increases with prolonging the action of the tool. Introduction casein provides effective drainage in the weeping wounds and the formation of the porous structure obtained funds facilitates the access of oxygen to the wound and speeds up healing. The mucus glycoproteins of animal origin provides effective drainage and encapsulation products intoxication wounds. Hydrolyzed fucoidan speeds up the process of epithelialization of the wound, and the iodine acts as an antibacterial agent and provides stabilization funds from microbiological contamination.

Put a thin tool means on volunteers aged 18-30 years show, that the drug is effective, well tolerated, safe, does not cause allergic reactions.

1. The method of obtaining treniruyuschego antibacterial wound healing products, including stabilization and drying of the drug, wherein spend immobilization of crystalline iodine on native casein goat's milk, then immobilization of native iodized casein goat milk and mucus glycoproteins of animal origin on the high-molecular compound (Navy) at a temperature of 50 to 55°C, which is used as methylcellulose, followed by the addition of hydrolyzed fucoidan, and all the components taken in amounts providing the following relations in product, wt.%:

Glycoproteins of mucus animal 15-20

Native casein goat's milk to 34.5-35

The Methylcellulose 30-35

Crystalline iodine 0,5-2,5

Hydrolyzed fucoidan, a 9.5 to 17.5

2. The method according to p. 1, in which the Navy used 4-5% solution of methylcellulose brand MC-100.

3. The method according to p. 1, characterized in that use casein allocated from skimmed goat's milk flocculation, using as a flocculant 1-1 .5 m is the Method according to p. 1, characterized in that the flocculation is performed by mixing the components after heating milk to a temperature of 40÷45°With, followed after mixing gradual cooling over 2 hours to a temperature of 10÷12°C.

5. The method according to p. 1, characterized in that used as a wound healing agent hydrolyzed fucoidan administered in the amount of 9.5 to 17.5 percent by weight.

6. The method according to p. 1, wherein the mucous membrane is crushed on the top through the bars of a diameter of 2 mm, spend it cleaning and remove glycoproteins by salting out magnesium sulfate.

7. The method according to p. 1, characterized in that treniruyuschie antibacterial wound healing agent can alcohol and packaged in sterile containers, pre-treated with hydrogen peroxide.

8. The method according to p. 1, characterized in that treniruyuschie antibacterial wound healing agent can be obtained in lyophilized form, gel form or in the form of cotton-gauze napkins.

9. The method according to p. 1, characterized in that treniruyuschie antibacterial wound healing agent can be obtained in the form of films, by drawing on a firm basis with subsequent Sushko is

 

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