A method of creating a grassy lawns and care for him

 

(57) Abstract:

The invention relates to the field of construction, in particular the field of creation of soddy coatings and care for them, and can be used for the establishment and operation of outdoor sports soddy sites, high-quality lawn areas (tennis courts, Golf courses), ornamental lawns and soil-protective coatings slopes. When creating the turf into the soil before sowing seeds contribute additional porous structure formers soil on the basis of natural aluminosilicates. The first Supplement contribute simultaneously with the first watering or adsorbed on the porous structure formers. Second Supplement make subsequent irrigations. The first Supplement contains non-pathogenic live microorganisms that contribute to the development and strengthening of the root system. The number entered with the first Supplement of the cells is not less than 106CL/m2the lawn surface. The second Supplement contains yeast autolysate in the amount of 1-10 g per 1 DM3watering solution. The first watering is carried out in an amount of 2.0 to 5.0 DM3solution/m2the lawn surface, and subsequent watering is carried out in an amount of 0.3 to 3.0 DM3watering solution. Usually as a porous structure formers use perlite, ceramsite and ility in the form of granules or particles with a size of 0.2-2 cm and a bulk weight of 0.3-0.7 kg/DM3. Mainly the first Supplement contains an Association of living cells of microorganisms Bejierickia fluminensis, Thielavia terrestris ratio of crops in Association 1:10 -10:1. The second Supplement is an autolysate of cell culture Pichia carsonii, containing a complex of vitamins, proteins and peptides. It provides improved quality of grass cover, the strengthening of the turf due to the development of more robust and extensive root system. 10 C.p. f-crystals.

The invention relates to the field of construction, in particular the field of creation of soddy coatings and care for them, and can be used for the establishment and operation of outdoor sports soddy sites, high-quality lawn areas (tennis courts, Golf courses), ornamental lawns and soil-protective coatings slopes.

Known (US patents 4435202 and 4104052) plant growth regulators representing chemical compounds of different composition, affecting the rate of development of plants, but there is no data about their selective impacts the ptx2">Known (US patent 6219965) method of cultivation and care of the sod coating, including the creation of multi-layered gazopodobnogo coating, laying palaikastro lamellar structure as the sward. However, this solution does not make recommendations about the process of growing grass and not given information about how to use it in relation to the lawns for various purposes.

Known (US patent 3960540) a method comprising the use of chemical compounds to regulate (inhibit) the growth and height of grass in dernova layer, but this solution does not contain recommendations on software developed root system of the grass, the creation of high-quality lawns and turf.

Known (US patent 6357175) a method for increasing the growth and survival of the herbs in cold and warm seasons, providing for the ozonization of air and contact ionized gas mixture with dispersed ejector on submicron droplets of water that provides a high level of dissolved oxygen. However, in the patent there is no data about the effectiveness of development actually the root system of the grass for both the persons caring for grass outdoor sports facilities. According to this method, the prepared soil sites seeded, and then periodically watered and mowed grown grass. The disadvantage of this method should recognize the lack of quality of the lawn, due to a weak turf.

Known (RU, patent 2100515, E 01 13/00, 1997) is a method of obtaining cultural lawn and care for him. According to a known method, pre-make mineral and organic fertilizers, plant the seeds in the prepared soil, seeds, periodically watered and weed obtained lawn and peresashivayut the damaged areas of the lawn and so on

The technical problem solved by the present invention is to develop a method of creating a grassy lawn covering open areas and care, and improved quality of grass strengthening of turf at the expense of achieving a more robust and extensive root system of plants.

The technical result is obtained in the result of implementation of the invention consists in strengthening darmowego coverage by obtaining enhanced root structure and producing quality turf surface.

To achieve the specified technical fertilizer into the soil, sowing seeds, watering sprinkler and regular mowing of the grass and into the soil before sowing seeds contribute additional porous structure formers soil on the basis of natural aluminosilicates, as well as the first Supplement that contribute simultaneously with the first watering or adsorbed on the porous structure formers, and a second Supplement make subsequent irrigations, the first Supplement contains non-pathogenic live microorganisms that contribute to the development and strengthening of the root system, and the amount introduced with the first Supplement of the cells is not less than 106CL/m2the lawn surface, and the second Supplement contains yeast autolysate in the amount of 1-10 g per 1 DM3watering solution, with the first watering is carried out in an amount of 2.0 to 5.0 DM3solution/m2the lawn surface, and subsequent watering is carried out in an amount of 0.3 to 3.0 DM3solution/m2the lawn surface using the second supplements when its content for the occasional introduction of 1-10 g per 1 DM3watering solution. Usually as a porous structure formers use perlite, ceramsite and ility in the form of granules or particles with a size of 0.2-2 cm and a bulk weight of 0.3 fluminensis, Thielavia terrestris ratio of crops in Association 1:10-10:1. The second Supplement is an autolysate of cell culture Pichia carsonii, containing a complex of vitamins, proteins and peptides, and has the concentration of amino nitrogen for at least 30 mg/ml is Usually the autolysis of cells Pichia carsonii spend when their concentration in the environment of 1-2 wt.%, at a temperature of 45-55C for 12-24 hours Preferably the first Supplement is used for irrigation in the form of an aqueous suspension of microorganisms at concentrations of 106-109cells/ml. In some embodiments of the method of the first supplements that contain live microorganisms adsorb on the builders before depositing them into the soil, 1 g amendment contains 104-108cells. Usually the adsorption of cells of microorganisms in porous natural aluminosilicates carried out by stirring them with cell suspension within 2-4 h at a ratio of solid and liquid phases of 0.5:1-2:1, or in the process of growing microorganisms, the ratio of solid and liquid phases is 0.05:1-0,5:1. When applying the first supplements (or by irrigation or adsorbed) by 1 m2the lawn surface, it is preferable to make 106-1012live the AI method is used strains of microorganisms, deposited in Russian national Collection of Industrial Microorganisms (VKPM) under the following numbers: Bejierickia fluminensis B-8276, Thielavia terrestris F-868, Pichia carsonii Y-3022.

Incorporated into the soil in a given number of porous strukturoobrazovatelja allows you to create the most favorable conditions for the development of the root system of turf grass due to adsorbed thereon directly or during irrigation dietary supplements. Use as a builders exactly minerals from the group of silicates is due to a relatively high absorptive capacity that allows it to retain on their surface used solutions of dietary supplements in the form of a cell suspension or autolysate, as well as their ability under the influence of moisture gradually transformed into clay kidney. Used in the first Supplement, the Association of microorganisms, particularly those containing these strains, capable of stimulating the development of the root system of turf grass. The contents of the cells of the microorganisms in suspension selected empirically and brings the maximum effect. While selected experimentally Association of these crops in the Supplement provides the best supply of the root system rosaniline roots and creating a solid turf surface. Additional significant effect in creating and maintaining turf, as well as strengthening the sward provides for the application of subsequent after planting seeds, watering the second supplements containing autolysate yeast Pichia carsonii. Active development of microorganisms of the first supplements promotes complex vitamins, particularly the b group, amino acids and peptides in the second Supplement, obtained by the process of autolysis of the cells. Thus, it was found experimentally synergistic effect of two dietary supplements and their method of use allow you to get the greatest effect. Given in the dependent claims, the quantitative ratio also installed experimentally on the basis of estimates obtained a positive result.

In the future, the proposed method will be discussed using examples of implementation.

1. The experiments were conducted under laboratory conditions in a growth chamber with a photoperiod of 15 hours, humidity 60%, at a temperature of 25°With the lighting and 14°C in the dark. The light source was a greenhouse lamp. In sod-podzolic soil made of mineral and organic fertilizers to the t in the form of granules with a size of 0.2-1.0 cm density 0.5 kg/DM3and brought the seeds of grasses mixture at the rate of 0.5 g / 100 cm2square planting. Prepared for irrigation water solution supplements, adding irrigation water first Supplement is in the form of a suspension of living organisms Thielavia terrestris and Bejierickia fluminensis, taken in the ratio 1:1. The percentage of total cells in an aqueous solution of the first supplements was 106cells/ml Produced watering at 2 DM3/m2while the measured cell concentration in the soil was calculated for 1 m2108cells.

Subsequent irrigations were carried out every three days in the amount of DM3/m2while in irrigation water were added to the second Supplement is in the form of autolysate yeast Pichia carsonii in an amount of 10 g/DM3. Mild autolysis of yeast was carried out at T=50°C for 20 h, the content of amino nitrogen in the autolysate was 36 mg/ml

After 10 days of cultivation of all plants were removed from soil, washed with tap water, dried, weighed, cut off the root part and weighed again.

The total increase of green mass compared to control was 35%, including an increase in the mass of roots was 65%, while the root system was more R the field conditions at 1 m2the lawn surface. As porous amendment used perlite with a particle size of 1.5-2 cm and a bulk weight of 0.7 kg/DM3that was made in the soil before sowing the grass seeds in the control plot. On the experimental plot and the control plot soil was added organic fertilizer in the form of superphosphate, potash fertilizers, compost. The first Supplement was an Association of cultures Thielavia terrestris and Bejierickia fluminensis, taken in the ratio 1:4, at a total cell concentration of 108cells/ml Simultaneously with sowing seeds of spent irrigation water solution when the solution cell 109cells/ml at a rate of 5 DM3/m2. While in the taken samples were 10 cells Thielavia terrestris and Bejierickia fluminensis per 1 m2the lawn surface.

Subsequent irrigations were performed after 4 days in the amount of 0.5 DM3/m2by watering water the second Supplement on the basis of autolysate yeast Pichia carsonii in an amount of 10 g/DM3solution. Supplements were obtained by autolysis of yeast at 50°C for 12 h, the content of amino nitrogen was 30 mg/ml After 12 days was determined that the total increase of green mass was on the experimental part is inoe their branching, that provided a strong sod layer on the control plot.

3. Creating lawn was carried out similarly to example 2, but in the Association of the ratio of the cultures of the Thielavia terrestris and Bejierickia fluminensis was 6:1. As the amendment was applied natural aluminosilicate the Irlit with an average particle size of 1-1,5 cm and a bulk weight of 0.6 kg/DM3. The total increase of green mass in the experimental area compared with the control was 20%, and the increase in mass of roots including 52%. Lawns on the experimental plot was thicker and more durable turf.

4. Creating turf was carried out similarly to example 3, but applied the amendment expanded clay granules with an average size of 1.0-1.2 cm and a bulk weight of 0.6 kg/DM3pre, before the soil was saturated by the first Supplement. The adsorption of the clay was performed in a volume of 10 l with stirring at 200 rpm for 3 h, when the ratio of solid and liquid phase (suspension of microorganisms) of 0.5:1. The first formulation contained the Association grown microorganisms Thielavia terrestris and Bejierickia fluminensis, taken in the ratio 1:1. After adsorption of 1 g of the clay contained 104cells of microorganisms. After planting seeds in the soil RA>the cultivated area amounted to 107cells. Subsequent irrigations were carried out as in example 3. The total increase of green mass was 31%, including root mass increased in weight by 62%, sod layer was solid.

5. Creating turf was carried out similarly to example 4, but the clay was saturated culture of microorganisms during growth of microorganisms Thielavia terrestris and Bejierickia fluminensis. In a fermentation medium for cultivation of microorganisms contributed clay, the ratio of the solid and liquid phases was 0.05:1. The cultivation was carried out for 2 days at a temperature of 32°C, stirring at 200 rpm on a nutrient medium containing sources of nitrogen, phosphorus, magnesium, manganese, iron. After completion of the adsorption process in 1 g of clay contained 105cells of the microorganisms, the ratio of cells of microorganisms Thielavia terrestris and Bejierickia fluminensis was 1:5. In the analyzed soil sample was determined by the content made of living cells, which amounted to 2.108cells per 1 m2the lawn surface.

The experiment was obtained an overall increase of green mass of 37%, including the increase in root weight was 69%. The turf on the control plot would be ebonyromance in the all-Russian Collection of industrial microorganisms (VKPM) under the numbers: Thielavia terrestris F-868, Bejierickia fluminensis B-8276, Pichia carsonii Y-3022.

These examples illustrate but do not limit possible implementations of the proposed method.

1. A method of creating a grassy lawn coverage and care, including fertilization in the soil, sowing seeds, watering sprinkler and regular mowing of grass, characterized in that the soil before sowing seeds contribute additional porous structure formers soil on the basis of natural aluminosilicates, as well as the first Supplement that contribute simultaneously with the first watering or adsorbed on the porous structure formers, and the second Supplement, which make subsequent irrigations, the first Supplement contains non-pathogenic live microorganisms that contribute to the development and strengthening of the root system, with the number entered with the first Supplement of the cells is not less than 106CL/m2the lawn surface, and the second Supplement is a yeast autolysate in the amount of 1-10 g per 1 DM3watering solution, with the first watering with the use of the first supplements is carried out in an amount of 2.0 to 5.0 DM3solution/m2the lawn surface, and subsequent irrigations using second is about on p. 1, characterized in that the porous structure-formers use perlite, ceramsite and ility in the form of granules or particles with a size of 0.2-2 cm and a bulk weight of 0.3-0.7 kg/DM3.

3. The method according to p. 1, characterized in that the first Supplement contains an Association of living cells of microorganisms Bejierickia fluminensis, Thielavia terrestris ratio of crops in Association 1:10 -10:1.

4. The method according to p. 1, characterized in that the second Supplement is an autolysate of cell culture Pichia carsonii, containing a complex of vitamins, proteins and peptides, and has the concentration of amino nitrogen for at least 30 mg/ml

5. The method according to p. 4, characterized in that the autolysis of cells Pichia carsonii spend when their concentration in the environment of 1-2 wt.% at a temperature of 45-55 within 12-24 hours

6. The method according to p. 1, characterized in that the first Supplement is used for irrigation in the form of an aqueous suspension of microorganisms at concentrations of 106-109cells/ml.

7. The method according to p. 1, characterized in that the first supplements that contain live microorganisms, additionally adsorb on the builders before depositing them into the soil, 1 g amendment contains 104-108cells.

8. Str is changed by mixing them with cell suspension within 2-4 h at a ratio of solid and liquid phases of 0.5:1-2:1.

9. The method according to p. 7, characterized in that the adsorption of cells on porous aluminosilicates carried out in the process of growing microorganisms, the ratio of solid and liquid phases is 0,05:1-0,5:1.

10. The method according to p. 1, characterized in that the number of living cells of the first supplements that are made at 1 m2the lawn surface is 106-1012cells.

11. The method according to p. 1, characterized in that the supplements obtained using strains of microorganisms Bejierickia fluminensis In-8276, Thielavia terrestris F-868, ichia carsonii Y-3022.



 

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