Way rehabilitation sperm of boars manufacturers


(57) Abstract:

The invention relates to veterinary medicine. The method comprises bubbling glucosegalactose-sulfate environment ozone-oxygen mixture with ozone concentration of 5 mg/l within 30 minutes Method causes symptoms in the environment antimicrobial properties. The use of this medium for dilution of sperm manufacturers improve the sanitary quality of sperm and increases fertility after insemination of sows. 1 PL.

The invention relates to veterinary medicine, in particular to the technology of artificial insemination, and can be used for the renovation of the sperm of boars manufacturers.

Known manual method of obtaining semen from boars. Sperm obtained by this method are of high quality and has a low bacterial infection [1].

The disadvantages of the manual method, you should include the fact that it requires from staff great physical stress and overcome the psychological barrier. Increases time spent on obtaining sperm, because the operator is located near each of the boar charge from the beginning to the end of ejaculation.

Known methods of rehabilitation of sperm about what abiotical and sulfonamides, which have bacteriostatic activity against most gram-negative and gram-positive microorganisms [2, 3].

The disadvantages of sanitation preparations should include the fact that they are not effective due to the emergence of resistant strains of pathogenic and conditionally pathogenic microorganisms, creating them impossible to use with low sperm quality and have a high cost.

The proposed method of remediation sperm includes common with the prototype of the sign is the presence in synthetic environments antimicrobial substances. In relation to the prototype characteristic of which is pre-bubbling glucophageglucophage-citrate (GHCS) environment that is used to dilute semen of boars, ozone-oxygen mixture at a concentration of 5 mg/l within 30 minutes.

Preparation of ozonated GHCS environment was passing through it of ozone-oxygen mixture at a concentration of 5 mg/l within 30 minutes. Pre GHCS environment in quantities of 1 liter was poured into plastic bottles of 1.5 litres, closed by a stopper with two holes. through which passed two hoses made of polyurethane. One hose was attached ceramic races who begins exhaust gas.

Microbiological studies revealed that ozonated GHCS environment has a strong bactericidal effect against Staphylococcus aureus, Proteus vulgar, Pseudomonas aeruginosa and Escherichia coli.

When determining the optimal concentration and time of ozonation ozone-oxygen mixture GHCS environment was based on the antimicrobial effect of ozone, its effect on gametes and reproductive quality of sows. The studies found that the optimal concentration is 5 mg/l within 30 minutes.

Research and production experience held on the main sows of large white breed and cross-breed weighing 180-220 kg Animals in the experimental group (n=190) were seminal sperm, diluted ozonized GHCS environment. Dilution was performed fractional: initially half degree and again 10 minutes before insemination to the recommended dilution. Sows of the control group (n=200) were seminal sperm, these same manufacturers, but when diluted ejaculates GHCS environment with poligram. Artificial insemination of sows spent ungraded way, after the establishment of the immobility reflex with a boar is probe, twice, perlocutionary the ability of sperm, the duration of pregnancy, the incidence of sows postpartum endometritis, multiple pregnancy, weight piglets and safety are presented in the table.

As can be seen from the table, fertility, duration of gestation sows was in the experimental and control groups are almost identical, which confirms the evidence that the ozone does not violate the processes of fertilization and pregnancy. When observing animals in the postpartum period, found that the incidence of sows acute postpartum endometritis in the group where sanitation sperm conducted saturated with ozone synthetic environment, which was 4% less than when using antibiotikoterapia drug poligen. The prolificacy of sows had no significant differences, and the number of stillborn piglets was lower in the experimental group was 55.6% compared with control. The live weight of piglets at birth in the group that used ozone-oxygen mixture was higher by 7.7% (P<0,05). The effect of krupnoplodniy affected the viability of the animals in the experimental group the safety of piglets on day 21 was higher by 8%, and the live weight of piglets at 3%.

Direct material costs for the rehabilitation of scooterzone drugs.

Thus, the proposed method of remediation sperm of boars manufacturers ensures the maintenance of high fertility of sows, allows to obtain a large and viable offspring with the lowest material costs.

Sources of information

1. Sheiko E., Zubov So, Linkevich E., Mikhailov, I., Podskrebko N. The sperm quality of boars in dependence from the methods it takes //the Pig, - 1999. No. 2. - S. 30-32.

2. Manual on artificial insemination of pigs. M.: Kolos, 1976, - 18 C.

3. Soetkin S. Century, The Birthplace Of C. N., Smirnova Century So Narizny A., Sanitation sperm of boars by poligram and Gump // Zootechnics. - 2000. No. 4. - N-30-31 - a prototype.

Way rehabilitation sperm of boars manufacturers, including the use of glycosolation-sulfate medium, characterized in that glucosegalactose-sulfate environment bubbled ozone-oxygen mixture with ozone concentration of 5 mg/l within 30 minutes


Same patents:

The invention relates to the fishing industry

The invention relates to veterinary

The invention relates to the field of veterinary medicine

The invention relates to agriculture
The invention relates to animal husbandry, in particular to a method of breeding pigs with the help of artificial insemination

The invention relates to livestock and can be used in artificial insemination of sheep

The invention relates to animal husbandry

The invention relates to animal husbandry

The invention relates to animal husbandry

The invention relates to animal husbandry, in particular to methods of diagnosis of multiple pregnancy pregnant sows

FIELD: medicine.

SUBSTANCE: method involves using product containing spermatozoa treated by drying with freezing to humidity level of 1% and having injured membrane or spermatozoon head so that spermatozoon nucleus retains its genetic validity enough for fertilization. The spermatozoon heads fertilize an isolated oocyte after rehydration and microinjection being done. The retained genetic integrity is enough for fertilizing an oocyte and producing living descendants. Method involves collecting living mature spermatozoa, making spermatozoa suspension in special purpose physiological medium, freezing the spermatozoa suspension for producing frozen spermatozoa, drying the frozen spermatozoa or spermatozoa heads in vacuum to humidity level of 1%, making rehydration of spermatozoa or spermatozoa heads with injured membrane and selecting those retaining nucleus of genetic integrity. The selected spermatozoa or spermatozoa heads are used for fertilizing isolated oocytes with living descendants being produced.

EFFECT: enhanced effectiveness in producing living descendants.

39 cl, 5 dwg, 1 tbl

FIELD: veterinary science.

SUBSTANCE: the suggested preparation contains condensate obtained after distillation with urinary vapor of sows being in heat, acetic acid, butyric acid, caproic acid at the following ratio of components, weight%: acetic acid 0.5-2.0, butyric acid 5.0-20.0, caproic acid 0.5-5.0, condensate - the rest up to 100. The preparation is of high biological activity.

EFFECT: higher efficiency.

1 ex, 1 tbl

FIELD: animals science.

SUBSTANCE: the present innovation deals with intramuscular injection of sodium salt preparation cloprostenol 30-45 min before placing at the dosage of 750 mcg/animal. The method provides increased reproductive function, enhances sexual reflex, increases the volume of ejaculate, concentration, activity and quality of spermatozoa.

EFFECT: higher efficiency of breeding.

2 ex, 3 tbl

FIELD: animal science.

SUBSTANCE: it is necessary to obtain bovine sperm, followed by evaluation, dilution and packing into polymeric microcontainers due to vacuum to be sealed from both ends. One end should be sealed with hermetic substance consisting of an alloy to cover solid rennet cheese 60% and paraffin 40% against total volume. Then, under conditions of isolated chamber, at excessive pressure of sterile air individual diluted bovine sperm should be packed with the help of vacuum. The second end of microcontainer should be sealed due to impregnating in antibiotic-treated hen yolk for 1-2 sec to be then equilibrated.

EFFECT: higher quality of sperm.

FIELD: veterinary science.

SUBSTANCE: the suggested composition contains based upon 100 ml distilled water: from 0.25 to 4.5 g sodium citrate, from 0.45 to 4.0 g dextrose, from 0.01 to 0.3 g penicillin, from 50 000 to 500 000IU streptomycin, from 0.25 to 0.45 g catalase, from 0.045 to 0.25 g yolk, from 1.5 to 15 ml glycerol and from 0.02 to 0.1 mcg canine blood serum. The innovation keeps canine sperm for prolonged period of time, maintains viability and mobility of spermatozoa and provides high spermatic fertility after defrosting.

EFFECT: higher efficiency.

2 ex

FIELD: animal science.

SUBSTANCE: the present innovation deals with sanitary-hygienic preparation of exercising area, equipment and animals before sampling the sperm in breeding animals. One should clean the floor in exercising area with catholyte at pH being not less than 11.0 and redox potential - not less than -600 mV; air and floor in exercising area, special equipment for animals as service ramps, artificial vaginas and sperm-collecting reservoirs should be disinfected with anolyte at pH being not more than 2.5, redox potential - not less than 1100 mV and active chlorine content of not less than 0.03%; one should wash animals skin, scrotum and prepuce with anolyte at pH being 7.0±0.5 and redox potential of not less than 900 mV. Moreover, one should treat the air in exercising area with anolytic aerosol at liquid drops diameter being not more than 50 mcm at exposure of not less than 30 min, as for artificial vaginas and sperm-collecting reservoirs they should be kept in anolyte for 30-60 min. The innovation provides to obtain high-quality sperm in farm animals.

EFFECT: higher quality of disinfection.

1 ex

FIELD: veterinary science, virology.

SUBSTANCE: method for evaluation of semen from bull-sires for contamination with cattle infectious rhinotracheitis virus involves taking semen samples, combining semen samples obtained from bull-sire for one month and their analysis as a single sample. The analysis is carried out by method of molecular hybridization. For evaluation of semen from rejected bull-sires the semen samples are taken obtained for all period of their exploitation. Method provides reducing labor intensity and time for analysis and enhanced sensitivity of analysis in assay of semen contamination with indicated virus.

EFFECT: improved method for evaluation of semen.

2 cl, 1 tbl, 4 ex

FIELD: zooculture.

SUBSTANCE: thermostat for transporting agricultural animals' semen has case, cap and lower working part. Working part consists of two mutually perpendicular hemispheres disposed one inside the other. There are containers with free laying freights at the lower part of hemispheres. Thermostat also has thermoregulator, container with sperm and insulating material, which keeps integrity of the container.

EFFECT: periodical stirring of sperm; prevention from strong shakes.

2 dwg

FIELD: animal science.

SUBSTANCE: the medium for sperm cryoconservation should be supplemented with FLPG preparation - the complex of synthetic analogs of prostaglandins F, E, E1, E2 deposited in vegetable oil at concentration being 125 mcg/ml diluted sperm. The innovation enables to increase fertilizing capacity of ram's sperm, stimulates reproductive function in ewes due to positive impact onto ovicells and spermium movement to the site of fertilization.

EFFECT: higher efficiency.

2 ex, 3 tbl

FIELD: medicine, artificial insemination.

SUBSTANCE: the innovation consists of the following stages: selection of spermatic cells from a male of a certain mammalian type except a human being; introduction of spermatic cells into the flow of fluid medium; analysis of spermatic cells taken by the flow of fluid medium; detection of DNA quantity in the nuclei of spermatic cells; irradiation of dyed DNA in the nuclei of spermatic cells; detecting the fluorescent light transmitted by irradiated dyed DNA being in the nuclei of spermatic cells; differentiation of spermatic cells based upon the quantity of DNA being inside the nuclei of spermatic cells located in the present drop; electrostatic deviation of every drop; separate accumulation of drops based upon the quantity of DNA inside the nuclei of spermatic cells captured into each drop; separation of differentiated spermatic cells into X-chromosome-carrying populations and Y-chromosome-carrying ones; and obtaining the populations of intact X-chromosome-carrying and Y-chromosome-carrying spermatic cells at purity degree being above 90% for each. The innovation enables to improve separation of spermatozoa and increase its velocity.

EFFECT: higher efficiency.

19 cl, 43 dwg