Method to determine stress resistance cows
The invention relates to the field of veterinary medicine. Stress cows is determined with the aid of the device of electro PERT-4M by measuring the electrical conductivity of biologically active points of the skin Ten-man responsible for the condition of the Central nervous system. The readings on the positive and negative potentials (symmetry or asymmetry in the variance of the arrow on the gauge) is the conclusion about the stability or sensitivity of the nervous system of an animal to stress factors. Stress is diagnosed when the difference value is less than 2.5 μa, unstable stress at 2.5 to 5 μa, stressoustiychivost when the difference values greater than 5 μa. The method is easy to implement, allows to determine the stress cows, irrespective of the value of milk yield, the period after calving and stage of the sexual cycle.
The invention relates to the field of veterinary diagnostics, in particular the testing of cows on stress and stressoustiychivost.
At present readily available and reasonably informative methods for the simultaneous determination of the functional abilities of both specific and non the species.
There is a method of determining the stress resistance of cows on the content of cortisol in the blood.
Based on the study of the dynamics of cortisol in the blood after stressaway cows produce 3 types of stress resistance is high, unstable, and low.
For animals with high stress inherent in rapid normalization of homeostasis; after the manifestations of the stress response in 30 minutes cortisol levels returned to baseline values.
The type of animals with low stress is characterized by the fact that after a short-term normalization of the content of stress hormones in the blood is prolonged lowering of their level, which can last up to several days .
Determination of hormones involved in only the special laboratories or departments of larger clinical laboratories, and this requires certain conditions.
There is a method of determining the stress resistance of cows at a load of adrenocorticotropic hormone (sample Torna).
Pre-blood count eosinophils. Then intramuscularly adrenocorticotropic hormone at a dose of 25 units per 100 kg of body weight. Count the eosinophils in the blood after 2 and 4 hours. In the military sensitivity of the animal to stress .
There is a method of determining the stress resistance of cows at a load of adrenaline.
Pre determine blood glucose. Then adrenaline intramuscularly at a dose of 1 ml of 0.1% solution per 100 kg of body weight. Determine the content of glucose in the blood every 30 minutes for 3 hours. Hyperglycemia in the first or second capture more than 50% and not returning to its original state after 3 hours, speaks about high stressoustojchivosti cows .
These two tests have an indirect value, and they are best used in combination with other tests.
There is a method of determining the stress resistance of cows with emotional stress.
In cows evaluation of resistance to stress can be run on lactational function. Stress is milking cows milkmaid not previously douchey this cow. The stress of cows milk yield decreases not more than 25%, the same is lengthened and the time of milking. These indicators are returned to their original condition within three days. Stressoustojchivye cows reduce productivity and increase the milking time by 50% or more. Return to the original state is observed after 4 days or more .
ptx2">For assessment criterion type of stress resistance cows when this method is used, the intensity of the inhibition of lactation. Indicator reflex lactation are the most informative indicators of milk production and milking time. It is recommended to determine such factors as videonote for the first minute of milking, the responsiveness at the beginning of milking, the volume change of the nipple. Therefore, this method is time consuming, involves labor of mespecialist. Preferably, this method was combined with the assessment of the suitability of cows to machine milking, at which, according to the instructions on the evaluation, determine milk yield and milking time share udder.
In addition, in this method the assessment of the cows can be made in the period 1-7 th months after calving, when the milking least 3-4 kg; and in the days of oestrus assessment conduct is undesirable due to the increased excitability of the cows.
The purpose of the proposed method is to develop a simple, affordable way to test cows for stress resistance and stressoustiychivost.
This goal is achieved by the fact that with the help of the device erpt-4M by electric shocks on biologically active point (BAP) skin Ten-Maine, located on Sredinnyi animal to stress factors.
The device PERT-4M (the unit of electro - microammeter) issued Dnipropetrovsk machine-building plant, approved BOO WITH 28900. It is designed for finding contact method BAHT skin, diagnostics of the functional state of internal organs conductivity corresponding to the organ BATH and therapeutic effects on these points weak constant rectangular-pulse electric current of changing polarity.
In medicine to determine the exact localization of the BATH is carried out according to the method, taking into account the confirmation of the location of certain sensations (feeling cold, heat, pain, and so on) whose information comes from the patient. In veterinary practice, this method is not applicable, therefore the device erpt-4M is provided by the finding of the BAT on the principle of determining the least electrical resistance of the skin in the area of the point relative to the adjacent areas of the skin of the animal.
The selection of BAT skin corresponding to the Central nervous system, is carried out according to the data presented by M. C. Platinum . This point Teng-man.
In the electro-puncture for diagnostic purposes using two main parameters - the value of biopotential BAHT skin and Atika conducting electro-study the following: check the charge level of the device power supply and set the initial value of the output current is 20 µa. Then indifferently negative electrode clamp is fixed on podgotovki the crease of the animal. The choice of the place of fixation due to the fact that the fold does not have hair, moist, which ensures a reliable contact with the patient's body and is the reference area.
Area localization BATH is treated with alcohol. The search points are active positive electrode to the positive polarity by measuring the lowest electric resistance (visually on the maximum variance direction microammeter). After finding BAHT electrode is fixed at a point using a special suction Cup, which ensures uniform pressure of the electrode on the skin in the study of points in all animals of the experimental group. The magnitude of the supplied current increases smoothly until the first signs of anxiety of the animal, and then decreases by 5-10 μa, and fixed the value of the electrical conductivity of the skin corresponding to the threshold of pain sensitivity. Then the device switches to a negative polarity, and after the firm establishment of the arrow microammeter is fixed result. The value of asymmetry testimony reflects nerve conduction in reflex S="ptx2">Stress cows is determined with the help of the device erpt-4M method of measuring conductivity BAHT skin Teng-man.
The readings on the positive and negative potentials (symmetry or asymmetry in the variance of the arrow of the device) it is concluded that the susceptibility or resistance of the nervous system of an animal to stress factors.
If the conductivity of the occipital BAHT difference between positive and negative potentials is less than 2.5 μa, cow assessed as resistant to stress. When the potential difference point Ten-man 2.5-5 µa cow belongs to the group of animals with unstable stress. The cow with the asymmetry between positive and negative polarity of the device more than 5 μa, consider stressoustoicivij.
Comparative analysis shows that the inventive method differs from the prototype in that it is easy to implement, requires minimal time veterinary specialist, facilitates his work. This way you can determine the stress cows, irrespective of the value of milk yield, the period after calving and stage of the sexual cycle.
Comparison of the proposed method with others is both not only prototype, but other known means not discovered the ways having similar characteristics. This allows to make a conclusion on the conformity of the proposed method the criterion of “substantial differences”.
The invention is illustrated by the following example.
Were selected for the experiment 50 cows of black-motley breed aged 2.5-5 years old. With the help of the device erpt-4M measured conductivity BAHT skin Teng-man. Depending on the readings of the cows were divided into three groups: 1st group - where the asymmetry between positive and negative polarity of the measurements is in the range from 0 to 2.5 µa, 2nd group - asymmetry from 2.5 to 5 μa, 3-I-asymmetry of more than 5 µa.
All experimental animals were observed during childbirth, was determined by their state in the postpartum period, the timing of joining the hunt after calving, fertility after insemination, the duration of the service period and the incidence of calves obtained from these cows.
All 25 cows assigned to the 1st group, complications were not observed. In 6 cases, the cows had complications in the postpartum period in the form of hypofunction of the ovaries, subinvolution of the uterus and persistent yellow body. 76% of cows came into the hunt in the first 30 days after calving, 72% of cows in this group, ill dyspepsia, mortality was not observed.
In the 2nd group of cows (13 goals) in two cases observed complications of childbirth - a dry birth, retention of placenta, spasms of the uterine cervix. In the postpartum period, 5 cows was identified subinvolution of the uterus, 3 - endometritis, 2 - persistent luteum in the ovaries and 3 - hypofunction of the ovaries. 62% cows come into heat for 30 days. After the first insemination were fertilized 62% of cows, after the second and third 38%. The duration of the service period up to 30 days was 46% of the cows. And at 8% for more than 90 days. 4 calves from cows in this group had dyspepsia.
In the 3rd group was observed complications of childbirth in 8 cows from 12. In 9 cases noted complications in the postpartum period. In the first 30 days after calving occurred to hunt 33% of cows in the period from 31 to 60 days after calving - 33% of cows. After the first insemination were fertilized only 33% of cows, 58% after the second and third insemination, and one cow were fertilized after the fourth insemination. Only 17 goals this group, the duration of the service period was less than 30 days, 17% of cows the service period was over 90 days. 4 calves born from cows of this group, ill dyspepsia and bronchopneumonia, 3 of them fell.
Cow 1 is crassocephalum.
Using the proposed method of determining the stress resistance of cows is easier in comparison with known methods, requires minimal time veterinary technician. To determine the stress resistance of one head of cattle spent 0.5 minutes, while for determining the value of milk yield and milking time (compared to the prototype) - 6-7 minutes. Stress cows when the claimed method is determined independently of the value of milk yield, the period after calving, stage of the sexual cycle.
SOURCES of INFORMATION
1. Furdui F. I. and other Stress and adaptation of farm animals in the conditions of industrial technologies. - Chisinau: Stinica, 1992 - 223 C.
2. Ustinov D. A. Stress factors in the cattle industry. - M.: Rosselchozizdat, 1976. - S. 140-145.
3. Kokorin E. P. guidelines for the assessment of stress tolerance cows during machine milking. - M.: Agropromizdat, 1986. - 245 C.
4. Plakhotin M. C. Acupuncture in veterinary medicine. - M.: Kolos, 1966. - 264 C.
The method of determining the stress resistance of cows by determining the condition of the nervous system functions of the body, characterized in that the device electroacupuncture PERT-4M determine boron in biologically active point skin Teng-man, responsible for the condition of the Central nervous system, and when the difference value is less than 2.5 μa diagnose stress, the 2.5-5 MCA - unstable stress, when the difference values greater than 5 μa - stressoustiychivost.