Drug for the treatment and prevention of endometritis and mastitis in cows and method of its preparation

 

(57) Abstract:

The invention relates to veterinary medicine, namely to veterinary gynecology. Drug for the treatment and prevention of endometritis and mastitis in cows is a ozonized cod liver oil. The method of producing drug is that after filtration of fish oil through him miss an ozone-oxygen mixture with ozone concentration of 10 or 20 mg/l for respectively 60 or 30 minutes. Therapeutic efficacy is 90-93,7%. 2 N. p. F.-ly, 5 PL.

The invention relates to veterinary medicine, namely to veterinary gynecology, and can be used as a therapeutic and preventive agent for endometritis and mastitis in cows.

Known officially approved 5-10% suspension of trichilia, 5% suspension furazolidone and 2.5% suspension furagin, cooked fish oil [1], used for the treatment of endometritis in cows.

The disadvantage of the above causal means is a narrow range of applications, low therapeutic efficacy and necessity rejection of milk after recovery cows due to accumulation of antibiotics and nitrofuranov organizing opnai therapy cows, patients with endometritis and mastitis, which is based on intrauterine and intracisternal the introduction of sediment furatsilina 1,0, furazolidone 0,5 and streptocide 3,5 respectively prepared on protein chicken eggs and milk. Additionally, the uterine cavity is entered 900-1000 ml of oxygen, and in the udder - 100-150 ml.

The disadvantage of this method of treatment is primarily its high cost, complexity of the process of preparation of a medicinal mixture, inadequate therapeutic efficacy, the need for rejection of milk during treatment and for 2 days after the disappearance of clinical signs of disease.

The proposed drug differs from the prototype in that it has antimicrobial properties, unidentifiable presence of antibiotics, sulfonamides, nitrofuranov, and the presence of ozonides-peroxides, resulting from the cleavage of double carbon bonds under the influence of ozone in unsaturated fatty acids.

unsaturated fatty acid ozonid peroxide

In the product number of ozonides of peroxides is from 500 to 2000. The drug also has the ability to stimulate the process of tissue regeneration. Based on the above, PR is apart is a clear, oily liquid straw or brown color with a specific smell of freshness. When stored in conditions below +4C may cause the bottom sediment gray color, which upon heating of the drug to a temperature of 20-25C disappears.

The method of preparation of the drug consists of passing through fish oil ozone-oxygen mixture with ozone concentration of 10 or 20 mg/l for respectively 60 or 30 minutes. Pre-fish oil is filtered through two layers of cheesecloth and pour into a special glass cylinder or bottle in 400 ml. For passing an ozone-oxygen mixture is used needle marks And 33, the end part which should touch the bottom of the glass vessel. The needle using a PVC tube connected to the ozonizer. The vial is closed with a stopper made of black rubber.

From the total mass of the prepared drug selected control sample for analysis and perform packing of dark glass with a capacity of 0.1...0.5 liters, sealed, eticaret and provide storage between +2-20S. The shelf life of the product is 6 months. The technological scheme of the preparation are shown in table 1.

For analysis of the finished product sample in 20 ml placed in a colourless about the lighting. The drug should be a lot of oily consistency from straw to brown color with a smell of freshness.

For monitoring to determine the authenticity of acid, peroxide value and fatty acid composition.

Determination of acid number (HT) is carried out in a conical flask, which is placed 30.01 g of the drug and poured 50 ml of neutralized ether-alcohol mixture and shaken. Add 3-5 drops of 1% alcoholic solution of phenolphthalein. The resulting solution with constant shaking rapidly titrate with 0.1 N. a solution of caustic potash or caustic soda until a distinct pink colour does not disappear within 1 minute. Acid number (X) is calculated by the formula:

where Y is the amount of 0.1 n alkali solution used for titration, ml; - amendment to terms of accurate to 0.1 n alkali solution; M is the mass of the sample of the study drug, g; 5,61 - the number of mg of sodium hydroxide contained in 1 ml of 0.1 n KOH solution. HT should be 1,60,1.

Peroxide value (FC) is determined by placement in a conical flask with a glass stopper 0,80.0002 g of the drug. Poured 10 ml of chloroform and 10 ml of glacial acetic acid. Quickly add 0.5 ml of saturated sugarholics and put in a dark place for 3 minutes. Then pour into the flask 100 ml of distilled water, in which pre-add 1 ml of 1% starch solution. Titrate with a 0.01 n sodium hyposulphite to the disappearance of blue color. FC (X) is determined by the formula:

where Y is the number of 0.01 n solution of hyposulphite of sodium, used for titration of the sample with the drug, ml; Y1- the amount of 0.01 n solution of hyposulphite of sodium consumed in the titration of a control sample, ml; M is the mass of the sample preparation, g; K - correction factor for conversion to accurate to 0.01 N. of hyposulphite of sodium. If the drug must be 948,4.

Determination of fatty acid composition is carried out according to GOST 30418-96 vegetable Oils (with the help of chromatograph. The content of fatty acids in the product should be as follows (%): myristic - 5,080,27; pentadactyla - 0,40,04; palmitic - 11,20,49; stearic - 1,60,07; oleic - 25,41,11; linoleic - 2,40,1; linolenic - 0,60,03.

To establish the optimal single dose of the drug it into the uterus of patients with endometritis cows were injected in a volume of 20, 25, 50 and 75 cm3and of mastitis in the cavity of the breast dose 5, 8, 10, 15, 20, 25 Jr. the Most high therapeutic effect with regard to economic calculations in the treatment of endometritis by pollinterval introduction 12-24 hours.

The results of the scientific-production testing of the drug in the treatment of endometritis and mastitis in cows is presented in tables 2 and 3.

Thus, therapeutic efficacy of the drug in endometritis and mastitis is higher in comparison with 10% suspension of trichilia fish oil and Matilda.

The efficacy of the drug for prevention of endometritis and mastitis in cows are presented respectively in tables 4 and 5.

Thus, the preventive efficacy against postpartum endometritis in cows is 90%, and in the prevention of mastitis in the dry period of 91.6%.

When the above-mentioned pathology in the reproductive organs and mammary gland in cows preparation before the introduction should be warmed up to a temperature of +38-40C to install into the uterine cavity rektotservikal way in a dose of 25-50 ml with an interval of injection 48 hours, and in the channel of the nipple of the affected quarter of the volume of 10-20 ml

Prevention of endometritis of Azonal RJ in the uterus in cows administered within the first 24 hours after calving dose of 50 ml, and for the prevention of mastitis drug is installed in each udder quarter at a dose of 5 ml per 4-5 de is actica and treatment of gynecological diseases of cows. M: Rosagropromizdat. - 1991. - S. 121.

2. Khilkevich N. M. Prevention and treatment of mastitis // veterinary medicine. - 1987. No. 4. - S. 53 - a prototype.

1. Drug for the treatment and prevention of endometritis and mastitis in cows containing an antimicrobial substance, characterized in that as antimicrobial substances contains fish oil, ozonated subjected ozone-oxygen mixture with ozone concentration of 10 or 20 mg/l for respectively 60 or 30 minutes

2. The method of producing drug for the treatment and prevention of endometritis and mastitis in cows of fish oil, including filter, characterized in that after filtering fish oil ozoniruyut transmission through it of ozone-oxygen mixture with ozone concentration of 10 or 20 mg/l for respectively 60 or 30 minutes

 

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