Method for the diagnosis of subclinical endometritis in cows

 

The invention relates to animal husbandry. The functional state of the uterus is determined by measuring the conductivity of biologically active points # 1 on the negative and positive polarity, calculate the coefficient of asymmetry of the conductivity and its value is 0.64-0.76 to, diagnosed with subclinical endometritis. The method allows you to quickly and objectively diagnose subclinical endometritis in cows. 1 Il., 1 PL.

The invention relates to livestock, in particular cattle, and can be used for the diagnosis of subclinical endometritis in cows.

Known methods of diagnosis of subclinical endometritis in cows: a study of cervical mucus K. C. Dudenko, the study of the estrous mucus, N. Kalinowska and G. L. Podoprigora, histological research method with endometrial biopsies, colored sedimentary reaction of urine by J. A. Kimbarovsky in the modification of MT Drach, as well as identifying the viability of sperm in cervical mucus I. A. Phlegmatism. [1]

The disadvantages of these methods include:

- the necessity of using expensive reagents;

- mandatory laboratory about aetsa exception to the use of expensive reagents, the use of sophisticated technological tools and laboratory equipment, as well as reducing the complexity and time consuming to research.

The problem is solved due to the fact that in the known method, which consists in the examination of the functional state of the uterus, according to the invention the functional state of the uterus is determined by measuring the conductivity of biologically active points # 1 on the negative and positive polarity, calculate the coefficient of asymmetry of the conductivity and its value is 0.64-0.76 to, diagnosed with subclinical endometritis.

The cow biologically active point (BAHT) No. 1 (see the drawing) is located at the intersection of the line connecting the sacral (inner) thigh wing of the Ilium with the buttock and the vertical line passing through a large skewer (BAT No. 1 are symmetrical).

To measure the asymmetry of the conductivity BAHT cows can be used by any device intended for carrying out of electroacupuncture, for example devices PERT-4m, PERT-5.

Before operation it is necessary to close the output electrodes “+” and “-” between themselves and handle the “current” set on the microammeter the current value equal to 20 MK is permetre. After finding BAHT to diagnose her condition by switching the toggle switch “polarity” on the positive and negative polarities, the obtained parameters substituted into the formula and calculate the coefficient of conductivity. When the value of the coefficient of electrical conductivity from 0.64 to 0.76 diagnosed with subclinical endometritis.

For fixation of the electrodes with suction cups, they must be connected to a vacuum milking system.

In the process, it is necessary to monitor the occurrence of contact of the electrodes with the body of the animal.

In our experiments we used 250 cows of Simmental and black-and-white breeds at the age of 3-5 years with a live weight of 360-500 and the average milk yield for 305 days of lactation 5400 kg

Example 1. The cow after the diagnosis of subclinical endometritis by the method of C. C. Dudenko was a positive reaction that characterize subclinical endometritis. In parallel physico-chemical study was conducted measuring the electrical conductivity of the positive and negative polarities in the BATH No. 1 instrument erpt-5 and calculated the coefficient of asymmetry of the conductivity A, which displays the ratio of conductivity to the negative polarity in the MCA “N” this indicator is positively Inoi polarity was equal to 250 µa, and on the negative polarity 175 µa, substituting these data into the formula, we obtained the coefficient of asymmetry of the conductivity was equal to 0.7. Other diseases are not detected.

Example 2. The cow after the diagnosis of subclinical endometritis by the method of C. C. Dudenko was a positive reaction that characterize subclinical endometritis. In parallel physico-chemical study was conducted measuring the electrical conductivity of the positive and negative polarities in the BATH No. 1 instrument erpt-5 and calculated the coefficient of asymmetry of the conductivity (A), which displays the ratio of conductivity to the negative polarity in µa (N) to this indicator for a positive polarity in µa (n) and is expressed by the formula: A=N/n. This cow the conductivity in the positive polarity was equal to 250 μa, and the negative polarity 160 µa, substituting these data into the formula, we received the asymmetry coefficient of the conductivity, is 0.64. Other diseases are not detected.

Example 3. The cow after the diagnosis of subclinical endometritis by the method of C. C. Dudenko was a positive reaction that characterize subclinical endometritis. Parallel to fistmaster in BATH No. 1 instrument erpt-5 and calculated the coefficient of asymmetry of the conductivity (A), which displays the ratio of conductivity to the negative polarity in µa (N) to this indicator for a positive polarity in µa (n) and is expressed by the formula A=N/n. This cow the conductivity in the positive polarity was equal to 250 μa, and the negative polarity 190 µa, substituting these data into the formula, we obtained the coefficient of asymmetry of conductivity equal 0,76. Other diseases are not detected.

The results of the measurement of the coefficient of electrical conductivity in a biologically active point No. 1 in the experimental cows are presented in table where you can see that when the value of the coefficient of conductivity of 0.68-0.76 to fix the disease subclinical endometritis.

The method allows you to quickly and objectively in quantitatively comparable values to diagnose subclinical endometritis in cows, this eliminates the use of expensive reagents, the use of sophisticated technological tools and laboratory equipment, which significantly reduces the economic costs of diagnostic tests.

The source of information

1. Comprehensive ecologically safe system of veterinary animal health. - M.: Moscow, Reintermediate is about the diagnosis of subclinical endometritis in cows, consists in the examination of the functional state of the uterus, characterized in that the functional state of the uterus is determined by measuring the conductivity of biologically active points # 1 on the negative and positive polarities, calculate the coefficient of asymmetry of the conductivity and its value is 0.64-0.76 to, diagnosed with subclinical endometritis.

 

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