The method of producing drug for conducting human patch test

 

(57) Abstract:

The invention relates to the production of drugs for research in the field of Allergology and can be used in dermatology to determine the cause of contact allergic reactions and irritation reactions in dental practice for determining sensitivity to an orthopedic ingredients and medicines, surgery and traumatology at the use of synthetic grafts in cosmetology to identify portability cosmetic and ointment preparations and their components, in pathology to determine the contact dermalive different nature. The essence of the invention lies in the fact that they are carrying out the mixing of a test substance with a carrier and a solvent as a carrier injected pre-melted white petrolatum, and as a solvent a mixture containing by weight distilled water in the amount of 75-82%, the acetone in the amount of 15-20%, ethanol in the amount of 3-5%, white petrolatum in g and the solvent (ml) taken in a ratio of 100:11, and the test substance, the carrier and the solvent is stirred for 7-10 min to obtain a homogeneous mass. Technical cut the th range of chemicals and susceptibility to the tested chemicals in the required concentrations, depending on the immunoreactivity of human rights. 1 C.p. f-crystals.

The invention relates to the field of biotechnology, namely to obtain drugs for research in the field of Allergology, and can be used in dermatology to determine the cause of contact allergic reactions and irritation reactions in dental practice for determining sensitivity to an orthopedic ingredients and medicines, surgery and traumatology at the use of synthetic grafts in cosmetology to identify portability cosmetic and ointment preparations and their components, in pathology to determine contact dermatitis of different nature.

The known method for non-invasive diagnosis of sensitization to chemical substances. As the study material used secret lining of the nose. Pre nasal mucosa treated with hematoxilin. Then on the mucous one half of the nose impose experienced nitrocellulose substrate with adsorbed on the allergen and the mucous second half of the control nitrocellulose substrate without allergen. After exposure each substrate is placed in a saline solution, incubated, remove the substrate from experienced and counter the noglobulin E (IgE) and to increase the level of these indicators in 1,5 times more than in the experimental sample relative to the control of histamine in the control sample is relatively experienced to diagnose IgE sensitisation of the body to the allergen (EN 2185630, G 01 N 33/536, 2002).

However, the known method is quite complicated for the diagnosis of sensitization to chemical substances. Requires the use of expensive equipment, the use of expensive reagents and highly qualified personnel.

The technical result of the claimed invention is the versatility of the method of obtaining the drug, allowing to define the contact allergic reactions and reactions of irritation for a wide range of chemicals, and to determine the sensitivity to these named test chemicals in the required concentrations, depending on the immunoreactivity of man.

This result is achieved in that the method of producing drug for conducting human patch test involves mixing a test substance with a carrier and a solvent as a carrier injected pre-melted white petrolatum, and as a solvent a mixture containing by weight distilled water in the amount of 75-82%, the acetone in the amount of 15-20%, ethanol in the amount of 3-5%, white petrolatum in g and the solvent (ml) taken in a ratio of 100:11, and the test substance, wear the ve test substance using chemical substances, can cause contact allergic reactions or reactions of irritation, and take them in concentrations of from 0.05% to 70%.

The invention is illustrated in the following examples.

Example 1. The preparation for the detection of delayed-type hypersensitivity to Nickel ions (in any field of Allergy, such as surgery, dermatology, dentistry, pathology).

Take the following ingredients:

white vaseline;

- a mixture of acetone and ethyl alcohol in the ratio of 4:1;

- distilled water;

Nickel sulfate hydrated as the test substance.

Heat 10 g of white vaseline to a liquid state to a temperature 581C. Dissolve 0.5 g of Nickel sulfate in 0,85 ml of distilled water by heating to a temperature not exceeding 50C. Mix melted white petrolatum with an aqueous solution of Nickel sulfate. Produce a slow stirring for 5 minutes at room temperature. Then gradually add the pre-cooked mixture of acetone (80%) with ethyl alcohol (20%) in an amount of 0.25 ml and mixing lead to a smooth homogeneous mass within 10 minutes.

Example 2. Preheat the following ingredients:

white vaseline;

a mixture of distilled water, acetone and ethyl alcohol in the ratio 17:4:1 (77,27%: 18,18%: 4,55%);

cream-gel for hair coloring “Recolor” (Russia) as the test substance.

Heat 1 g of white vaseline to a temperature not exceeding 50°C. Gradually without heat gain of 0.11 ml of a mixture of acetone, ethanol and distilled water. To the resulting mass was added 0.5 g of cream-gel at room temperature and constant stirring for 7 minutes

Example 3. The preparation for determination of sensitivity to the drugs used in dental practice.

Take the following ingredients:

white vaseline;

a mixture of distilled water, acetone and ethyl alcohol in the ratio 17:4:1 (77,27%: 18,18%: 4,55%);

sealing material 3M Valux plus A2 Shade 5540 A2” as the test substance.

Heat 1 g of white vaseline to a temperature not exceeding 50°C. Gradually without heat gain of 0.11 ml of a mixture of acetone, ethanol and distilled water. To the resulting weight gain of 2.33 g of filling material (70%) with constant stirring for 7 min prior to the formation of homogeneous preparation.

The cell is attached to the skin with adhesive tape to ensure full contact of the drug over the entire area of the cell with the skin. After 48 hours remove the cell with the drug. Initial inspection of the contact areas spend 24 hours after removal of the cell.

In case of detection of the contact area of hyperemia, itching, papular rash, education infiltrate, concludes a positive result.

If during the primary inspection, the contact remains unchanged, then the monitoring continues even within 2-4 weeks, and if no changes, then make a conclusion about the negative result.

The claimed method is versatile because it allows you to define the contact allergic reactions and reactions of irritation for a wide range of chemicals and to determine the sensitivity to these named test chemicals in the required concentrations, depending on the immunoreactivity of human rights.

1. The method of producing drug for conducting human patch test, comprising mixing a test substance with a carrier and a solvent as a carrier injected pre-melted white petrolatum, and as a solvent a mixture, Sodeistvie 3-5%, this white petrolatum in g and the solvent (ml) taken in a ratio of 100:11, and the test substance, the carrier and the solvent is stirred for 7-10 min to obtain a homogeneous mass.

2. The method according to p. 1, characterized in that as a test substance using chemical substances that can cause contact allergic reactions or reactions of irritation, and take them in concentrations from 0.05 to 70%.

 

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SUBSTANCE: invention proposes a method for preparing allergenic preparation used for diagnosis of body radiation injures. Method involves irradiation of potato tubers by the dose 350-400 Gr, the following extraction of quinoid radiotoxin by extraction with ethanol followed by removing extractant in rotary evaporator and additional extraction with ethyl acetate. The final prepared fraction is subjected for chromatography and separated in the system: (a) 2% acetic acid, and (b) mixture benzene - acetic acid - water in the ratio = 2:4:1, respectively. The prepared allergenic fraction is eluted with 2% acetic acid solution and standardized by dry matter as measured for 1 mg/cm3. Also, invention proposes a method for diagnosis of body radiation injures involving intracutaneous administration of specific allergen and detection of autosensitization symptom. Diagnosis of radiation diseases is proved by the allergy index. Proposed methods provide preparing the specific radiation allergen for detection of radiosensitization of body and to carry out diagnosis of radiation disease. Invention can be used in radiation biology.

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1 tbl, 3 ex

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SUBSTANCE: allergen is taken as aqueous salt solution of protein polysaccharide complex of 10000 PNU activity produced by extracting dry degreased gnat of Aedes aegypti with alkaline buffer solution at pH of 8.0-8.2 with following cleaning as means for specific immunotherapy means applicable to allergic disease patients having sensitization to gnat stings. Method involves applying allergen of 10000 PNU concentration in 10-fold dilution introduced to a patient beginning from a dose of 0.2 ml with 1-2 days long pause. Supporting doses of the allergen are administered to the patients before gnat flight season with 10-12 days long pauses.

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EFFECT: higher accuracy of prediction and improved quality of therapy.

3 ex

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1 ex

FIELD: veterinary microbiology.

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2 ex, 2 tbl

FIELD: medicine.

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EFFECT: invention provides producing high effective treatment allergen in simplified process technology.

2 ex

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