Aqueous germicidal composition for the prevention and treatment of infectious diseases of the hoof in animals and method of prevention and treatment of infectious diseases of the hoof in animals

 

(57) Abstract:

The invention relates to the field of veterinary medicine. Water-based composition of salts of copper includes the additional Quaternary ammonium compounds and peroxide in the following ratio, wt.%: salt of copper 2-20; Quaternary ammonium compounds of 0.5-2; peroxide of 0.5-5; water the rest. The method consists of local application of an effective amount of aqueous germicidal composition. Composition and method allow to increase the effectiveness of treatment and to reduce the period of time. 2 C. and 15 C.p. f-crystals, 6 PL.

This invention relates to the treatment and prevention of infectious diseases of the hoof in animals.

Infectious diseases of the hoof, such as obmolochennie ungulates warts (papillomatosis digital dermatitis, or "SDA"), hoof rot (interdigital cellulitis) and stable hoof rot (interdigital dermatitis), common in farm animals such as sheep, goats, horses, cattle dairy and beef breeds.

Disease of ungulates warts was first recorded in Italy in 1974 and has since spread around the world. Since the beginning of 1980-ies ungulates warts are common preciousest. For example, a recent study of the Ministry of agriculture of the USA showed that 47% of dairy cattle in the United States affected the SDA, and 78% of infected animals is the first case of the disease was observed in 1993 or later (Digital Dermatitis on US. Dairy Operations, NAHMS Dairy Study, May, 1997).

The clinical expression of the SDA is the appearance of limp, varying in extent, within a given herd animals. This is a superficial skin disease fingertips animals that manifest differently, depending on the stage of the lesion: the painful, moist, resembling a berry strawberry, up high, oboronnyh, Borodavkin formations. In the absence of proper treatment it can lead to severe lameness and even death. In dairy cattle ungulates warts cause reduced milk yield, fertility and body weight.

Although initially it was believed that traffic is caused by a virus, it is now believed that it is caused by one or more species of bacteria. The researchers identified from multiple injuries caused by the MVT, two different species of spirochaetes, but failed when using pure cultures of these organisms can cause infectious disease in healthy animals, thus showing that for Vozniknovenie, or interdigitale cellulitis, infection soft tissue between the toes. In horses it is also known as the decay of the arrow hooves. Further, the term "hoof rot" refers to both the actual hoof rot and decay of the arrow. Hoof rot is caused by an anaerobic bacterium Fusobacterium necropborum. In the development of the disease involves the anaerobic Dicbelobacter (Bacteriodes) nodosus and Prevotella melaninogenicus. The bacteria infect the skin of the feet on the damaged areas and initially cause a painful swelling of the skin between the toes. Then along the swollen area on part or on entire interdigitating space develops groove or crack. Without treatment, hoof rot can affect the joints, bones and/or tendons of the feet, which makes recovery unlikely. Animals affected hoof rot, there has been little fever, loss of appetite and associated weight loss. Develops lameness varying degrees of severity.

Interdigital dermatitis, or stable hoof rot, is usually a chronic inflammation of the skin between the toes (interdigital cracks). This infection is caused by the bacterium Dichelobacter nodosus. The skin in the area interdigitale cracks lameness or cracking of the heel part of the hoof or erosion, but usually leads to changes in the gait of the animal.

Currently ungulates warts, foot rot and stable hoof rot treated in several ways. The most effective method of treatment is the use of antibiotics, such as tetracycline, lincomycin, spectinomycin, ampicillin, oxytetracycline and ampicillin, which are delivered to the affected areas using foot baths, aerosol spray or wraps feet (Britt J. S., J. Gaska, E. F. Garrett, D. Konkle and M. Mealy. 1996. Comparison of the local application of three products for the treatment of PPD in dairy cattle. J. Am.Vet.Assoc. 209:1134).

Although antibiotics are effective in the treatment of these infectious diseases, their use has certain disadvantages. Antibiotics road, and there is a danger that their use, especially in the treatment of dairy cows, can cause the presence of antibiotic residues in meat and milk of animals. In addition, widespread use of antibiotics may cause the emergence of resistant to antibiotics strain of bacteria. Finally, at the present time, the use of antibiotics for the treatment of foot rot, foot rot resistant or SDA is not recommended, because antibiotics are not designed for this specific purpose.

As sredstvami also germicide chemical based. Although some germicide, for example, containing copper sulfate and zinc sulfate, have a significant effect in foot rot and foot rot resistant, they are ineffective in the case of ungulates warts. We also used Quaternary ammonium compounds, but their effectiveness in the treatment of PDD has never been proven. In addition, these substances are ineffective in large breeding such, what happens in foot baths, and many of them are expensive. Similarly used a combination of hydrogen peroxide with peracetic acid, but they are also ineffective against traffic, unstable and poorly stored. In addition, these mixtures in concentrations recommended for treatment, irritated hoof (Shearer J. K. Strategies for treatment and control of digital dermatitis (obmolochennie ungulates warts). Proceedings of the conference on the health of the hooves, 1997, Batavia, new York).

Appeared inaccurate reports about the successful use of formaldehyde for the treatment of traffic, but controlled trials showed that formaldehyde is less effective than antibiotics. In addition, formaldehyde refers to the carcinogens and toxic substances that in some areas of the United States its use is prohibited. Further, the use of formaldehyde in too high the zoom, the use of formaldehyde for the treatment of foot rot, foot rot resistant and traffic is impractical and inefficient.

As mentioned above, for the prevention of foot rot, foot rot resistant and traffic usually try to use a foot bath with germicide, such as copper sulfate, hydrogen peroxide or even antibiotics. Typically, foot baths are diluted compositions used in aerosols and wraps. However, the effect of herbicide or antibiotic included in this composition, can be easily negated harsh environmental conditions associated with baths. In the baths become a suitable environment for bacteria and may not actually be prevented, and to accelerate the spread of infectious diseases of the hoof (Hemandes J., Shearer J. K. and J. B. Elliott, 1999. Comparison of topical application of oxytetracycline and four do not contain antibiotics solutions to ensure papillomatoses digital dermatitis in dairy cows. J. Am.Vet.Assoc. 214:688).

Thus, there is a need for bactericidal composition, which would be effective against foot rot, foot rot resistant and traffic, affordable and would avoid the use of antibiotics. is DD. In addition, there is a need for bactericidal composition which can be effectively used in foot baths and to withstand the harsh conditions associated with their use. These needs are answered by the composition and method which is the subject of this invention.

Unexpectedly, it was found that aqueous solutions of microbicides (germicidal) containing a salt of copper, Quaternary ammonium compounds and peroxide, are effective for the treatment and prevention of traffic, despite the fact that none of these germicidal separately not effective against MVT in any concentration. Moreover, although none of these germicidal separately not effective against traffic in field trials on dairy cattle affected traffic, the above composition was as effective in the treatment of PDD as antibiotics. In addition, the composition proposed in the present invention, more effective against foot rot and foot rot resistant than any of its constituent substances separately, and better withstand adverse conditions footbath. Finally, the present invention also provides a method of treatment of infectious diseases of the hoof in animals, it provides a systematic way to apply an effective amount washeteria, effective in a wide range of conditions and dilutions, inexpensive and does not have any of the disadvantages associated with the use of antibiotics.

Here is the preferred embodiment of the invention.

Salt of copper

Salt of copper, proposed by this invention can be any salt of copper, soluble in water, such as copper sulfate, copper benzoate, bicarbonate of copper, copper nitrate, nitrite, copper, copper chloride, copper acetate, copper formate, trichloracetic copper, copper citrate, copper gluconate, and mixtures thereof. Can be used and other salts of copper, provided an acceptable biocides anion and solubility of copper cations in the water. Preferred for use in the proposed composition and method copper salt is copper sulfate.

Salt of copper must be present in an amount from about 2% weight of the composition up to that number, when the solution is saturated, and more salt copper cannot be dissolved. In the case of copper sulphate solubility of approximately 20 weight percent of copper sulfate to the total weight of the composition. Preferably the presence of salt in the amount of from about 15% to 20% weight of the composition, where the range includes both 15% and 20% of the salt of copper.

Preferred Quaternary compound ammonium described in this invention the composition is a mixture of chloride N-alkyl(C12-18)-N,N-dimethylbenzylamine and chloride N-alkyl(C12-18)-N,N-dimethylethylenediamine, sold under the trade name BTC 2125 M offered by Stepan company (Northfield, Illinois). Other preferred Quaternary compound ammonium described in this invention the composition is a mixture of chloride N-alkyl(C12-18)-N,N-dimethyl-N-benzylamine, chloride N-dodecyl-N,N-dimethyl-N-ethylbenzylamine and chloride N-tetradecyl-N,N-dimethyl-N-benzylamine, sold under the trade name Barquat 4280-2 the ome 0.5 to about 2% by weight aqueous solution. Preferably the presence of Quaternary ammonium compounds in an amount of about 1 to 2% of the weight of the composition.

Peroxide

The composition and the method proposed by this invention can be any water-soluble peroxide. The preferred peroxide is hydrogen peroxide; can be either concentrated or dilute aqueous solutions of hydrogen peroxide. The initial peroxide content should be from about 0.5 to about 5% by weight aqueous solution described by this invention. Over time, the content of peroxide in the composition is slowly reduced due to its decomposition. Preferably the presence of hydrogen peroxide in an amount of about 1 to 2% of the weight of the composition described in this invention.

The compositions described by this invention should have a pH sufficient for complete dissolution of the salts of copper. the pH of the composition, thus, should be about 3 or less, preferably from about 1.6 to about a 2.0. Aqueous germicidal composition must also be stable under normal storage conditions. The viscosity of the composition can vary depending on its intended use and can be optionally povyshenie, you can also enter other components. For example, to bring the pH of the composition to the desired values can be used pH regulators. For this purpose, can be used as mineral and organic acids and mixtures thereof. Among the usable organic acids include hydroxyestra acid, citric acid and lactic acid, while from inorganic acids suitable phosphoric acid, sulfuric acid and hydrochloric acid. It is preferable to use an organic acid or mixture of organic and mineral acids, since it is believed that the peroxide can be formed with an organic acid biocide "percolate". The amount of pH regulator depends on the desired pH. Typically, the pH regulator must be present in an amount of from about 0.5 to 2.0% by weight of the composition.

Water compositions described in this invention can also contain a fixative pH buffer, ensuring that all components of the composition will remain soluble throughout the shelf life of the composition. Can be used organic bases, such as amines and inorganic bases such as sodium hydroxide and potassium hydroxide. Usually in the morning and from specific components of the composition.

The composition described in this invention may also contain other additives, which can be any substance that enhances the properties of the compositions in the aspect (i) improve the solubility or dispersion properties of the other components, (ii) improve the adhesion of the composition to the affected area of the hoof, (iii) adjustment of performance wetting and (iv) improve the stability, which may be related, among other factors, with characteristics such as surface tension and viscosity. The composition described in this invention may also contain dyes, providing visibility of the applied composition to ensure that it is correct and complete application. In addition, the composition may contain other substances, such as softening means of reducing skin irritation with local application.

Method of treatment of diseases.

This invention related to a method of prevention or treatment of infectious diseases of the hoof in animals, is the local application of the claimed aqueous composition to an affected area or region affected area. It is preferable to use the composition for the treatment of PDD. The composition can be applied by pouring, VP is STCA either by inclusion in the composition of funds to wrap the feet. The best option is the application of this composition by spraying. Alternative treatment options is wetting or immersion hoofs of the animal in the claimed composition.

To fight or prevent traffic, foot rot and foot rot resistant composition can be used in foot baths through which the animals. Generally, the composition should be diluted with additional water; however, the composition should not be diluted to the point where its bactericidal activity can be quite easily suppressed harsh environmental conditions that affect the foot bath, such as the presence in the bath of faeces. For use in a foot bath of the composition described in this invention can be diluted with water to 1000 parts. Preferably 200-fold dilution.

A composition

Aqueous germicidal composition described in this invention can be manufactured in the usual way. Preferably Quaternary ammonium compounds before the introduction of the salt of copper. Peroxide should not be entered until the salt is completely dissolved copper. After the introduction of all components of the composition of shadetolerant.

The present invention is illustrated by the following examples. These examples demonstrate various aqueous germicidal compositions and methods included in the content of the present invention, which cannot be interpreted as limits that restrict the content of the invention.

EXAMPLE 1

The water composition in example 1 was prepared by mixing the components in the following order:

Material: Weight%:

Water 71,9

Hydroxyestra acid 0,5

rl 12(SAS) 1,0

MTC 212 5M (Quaternary) 1,0

75% phosphoric acid 0,6

The copper sulfate pytevodniy 20,0

Hydrogen peroxide 5,0

Just 100

The above composition had a pH in the range from 1.6 to 2.0 and a viscosity of 7.0 centipoise.

EXAMPLE 2

The water composition in example 2 was prepared by mixing the components in the following order:

Material: Weight%:

Water 76,1

Natrosol 250 MR-CS 0,2

50%NaOH 0,1

Barquat4280-Z 1,0

Barlox 12,30% 1,0

Hydroxyestra acid 0,5

75% phosphoric acid 1,0

The copper sulfate pytevodniy 15,0

35% hydrogen peroxide 5,0

FD&C blue No. 1 0,1

Total: 100

The above composition had a pH in the range from 1.6 to 2.0, a viscosity of 4-6 Sant who stayed in the field for cows, the affected traffic. Participated in experiment 66 cows, sick SDA varying severity. The animals involved in the experiment, were divided into 6 groups; the first group was treated by the claimed composition, the composition of which is given in example 1, the remaining group of cows treated with other drugs, commercially available, and as a positive control was used a group of 5 cows that were treated with oxytetracycline. As a negative control was used a group of 6 cows that were treated with water.

Before treatment for each cow were set the size of the damage, location of damage, the appearance of the damage, the degree of development of dermatitis and severe pain. The composition was sprayed on the affected areas of the hoof during the first week and once on Monday, Wednesday and Friday of the second week. Within two weeks of treatment and approximately two weeks after the end of each animal were examined and evaluated on the above indicators. Then based on the assessment of lesion size was determined by the improved condition of the affected area, in percent, the level of pain when positive the neck improvement, the less positive change. The research results are shown below in table.I and table.II.

The results show that the composition of example 1 was substantially more effective for the treatment of PDD than sravnivayete with her songs, except for oxytetracycline. Compared to oxytetracycline composition of example 1 was equally effective for the treatment of PDD.

EXAMPLE 4

The compositions of examples 1 and 2 were tested in field conditions in cows affected traffic. In total, 50 cows, sick SDA varying severity treated with either sample 1 or sample 2, or oxytetracycline, or a composition containing 27.5% hydrogen peroxide and 5.8% peracetic acid, or water. Before treatment for each cow was established lesion size, color damage and the level of pain, which was calculated the total extent of damage. The degree of damage was calculated by prisvaivaetsya numeric indicators for lesion size (0-2), color damage (1-4) and level of pain (0-2), with a higher score indicating a more severe condition. Then the cows were divided into experimental gr the TCI hooves once daily for the first week and once for four consecutive days the second week. After two weeks, the treatment was stopped, and the cows were evaluated on the above indicators. For each experimental group was calculated the average degree of damage, the percentage improvement was determined by subtracting the average degree of damage after treatment of moderate damage before treatment, and dividing the result by the average degree of damage before treatment. The results are shown below in table.III and table.IV.

The results show that the compositions of example 1 and example 2 were equally effective for the treatment of PDD, as oxytetracyclin, and significantly more effective than hydrogen peroxide and peracetic acid or the negative control (water).

The composition of example 1 was tested to determine its effectiveness compared to commercially available compositions when exposed to Fusobacterium necrophum, the bacterium that causes foot rot. The song was first tested undiluted against pre-determined concentrations of bacteria in test tubes, and plates. Then the composition was used in increasing dilutions until you reach the point when diluted composition continued to suppress bacterial growth. This new point number double dilutions, made before the composition is stopped to suppress bacterial growth. For example, the IPC equal to 4, meaning that it was made three double cultivation, so that the final dilution was 1:8. Thus, the dilution corresponding to the IPC, is defined by the formula 0=2N-1where D is the number of breeding, and N is the value of the IPC. As indicated above, the composition was tested in test tubes and plates. The tests in the test tubes was considered to be more accurate and standardized, while tests on the plates accurately mimic the conditions of high workload organics generated in foot baths.

The results of the actions on Fusobacterium necrophum are shown below in table.V. Composition with a higher IPC has when testing a stronger antibacterial effect than a composition with a lower value of the IPC. The number in parentheses indicates the number of times it was repeated a particular test.

EXAMPLE 6

The tests of example 4 were repeated in respect of Dichelobacter nodosus as the body, which is considered the causative agent of foot rot and foot rot resistant. The results are shown below in table.VI.

1. Aqueous germicidal composition for the prevention and treatment of infectious diseases of the hoof in animals I and peroxide in the following ratio of components, wt.%:

Salt of copper 2 - 20

Quaternary ammonium compounds of 0.5 - 2

Peroxide is 0.5 - 5

Water the Rest

2. The water composition under item 1, in which the pH of the composition is less than 3.

3. The water composition under item 1, in which the copper salt is 15-20% by weight of the composition, of Quaternary ammonium compounds is 1-2% by weight of the composition and the peroxide is 1-4% by weight of the composition.

4. The water composition under item 1, in which the copper salt is a copper sulphate.

5. The water composition under item 1, in which the Quaternary ammonium compounds represents at least one of the chlorides of trialkylborane, in which the benzyl group may be a Deputy representing ethylaniline radical.

6. The water composition under item 1, in which the peroxide is hydrogen peroxide.

7. The water composition under item 1, in which the copper sulfate is present in the amount of 15-20% by weight of the composition, of Quaternary ammonium compounds containing at least one of the chlorides of trialkylborane, in which the benzyl group may be a substituent in the form of ethylbenzene radical, present in the amount of 0.5-2% by weight of the composition and the hydrogen peroxide is present the play contains a pH regulator.

9. The water composition under item 8, which further comprises a latch pH.

10. The water composition under item 9, which further comprises a surfactant.

11. The water composition under item 10, which further comprises a thickener.

12. The method of prevention and treatment of infectious diseases of the hoof in animals, consisting of local application of an effective amount of aqueous germicidal composition according to any one of paragraphs.1-11.

13. The method according to p. 12, in which infectious disease is papillomatosis digital dermatitis.

14. The method according to p. 12, in which infectious disease is hoof rot.

15. The method according to p. 12, in which infectious disease is stable hoof rot.

16. The method according to p. 12, in which the coating composition is carried out by spraying.

17. The method according to p. 12, in which the coating composition is in the form of foot baths.

 

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