A method of obtaining a powdery material to stop bleeding

 

(57) Abstract:

The invention relates to the pharmacy, and in particular to methods of obtaining hemostatic drugs. A method of obtaining a powdery material having hemostatic action, provides for mixing in the water environment Foundation - partially oxidized cellulose with thrombin and fibrinogen, while to these substances additionally add gelatin, -aminocaproic acid and lysozyme, as well as partially oxidized cellulose use valdecaballeros in the form of fabric having a degree of oxidation, i.e., the content of aldehyde groups, from 4 to 6 %, with the following relations between the components: valdecaballeros 1 g; fibrinogen 18-22 mg; gelatin 27-33 mg; -aminocaproic acid 45-55 mg; lysozyme 9.5 to 10.5 mg; thrombin 350 units; water and 6.5 ml of Prepared solution of fibrinogen, -aminocaproic acid and half of the total amount of gelatin in half of the total amount of water and separately, a solution of thrombin and lysozyme and the remaining amount of gelatin in the remaining amount of water, the resulting solution was incubated for 3-4 hours at half the number of valdecaballeros, semi-drained, dried in air and podvergaetsia relates to pharmacy, specifically, to methods for hemostatic (hemostatic) drugs based on partially oxidized cellulose.

The above-mentioned drugs known. So, in the EPO application No. 1153620 described preventing bacterial infection in wounds hemostatic bandage from oxidized cellulose SURGICEL (firm “Johnson & Johnson”), containing from 1 to 10 000 ppm of iron ions (3+), and from EPO application No. 0468114 known soluble hemostatic material in the form of webs of cellulose subjected to the oxidation of monochloracetic acid and sodium hypochlorite for introducing carboxyl groups COOH.

Selected as the closest analogue of the EPO application No. 0659440 describes a method for hemostatic based on oxidized cellulose containing 0.5-4.0% calcium and thrombin, fibrinogen and/or angiofibromas. The disadvantages of this method include the use of cellulose, mild oxidation of which with the transformation of CH2Oh-groups in the carboxyl (COOH), it is not possible to obtain a material that provides the possibility of a strong link with him ingredients: only the ions of CA2+associated with COOH-groups of the ionic forces, while the remaining components of the first grinding, for example, in the powder.

The aim of the invention is to develop a method of obtaining a styptic substances on the basis of the partial oxidation of cellulose, which was devoid of the above disadvantages.

The goal has been achieved due to the fact that the method of obtaining compounds having hemostatic action, which involves mixing in the water environment Foundation - partially oxidized cellulose with coagulation factors, for example, thrombin and fibrinogen, to these substances additionally add gelatin, -aminocaproic acid and lysozyme, as well as partially oxidized cellulose use dialdehydes in the form of a fabric having a degree of oxidation, i.e., the content of aldehyde groups, from 4 to 6%, with the following relations between the components of:

Valdecaballeros 1 g

Fibrinogen 18-22 mg

Gelatin 27-33 mg

-Aminocaproic acid 45-55 mg

Lysozyme 9.5 to 10.5 mg

Thrombin 350 IU

Water and 6.5 ml

Components can withstand up to full covalent binding of drugs with dialdehydes with subsequent drying and grinding into powder. To prevent entries batch is on, -aminocaproic acid and half of the total amount of gelatin in half of the total amount of water and a solution of thrombin and lysozyme and the remaining amount of gelatin in the remaining amount of water, the resulting solution was incubated for 3-4 hours at half the number of valdecaballeros, semi-drained, dried in air and subjected to joint grinding. Additional introduction of gelatin-aminocaproic acid and lysozyme due to the following reasons: -aminocaproic acid, a substance inhibiting fibrinolysis. Blocking plasminogen activators and partially inhibiting the action of plasmin, aminocaproic acid may provide specific hemostatic effect for bleeding associated with increased fibrinolysis. Lysozyme has bacteriolytic action, has the ability to destroy microbial polysaccharides shell. Gelatin is a product of denaturation of the collagen under the action of heat or in the presence of salts formed during the destruction of the triple helix of collagen. Gel-like, water-soluble gelatin differs in that it contains a single-chain rich in lysine residues and shed (or hydroxytrol is ilenia 4-6% sufficient for covalent binding of all medicines with media (all components are available amino group and capable of forming with aldehyde groups azomethine linkage). The excess aldehyde groups leads to accelerated hydrolytic degradation of the drug that reduces its hemostatic activity. The disadvantage of not providing full chemical linkage that increases the time required to stop bleeding. The invention is illustrated by the example of its implementation.

Example 1.

Prepare a solution: 20 mg of fibrinogen, 15 mg of gelatin, 50 mg of aminocaproic acid 3.25 ml of distilled water, treated with a solution of 0.5 g of valdecaballeros in the form of cloth with a degree of oxidation of 5% for 3 hours and dried in air.

Prepare a solution of 350 IU of thrombin, 10 mg of lysozyme and 15 mg gelatin 3.25 ml of distilled water, treated with a solution of 0.5 g of valdecaballeros in the form of cloth with a degree of oxidation of 5% for 3 hours and dried in air. After drying cloths combine and grind into powder.

Example 2.

In experimental conditions in adult rabbits cause bleeding during intravenous injection of 500 IU of heparin and 250 DB fibrinolizina. Mechanically, the bleeding stopped after 20-30 minutes Under hypentelium anesthesia superficial excision of the fragment liver size 1.4 cm2caused the bleeding, the wound posip the b of obtaining the substance, having hemostatic action, providing mixing in the water environment Foundation - partially oxidized cellulose with coagulation factors, for example, thrombin and fibrinogen, wherein the specified substances additionally add gelatin, -aminocaproic acid and lysozyme, as well as partially oxidized cellulose use valdecaballeros in the form of fabric having a degree of oxidation, i.e., the content of aldehyde groups, from 4 to 6 %, with the following relations between the components of:

Valdecaballeros 1 g

Fibrinogen 18 - 22 mg

Gelatin 27 - 33 mg

-Aminocaproic acid 45 - 55 mg

Lysozyme 9.5 to 10.5 mg

Thrombin 350 units

Water and 6.5 ml

when separately prepared solution of fibrinogen, -aminocaproic acid and half of the total amount of gelatin in half of the total amount of water and a solution of thrombin and lysozyme and the remaining amount of gelatin in the remaining amount of water, the resulting solution was incubated for 3-4 hours and half the number of valdecaballeros, semi-drained, dried in air and subjected to joint grinding.

 

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