A method for the diagnosis of psoriasis

 

(57) Abstract:

The invention relates to medicine, in particular to skin diseases. The method allows for diagnosis of psoriasis in a short time with the least effort, and, in addition, it is retraumatized. Conduct a microscopic examination of biological tissue, thus taking a sample of blood serum at 20-25 µl, applied to the surface of the glass in the form of drops, dried for 18-24 hours at a temperature of 20-30C, and when detecting on the surface of the dried drops of the edge - amorphous zone, intermediate - unit, scattered subcourse structures and Central - with rounded structures of irregular shape with cavities and hollows, placed on the background of the amorphous surface of the Central zone, diagnose psoriasis. 2 Il.

The invention relates to medicine, namely to laboratory methods of skin diseases, and can be used in the diagnosis of psoriasis.

Known laboratory methods of diagnosis of psoriasis on immunomorphological picture (L. C. Beletskaya and other Labeled antibodies in normal and pathological morphology (Atlas), M, 2000, page 19).

The clinically is in the form of a flat papules pink-red color size up to 1 cm, potowatomi consistency, slightly above the level of healthy skin. For these lesions is characterized by the following diagnostic phenomena - psoriatic triad (the phenomenon of stearic spots, the phenomenon of psoriasis of the film, the phenomenon of blood dew).

The disadvantage of this method is the low reliability of the diagnosis, since it is based on a subjective assessment of a doctor.

The closest is a method of diagnosis of psoriasis, including microscopic examination of biological tissue (B. A., Berenbaum and other Differential diagnosis of skin diseases. - M, Medicine, 1989, page 106.

In this way the diagnosis of psoriasis is carried out by histological examination of a sample of the skin. This defines the characteristic morphological changes on the surface of the skin: parakeratosis, acanthosis, thinning malpighiales layer over the elongated papillae of the dermis. Typical lymphomacrophagal infiltration around blood vessels of the dermis.

The disadvantage of this method is its invasiveness, complexity, this study requires a considerable investment of time and expensive equipment.

The task was to remove otci time to diagnose psoriasis with the lowest labor costs.

The task is solved in that in the method for the diagnosis of psoriasis, including microscopic examination of biological tissue, take samples of blood serum at 20-25 µl, applied to the surface of the glass in the form of drops, dried for 18-24 h at a temperature of 20-30C, and when detecting on the surface of the dried drops, edge - amorphous zone, intermediate - unit, scattered subcourse structures and Central - with rounded structures of irregular shape with cavities and hollows, placed on the background of the amorphous surface of the Central zone, diagnose psoriasis.

In Fig.1 shows the appearance of the dried drops when the skin separatistskogo character (eczema), and Fig.2 - the same, in the presence of psoriasis.

The method is as follows.

The patient is taken into a test tube of blood, incubated for 2 h at a temperature of 20-30C. Then, the sample of blood serum is applied to the surface of glass slides in the amount of 20-25 µl, applied to the surface of the glass in the form of a drop and leave at a temperature of 20-30C to complete drying. Next viewing under low magnification (15) the whole surface of the drops and when the detection on the surface of the you is and and Central - with rounded structures of irregular shape with cavities and hollows, placed on the background of the amorphous surface of the Central zone, diagnose psoriasis.

Examples.

1. Patient S., 1955 R., case history No. 1321. Diagnosis at admission-exacerbation of chronic eczema. We took a sample of blood in quantities of 25 µl, applied as drops on the surface of the glass. Dried droplet has a different background color, clear and rich structuring, located in the Central area of the drops, and in the transition and peripheral zones, with numerous cracks, nuclei, nuclear formations, waves of chemical activity. Also detected the presence of pathological structures in the form of Arnold tongues in transition and marginal zones (Fig.1).

Conclusion: skin lesion separatistskogo character.

2. Patient P., 1953 R., case history No. 13804. Diagnosis at admission chronic eczema. When using the microscope on the surface of the dried drops identified: regional - amorphous area, intermediate - unit, scattered subcourse structures and Central - with rounded structures of irregular shape with cavities and grooves similar to “geographic patterns”, which is pretty psoriasis.

The method is technically simple. It can be used to diagnose psoriasis with unclear clinical picture without histological and histochemical studies.

A method for the diagnosis of psoriasis, including microscopic examination of biological tissue, wherein the taking a sample of blood serum at 20-25 µl, applied to the surface of the glass in the form of drops, dried for 18-24 hours at a temperature of 20-30C and identifying on the surface of the dried drops of the edge - amorphous zone, intermediate - unit scattered subcourse structures and Central - with rounded structures of irregular shape with cavities and hollows, placed on the background of the amorphous surface of the Central zone, diagnose psoriasis.

 

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FIELD: medicine, clinical toxicology.

SUBSTANCE: at patient's hospitalization one should gather the data of clinical and laboratory values: on the type of chemical substance, patient's age, data of clinical survey and laboratory values: body temperature, the presence or absence of dysphonia, oliguria being below 30 ml/h, hemoglobinuria, erythrocytic hemolysis, exotoxic shock, glucose level in blood, fibrinogen and creatinine concentration in blood serum, general bilirubin, prothrombin index (PTI), Ph-plasma, the state of blood clotting system. The state of every sign should be evaluated in points to be then summed up and at exceeding the sum of points being above "+20" one should predict unfavorable result. At the sum of "-13" prediction should be stated upon as favorable and at "-13" up to "+20" - prediction is considered to be doubtful.

EFFECT: higher accuracy of prediction.

2 ex, 3 tbl

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