Method of preparation of bioactive bone-plastic deprotex

 

(57) Abstract:

The invention relates to medicine, namely to the harvesting and preservation of bone tissue, and may be applied in the "bone banks" to ensure osteoplastic material health care institutions. To obtain material deproteinizing bone after washing with sterile distilled water crushed to particle size of 0.1-0.5 mm, obtained deproteinizing bone meal are combined at a temperature of 38-40C with 9% gelatin, prepared on preserving solution with the addition of antibiotic, preserved at a temperature of-20C and sterilized. Antibacterial drug, for example, reflin add in a dose corresponding optimal local antibacterial. Effect: method provides for obtaining bioactive bone-plastic deprotex plastic consistency with osteogenic properties, preventive antibacterial effect.1 C.p. f-crystals.

The invention relates to medicine, namely to the harvesting and preservation of bone tissue, and may be applied in the "bone banks" to ensure bone-plastic material institutions SDO means (Istrana L. P., Astranova E. C., Kurdyumov, S., and others, patent of Russian Federation №2091083), the main components of which are collagen, hydroxylapatite and the medicinal substance. The present invention is used hydroxylapatite ceramics obtained in the production process, similar in its properties with the mineral component of bone tissue.

The degradation process hydroxylapatite ceramics depends on its physico-chemical characteristics, which are largely determined by the conditions of synthesis of ceramics.

The process of biodegradation of ceramics also affect the structure of the porosity, i.e. the size of pores and their architectonics, play that during synthesis is not always possible.

The use of collagen as a liaison not always indifferent to the immune system as a possible manifestation of immune immunity on the part of the body.

The amount of medicinal substance (antiseptics) is up to 30% by weight of funds that exceeds the minimum required number several times and can hardly be justified.

A method of obtaining bone graft (E. A. Rama, such as roadside, Y. C., Atican Author light of what otheractivities biological origin, not always convenient, due to the stable form, the complexities of its modeling, and the possibility of the eruption of the graft through the mucous membrane of the mouth and the likelihood of incomplete filling of the bone defect. In addition, difficult to use deproteinizing bones with infected cysts of the jaws, as the treatment process becomes a multi. The period of formation of the regenerate long enough due to the lack of collagen supplements (for stimulation of osteogenesis).

The objective of the invention is obtaining bioactive bone-plastic material on the basis of components of biological origin, plastic consistence, having osteogenic properties, local antibacterial effect.

Achieving these objectives will help to increase the final results of the bone and plastic surgery, to optimize the processes of reparation and restore bone structure, damaged by the disease process, due to the properties inherent in the earnings of this material:

- high biological compatibility;

- lack of immune response;

- prevention of suppurative complications;

- stimulating ellenie and restoration of bone structure;

- complete replacement of the bone;

direct biochemical link carbonitesetuplite with the bone.

The positive effect of the proposed method for the preparation of biologically active bone-plastic material is achieved by the use of crushed deproteinizirovanny Xeno - or allobone.

As the link uses a 9% solution of gelatin (product of partial hydrolysis of collagen) in preservative fluid (11% sucrose solution, 0.9% NaCl, and others). It is important that the amino acid composition of gelatin refers to defective proteins and contains many residues of glycine, Proline, hydroxyproline, alanine (i.e., substances which take part in the building of new bone), which reduces the risk of immunological reactions. In addition, the biomaterial is injected antibiotic, for example, reflin (the product of the first order for surgical prophylaxis) in a ratio of 0.5 mg to 10 ml of 9% solution of collagen.

The problem is solved due to the fact that deproteinizing bone after washing with sterile distilled water crushed to particle size of 0.1-0.5 mm, obtained deproteinizing bone meal with the material of the drug, preserve at a temperature of-20C and sterilized. Antibacterial drug, for example, reflin add in a dose corresponding optimal local antibacterial.

The method is as follows.

Allogenic or xenogenic fragments of head and condyles of the hips (the spongy areas of the long bones) released from soft tissues and cut into fragments of size 321 see for 4 hours is subjected to mechanical cleaning by rinsing with cold running water, then perform the deproteinization by placing a piece of tissue in a 0.01% solution himopsina, and incubated at S within 72 hours. The material is then washed with cold running water for 40 minutes. Then the material is poured 10% hydrogen peroxide solution and placed in a thermostat at C for 48 hours, then washed with flowing water for 4 hours. The control is carried out on the median cut fragment under increase of 3 diopters.

No elements of the bone marrow shows the complete deproteinization bone. Then the material is placed in a liquid ether for 6 hours, changing the reagents every 2 hours at room temperature, dried in a sterile room under the fan for 15 minutes, sing 5 minutes Placed under the fan for 4 hours in a sterile box. Then use bone cutters are crushed to the size of 2-5 mm, further grinding in electric coffee to particle sizes of 0.1-0.5 mm

To prepare galatarasay environment in a heat resistant flask containing a particular preservative, for example 11% solution of sucrose, gelatin is made from the calculation to get a 9% solution. Food gelatin is better to take the first or higher grade.

The number konserviruyushchie solution is determined by the volume of the prepared material. Leave for 1-1 .5 hours at room temperature before swelling of gelatin.

The flask placed in a water bath and heated until complete melting of gelatin. During heating it is necessary to make periodic stirring of the contents of the flask by stirring.

Heladerias environment is cooled to +4C and mixed with an antibiotic, such as replynum, in a ratio of 0.5 mg to 10 ml of a 9% gel. The next stage in the ratio of 3:1 (10 g flour 20 ml of a 9% gel =3 g gel) combine flour from DPGC with heladeria environment and cooled to room temperature. Packed in 0.25 and 0.5, Preserved by freezing at-20C. Then sterilize gaseous oxide and fragments, rinse with water and deproteinization when S 0.01% solution himopsina within 72 h and 10% hydrogen peroxide solution for 48 h, the processing liquid ether, 10% solution of lithium chloride for 16 h and washing with sterile distilled water, characterized in that deproteinizing bone after washing with sterile distilled water crushed to particle size of 0.1-0.5 mm, obtained deproteinizing bone meal are combined at a temperature of 38-40C with 9% gelatin, prepared on preserving solution with the addition of antibiotic, preserve at a temperature of-20C and sterilized.

2. The method according to p. 1, characterized in that the antibacterial drug, for example, reflin add in a dose corresponding optimal local antibacterial.

 

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