Method of production of crop products and used in the composition for treatment of plants

 

(57) Abstract:

The invention relates to agriculture and can be used in the technology of crop production. Presowing seed treatment and/or treatment of vegetating plants provide a composition containing culture fluid of Bacillus nigricans and Streptomyces grisioviridis with the addition of Emistim and adhesive. The use of the invention will reduce the accumulation of toxins in the production of crop products. 2 S. and 2 C.p. f-crystals, 3 tables.

Group of inventions relates to the technology of crop production and composition of biofungicide.

The known method of production of crops capable of handling plants Bacterica, or Alienum In, or Aliminum With, or Gameiro, or Gliocladium, or Mikoshiba, or Cellobacterin, or Gastropacha and harvesting (Monastic O. A., Yaroshenko Century A. the Impact of biologics on the development and toxicobrain field strains of Fusarium frost // Reports of the Russian Academy of agricultural Sciences, No. 3, 2002, S. 22-23).

The disadvantage of this method is the accumulation of toxins in the target product.

Known tehnicheskim the result of a group of inventions is getting a new composition of biological fungicide, the use of which when in crop production can reduce the accumulation of toxins in the target product.

This result is achieved in that in the method of production of crop products, providing the plant treatment composition of the biological product and the harvest, according to the invention as a biological product, a mixture of the culture fluid of Bacillus nigricans with a titer of cells 109and the culture fluid of Streptomyces grisioviridis with a titer of cells 109taken in the ratio by volume of 3:1, with the addition of Emistim in the amount of about 0.05% by volume.

A preferred variant embodiment of this invention provides for processing a biological product at the pre-stage at a flow rate of the mixture cultural liquids of about 4 l/t of seed and/or at the stage of the growing season when the flow of the mixture of the culture liquids 4-5 l/ha planting.

The same result is achieved in that the composition for treatment of plants containing microorganisms and targeted supplements, according to the invention contains the microorganisms in the culture fluid of Bacillus nigricans with a titer of cells 109and the culture fluid of Streptomyces grisioviridis with a titer of cells 109taken in saliceto about 0.5% by volume of the mixture cultural liquids.

The composition is prepared by mixing the culture fluid of Bacillus nigricans and Streptomyces grisioviridis with a titer of cells 109. Upon receipt cultural liquids with different dilutions of cells to bring up given by removing part of the liquid phase through an ultra-filter or by adding sterilized water. The cultural mixing of liquids is carried out in a ratio by volume of 3:1. Next, the mixture was added to about 0.05% Emistim (List of pesticides and agrochemicals permitted for use on the territory of the Russian Federation in 2001. Supplement to the journal "Protection and quarantine of plants", No. 3, 2001) and about 0.5% adhesive by volume of the mixture cultural liquids. The accuracy of the compliance ratio of the volume of the culture fluid and dispensing of target components is determined mainly by the accuracy of the used metering equipment.

When crop production at the pre-stage and/or at the stage of the growing season the plants are treated with the obtained composition preferably at a flow rate of the mixture of the culture fluid 4 l/t of seed or 4-5 l/ha planting. Treatment leads to the destruction or inhibition of phytopathogenic microflora and not it stimulates the secretion of toxins. After harvesting the toxins in the target producao analogue.

To confirm the achievement of the technical result was a series of experiments. The composition was prepared using Bacillus nigricans 132, Streptomyces grisioviridis MO and SiO2gel as an adhesive. As the test cultures used wheat varieties Delta and Stefanka and barley varieties Skorokhod, because Zernovo culture in the most heavily affected toxinogenic microflora.

The composition in an amount of 4 l/t were processed grain at the pre-stage. Treatment effectiveness was assessed by comparison with untreated control lesions toxinogenic fungi and germination of grain. The results are summarized in table 1.

From table 1 it is evident that the proposed composition has a pronounced fungicidal activity when pre-sowing seed treatment and does not reduce their germination.

The same varieties of wheat and barley were treated at the stage of vegetation obtained composition at the rate of 4 or 5 l/ha planting. Used the planting of grain, of the past and not the past preplant. After the harvest was determined by the incidence of grain in comparison with the untreated control. For all series of experiments, the results fluctuated within the range of experimental error relative to srednegodnaia activity in the treatment of vegetating plants.

Collected grain was studied on the content of the most common toxins. Were used for comparison of the control grain obtained using raw grain with untreated plots, and the reference grain, obtained using known biological products Backoff and Alien With in accordance with the rules of their application. The results are summarized in table 3.

Thus, the proposed group of inventions allows you to get a new fungicidal composition of a biological product, the use of which when in crop production can reduce the accumulation of toxins in the target product.

1. Method of production of crop products, providing the plant treatment composition of the biological product and the harvest, characterized in that as a biological product, a mixture of the culture fluid of Bacillus nigricans with a titer of cells 109and the culture fluid of Streptomyces grisioviridis with a titer of cells 109taken in the ratio by volume of 3:1, with the addition of Emistim in the amount of about 0.05% by volume.

2. The method according to p. 1, wherein the treatment is carried out at the pre-stage when the flow is at perform on the stage of the growing season when the flow of the mixture of the culture liquids 4-5 l/ha planting.

4. The composition for treatment of plants containing the microorganisms and the target additives, characterized in that it contains the microorganisms in the culture fluid of Bacillus nigricans with a titer of cells 109and the culture fluid of Streptomyces grisioviridis with a titer of cells 109taken in the ratio by volume of 3:1, and the target additives Emistim in the amount of about 0.05% by volume and the adhesive in an amount of about 0.5% by volume of the mixture cultural liquids.

 

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