The method of obtaining edible biologically active prefabricated and edible biologically active material is obtained in this way

 

The invention relates to the food, agricultural, and biotechnological industries. The invention relates to the production of edible biologically active semi-finished products based on microorganisms. The method of obtaining edible biologically active semi-finished shredding wet edible carbohydrate vegetable substrate, including food or agricultural waste. Then the crushed mass is subjected spontaneous fermentation in aerobic conditions due to its own microflora of the used substrate. Then from fermented substrate isolated single colonies. From the colonies emit pure culture thermotolerant yeast or yeast-like fungi, which are selected from the group of genera: Pichia, Candida, Rhodotorula, Metchnikovia, growing at a temperature of 30-37C. And for a further solid state fermentation as producers of biomass selected culture with the size of the cells (1,5-4,0)(3,0-10,0) µm and sow carbohydrate nutrient medium with a neutral reaction, which is applied on solid media, while providing humidity 30-75%. Conduct solid-phase cultivation in aerobic at the th culture. Then the grown culture inactivate obtaining the target product. The invention allows to obtain universal edible biologically active material with prebiotic properties for different biological objects, for both human and animal. The resulting intermediate product also contributes to a comprehensive regulation of various functions in humans and animals. 2 C. and 10 C.p. f-crystals, 11 PL.

The invention relates to food, agricultural and biotechnological industries, and for the production of biologically active semi-finished products based on microorganisms with enhanced nutritional value and ability for the integrated management of a number of functions in the body of the biological object, person or animal.

On the background of imbalanced diet, in particular the shortage of food in essential amino acids and vitamins, and adverse environmental impacts of the food or feed containing natural biologically active additives (BAA), becomes a vital prerequisite to ensure the normal functioning of a person or animal.

Lately wide row gastrointestinal tract by introducing multiplied on a dairy substrates of different strains of bifidobacteria and lactobacilli (patent RU 2102898, class. And 23 With 9/127, 27.01.1998; patent RU 2130269, class. And 23 With 9/12, 20.05.1999; patent RU 2064269, class. And 23 With 9/12, 27.07.1996 and others).

But these additives have certain disadvantages. Although bifidobacteria and lactobacilli in dairy foods and pharmacy bacterial drugs have some positive effect in the lumen of the gastrointestinal tract, but they do not always have a strong ability to colonize the intestinal mucosa in a particular organism and often transit, not providing a stable normalization of microbiocenosis of the gastrointestinal tract.

As a dietary Supplement can also be used yeast as specially grown and is a byproduct of primary production. When industrial production of wine, spirits, beer, kvass and other products accumulating mass of yeast after fermentation is almost not used, and if used, it is very inefficient. In addition, the number generated in these processes, the yeast is quite small, that is generally specific for anaerobic cultivation of yeast.

The yeast is able to grow on cheap substrates have a high growth rate, can change the composition of the processed substrate in a given healthy lifestyles is th ecosystems, in aerobic conditions relatively quickly form a large number of non-toxic biomass. Using a variety of substances can be directed to increase in the cell content of amino acids, vitamins and minerals.

Yeast grow well on nutrient media, which are by-products in the processing of fresh fruit, berries and grapes, vegetables, technical and agricultural plant products and wild-growing raw materials. Yeast provide a higher biomass yield than bacteria because their cells are larger.

A method of obtaining medical-preventive additive, based on the use of the life of the yeast (Borisenko N. A. Development of technology of fermented products based on grain rye. Abstract. dis. Kida. technology. Sciences. - Moscow - 1996 - 25 C.). According to him, to edible vegetable raw materials add the yeast Saccharomyces cerevisiae (vini) VKPM Y-511 and cultivated at a temperature of 20-40With, and obtained after growing the yeast culture inactivate and subjected to drying. The method allows using the obtained culture to neutralize the toxins of pathogenic and conditionally pathogenic bacteria and activate its own bacterial fluorescence is see one strain of yeast. It is known that orientation on a single, albeit highly productive strain of microbe-producer always carries an element of risk of failure because of accidental loss or degeneration of this strain. A good safeguard against such phenomenon is the presence of many equivalent interchangeable highly productive microbial cultures, and even better the existence of a permanent source of such crops and methods of replenishing them.

A known method for the production of protein drugs through non-conventional raw materials and yeast for animals, providing for the allocation of yeast and yeast-like fungi - producers of animal waste and from complex microbial starter cultures used in traditional for a number of countries the processes of proteinsare plant materials, and the bioconversion of such unconventional substrates as livestock effluents, waste storage and processing of fruits and vegetables, cellulosic waste crop production by submerged and solid-phase cultivation of yeast in these wastes (E. Borisenko, the Development of integrated technologies for protein drugs on the basis of alternative raw materials and yeast. Abstract. dis. Prof. technology. Sciences. - Moscow - 1990 - 51 S.).

Otono of microbiocenosis of the gastrointestinal tract of humans and animals, do not deal with the issues of bioconversion traditional food and fodder yeast, and also not used a combined fermentation using-driven bacterial complex.

A method of obtaining biologically active food cake mix and food biologically active product obtained on its basis (RF patent No. 2181018 April 10, 2002). According to this method, the bioconversion of plant substrates is carried out using cultures of the yeast Saccharomyces cerevisiae allocated from microbial cenoses wine raw materials, selected yeast is grown on neutral nutrient substrates by the method of deep or solid-phase cultivation, and cultures are used as food cake mix directly or after additional fermentation using bacterial cultures with probiotic properties.

However, in this method for the bioconversion of plant raw materials used yeast-Saccharomyces cerevisiae producers produce only wine raw materials, it does not account for the possibility of using yeast and yeast-like fungi of other genera and obtain yeast cultures from other natural food or waste. In addition Toei their temperature optimum growth is about 30But it is difficult to keep constant, and the temperature in the2-4With, especially overheating, help stop the growth of this culture. In this way not provided by the combined fermentation, in which beneficial bacteria grown on yeast cultures of different genera. Moreover, this method does not claim to universality in the production of both food and feed.

The principal difference of the claimed invention is the versatility of the proposed method of production of edible biologically active prefabricated and universal purpose of the semi-finished product in relation to all biological objects, in particular for man and animal. These analogs, it is necessary to say that, despite some similarities of a number of essential features, none of them is implemented such universal use that is claimed in this invention, and therefore none of them can be selected as the nearest equivalent.

The present invention is to develop a versatile and simple, resource-efficient and affordable way to obtain herbal universal Syed, for both human and animal. Universal edible cake mix is designed to produce various functional foods or bioactive forages on the basis of microbial biomass with prebiotic properties, which would help to provide the daily needs of a person or animal in essential amino acids, vitamins, macro - and microelements and other biologically active substances.

This task is solved in that a method of obtaining edible biologically active semi-finished product includes: grinding wet edible carbohydrate vegetable substrate, including food or agricultural waste, then the crushed mass is subjected spontaneous fermentation in aerobic conditions due to its own microflora of the used substrate, then from fermented substrate isolated single colonies from pure culture thermotolerant yeast or yeast-like fungi, which are selected from the group of genera: Pichia, Candida, Rhodotorula, Metchnikovia, growing at a temperature of 30-37With, and for further solid state fermentation as producers of biomass selected cultural dimension to the Yu put on a solid carrier, while humidity 30-75%, and conducting solid-phase cultivation under aerobic conditions at a temperature of 30-37C for 12-48 hours prior to the accumulation of 2-6 billion cells/g wet culture and then cultivated culture inactivate obtaining the target product.

As a solid carrier of the nutrient medium may use a porous cellulose starchy food product advantages, crushed porous non-food product dignity or apply fabric, intensely absorbing water and approved for use in food industries, such as belting.

The resulting intermediate product can be used wet or dried form.

In another case, a complete method the resulting intermediate product is separated into fine and coarse fractions, of which fine, liberated from the solid media are used as biologically active food additives (BAA) or as a functional material for feed monogastric animals, and coarse fraction containing solid carrier, or as a biologically active food basics, or as a dietary component to the military from the solid fraction of culture, which is suspended in the liquid fraction, is subjected to drying to obtain yeast concentrate. At the same time as the liquid for flushing with solid-phase grown cultures use water or milk.

For two-stage cultivation grown on solid culture or suspended in the liquid fraction are sterilized and used as a nutrient medium in the process of solid state or submerged culture for bacterial cultures with probiotic properties.

Grown bacterial cultures with probiotic properties, use wet or dried form.

Universal edible biologically active material with the food or feed purpose produce on any of the options above.

The technical result of the claimed invention is to provide a universal method of producing edible biologically active semi-finished product and ensuring the universality of the biologically active material with prebiotic properties for any biological objects human, including for children, dietary and special food for persons of elderly and senile age, working in dangerous and the basis of such universal edible cake mix easily, affordable and easy to get a variety of biologically active food additives (BAA), biologically active food basics and functional foods in various industries, yeast concentrates for various purposes, biologically active components and feed digesting animals, functional feed for monogastric animals, etc.

Universal edible cake mix has a high content of microbial protein, close to the animal protein content of essential amino acids, high ability to neutralize the toxic metabolites of pathogenic and conditionally pathogenic bacteria, as well as the ability to regulate and normalize the microbiocenosis of the gastrointestinal tract of biological objects. Unlike probiotics - live microorganisms inactivated yeast and yeast-like fungi in the body of biological objects function as prebiotics - stimulants probiotics and eigenfunctions of the digestive tract. Prebiotics are a kind of "backing" for the preservation of viability and increase growth of beneficial bacteria. Thus, prebiotics affect the well being of the human body, normalizing and expanding the functions intestine various diseases and intoxications, diarrhea, gastritis, enteritis, colitis, dysbiosis, impaired liver function, metabolism, etc.,

Yeast and yeast-like fungi of the genera - Saccharomyces, Pichia, Candida, Rhodotorula, Metchnikovia, Tpichosporon obtained from spontaneous communities food such as fruits, berries, vegetables, products of horticulture, etc. and most likely to obtain from samples with signs of microbial infections. Edible raw materials used in the solid hydrated, semi-solid or liquid form. As an edible vegetable substrates using fruit, vegetable, grain raw materials (crushed or crushed grain, flour of different types of cereal grains and legumes, grain malt, bran, flour from grain straw crops), grass meal, substandard products and waste from the food and feed industry.

Yeast culture get on agar nutrient medium with the addition of antibacterial antibiotics, as standard, intended for the cultivation of yeast and nutrient media from the liquid fraction of wet carbohydrate food substrates dignity.

For example, carrot or beet agar obtained when the ratio of the vegetable pulp and water is 1:2 or 1:1 sootvetstvyet, as Apple juice, grape juice for biomass growth of these yeasts (table. 1). The selection of the most productive cultures of yeasts and yeast-like fungi are when growing them within 12-48 hours on solid substrates with pH close to neutral in aerobic conditions, while maintaining the temperature of 30-37C and a humidity of 40-60%. The criterion of efficiency growth of cultures used is the accumulation of cells in inkubiruemykh inseminated by these cultures materials which are much more intensively at thermotolerant yeast and yeast-like fungi from the group of genera: Pichia, Candida, Rhodotorula, Metchnikovia, with cell sizes of 1.5-4.03,0-10,0 µm (table. 1, 2).

When designing solid nutrient media along with carbohydrate substrates, rich reducing substances, as activator of the process of reducing consumption of these substances and more active accumulation of biomass of yeast used such loosening the components of these environments, such as cereal bran, and such non-starchy cellulose substrates as malt sprouts, straw flour, etc., (table.3, 4).

As a solid carrier carbohydrate substrate and growing on it draw the food industry, and starchy foods can be used as an independent substrates yeast bioconversion, and as components that contribute to salustiano other carbohydrate substrates and enhance their shvatyvaemost with cloth material (PL. 5).

When yeast bioconversion of plant product as the basis for regulating the humidity of the solid-phase culture, can be used himself vegetable product in the crushed and dried or dried solid waste processing in the previous production cycles (table. 6).

When designing nutrient media for solid-phase cultivation of yeast as forming the basis of the solid medium used delusinne, crushed, or flaked grain, as the humidifier solid Foundation is crushed into pulp vegetables, roots, berries or fruits, mainly with neutral pH, as additional humidity controller and stimulator of the growth of the yeast - hygroscopic porous solid edible products (table. 7).

Grown on solid nutrient medium yeast or yeast-like fungus inactivate and use wet or dried to be separated into a fine fraction, more rich yeast component, and a coarse fraction, more scarce yeast. The first fraction can be used as biologically active food supplements, and the second being the basis of this food (table. 8).

If drying subjected to washing with solid-phase culture water, milk or other liquid separated from the solid fraction of the culture, in this case can be obtained yeast concentrates, relatively much richer yeast biomass (table. 9).

Suspended in liquid yeast suspension or obtained from this concentrate is used as a semi functional food for man or feed for monogastric animals, and coarse cellulose fraction - as a component of feed for digesting animals.

When a two-phase culture grown on yeast or yeast culture or suspended in the liquid fraction adding additional nutrients or without them as secondary nutrient environment provided by cultivation of bacteria with probiotic properties - solid-phase or in-depth, under aerobic or anaerobic conditions depending on the type of target bacterially into fractions intended for use as a universal biologically active material for food or feed. The use of yeast culture as a nutrient medium for bacterial culture gives the semi-finished product at the same time pre - and probiotic properties.

Industrial cultivation of bacteria are on the sterilized solid-phase yeast culture or yeast suspension. While bringing moisture to the optimum in solid nutrient medium for production of bacteria, can be realized in such animal products as milk.

Seeded with bacteria materials incubated at a temperature 37,02,0With under aerobic or anaerobic conditions depending on the species grown bacterial culture. In table. 10 presents the results of the cultivation of freshly isolated cultures of bacteria of the genera Escherichia and Lactobacillus dried on solid-phase yeast cultures obtained on trubunal nutrient medium. Bring humidity up to 60% in solid-phase bacterial cultures were made of whole milk. Growing cultures of Escherichia on solid media were sterile Petri dishes and lactobacilli in sterile cylinders with lids height 30 see Grown active bacterial culture used in humid is nom.

When implementing this technology with onselectionchanged cultures of yeast or yeast-like fungi and bacteria are most active in the production of biomass or biologically active substances, such semi-finished product can become the basis of the food group or even mass application. In particular, a model of culture for universal edible biologically active or semi functional food mass application or biologically active feed can serve isolated from grape wine materials culture yeast-like fungus, which the Department of lower plants MSU (I. Y. Chernov) was identified as Pichia(Candida) guilliermondii yeast this culture is currently the most active producer of biomass and are encouraged to implement one - and two-phase bioconversion of plant raw materials.

The invention is demonstrated by the following examples, which, however, do not cover, but do not limit the claims of the invention.

Example 1. Method for the production of universal edible material for food and feed of grain and straw of cereal crops.

The straw is shredded standard the emergency by weight of water. After cooling to 30-35To make a yeast of the genus Candida and milled into flour grain to a moisture content of the mixture 55-60%. Planted material placed on Protvino or conveyor belt when the layer thickness of not more than 5-6 cm, incubated for 24 hours at a temperature of 30.0-37,0C and a relative humidity of about 100%. The resulting culture inactivate high temperature, dried to a moisture content of 8-10%, is subjected to rough grinding, on sieves with mesh sizes up to 1.0 mm is separated into a fine fraction-Trouveroy flour and chopped straw mixed with progrevanii flour. The first fraction is used as a functional material for food and feed monogastric animals, the second fraction is used as a biologically active component of feed for digesting animals.

Example 2. Method for the production of universal edible semi-finished product for production of food and feed from secondary raw materials for sugar production.

To hot raw beet sugar beet pulp add crushed into pulp fight beet, or crushed into pulp tails beet up to 30% by weight, or molasses to 10% by weight. The mixture is cooled to 30-35C and a relative humidity of about 100%. The resulting culture inactivate high temperature dried to 8-10% moisture and stored in a cool, dry place.

Example 3. A method of producing bread with high biological activity of the universal edible biologically active intermediate product.

As a growing substrate, a mixture of wholemeal wheat flour and straw flour in the ratio of 1:1. Mixture are steamed under stirring, cooled, make the culture yeast-like fungus Pichia(Candida) guilliermondii yeast and, if necessary, adjusted with sterile water to a moisture content of 55-60%. Planted material placed on Protvino or conveyor belt when the layer thickness of not more than 5-6 cm, incubated for 24 hours at a temperature in rastellini camera 30,0-37,0C and a relative humidity of about 100% to achieve 2-6 billion cells/g of the Obtained culture is suspended in water with a temperature of 65-75With the rate of achievement in suspension of 1.2-1.5 billion cells/ml suspension, stirred for 30 minutes and then divided into the de direct feed digastricus animals and the liquid fraction is used as a material for bread.

Example 4. A method of producing semi-finished product for biscuits with high biological activity of the universal edible biologically active semi - fermented flour.

From wheat flour 2 nd class with a moisture content of about 14% to prepare a 25% suspension in water. The suspension is brewed with stirring, cooled to 30To make a yeast of the genus Rhodotorula. Planted a paste applied to the strip of canvas belting and incubated for 24 hours at a temperature in rastellini camera 30-37C and a relative humidity of about 100%. After incubation pasty culture removed from the fabric and used as a semifinished product for biscuits.

Example 5. Method for the production of prophylactic feed for piglets from universal edible biologically active intermediate product.

Yeast culture of the genus Pichia or Candida of examples 1 or 2 are suspended in water with a temperature of 65-75With the rate of achievement in suspension of 1.2-1.5 billion cells/ml, stirred for 30 minutes and then separated on a sieve with a cell diameter of not more than 1 mm on the liquid and solid fractions. Terut with mixed fodder for pigs in a 1:1 ratio. Wet feed is fed immediately after cooking, or dried one of the possible ways. In this form it is suitable for storage and transport.

Example 6. Method of manufacturing progrevanii cereals.

Yeast of the genus Pichia according to example 2 are suspended in water with a temperature of 65-75With the rate of achievement in suspension of 1.2-1.5 billion cells/ml, stirred for 30 minutes and then separated on a sieve with a cell diameter of not more than 1 mm on the liquid and solid fractions. The solid fraction in the wet or dried form are sent to the feed digastricus animals, and the liquid fraction is mixed with grain pellets at a ratio of 1:1. Stand until the liquid is completely absorbed by the grains of cereals. Wet cereal or cooked until ready to eat steamed or dried to 8-10% moisture content one of the possible ways. In dried form pellets suitable for storage and transportation and can be used as a regular grits.

Example 7. A method of obtaining a functional caviar from fermented vegetables.

Crushed into pulp vegetables (zucchini, eggplant, tomatoes, etc.,) are steamed, cooled, make a yeast of the genus Metchnikovia. Required humidity octanal the lead analogously to example 1 and 2. Grown culture add concentrated tomatopaste, vegetable oil, salt, flour, onions, herbs, spices. The resulting product, if necessary, moisturize and razvarivat. Gomogenizirovannogo weight Packed and sterilized.

Example 8. A method of obtaining a soup refills from fermented vegetables.

Crushed into pulp vegetables - carrots, beets, tomatoes, etc. are steamed, cooled, make the culture yeast-like fungus Pichia(Candida) guilliermondii yeast. The desired humidity set with heat treated cereal bran. Incubation of cultures behave analogously to example 1,2. Grown culture inactivate, dried, crushed and Packed. This product is used as a functional soup refills.

Example 9. A method of obtaining a fermented cereals.

Pearl barley fried, soaked in 1:1 ratio boiled water for 12 hours, making culture yeast-like fungus Candida. The desired humidity set with heat treated wheat bran. Incubation of the cultures are similar to examples 1 and 2. Grown culture inactivate, dried, Toivanen and sifting divided into two factions - grain and otrubaniyu characteristics, precultural.

Fresh tomato pomace pasteurized, cooled, make a yeast of the genus Metchnikovia. The desired humidity set with heat treated dried tomato pomace. Incubation of cultures behave analogously to example 1, 2. The resulting culture is suspended in water with a temperature of 65-75With the rate of achievement in suspension of 1.0-1.2 billion cells/ml suspension, stirred for 30 minutes and then separated on a sieve with a diameter of 1.0 mm cells on the liquid and solid fractions. The solid fraction in the wet or dried form are sent to the feed digastricus animals, and to the liquid fraction is added to the concentrate of tomato juice to the norm of the dry substance content in the product. The resulting liquid is homogenized, Packed, sterilized and used as a protective drink for people.

Example 11. A method of obtaining a functional lactic acid product.

As a basis we take non-fat dry milk, mixing it with water in a ratio of 1:10. Milk is sterilized at 0.5 psi for 30 minutes. In sterile milk, chilled to 37.0With, make a complete leaven of bifidobacteria and lactobacilli in the amount of 5 and 0.5% dry powder concentrate �"https://img.russianpatents.com/chr/176.gif">C.

In the maturation process of the product after periodic mixing produce hourly metering of active and titratable acidity and the number of CFU of viable bifidobacteria. Control is the milk after you make the same starter without yeast concentrate. Dynamics of maturation of the product are presented in table.11.

In milk, which is made to ferment with yeast concentrate, the formation of a clot occurs within 6 hours, while the milk concentrate is not included, thickens after 8 hours.

For the final maturation of the sample is placed for 6 hours in the refrigerator when 4,0C. the Sample with the addition of yeast concentrate is a product with a pleasant sour taste and smell homogeneous thick consistency. During 7 days of storage the number of CFU of viable bifidobacteria is kept within lg 8.

The obtained results indicate that the stimulatory action of yeast concentrate on bifidobacteria in their cultivation in milk. When designing dairy products it allows to optimize the production process by reducing the time of cultivation, increased accumulation of organic acids, to obtain p the practical performance.

Thus, this invention allows to obtain a wide range of food products with bioactive properties on the basis of universal edible cake mix, such as a variety of bakery, pasta, cookies, crackers, dairy products, yoghurts, dried and cooked breakfasts, various discontented, vegetable semi-finished products, soup refills, vegetable purees of semi-liquid or dry for a child or gerodietetic power. At the same time, this method allows the same material to obtain valuable preventive animal feed.

High biological value, no toxic for human health and animal substances, good sensory properties, simple and affordable for mass production of the form of application and low cost in comparison with foreign analogues for the future use of this edible biologically active material and the unlimited possibilities of the production of food and fodder with regard to ecology, age and health of the biological object.

Claims

1. The method of obtaining edible biologically active on the m number of food or agricultural waste, then the crushed mass is subjected spontaneous fermentation in aerobic conditions due to its own microflora of the used substrate, then from fermented substrate isolated single colonies from pure culture thermotolerant yeast or yeast-like fungi, which are selected from the group of genera: Pichia, Candida, Rhodotorula, Metchnikovia, growing at a temperature of 30-37With, and for further solid state fermentation as producers of biomass selected culture with the size of the cells (1,5-4,0)(3,0-10,0) microns, which are then seeded with a carbohydrate nutrient medium with a neutral reaction, which is applied on solid media, while providing humidity 30-75%, and conducting solid-phase cultivation under aerobic conditions at a temperature of 30-37C for 12-48 h prior to the accumulation of 2-6 billion cells/g wet culture and then cultivated culture inactivate obtaining the target product.

2. The method according to p. 1, characterized in that the solid nutrient media environment using porous cellulose starchy food product dignity.

3. The method according to p. 1, characterized in that the solid is trichosis fact, as the solid carrier of the nutrient medium used fabric, intensely absorbing water and approved for use in food production.

5. The method according to any of paragraphs.1-4, characterized in that the obtained semi-finished products used in the wet form.

6. The method according to any of paragraphs.1-4, characterized in that the obtained semi-finished products used in dried form.

7. The method according to p. 5, characterized in that the resulting intermediate product is separated into fine and coarse fractions, of which fine, liberated from the solid media are used as biologically active food additives (BAA) or as a functional material for feed monogastric animals, and coarse fraction containing solid media as biologically active food basics or biologically active component of feed for digesting animals.

8. The method according to any of paragraphs.1-4, characterized in that the washing liquid with solid-phase grown culture, separated from the solid fraction of the culture, which is suspended in the liquid fraction, is subjected to drying to obtain yeast concentrate.

9. The method according to p. 8, characterized in that the yubom of PP.1-4, characterized in that the solid-phase grown culture or suspended in the liquid fraction are sterilized and used as a nutrient medium in the process of solid state or submerged culture for bacterial cultures with probiotic properties.

11. The method according to p. 10, characterized in that the grown bacterial cultures with probiotic properties, use wet or dried form.

12. Edible biologically active material characterized in that it is obtained by the method according to any one of paragraphs.1-11.

 

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