Spermicidal activity etoniya
Proposed: new spermicidal agent. The tool is atony (1,2-ethylene-bis-(N-dimethylcarbamate) ammonium dichloride), formerly known as antiseptic, antifungal (against Candida) and antimicrobial activity and is effective as a wound healing agent. The invention expands the Arsenal of tools for a specified purpose, the tool causes instant death of sperm cells at certain concentrations. table 1. The invention relates to medicine, in particular to the gynecologist.There are several substances that have spermicidal activity used in gynecological practice as a local contraceptive.1. Spermicidal activity has nonoxynol - 9-mono(personificaly) ether of polyethylene glycol is a potent surfactant, damaging the mucous membrane of the sperm, with antibacterial and antiviral activity [1, 3].The disadvantage of using this substance as a local contraceptive is expressed irritant effect on mucous membranes.2. Spermicidal activity set for gramicidin - cyclic (L-leucyl-D-dryer is of a given substance are expressed allergic reactions when applied topically, itching, contact dermatitis, loss of use during lactation .3. Spermicidal activity possesses chinosol-8-oksihinolina sulfate. Chinosol part suppositories “Contraception T” manufactured by the company Nizhpharm (Russia).The lack of chinosol is that the physical properties of chinosol is a powder lemon-yellow color with a very sharp persistent repulsive smell, and therefore stain underwear and hands, pervading smell of clothes, bedding and underwear.4. The closest activity is benzalconi chloride-alkylbenzyldimethylammonium chloride (prototype) with the local use of spermicidal action due to damage to the sperm membrane and pronounced antibacterial action [1, 3].Benzalconi chloride is the active substance of imported drug “farmatexa” issued in the form of vaginal tablets, suppositories, creams and tampons firm “InnoTek Internationale (France). The substance of benzalkonium chloride in Russia is not possible. With regard to domestic counterparts benzalkonium chloride, today they are missing.Disadvantages when it ispolzovaniya dermatitis, infection of the urinary tract, Candida, candidiasis, allergic vaginitis .The purpose of this invention is the identification of spermicidal activity etoniya - 1,2-Ethylene-bis-(N-dimethylcarbamate) ammonium dichloride.Atony currently used as antimicrobial, anti-inflammatory and stimulates reparative processes means for treatment of cracked nipples, vulvovaginitis, itching dermatoses, proctitis and sigmoiditis[3, 4, 5, 6]. As spermicidal funds to date atony was not used. New in this study is the possibility of using etoniya in gynecology as a local contraceptive domestic production due to the establishment of his spermatocide steps.The goal has been achieved by the establishment of the spermicidal activity etoniya.The study was conducted using cytomorphological methods on the normal sperm ejaculate person: counted the number of sperm motility, the number of actively motile sperms, time experiences of sperm was determined by the pH of the semen before and after equilibration sperm with aqueous solutions etoniya. All studies were conducted in vitro on 24 obrand>106the number of motile sperm 54,27±4,49%, the number of actively motile sperms 42,07±1,57%, pH sperm 7,546±0,18 time experiences sperm 315,38±14.24 from. After researching native sperm in each of the ejaculates was added to the studied aqueous solutions etoniya different concentrations: 0,01, 0,05, 0,1, 0,2, 0,3, 0,4, 0,5, 0,75, 1%. For control was used 1% solution of chinosol having spermicidal activity [1, 2, 3]. After mixing native sperm and solution chinosol in equal quantities and the subsequent deposition of the mixture on a glass slide the added in any form (mobile, motile and live) was not observed. The action of aqueous solutions etoniya was estimated at the time they are added to the native semen, 30 seconds, 60 seconds, 10 minutes, 15 minutes, 30 minutes and 1 hour after incubation. The volumetric ratio of sperm and investigated solutions was 1:1. The data are processed on the computer using the software package Statgraphics Plus. The results are shown in the table.Aqueous solutions etoniya in a concentration of 0.5% cause instant death of sperm, noting the complete absence of moving, actively motile and live spermatozoa, indicating spermicidal activity of aqueous solutions etoniya in Konz the Arat: monograph/ St. Petersburg: Special literature, 1996, S. 146-153.2. Kopteva L. N. Search contact spermicides/Obstetrics and gynecology, 1992. No. 2, S. 67-70.3. Register of medicines of Russia “radar - encyclopedia of medicine®”/Ed. 7th, Rev. and expanded. M: RLS, 2000, S. 130, 254, 659, 1118.4. Borima T. C., batuk because, Tomchuk Century, Treatment of cracked nipples achievei ointment//Obstetrics and gynecology. - 1970. No. 7, S. 73-74.5. He Kaskala A. N. The treatment of purulent wounds by atonium//Clinical surgery. - 1984. No. 1, S. 47-49.6. Klimenko A. N. The treatment of inflammatory diseases of the rectum and paraproctium drugs etoniya//Clinical surgery. - 1972. No. 10, S. 28-29.7. Clinical examination of the ejaculate/A. Y. Zubkov, M. E. Sitdikova, A. R. Patrina - Kazan: Kazan state medical University, 2001, 22 S.
ClaimsApplication etoniya-1,2-ethylene-bis-(N-dimethylcarbamate) ammonium dichloride as spermicidal funds.
FIELD: agriculture, animal husbandry, organic chemistry.
SUBSTANCE: antiseptic ointment comprises cationic surface-active substance, lower glycols, polyethylene glycols, water and ethylene glycol monophenolic ester and higher polyethylene glycols taken in the definite ratio of components. As cationic surface-active substance ointment comprises N-alkyl-N-alkoxycarbonylmethylhexahydroazipinium chloride or alkyldimethylbenzylammonium chloride, or cetylpyridinium bromide, or cetylpyridinium chloride, or 1,2-ethylenebis-(N-methylcarbdecyloxymethyl)ammonium dichloride, or ethylhexadecyldimethylammonium chloride, or chlorhexidine; as lower glycol ointment comprises 1,2-propylene glycol or polyethylene glycol-300, or polyethylene glycol-400; as higher polyethylene glycol ointment comprises polyethylene glycol-1500 or polyethylene glycol-3000, or polyethylene glycol-4000, or polyethylene glycol-6000. Ointment elicits the high antibacterial activity, broad spectrum of bactericidal effect and low irritating effect with respect to udder skin. Invention can be used for sanitary-hygienic treatment of udder of lactating cows for prophylaxis and rapid healing external damages of udder and nipples (arising cracks), prophylaxis of mastitis, enhancing milk purity by microbiological indices.
EFFECT: valuable antiseptic properties of ointment.
2 cl, 1 tbl, 22 ex