Antibacterial composition for the treatment of animals

 

(57) Abstract:

The invention relates to veterinary medicine, in particular to long-acting drugs for the treatment of animal diseases caused by bacteria and chlamydia. Antibacterial composition for the treatment of animals contains tylosin or its derivative, auxiliary substance - phthaloylglycine in the ratio of 5:1 respectively, and water in the following ratio, wt.%: tylosin (a derivative of tylosin) 5-10%; excipient 1-2%; water - the rest. The composition exhibits a high therapeutic effect in the treatment of respiratory and gastrointestinal diseases in animals and nitrobacteria cattle. 3 table.

The invention relates to veterinary medicine, in particular to long-acting drugs for the treatment of infectious animal diseases caused by bacteria and chlamydia.

The practice of treating diseases requires mainly the use of drugs that a single dose of which would preserve adequate therapeutic concentration of drug in the body for quite a long time, i.e., preparations with prolonged action that gives the best and txena of injury to animals.

To obtain prolonged drug use so-called auxiliary substances, which are used synthetic and natural high-molecular compounds (IUDs) and preference is given to natural Navy as the most biocompatible with the tissues of the body.

Known use as excipients in various pharmaceutical forms such as gelatin, a natural biopolymer. The latter is used for the manufacture of capsules, medicinal eye films, ointments, chewing and rassasyvanii tablets, i.e., for surface and oral use, for the purpose of delivery of the active substance (A. S. No. 2008922, a 61 K 47/00, 9/00, BI No. 5, 1994; A. S. No. 2069567, a 61 K 39/00, BI No. 33, 1996; Technology of medicinal forms, so 1, S. 288).

Traditionally for the treatment of respiratory diseases of animals used antibiotics such as erythromycin and oxytetracycline, chloramphenicol and tetracycline hydrochloride in diseases of the gastrointestinal tract.

However, the low solubility of these antibiotics limits their use in injectable form, which affects the duration of treatment. In addition, they are not intended to treat Necromastery is evani animals tylosin and its derivatives. Tylosin (thelan) is a macrolide antibiotic derived from fermentation of a strain of actinomycetes strain Streptomices fradiae produced in the form of salts tylosin (tylosin tartrate and tylosin phosphate), which are well soluble in water [Antipov C. A., Isaichev, P. Tylosin is an effective antibiotic. - Veterinary, 1981, No. 8, S. 63-65]. It inhibits the reproduction and growth of chlamydia, staphylococci, streptococci, pneumococci, clostridia, Erysipelothrix, mycoplasmas, dysentery pathogens of pigs, pascrell etc.

Tylosin is a low-toxic antibiotic and is considered to be highly safe drug. The risk of toxicity when using tylosin for animals is negligible.

However, tylosin is not effective enough against a group of bacteria belonging to the intestinal microflora, such as E. coli, salmonella, probably due to cellular impermeability. To him produces resistance of pathogenic microorganisms [Antipov Century A. Pharmacological properties of tylosin. - Veterinary medicine, 1989, No. 10, S. 60-61].

Moreover, due to the high solubility of the derivatives of tylosin they quickly dissolve from the introduction and excreted, therefore, to maintain a therapeutic concentration in the blood trail of the traditional forms of tylosin. - Veterinary, 1999, No. 7, S. 39].

Object of the present invention to provide pharmaceutical compositions of the prolonged action on the basis of tylosin and its derivatives having a high therapeutic effect in the treatment of respiratory and gastrointestinal diseases in animals and nitrobacteria cattle.

The problem is solved by developing and using an antibacterial composition for the treatment of animals on the basis of tylosin and its derivatives. And additionally, it contains the excipient - phthaloylglycine in the ratio of 5:1 respectively, and water in the following ratio, wt.%: a derivative of tylosin 5-10; excipient 1-2; water - the rest.

Offered as an excipient in the antibacterial composition phthaloylglycine is a yellow powder, soluble in water. It is non-toxic, stable during storage. Phthaloylglycine used in the production of films for the deposition of silver halides (Bibikova T. M., Carina B. B., Poplavskaya M. A. and others Influence phthaloylglycine on the persistence of photographic films. - Journal of scientific and applied photography and the No. 27 1991, patent RF №2026827, BI No. 2, 1995, MCI 02 F 3/02).

Used phthaloylglycine corresponds to THE 6-17-893-83.

The proposed composition is prepared by dissolving the components in water with stirring.

In table.1-3, presents data illustrating bactericidal activity (table.1) and therapeutic efficacy (table.2 and 3) proposed composition, hereinafter referred to as phthalan.

Determination of the bactericidal activity of the proposed medicinal preparation was verified by direct contact him in test tubes with the test microbes Staphylococcus aureus, Bacillus subtilis, Fusobacterium necrophorum.

Example 1. Bacterial suspension of Staphylococcus aureus with content 500000 microbial bodies were prepared in physiological solution. For tests used aqueous solutions of the composition with a concentration of 10.0; 5,0; 2,5; 1.25 mg/ml at a ratio of antibiotic:BB 5:1. From each sample (suspension of bacteria in saline solution + water solution compositions have been sowing in 10, 20, 30, 40 and 60 min of contact. For seeding used mastopathy agar. Crops were incubated at C during the day. The test had a threefold repetition.

In parallel were testing directly with Telesina.

Contagione the tests were carried out with other test objects Bacillus subtilis and Fusobacterium necrophorum at the respective concentrations of the composition.

In all cases, the bactericidal action of Talana was evaluated on the basis of lack of growth of the original bacteria cultures seeded on appropriate nutrient medium: Staphylococcus aureus, Bacillus subtilis - mastopathy agar, Fusobacterium necrophorum environment Kitt-Tarozzi. The results of the tests are presented in table.1.

Example 2. In the study of therapeutic efficacy of the composition for the treatment of gastrointestinal diseases in the subacute form for experimental purposes were used four groups of control calves 3-10 days of age in the amount of 9 animals each. In the first two groups of calves were injected intramuscularly in a dose of 5 ml/bird with an interval of 24 hours thelan and composition on the basis of the composition of example 1. In the treatment of 3 - and 4-th group of calves were used liometacen and tetracycline hydrochloride. When the trials used doses of the antibiotic according to the instructions on their application. The results of the study are given in table.2.

Example 3. In the study of therapeutic efficacy of the composition for the treatment of respiratory disease for experimental purposes were used four groups of control calves 1,5-2 months of age in the amount of six animals each. The first two groups of TSE 5 ml/goal. The third and fourth groups of calves were treated with the use of erythromycin and oxytetracycline according to the instruction on their application. The results of the study are given in table.3.

As can be seen from the tabular data, use of the compositions in the treatment of respiratory diseases reduces the duration of treatment of animals.

Example 4. In the study of prolonging effect of Talana experiment was performed using a 12 rabbits body weight of 2.5-3.0 kg

Animals of the first group (4 animals) introduced phthalan once intramuscularly in the waste before the dose used to treat calves 2-4 months of age with respiratory diseases in the amount of 3 ml/bird.

The second group of rabbits 4 goals were administered tylosin intramuscularly single dose of 3 ml/goal. The third group of rabbits served as control (antibiotic did not enter).

To determine the presence in the blood of experimental animals these preparations was carried out by sampling blood through 1, 3, 5, 10, 20, 30, 48, 60, 72 and 96 hours after their introduction.

Bactericidal properties of blood plasma experimental and control animals was performed, using as the test microbes 1,5-day cultures of the pathogen is

Experiments were 2-fold repetition.

Bactericidal effect in the blood plasma of animals of the first group were recorded within 1-72 hours, and 96 hours was observed a sharp decline in its bactericidal activity.

Bactericidal activity of blood plasma of rabbits of the second group were recorded within 1-10 hours, and 20 hours later observed a slight decrease in the initial concentration of the test bacteria.

In the study of blood plasma of rabbits of the control group in all cases demonstrated a slight decrease in the initial concentration of test objects.

As a bactericidal effect of Talana registered within 72 hours under the conditions of the experiment, and tylosin - 10 hours, you can come to the conclusion that the claimed composition (phthalan) has a prolonged action.

A slight decrease of the initial concentration of the test bacteria in the experiments with the blood plasma of control animals can be explained by the presence of bactericidal properties of blood of clinically healthy animals.

Thus, the proposed pharmaceutical composition on the basis of tylosin and its derivatives have a prolonged action, shows high therapeutically is Togo cattle.

Antibacterial composition for the treatment of animals on the basis of tylosin and its derivatives, characterized in that it further comprises an auxiliary substance - phthaloylglycine in the ratio of 5:1 respectively, and water in the following ratio, wt.%:

Tylosin (a derivative of tylosin) 5-10

Excipient - phthaloylglycine 1-2

Water The Rest

 

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FIELD: medicine.

SUBSTANCE: method involves carrying out hernia removal in intralaminar way. Posterior longitudinal ligament defect is covered with Tacho-Comb plate after having done disk cavity curettage. Subcutaneous fat fragment on feeding pedicle is brought to dorsal surface of radix and dural sac.

EFFECT: enhanced effectiveness of treatment; reduced risk of traumatic complications.

1 dwg

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