The method of preparation of tobacco for storage

 

(57) Abstract:

The invention relates to technology post-harvest processing and storage of tobacco. The method involves the use as immunostimulant for processing tobacco a drug derived from biomass micromycete Mortierella pusilla for a given technology, the subsequent exposure of tobacco, drying and processing of heated carbon dioxide containing single-base pair limit organic acid. The invention allows to simplify the technology by eliminating the need to use as immunostimulant pure substances.

The invention relates to technology post-harvest processing and storage of tobacco.

The known method of preparing tobacco for storage, providing for his handling of a mixture of arachidonic acid and chitosan, shutter speed not less than 3 h, drying and processing of heated carbon dioxide, containing a pair of monobasic organic ultimate acid (RU 2166887 C1, 20.05.2001).

The known method of preparing tobacco for storage, providing for his handling of a mixture of eicosapentaenoic acid and chitosan, shutter speed not less than 3 h, drying and processing of heated carbon dioxide containing pairs odnosloinosti because of the need to obtain the two components of the immune stimulator with a sufficient degree of purity.

The technical result of the invention is to simplify the technology.

This result is achieved in that in the method of preparing tobacco for storage, providing for his handling of a immunostimulant, an excerpt of not less than 3 h, drying and processing of heated carbon dioxide containing single-base pair limit organic acid according to the invention as immunostimulant drug use, obtained by sequential extraction of biomass micromycete Mortierella pusilla nonpolar solvent in the supercritical state, water, alkali, water, acid, water, alkali and water followed by combining the first extract from the solid residue, the number 1-1104mg/I.

The method is implemented as follows.

Dry biomass micromycete Mortierella pusilla extracted with a nonpolar solvent, for example carbon dioxide or hexane, supercritical condition. At this stage, separating the first extract used in the future when receiving the drug. Further biomass sequentially extracted with water, alkali, water, acid, water, alkali and water. Obtained after completing all these stages of extraction of the solid residue of the unsaturated fatty acids, basically presents arachidonic and eicosapentaenoic. The drug is treated with freshly harvested tobacco at the rate 1-1104mg/I. Since, as was established in the pilot test, the preparation does not include toxic substances and substances that can inhibit immunostimulirutuyu activity of chitosan, arachidonic and eicosapentaenoic acids, immune-stimulating effect of the drug was similar mixtures of chitosan with arachidonic or eicosapentaenoic acid. Empirically established that the treatment is effective when the consumption of the drug for at least 1 mg/ton, which is slightly higher than in the known technical solutions. This is due, probably, a significant amount of impurity substances that do not possess immunostimulatory activity. Further, the tobacco is dried and treated with heated carbon dioxide containing single-base pair limit organic acid, for example formic or propionic. As a result, after completion of all treatments is the destruction of the surface microflora of tobacco and the accumulation of substances, preventing the development of microflora after secondary contamination.

It should be noted that the upper limit of consumption plaguesome method. Tasting these products showed that the dosage of the drug is above the specified upper limit tobacco smoke acquires a shade of smell, which is not typical for tobacco products, and metal leaves the aftertaste.

Example 1.

Freshly harvested tobacco varieties dubek treated with mixtures of chitosan with arachidonic or eicosapentaenoic acid or mixture derived from biomass micromycete Mortierella pusilla using as non-polar extractant carbon dioxide as alkali caustic soda and hydrochloric acid, in quantities of 1 mg/t, incubated 3 h, dried, and treated with a stream heated to 30C carbon dioxide containing vapors of formic acid. Samples of tobacco after 1 month storage containerwith spores mold fungus Penicillium funiculosum in the number 21012spores/g and incubated at 36C within 7 days. The development of mold in the experimental and reference samples not detected. Tobacco smoke tobacco Smoking experienced and reference of the parties extraneous tones odorless, outside leaves no aftertaste.

Example 2.

Freshly harvested tobacco cultivar Samsun process of example 1, but using mixtures and drug derived from biomass micromycete Mogi propionic acid, in an amount of 10 g/t, and vapor propionic acid. In the same period of the sample of tobacco was inoculable in example 1, but using mold spores of the fungus Aspergillus niger, and carried out a similar test on funginertness. The result is the same as example 1.

Thus, the proposed method has simplified the technology due to the lack of necessary use as immunostimulant pure substances.

The method of preparation of tobacco for storage, providing for his handling of a immunostimulant, an excerpt of not less than 3 h, drying and processing of heated carbon dioxide containing single-base pair limit organic acid, characterized in that as immunostimulant drug use, obtained by sequential extraction of biomass micromycete Mortierella pusilla nonpolar solvent in the supercritical state, water, alkali, water, acid, water, alkali and water followed by combining the first extract from the solid residue, the number 1-1104mg/I.

 

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