The way to prevent the development of mold on tobacco

 

(57) Abstract:

The method relates to technology post-harvest processing and storage of tobacco. The way to prevent the development of mold on tobacco provides for the processing of the immune stimulator, an excerpt of not less than 3 h, drying and processing of heated nitrogen containing single-base pair limit organic acid. As immunostimulant drug use, obtained by sequential extraction of biomass micromycete Mortierella parvispora nonpolar solvent in the supercritical state, water, alkali, water, acid, water, alkali and water followed by combining the first extract from the solid residue, the number 1-1104mg/I. Method has simplified the technology due to the lack of necessary use as immunostimulant pure substances.

The invention relates to technology post-harvest processing and storage of tobacco.

Known way to prevent the development of mold on tobacco, providing for his handling of a mixture of arachidonic acid and chitosan, shutter speed not less than 3 hours, drying and processing of heated nitrogen containing pairs of monobasic organic ultimate acid (2169 RU is abotu a mixture of eicosapentaenoic acid and chitosan, the shutter speed is not less than 3 hours, drying and processing of heated nitrogen containing pairs of monobasic organic ultimate acid (RU 2166889 C1, 20.05.2001).

A common drawback of these methods is the difficulty of obtaining the two components of the immune stimulator with a sufficient degree of purity.

The technical result of the invention is to simplify the technology.

This result is achieved that the way to prevent the development of mold on tobacco, providing for his handling of a immunostimulant, an excerpt of not less than 3 h, drying and processing of heated nitrogen containing single-base pair limit organic acid according to the invention as immunostimulant drug use, obtained by sequential extraction of biomass micromycete Mortierella parvispora nonpolar solvent in the supercritical state, water, alkali, water, acid, water, alkali and water followed by combining the first extract from the solid residue, the number 1-1104mg/I.

The method is implemented as follows.

Dry biomass micromycete Mortierella parvispora extracted with a nonpolar solvent, for example carbon dioxide or Gex the treatment of the drug. Further biomass sequentially extracted with water, alkali, water, acid, water, alkali and water. Obtained after completing all these stages of extraction of the solid residue combined with the first extract. Thus obtained product contains mainly chitosan and higher polyunsaturated fatty acids, primarily arachidonic and eicosapentaenoic. The drug is treated with freshly harvested tobacco at the rate 1-1104mg/I. Since, as was established in the pilot test, the preparation does not include toxic substances and substances that can inhibit immunostimulirutuyu activity of chitosan, arachidonic and eicosapentaenoic acids, immune-stimulating effect of the drug was similar mixtures of chitosan with arachidonic or eicosapentaenoic acid. Empirically established that the treatment is effective when the consumption of the drug for at least 1 mg/ton, which is slightly higher than in the known technical solutions. This is due, probably, a significant amount of impurity substances that do not possess immunostimulatory activity. Further, the tobacco is dried and treated with heated nitrogen containing pairs of monobasic organic before the coming destruction of the surface microflora of tobacco and the accumulation of substances, preventing the development of microflora after secondary contamination.

It should be noted that the upper limit of the flow rate of the drug selected by the organoleptic properties of tobacco products derived from tobacco that is processed by the proposed method. Tasting these products showed that the dosage of the drug is above the specified upper limit tobacco smoke acquires a shade of smell, which is not typical for tobacco products, and metal leaves the aftertaste.

Example 1.

Freshly harvested tobacco varieties dubek treated with mixtures of chitosan with arachidonic or eicosapentaenoic acid or mixture derived from biomass micromycete Mortierella parvispora using as non-polar extractant carbon dioxide as alkali caustic soda and hydrochloric acid, in quantities of 1 mg/t, incubated 3 h, dried, and treated with a stream heated to 30C nitrogen containing vapors of formic acid. Samples of tobacco after 1 month storage containerwith spores mold fungus Penicillium funiculosum in the number 21012spores/g and incubated at 36C within 7 days. The development of mold in the experimental and reference samples not detected. Tobacco smoke tobacco Smoking experienced and reference of the parties is gerbrandy tobacco cultivar Samsun process of example 1, but using mixtures and drug derived from biomass micromycete Mortierella parvispora using as a nonpolar solvent hexane as alkali hydroxide, ammonia and propionic acid, in an amount of 10 g/t, and vapor propionic acid. In the same period of the sample tobacco inoculative in example 1, but using mold spores of the fungus Aspergillus niger, and carried out a similar test on funginertness. The result is the same as example 1.

Thus, the proposed method has simplified the technology due to the lack of necessary use as immunostimulant pure substances.

The way to prevent the development of mold on tobacco, providing for his handling of a immunostimulant, an excerpt of not less than 3 h, drying and processing of heated nitrogen containing single-base pair limit organic acid, characterized in that as immunostimulant drug use, obtained by sequential extraction of biomass micromycete Mortierella parvispora nonpolar solvent in the supercritical state, water, alkali, water, acid, water, alkali and water followed by Association of the first extract with solid East Jerusalem.

 

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