Method of cryopreservation succulent plant materials

 

(57) Abstract:

The invention relates to the food industry and can be used in the technology of canning succulent plant materials by freezing. Plant material before freezing is treated with a drug derived from biomass micromycete Saprolegnia parasitica for a given technology, and incubated for 2-5 hours. The use of the invention will increase the shelf life of succulent plant materials.

The invention relates to the technology of canning succulent plant materials freezing.

There is a method of cryopreservation succulent plant materials, providing its freezing and storage at the temperature of freezing (Trampolina T. C., Biochemical rationale for the technology of canning cold pepper vegetable (Capsicum L.) sweet varieties. (Abstract of Cand. K. so-called - Krasnodar: KPI, 1993).

The disadvantage of this method is the significant drop in the water-holding capacity of raw material, preventing its industrial processing, and small storage period.

The technical result of the invention is to reduce the fall of the water-holding capacity of raw materials and the expansion of the Oia succulent plant materials, providing its freezing and storage at the temperature of freezing, according to the invention, before freezing the raw material is treated with a preparation obtained by sequential extraction of biomass micromycete Saprolegnia parasitica nonpolar solvent in the supercritical state, water, alkali, water, acid, water, alkali and water followed by combining the first extract from the solid residue, the number 1-1104mg/t and incubated for 2-5 hours.

The method is implemented as follows.

Dry biomass micromycete Saprolegnia parasitica extracted with a nonpolar solvent, for example carbon dioxide or hexane, supercritical condition. At this stage, separating the first extract used in the future when receiving the drug. Further biomass sequentially extracted with water, alkali, water, acid, water, alkali and water. Obtained after completing all these stages of extraction of the solid residue combined with the first extract. In the composition of the drug are mainly chitosan and higher polyunsaturated fatty acids, including arachidonic and eicosapentaenoic, and are not toxic, carcinogenic, mutagenic and antipatriarchal in the above amount and incubated for 2-5 hours. Neither chitosan nor one of these acids do not interact with receptors of higher plants and are embedded in their membranes, and up to 90% of exogenously added acids already in the first hour is found in the lipid composition of plant material, mainly in the phospholipids, and the main part of the chitosan and the acid residue immediately assimilated. Because the combination of these acids with chitosan is a synergistic elicitors, as part of the preparation of the biomass micromycete Saprolegnia parasitica not contain substances that can inhibit this kind of activity, their assimilation leads to the formation at the cellular level signal immunization, which plant tissue to implement after 2-5 hours from the introduction of the mixture. As a result, ultrastructural reconstruction of plant tissues, aimed at increasing the strength of cell membranes and the accumulation of native cryoprotectants. The result is reduced krivomazova subsequent freezing of raw material. Held by exogenous phospholipids introduced acid and chitosan under the action of the natural enzymes of vegetable raw materials are gradually released and support the immune status of the raw materials is not less than 4 months, which prevents destructionist. Upon further storage for about 2 months, gradual release of raw materials from state immunization with the gradual increase of speed of falling water-holding capacity to a level typical for the same raw materials, frozen and stored under the same conditions without pre-treatment of exogenous elicitors. In the maximum period of storage of raw materials, processed by the proposed method increases of at least 4 months compared with the closest analogue.

To obtain the correct comparative data the proposed technique was tested on the same varieties of sweet peppers, which are described in the closest analogue: a Gift of Moldova, Waxy senyushkina, Kapitoshka, hybrid 2A-744 and the hybrid Cross 533 in technological and biological maturity. Experimental batches were processed product derived from biomass micromycete Saprolegnia parasitica using as non-polar extractant carbon dioxide as alkali caustic soda and hydrochloric acid, or hexane, hydroxide of ammonia and propionic acid, respectively, in quantities corresponding to the boundaries of the specified interval. The fruits of technological maturity stage on the C tested varieties of sweet peppers, processed by the proposed method, after freezing the water-holding capacity does not fall below 55%, while in the freezing without processing in the closest analogue of this result has been achieved only for varieties of the Gift of Moldova in the biological stage of maturity when freezing to minus 9C. During storage, samples were kept humectant at a stable level within 4 months, regardless of the stage of maturity and control only in the stage of biological maturity. If further storage is installed that pepper technological maturity stage of pilot batches, starting with the 5 months of storage, the water-holding capacity begins to fall with increasing speed, which excludes the possibility of its industrial processing, since 8 months. Pepper has a biological stage of maturity similar trend was observed with 8 months, but increase the speed of falling water-holding capacity was faster, which limits the duration of its processing 11 months of storage.

Qualitatively similar results were obtained by freezing and storage of tomato varieties Veneta and Gift, black currant varieties Belarusian sweet plum varieties Omy method allows to reduce the loss of water-holding capacity and increase the age limit of the material.

Method of cryopreservation succulent plant materials, providing its freezing and storage at the temperature of freezing, characterized in that before freezing raw materials are treated product obtained by sequential extraction of biomass micromycete Saprolegnia parasitica nonpolar solvent in the supercritical state, water, alkali, water, acid, water, alkali and water followed by combining the first extract from the solid residue, the number 1-1104mg/t and incubated for 2-5 hours

 

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