The way to diagnose stages of gastric ulcer and 12 duodenal ulcer
(57) Abstract:The invention relates to medicine, namely to a gastroenterologist, and relates to a method of diagnosis of the stage of gastric ulcer and 12 duodenal ulcer. The invention consists in that in the saliva of patients under investigation determines the amount of content peptides and when it is 0,882 units of optical density (TU) and higher judge about the ulcers; when it is within 0,881-0,539 TU - on the stage of incomplete remission; if the value is within 0,538-0,359 TU - on the stage of remission. Value content of peptides less 0,358 TU suggests that the patient is not suffering from a peptic ulcer. The advantage of the invention lies in the fact that the proposed method is informative, objective and does not require complicated invasive procedures. table 1. The invention relates to medicine, in particular to a gastroenterologist.Currently, the main method of diagnosis of gastric ulcer and 12 duodenal ulcer is a method of fibroesofagogastroduodenoscopia. However, this procedure is not always possible due to the hyperresponsiveness of the patient, impaired patency of privratnika (sub - and decomposion Anacostia and so on).There is a method of diagnosing stages of peptic ulcer disease (PUD) of stomach and duodenum 12 by determining epithelial index (EI) of the gastric mucosa, which is the ratio of the total number of epithelial cells to the number of the remaining cells (Patent 2098814).The main disadvantage of this method is a great complexity to it (you need to count all available cells per square millimeter slice of the gastric mucosa) and low specificity, which is determined by a large variety of cells, affecting the value of EI.A known method for the diagnosis of gastric ulcer and 12 duodenal ulcer by defining in the gastric juice of molecules of average weight (MSM). If the value of the content of MSM is in the range of 0.82-1.42 are units of optical density (TU) at a wavelength of 254 nm and 0.8 of 1.52 at a wavelength of 280 nm, it is judged on the presence of the ulcer of the mucous membrane and the exacerbation of the disease (Patent 2051388).The main disadvantages of this method are: the inability to use it to differentiate all stages of peptic ulcer disease (worsening, incomplete remission and in remission), and that it was not acceptable as a screening test, i.e., usnot invention is what in the saliva of patients under investigation determines the amount of content peptides and when it is 0,882 units of optical density (TU) and higher judge about the ulcers; when it is within 0,881-0,539 TU - on the stage of incomplete remission; if the value is within 0,538-0,359 TU - on the stage of remission. Value content of peptides less 0,358 TU suggests that the patient does not suffer from peptic ulcer disease.The method is as follows: the examined patients, pushing a sterile tube to the outlet duct of the salivary gland, receive a portion of the saliva. 5-8 ml her centrifuged for 15 minutes at 3000 revolutions per minute. The supernatant in a volume of 0.1 ml is poured into the centrifuge tube, was added to 4.9 ml of 96% ethanol, then centrifuged for 20 minutes at 3000 revolutions per minute. Then the supernatant examined for the spectrophotometer against 96% ethanol at a wavelength of 220 nm. This wavelength is determined by the content of peptides in biological fluids. The results are expressed in units of optical density (TU).Exacerbation of peptic ulcer disease is diagnosed when the value of the content of the peptides in saliva of patients 0,882 TU and above; if it is within 0,881-0,539 TU diagnosed with the peptides less 0,358 TU judge about the patient does not suffer from peptic ulcer disease.Example 1. Patient D., aged 26. He complained of recurrent pain in the epigastric region, appearing through 1.0 to 1.5 hours after a meal, nausea, vomiting, heartburn. Patients consider themselves to be within 4 days. Objective examination revealed tenderness in the epigastric region. General blood and urine analysis, biochemical analysis of blood within normal limits. The content of peptides in saliva of the patient was 1,214 TU. On the basis that the value of the content of the peptides in saliva was higher 0,882 TU, the patient was diagnosed with ulcers. At endoscopy in the bulb 12 duodenal ulcer detected ulcer size 0,40,30,3 see the Patient assigned to the appropriate treatment.Example 2. Patient P. 45 years. Within 1 month were outpatients regarding exacerbation of peptic ulcer of the stomach, which affects about 4 years. At the time of the survey no complaints. The content of peptides in saliva was 0,671 TU. On the basis of the fact that the value of the content of the peptides in saliva was within 0,881-0,539 TU was diagnosed with stage incomplete remission. Endoscopically detected ulcers “corner” of the stomach in a study of the venous disease 12 duodenal ulcer within 6 years. Complains of an unstable chair (alternating diarrhea and constipation), spastic pain during defecation. General blood and urine analysis, biochemical analysis of blood and coprological examination is within normal limits. The content of peptides in saliva was MX 0.317 TU. On the basis of the fact that the value of the content of the peptides in saliva were lower 0.358 TU was diagnosed with persistent clinical and morphological remission. Endoscopic examination revealed moderate deformation of the bulb 12 duodenal ulcer. Patient recommended further examination of the gastrointestinal tract, after which he was expelled organic pathology and diagnosed with irritable colon.Only offered by way of the surveyed 117 people. 41 during acute PUD 31 in the stage of incomplete remission, 27 - stage clinical-morphological remission and 18 healthy individuals.Normal value content of peptides in saliva was 0,291±0,021 TU.The obtained data are presented in below table.The proposed method is quite informative and does not require complicated invasive procedures. It eliminates the impact on the diagnosis of peptic ulcer subjective Issei. You can use it as a screening test in the examination of a large cohort of patients, including during routine examination. Weight it can be used for differential diagnosis, selection of the means and duration of treatment, and to assess the effectiveness of therapy.The way to diagnose stages of gastric ulcer and 12 duodenal ulcer, characterized in that in the saliva of patients under investigation determines the amount of content peptides and when it is 0,882 units of optical density (TU) and higher judge about the ulcers; when it is within 0,881-0,539 TU - on the stage of incomplete remission; if the value is within 0,538-0,359 TU - on the stage of remission, the value of the content of peptides less 0,358 TU suggests that the patient is not suffering from a peptic ulcer.
FIELD: medicine, psychiatry.
SUBSTANCE: one should isolate DNA out of lymphocytes of peripheral venous blood, then due to the method of polymerase chain reaction of DNA synthesis one should amplify the fragments of hSERT locus of serotonin carrier gene and at detecting genotype 12/10 one should predict the risk for the development of hallucino-delirious forms of psychoses of cerebro-atherosclerotic genesis.
EFFECT: more objective prediction of disease development.
FIELD: medicine, urology.
SUBSTANCE: one should conduct subcutaneous prevocational tuberculin test and, additionally, both before the test and 48 h later it is necessary to perform the mapping of prostatic vessels and at decreased values of hemodynamics one should diagnose tuberculosis. The information obtained should be documented due to printing dopplerograms.
EFFECT: more reliable and objective information.
1 ex, 1 tbl
FIELD: molecular biology.
SUBSTANCE: the suggested innovation deals with the fact that nucleic acids should be isolated directly out of the sample without pipetting stage but with the help of interconnected reservoirs being prepared beforehand. The above-mentioned vessels should be applied either separately or being interconnected according to standard microtitrating format. The sample should be mixed with a lyzing buffer and nucleic acids are bound with matrix in closed system including, at least, two interconnected reservoirs. Forced movement of sample's mixture and buffer back and forth from one reservoir into another one for several times through narrow passage provides their thorough intermixing. The method provides quick and safe isolation of nucleic acids.
EFFECT: higher efficiency.
44 cl, 4 dwg, 1 ex
FIELD: medicine, phthisiology, microbiology.
SUBSTANCE: diagnostic material is poured preliminary with chlorohexidine bigluconium solution, homogenized, kept at room temperature for 10-12 h and centrifuged. Precipitate is poured with Shkolnikova's liquid medium, incubated at 37oC for 3 days, supernatant part of Shkolnokova's medium is removed, fresh Shkolnikova's medium is added, and precipitate is stirred and inoculated on the dense cellular egg media. Sensitivity of the strain is determined in 3 weeks by the presence of growth in the control tube only. Invention provides enhancing precision and reducing time for assay. Invention can be used in assay for medicinal sensitivity of tuberculosis mycobacterium.
EFFECT: improved assay method.
FIELD: medicine, biotechnology, pharmacy.
SUBSTANCE: invention relates to agents used for treatment of pathological states associated with disorder of synthesis of neuromediating substances. Method involves the development of pharmaceutical composition and a method for it preparing. Pharmaceutical composition represents subcellular synaptosomal fractions: synaptic membranes, "light" synaptosomes and "heavy" synaptosomes prepared from gray matter of cerebral hemispheres from experimental animals based on the goal-seeking modification of humoral mediators of nerve endings transformed to synaptosomes in development and regression of malignant processes. The composition provides inhibiting the growth of tumor cells, to elevate span-life of patients with ascite Ehrlich's sarcoma, breast adenocarcinoma Ca-755, Wolker's carcinosarcoma-256.
EFFECT: valuable medicinal and anti-tumor properties of composition.
12 cl, 3 tbl, 3 ex
SUBSTANCE: method involves carrying out microscopic examination of blood serum samples taken from femoral vein and cubital vein. Femoral vein sample is taken on injured side. The examination is carried out before and after treatment. The blood serum samples are placed on fat-free glass slide in the amount of 0.01-0.02 ml as drops, dried at 18-30°C for 18-24 h. The set of pathological symptoms becoming larger or not changed after the treatment in comparison to sample taken before treatment, and morphological picture of samples under comparison taken from the cubital vein showing no changes or being changed to worse, the treatment is considered to be effective.
EFFECT: enabled medicamentous treatment evaluation in course of treatment to allow the treatment mode to be changed in due time; avoided surgical intervention (amputation); retained active life-style of aged patients.
FIELD: medicine, clinical toxicology.
SUBSTANCE: at patient's hospitalization one should gather the data of clinical and laboratory values: on the type of chemical substance, patient's age, data of clinical survey and laboratory values: body temperature, the presence or absence of dysphonia, oliguria being below 30 ml/h, hemoglobinuria, erythrocytic hemolysis, exotoxic shock, glucose level in blood, fibrinogen and creatinine concentration in blood serum, general bilirubin, prothrombin index (PTI), Ph-plasma, the state of blood clotting system. The state of every sign should be evaluated in points to be then summed up and at exceeding the sum of points being above "+20" one should predict unfavorable result. At the sum of "-13" prediction should be stated upon as favorable and at "-13" up to "+20" - prediction is considered to be doubtful.
EFFECT: higher accuracy of prediction.
2 ex, 3 tbl
FIELD: medicine, juvenile clinical nephrology.
SUBSTANCE: disease duration in case of obstructive pyelonephritis should be detected by two ways: either by detecting the value of NADPH-diaphorase activity, as the marker of nitroxide synthase activity in different renal department and comparing it to established norm, or by detecting clinico-laboratory values, such as: hemoglobin, leukocytes, eosinophils, urea, beta-lipoproteides, lymphocytes, neutrophils, the level of glomerular filtration, that of canalicular reabsorption, urinary specific weight, daily excretion of oxalates, arterial pressure, and estimating their deviation against average statistical values by taking into account a child's age.
EFFECT: higher efficiency of detection.
7 dwg, 1 ex, 6 tbl
FIELD: medicine, urology.
SUBSTANCE: the present innovation deals with differential diagnostics of prostatic cancer and other prostatic diseases at the stage of primary inspection. The method includes the detection of PCA and calculation of probability coefficient for prostatic cancer (PCC) by the following formula: where e - the foundation of natural logarithm (e=2.718…), PCA - the level of total blood PCA in ng/ml, V - patient's age in years. At PCC value being above 0.2 one should diagnose prostatic cancer and to establish final diagnosis one should perform polyfocal prostatic biopsy. The method enables to increase accuracy of diagnostics at decreased number of unjustified prostatic biopsies.
EFFECT: higher efficiency of diagnostics.
FIELD: medicine, biology.
SUBSTANCE: invention relates to nutrient medium used for accumulation of cells for the following cytological and/or immunocytochemical analysis carrying out. Invention relates to medium containing salts NaCl, KCl, anhydrous CaCl2, MgSO4 x 6 H2O, MgCl2 x 6 H2O, Na2HPO4 x 2 H2O, KHPO4, NaHCO3, and also glucose and Henx's solution, 10% albumin solution and polyglucin taken in the ratio 1:1:1. Invention provides enhancing the preservation of cells.
EFFECT: improved an valuable properties of nutrient medium.