The method of determination of crude ash in biological materials

 

(57) Abstract:

The invention relates to agriculture, specifically, to methods for determination of crude ash in biological materials (plant materials such as hay, haylage, silage, etc.,) and can be used in research and in the analysis of materials. In the proposed method in a weighed crucible make first right hand bat or legkorazdrazhimoy inorganic salts, for example, respectively chloride or ammonium nitrate (0.2-0.3 g), then a portion of the analyzed material (1.0-1.5 g). Heated in a muffle furnace for 1 h to a temperature of 200C and calcined 2-2,5 hours at a temperature of 525-C, cool, weigh, and calculate the result. The method allows to reduce the duration of the determination of crude ash (ash) by reducing the duration of annealing with 5-6 up to 2.0-2.5 hours.

The invention relates to agriculture, specifically, to methods for determination of crude ash in biological materials (plant materials such as hay, haylage, silage, etc), and can be used in research and in the analysis of materials.

Determination of crude ash, in particular in the feed, it is important to assess their quality and nutritional value.

Determination of crude ash wasp mineral (macro - and micronutrients) remain in the form of ash. The residue obtained by burning the sample analyzing material, consider crude ash.

The described method of determination of crude ash in feed by taking a sample of air-dried material in a pre-calcined porcelain crucible, heating it in a muffle furnace with a gradual rise in temperature to 200C. Slow start ashing necessary to avoid the scattering of small particles of the sample. After 50-60 min temperature in the muffle raise to 525-560C and burn charred mass up until the ash will not get a light gray color. For complete ashing samples at 525-560C takes 5-6 hours [1].

The disadvantage of this method is the length of the determination of crude ash (ash).

The purpose of the invention is the reduction in the duration of digestion. This goal is achieved by the fact that at first calcined and weighed crucible contribute a portion of finely divided inorganic bat or legkorazdrazhimoy in the conditions of the ashing salt, for example, respectively chloride or ammonium sulfate (0.2-0.3 g), then a portion of finely ground air-dried analyzed material (1.0-1.5 g). Then as usual, conducted heat content of the crucible in a muffle furnace at first to a temperature of 200C, and then calcined at the same time the residue from combustion are cooled and weighed. The difference calculate the content of crude ash. The addition of inorganic salt, which decomposes during combustion gaseous products or evaporates, promotes the drying of the material, increasing the surface contact of the material with oxygen and faster combustion.

The content of crude ash in fiber are calculated according to the formula (X %):

where a is the mass of the crucible with the raw ash, g;

in - mass of empty crucible, g;

n - linkage, g

The proposed method allows to reduce the duration of the ashing material from 5-6 hours to 2-2,5 hours

Example 1

In calcined crucible contribute finely ground 0,212 g of ammonium chloride and 1.0 g of air-dry material - grass hay. The crucible is placed in a muffle furnace and heated for 1 h to a temperature of 200C, then increase the temperature to C and calcined for 2 hours to obtain the gray mass. The crucible is cooled in a desiccator and weighed. The content of crude ash is 7.4%.

Example 2.

In calcined crucible as in example 1 make a finely divided 0.25 g of ammonium nitrate and 1.5 g of the analyzed material - silage pea with barley. The crucible is placed in a muffle furnace and nagrevaobespechivaet. The content of crude ash is 6.8%.

In the analysis of corn silage as in example 2, the ash content is 8.0%. Thus, the proposed method allows to reduce the duration of the ashing material more than 2 times.

Sources of information

1. Instructions for laboratory State agrochemical service for feed analysis. - M., 1978, S. 17 (appr. the head of the Main Department of chemicalization of agriculture Ministry of agriculture of the USSR A. Artyusinan November 25, 1977).

The method of determination of crude ash in biological materials by annealing the sample in the crucible at the beginning to 200C, then at 525-C, cooling, weighing and calculation result, wherein the first, contribute a portion of volatile inorganic or legkorazdrazhimoy inorganic salt, for example chloride or ammonium nitrate (0.2-0.3 g), then a portion of the analyzed material (1.0-1.5 g).

 

Same patents:

The invention relates to medicine, namely to biochemistry and immunology

The invention relates to medicine, in particular to oral and maxillofacial surgery

The invention relates to medicine, namely to methods for the rapid diagnosis of diseases of the thyroid gland instrumental methods

The invention relates to the analysis of analytes in a sample, particularly a biological sample through the binding of receptors and ligands, using the magnetizing fixtures to create a magnetic field in place of the sample and the detector device for measuring the magnetic properties of the sample

The invention relates to medicine, in particular, to reflexology, and can be used in the diagnosis, treatment and monitoring of patient health status of their biologically active points
The invention relates to medicine, in particular to Oncology
The invention relates to medicine, in particular to neurosurgery, neurology and traumatology

The invention relates to medicine, namely to addiction, and can be used for the diagnosis of chronic alcohol intoxication
The invention relates to medicine

The invention relates to biology and experimental medicine

FIELD: medicine.

SUBSTANCE: method involves examining blood erythrocytes. Blood erythrocyte suspension fluorescence intensity is measured at wavelength of 500 nm polarized along light axis and then at wavelength of 493 nm in perpendicular to the light axis for each polarization direction with following anisotropy value being calculated. Its deviation from norm proves availability of hemolytic disease.

EFFECT: accelerated examination process; reduced risk of traumatic injuries of fetus or newborn.

FIELD: veterinary medicine.

SUBSTANCE: method involves carrying out blood erythrocyte micro electrophoresis in alternating electric field with sign change frequency equal to 0.3-1 Hz. Electric field having current intensity equal to 4.5-5.0 mA is applied. Treatment is applied at 36-38°C. Oscillation amplitude being equal to 3 mm and less, endotoxicosis is detected.

EFFECT: high accuracy and simplicity of the method.

FIELD: medicine.

SUBSTANCE: method involves applying integrated diagnostics approach with blood serum examination being applied using infrared spectrometry methods. Sample absorption spectra in the area of 1200-1000 cm-1 are recorded, absorption peak heights having maximum at 1170, 1165, 1140, 1125, 110, 1070, 1025 cm-1 points are measured. Then, mean peak height C and ratio X of peak height at 1125 cm-1 point of maximum to C are calculated. X≥0.7 being the case, light myocardium injury degree is to be diagnosed. 0.7>X≥0.53 being the case, moderate severity degree case is to be diagnosed. X<0.53 indicates grave injury to take place.

EFFECT: high reliability of diagnosis.

FIELD: analytical methods in medicine.

SUBSTANCE: cells of tested organs are subjected to alternating cytological electrophoresis. When cell vibration amplitude rises by at least 20%, drug is regarded to be appropriate for treatment.

EFFECT: increased drug selection efficiency.

2 ex

FIELD: medicine, hepatology.

SUBSTANCE: the present innovation deals with detecting bilirubin content both before and after curative starvation, moreover, curative starvation one should introduce antipyrine at the dosage of 1 mg/kg, in a day it is necessary to detect antipyrine content in saliva and urine and bilirubin content and at bilirubin content being 30-40 mM/l one should starve at daily intake of 400-450 kcal/d for 3 d, then one should again detect bilirubin level and at its increase by 50-100% one should again introduce antipyrine at the dosage of 1 mg/kg and according to its delayed half-life by 20-50% it is possible to diagnose Gilbert's disease.

EFFECT: higher accuracy of diagnostics.

3 ex

FIELD: medicine, otorhinolaryngology.

SUBSTANCE: the present innovation deals with treating diseases of the upper respiratory tract. One should detect disorders of mucociliary transport (MCT) due to measuring the motor parameters of ciliated epithelium cilia, at least, the rate of mucociliary transport that includes material sampling for further testing as ciliary epithelial cells and registration of cilia fluctuations in human respiratory tract due to TV microscopy performed in patient's lifetime. Material for testing should be sampled from mucosal surface of respiratory tract, and therapeutic tactics should be chosen depending upon motor velocity of cilium's tip obtained based upon mathematical modeling followed by calculations by the formula: , where Vc - velocity of cilium's tip [mcm*sec1]; C1, C2, α - calculated as average values being characteristic for every of human body states under investigation: healthy persons, patients with either acute or chronic forms of sinusitis by the developed model of cilia movement; C1 - curvature of cilium's working length, constant being equal to 1/38 mcm-1; C2 - cilium's curvature from its working length up to the tip [mcm-1]; α - cilium's inclination angle against vertical position [degrees]; π = 3.1417 - constant; L, Ts and Te - parameters measured individually in every patient; L - cilium's length [mcm]; Ts - time from the onset of movement till maximal straightening of cilium's tip [sec]; Te - time of efficient impact [sec]. Moreover, the value being Vc = 9.6 - 11.3 mm/min corresponds to normal MCT functioning. For patients with chronic purulent sinusitis beyond exacerbation accompanied with MCT disorder it is necessary to prescribe complex therapy: surgical or conservative therapy with preparations of secretolytic and secretomotory action. For patients with chronic purulent sinusitis beyond exacerbation without any MCT disorder one should prescribe surgical therapy. All patients with acute sinusitis with MCT disorder should be prescribed complex conservative therapy with preparations of secretolytic and secretomotory action. The method enables to specify optimal therapy based upon scientifically proved indications of investigations by taking into account individual peculiarities of every patient.

EFFECT: higher efficiency.

5 dwg, 3 ex, 2 tbl

FIELD: medical engineering.

SUBSTANCE: device has non-absorbing substrate having hydrophilic target region, which is covered with reagent by applying non-impact micro-drop printing method to produce practically uniform reagent layer. The device is in particular usable for measuring blood coagulation time. Preferential invention embodiment involves determining blood coagulation time by carrying out monitoring of light transition through the target region as the blood sample cover interacts with the reagent.

EFFECT: high reliability of analysis results.

20 cl, 9 dwg

FIELD: medicine.

SUBSTANCE: one should register the values of pH and Redox potential (Red) of liquid means in bio-objects simultaneously. It is necessary to plot a graph ▵pH/▵Red according to fluctuations of variation values in measured parameters at their temporal diagram to transfer it into a columnar one where the height of every column is proportional to the area of this graph between neighboring measurements, correspondingly. According to the ratio of area sums of positive columns to that of negative ones during a certain period of time ( from a minute- to a year-long ones) one should conclude upon a state in a bio-object. The value ranged 0.5-2 is considered to be a standard. The method enables to provide efficient control for a two-phase systemic process.

EFFECT: higher efficiency and accuracy of diagnostics.

1 cl, 9 dwg, 5 ex, 1 tbl

FIELD: medicine, clinical neurology, neurosurgery.

SUBSTANCE: one should perform crystallographic study if cerebrospinal fluid and at protein concentration in liquor samples being from 0.50 g/l and higher at predominance of crystals as suppressed dendrites upon a crystallographic picture one should diagnose a malignant cerebral tumor, and at protein concentration being below 0.50 g/l and predominance of crystals as branched dendrites - a benign cerebral tumor. The innovation enables to fulfill not only due diagnostics of cerebral neoplasms, but detect the degree of tumor process malignancy at the early stages of its development.

EFFECT: higher accuracy of differential diagnostics.

3 dwg, 2 ex, 1 tbl

FIELD: biology, medicine, in particular histological investigation of shells with natural surface by using light-optical microscope.

SUBSTANCE: claimed method includes surface relief investigation by using plane field microscope in reflected light at one-side falling shadow-forming lighting. Light-reflecting ability of specimen surface is provided by silver impregnation. Said impregnation is carried out by specimen hold in 10 % silver nitrogen solution for 10 min followed by reducing thereof with 1 % ascorbic acid solution for 1 min.

EFFECT: method for investigation of natural shell surface relief of improved quality due to visual representation of surface three-dimension image on tissue level.

3 cl, 4 dwg

Up!