Antibodies with reduced total positive charge

 

The invention relates to medicine and relates to antibodies with reduced total positive charge. The invention includes a composition comprising a monoclonal antibody conjugated with Biotin, at least one of the many free amino groups present in the antibody, obtaining a modified antibody having smaller compared with the intact antibody total positive charge, increased antigennegative specificity, the invention also concerns a method for obtaining this composition, and a method of localization of the antigen in the mammal. The advantage of the invention is to improve the specificity of antibodies and improved pharmacokinetic characteristics. 3 S. and 13 C.p. f-crystals, 3 tab., 19 Il.

Description text in facsimile form (see graphic part).

Claims

1. A composition comprising a monoclonal antibody conjugated with Biotin at least one of the many free amino groups present in the antibody, obtaining a modified antibody having smaller compared with the intact antibody total positive charge, increased antigennegative juicy between velocities clearance F(ab)2fragments and intact antibodies of the same type, and chemical residue that is attached to the modified antibody.

2. The composition according to p. 1, characterized in that the chemical balance is the label.

3. The composition according to p. 2, characterized in that the label is a radioactive isotope.

4. The composition according to p. 3, wherein the modified antibody is attached to a radioactive isotope of a halogen or technetium.

5. The composition according to p. 4, characterized in that the radioactive isotope selected from the group consisting of125I and131I.

6. The composition according to p. 2, characterized in that the specified mark is recorded by a magnetic resonance tomography.

7. The composition according to p. 1, characterized in that the chemical balance is a biologically active molecule.

8. The composition according to p. 7, characterized in that the biologically active molecule is selected from the group consisting of a toxin, drug, and chelate.

9. The composition according to p. 8, characterized in that the said drug is selected from the group consisting of methotrexate, 5-fluorouracil, CIS-platinum and adriamycin.

10. A method of obtaining a composition according to any one of paragraphs.1-9, including the production of monoclonal antibodies, which inbrisbane modified antibodies with less as compared with intact antibody total positive charge, increased antigennegative specificity, low nonspecific binding, and characterized by the rate of clearance in vivo, intermediate between the velocities clearance F(ab)2fragments and intact antibodies of the same type; and the marking of the modified antibodies radioactive isotope, a toxin, a drug, or a chelate with the formation of the labeled modified antibody.

11. The method of localization of the antigen in the mammal with the composition obtained by the method according to p. 10, which includes obtaining labeled modified monoclonal antibodies that have less as compared with intact antibody total positive charge, increased antigennegative specificity, low nonspecific binding, characterized by the rate of clearance in vivo, intermediate between the velocities clearance F(ab)2fragments and intact antibodies of the same type, and contains the registered label; the introduction of the labeled modified antibody to a mammal to bind in vivo antigen labeled with modified antibody and detect the B. p. 11, characterized in that the labeled modified monoclonal antibody tagged with a radioactive isotope.

13. The method according to p. 12, wherein the radioactive isotope is selected from the group consisting of a radioactive isotope of a halogen and technetium.

14. The method according to p. 11, wherein the detection step includes immunoscintigraphy.

15. The method according to p. 14, characterized in that immunoscintigraphy includes the use of gamma cameras.

16. The method according to p. 11, wherein the intact antibody chemically conjugated with heterobifunctional reagent.

 

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