Strain no. 1737/georgia/2000 fmd virus type asia-1 for the manufacture of diagnostic and vaccine preparations

 

The invention relates to the field of veterinary Virology and biotechnology. A new strain of FMD virus type Asia-1 1737/Georgia/2000-DEPT obtained by multiple serial passages in susceptible hetero - and homologous cell cultures. Strain reproducerea in sensitive cell cultures and for 22-24 h of incubation up to 7,2-8,2 Dtcd50/ml. With a massive infection causes JRS after 4 h Preserves the original characteristics when passirovannye in cultures of cells within 10 passages. The resulting strain has a high biological, antigenic and immunogenic activity. The strain can be used for the manufacture of means of specific prophylaxis and diagnosis of FMD type Asia-1, homologous epizootic virus circulating in the Caucasus, Central Asia and the Middle East. 2 Il., 14 table.

The invention relates to the field of veterinary Virology and biotechnology, in particular to the strains of FMDV, which can be used as producers of specific antigens in the manufacture of diagnostic and vaccine preparations.

Foot and mouth disease is an acute contagious viral disease parnok the second cavity, hairless areas of the skin of the head, udder, Corolla, Milpitas cracks and accompanied the traffic violation. For this agent tends to be widely distributed and epizootic period. The disease is accompanied by large losses of milk, meat and other animal products, it is difficult for commercial transactions and economic activities. Long experience shows that with endemic FMD the decline in revenues in dairy and beef cattle by 35-40%.

The virus belongs to the genus of aphthovirus, the family of picornaviruses. He has 7 immunological types and many subtypes (1,2).

The causative agent of FMD has significant antigenic variability of strains within the same serotype, which is revealed in the various time periods and in different areas and depends on the species composition of the susceptible population, its immune status, and many other different factors. In recent years, it is believed that the main cause of antigenic variation is a change in the amino acid sequence of the polypeptides of viral proteins that form the capsid of the virion. Practically such antigenic change can be expressed from neznachitelinoe completely different strains, which require the use of tools for serological diagnostics and specific prophylaxis of disease caused by these strains (2,3).

Known strains of FMD virus type Asia-1, which were registered on the territory of the USSR: Armenia in 1973 and 1984, in Tajikistan in 1974, in Turkmenistan in 1978 and in Georgia in 1984 and 2000, while the isolates in these regions was significantly different from the production strain of FMD virus serological type Asia-1 48 (R was 35-58%).

Used in these areas in a planned manner with preventive and emergency order vaccine from strain of FMD virus serological type Asia-1 48 provided insufficient satisfactory protection of immunized animals and prevent the spread of FMD.

Closest to the proposed invention, the essential features is the production strain of FMD virus serological type Asia-1 48 that is highlighted in the February 1964 on the territory of the Moscow district of the Tajik SSR. The disease caused by this pathogen were recorded sporadically in the USSR until 1966. The specified strain was adapted to the body of mice piglets, 1-2-day-old rabbits and Morse is ub>/ml 5,0 Ig ID50/ml and 5.0 to 6.0 Ig TCD50/ml, respectively. On the antigenic match this strain differed from the reference variant of this type: "Pakistan"1/54 and "Israel"3/63 (4,5).

The strain of FMD virus serological type Asia-1 48 FMD virus has the following characteristics, are presented in table.1.

The shortcomings of the production strain of FMD virus serological type Asia-1 48 are its low biological, antigenic and immunogenic. The vaccine of this strain provides protection against infection with homologous virus. However, in recent years in some countries of the Middle East, Central Asia and Transcaucasia were recorded outbreak of foot and mouth disease caused by strains of the virus that is different from the production strain of FMD virus serological type Asia-1 48 indicators serological relationship.

Thus, in 1999-2000, this pathogen has been recorded in Turkey, Iran, Georgia and other countries.

In ARRIAH laboratory studies aphthous material selected from the sick cattle in Bogdanovsky district of Georgia, was selected strain of FMD virus serological type Asia-1 having antigenic differences from the production strain VV specific prophylaxis and diagnosis of FMD serological type Asia-1, used in Russia and CIS countries, epizootic virus circulating in 2000 in the Caucasus, Central Asia and the Middle East (Georgia, Turkey, Iran).

In this regard, there is a need to get a new production of the epidemic strain of FMD virus serological type Asia-1 to ensure the security of Russia and neighboring countries from this agent.

In the task of creating the present invention was to obtain a new production strain of FMD virus serological type Asia-1, with high biological, antigenic and immunogenic activity in the native form and after inactivation and fabricate diagnostic and vaccine homologous epizootic virus, which appeared in the Caucasus, Central Asia and the Middle East.

The technical result from use of the present invention is to expand the Arsenal of industrial strains of FMD virus serological type Asia-1, with high biological, antigenic and immunogenic activity and suitable for the manufacture of means of specific prophylaxis and diagnosis of FMD type Asia-1, homologically technical result is achieved by obtaining the strain of FMD virus serological type Asia-1 1737/Georgia/2000-DEPT. The strain of FMD virus serological type Asia-1 1737/Georgia/2000-the DEPOT is a new, previously unknown. The original virus to obtain a strain of FMD virus serological type Asia-1 1737/Georgia/2000-DEPT allocated in July 2000 in Bogdanovsky district of Georgia from sick cattle. Production strain of FMD virus serological type Asia-1 1737/Georgia/2000-DEPT obtained by multiple serial passages in susceptible hetero - and homologous cell cultures.

The resulting strain deposited at the Russian state collection of strains of microorganisms used in veterinary medicine and animal husbandry, all-Russian scientific research Institute of control, standardization and certification of veterinary preparations (VGNKI) of the Ministry of agriculture of the Russian Federation on 26 January 2001, under the registration name: the strain of FMD virus serological type Asia-1 1737/Georgia/2000-DEPT.

Compared with the prototype strain of FMD virus serological type Asia-1 1737/Georgia/2000-the DEPOT has a higher biological, antigenic and immunogenic activity in the native form and after inactivation. Experimentally confirmed the possibility of its use for the PA Asia-1 1737/Georgia/2000-DEPT provides obtaining diagnostic products, allow serological diagnosis of virus isolates of FMD serological type Asia-1, causing outbreaks in recent years in the countries of Transcaucasia, Central Asia and the Middle East, and inactivated FMD vaccines that creates the protection of susceptible animals against a specific pathogen.

The invention is explained in graphic materials, where: Fig. 1. presents the amino acid sequence of VP1 protein of a strain of FMD virus serological type Asia-1 1737/Georgia/2000-DEPT; Fig. 2 shows a dendrogram reflecting the phylogenetic relationships of strains of FMD virus serological type Asia-1 1737/Georgia/2000-DEPT with epidemic and vaccine strains of FMD virus serological type Asia-1. The dendrogram based on the comparison of the complete nucleotide sequences of the VP1 gene.

The strain of FMD virus serological type Asia-1 1737/Georgia/2000-DEPT characterized by the following features and properties.

Morphological properties of the Strain of FMD virus serological type Asia-1 1737/Georgia/2000-DEPT belongs to the family Picornaviridae, genus Aphtovirus, serotype Asia-1 and has morphological features that are specific to the causative agent of foot and mouth disease: . Alcova shell consists of 32 capsomeres located in the cubic symmetry.

Antigenic properties of its antigenic properties of the virus strain of FMD serological type Asia-1 1737/Georgia/2000-DEPT belongs to serotype Asia-1. The virus is stable neutralized homologous anticorodal. The virus does not manifest hemagglutinin activity (HA activity). The animals recover in the blood serum of antibodies are formed, which are identified in the RDP, ELISA and PH. When vaccination of cattle vaccine of inactivated virus induces the formation of specific antibodies detected in ELISA, RDP and PH. If hyperimmunization Guinea pigs concentrated inaktivirovannye antigen induces education virousspecificakih antibodies detected in the RAC at a dilution of 1:90-1:180 and in ELISA at a dilution of 1:5000-1:6000.

Method of nucleotide sequencing was determined the primary structure of the VP1 gene of the strain of FMD virus serological type Asia-1 1737/Georgia/2000-DEPT and it is deduced primary structure of a protein VP1 (Fig.1). Comparative analysis of nucleotide sequences showed that the strain of FMD virus serological type Asia-1 1737/Georgia/2000-DEPT differs significantly from the Russian vaccine strain of FMD virus, serologic/1999 (Fig.2). The homology of nucleotide sequences of the strain of FMD virus serological type Asia-1 1737/Georgia/2000 and strains of FMD virus serological type Asia-1 48 and Iran/1999 respectively 86,31% 98,87%.

Closely related strain of FMD virus serological type Asia-1 1737/Georgia/2000-DEPT viruses were isolated in Armenia in 2000 and Georgia in 2001

Thus, phylogenetic analysis showed that strain of FMD virus serological type Asia-1 1737/Georgia/2000-DEPT belongs to a new genetic lines of FMD virus serological type Asia-1. Viruses belonging to this genetic line, circulate on the territory of Central Asia and the Middle East since the second half of the 90-ies.

Antigenic relatedness of strains of FMD virus serological type Asia-1 1737/Georgia/2000-DEPT with a production strain of FMD virus serological type Asia-1 48 and strains, previously isolated in Iran, Pakistan and other countries, studied in RAC. The results of these studies are presented in table. 2. The results are shown in table.2, indicate that the strain of FMD virus serological type Asia-1 1737/Georgia/2000-DEPT identical to the strain of FMD virus type Asia-1/Iran/58/99 and differs from all vishey the ical type Asia-1 1737/Georgia/2000-DEPT exhibits high biological, antigenic and immunogenic activity as in the native form and after inactivation. The strain intended for diagnostic sera and antigen preparations, and also the manufacture of inactivated FMD vaccines. The FMD virus serological type Asia-1 strain 1737/Georgia/2000-DEPT reproducerea in monolayer cultures of primary trypsinization cell culture kidney pig (SP), transplantable cell cultures kidney Siberian mountain goat (PSGC-30) IBRS-2, KSS-21, and for 22-24 h of incubation up to 7,2-8,2 Ig TCD50/ml. With a massive infection causes JRS after 4 hours After 3-5 passages incubation builds up to 6.2 to 8.2 Ig TCD50/ml. Preserves the original characteristics when passirovannye in sensitive biological systems within 10 passages.

Chemo - and geotectonically characteristic Strain of FMD virus serological type Asia-1 1737/Georgia/2000-DEPT is an RNA-containing virus with mol. mass 7106D. Nucleic acid represented by single-stranded linear molecule mol. weight 2,8106D. the virion has a protein shell composed of four basic proteins VP1, VP2, VP3 and VP4. Lipoprotein,5% RNA and 68.5% protein. Firiona RNA is infectious and is involved in the formation of protein precursors in infected cells. Predecessors in turn are split with the formation of more stable structural and non-structural proteins of the virus. Of the 6 non-structural polypeptides that accumulate in infected cells, one (VP66a) is an RNA-dependent RNA polymerase that is involved in the replication of RNA new virions.

The physical properties of the Mass of the virion 8,410-18, the sedimentation Coefficient 146S in the sucrose gradient. Floating density of 1.45 g/ml

Resistance to external factors, the Strain of FMD virus serological type Asia-1 1737/Georgia/2000-DEPT resistant to ether, chloroform, freon, acetone and other organic solvents and detergents. Most stable at pH 7,2-7,6. Changes of pH in acidic and in the alkaline side lead to inactivation of the virus. Sensitive to formaldehyde, UV-irradiation,-exposure to high temperatures.

Additional features and characteristics of Immunogenic activity - immunogene comprising inactivated vaccine.

Reactogenicity - reactogenic properties is not.

The pathogenicity of the pathogenic for parnaka the but-susceptible animals in contact, aerosol and parenteral infection.

Stability - maintains the original biological properties when passirovannye in sensitive biological systems within 10 passages.

Based on the obtained data, it can be argued that the strain of FMD virus serological type Asia-1 1737/Georgia/2000-DEPT on antigenic and immunological spectra is original, in taxonomic relation to new, previously unknown variant of FMD virus type Asia-1.

To reduce its epizootic risk need to provide timely laboratory diagnosis and vaccination emerging foci of the disease, which requires a highly active and specific and antigenic or antibody-based test diagnostics and vaccine derived from the use of this strain as a production.

According to the applicant, the proposed strain meets the conditions of patentability of "novelty" and "inventive step".

The essence of the invention is illustrated by examples of its use.

Example 1
The strain of FMD virus serological type Asia-1 1737/Georgia/2000-DEPT was isolated from field material received in ARRIAH 11.07.2000, in the form of epithelium from the aft cattle, podogrev the FMD type Asia-1. When virus isolation was used complex biological, virological and biochemical methods. These methods included intradermically inoculation material field isolates of cattle (1 passage) and the subsequent adaptation of the virus isolated from a diseased animal to cultures of primary trypsinization and transplantable cell lines. We used cell culture JV, PSGK-30, IBRS-2 and KSS-21. Primary and transplantable cell culture for the production of assay were grown in appropriate culture media in bottles with a capacity of 50 ml, washed from the growth medium and used to infect 10% suspension aphthous material (multiplicity of infection was 1-10 TCD50for a cell), prepared in Hanks solution with 0.5% GLA and antibiotics according to the standard recipe. Removal of microflora and ballast cellular components, the suspension was treated with chloroform (10%). After a 30 minute incubation at +37oWith vials made of 8-10 ml of maintenance medium and incubated at +37oWith until JRS virus. In the presence of JRS (rounding of cells, increasing their optical density, degeneration and separation of the cells from the glass vials were subjected to freeze-thawing, cleaning cleto who was olovely for subsequent passages and research at the RAC for the presence of viral antigen, used set of commercial typespecification sera and sera stored at the Museum of strains ARRIAH.

The results of the adaptation of the virus to different cell cultures are presented in table.3.

The data in the table.3, indicate a good adaptive activity of the strain of FMD virus serological type Asia-1 1737/Georgia/2000-DEPT of used cell cultures.

Isolated using the above methods virus preparation was investigated in RAC with a set of diagnostics on all types of FMD virus, vesicular stomatitis to identify typical facilities and control of purity. The results of typing of the virus in RSK are shown in table.4.

The data in the table.4, indicate that the material examination 1737 identified complimentative antigen of FMDV type Asia-1 at a dilution of 1:2.

Example 2
For hyperimmunization Guinea pigs using antigen from strain of FMD virus serological type Asia-1 1737/Georgia/2000-DEPT reproduced in suspension culture cells KSS-21. Vaccinated suspension clear of ballast impurities by the addition of 0.05% solution of guanidine (phmg). Purified virus concentration the concentrations of 0.01-0.05% of 7,8-8,0.

Inactivated concentrate antigen mixed with an equal volume of oil adjuvant injected Guinea pigs in a volume of 1 ml intramuscularly. After 21 day of immunization and repeat after 10 days, animals bleed. Individual samples of blood serum test sample specificity and activity in the CBD. After that prepare a series by mixing typespecification individual serum samples of the same activity. Strain specificity of serial drug determine in single and duplex reactions with Homo - and heterologous antigens.

After canning sodium azide (1:5000) and incubation at +4oC for 25-30 days the resulting serum is Packed in ampoules of 0.5 to 1 ml.

Was prepared 1 series hyperimmune serum, the characteristics of which are presented in table.5.

The data in the table.5, indicate that the received diagnostic serum, specific activity meets the requirements of GOST 25384-82.

Example 3
To obtain antigen for immunochemical reactions using the strain of FMD virus serological type Asia-1 1737/Georgia/2000-DEPT adapted to the culture of cells of transplantable lines PSGC-30 and KSS-21. To adapt use a virius. The obtained matrix seed is obtained virus used for producing viral material. Contamination of cell cultures and collection of viral material is conducted according to conventional methods. Received vaccinated liquid concentrated 100-fold by adding 8-10% PEG. The obtained concentrate inactivate by heating at +58oC for 40 min, Packed in capsules and dried by sublimation under vacuum.

In this way was prepared 2 series diagnostic antigen, the characteristics of which are given in table.6.

The results of the studies are given in table.6, show that the obtained diagnostic antigens, specific activity which meets the requirements of GOST 25384-82.

Example 4
For the manufacture of the adsorbate-vaccine strain of the virus serological type Asia-1 1737/Georgia/2000-DEPT reproduce in suspension culture cells KSS-21. As a supportive environment using the solution of the Earl without serum with the addition of FGMS, GBX and antibiotics at pH 7.4 and 7.6. Culture of cells infected by a virus from the calculation of 0.01-0.1 TCD50on the cell. The virus cultivation is carried out at a temperature of 35-36,5oC. After 8-10 h of incubation shall count live and dead cells at colouring t the drop in the number of dead cells 90-95% cultivation cease and vaccinated suspension control for sterility and content 146S+75S components. The number 146S+75S components in the suspension should be at least 0.5 μg/ml at the end of the cycle of reproduction of the virus, without temperature control, in vaccinated suspension add 15-20% solution AAAI, acidified with glacial acetic acid to a pH of 8.0 to 8.5. The final concentration AEEI in vaccinated suspension must be equal 0,025-0,05%. Inactivation of the infectivity of the virus is carried out in 12-24 h at 36-37oC and a pH of 7.2 and 7.6 with stirring over 5-6 hours for 3-5 minutes after the inactivation of the antigen suspension is cooled to 4-8oC. To the cooled suspension is added 10% solution of pgmg to the concentration of 0,005-0,007% for ballasted flocculation of impurities and inactivation of possible contaminants. Flocculated ballast impurities is subjected to sedimentation, followed by decantation. The resulting antigen control for avirulence, content virousspecificakih protein and 146S+75S components of the virus, sterility. The necessary concentration of 146S+75S components in pravilnoy dose adsorbed vaccine is obtained by concentration of the antigen by gel GOA.

The estimated amount of gel GOA 3% concentration is added to a cooled suspension of antigen when operating the mixer. Mixing lead within 30 mi is Uspenskii. The final concentration of GOA should be in the range of 1.620,488 mg/ml, p<0,01, n=10, a concentration 146S+75S components of FMD virus at least 2.0 µg/ml Then in the slurry, add an additional 10% solution of saponin to a final concentration of at least 0,075%. The resulting vaccine is filled into glass vials and conduct monitoring sterility according to GOST 28085-89 "biological Preparations. Method of biological control of sterility".

The avirulence and safety of the vaccine tested for 5 heads of cattle, introducing the vaccine first, under the mucous membrane of the tongue at a dose of 2 ml, and then subcutaneously at a dose of 10 ml of monitoring the clinical condition of the animals are 10 days. Avirulent, harmless and sterile vaccine tested for immunogenic activity in cattle or Guinea pigs.

The resulting vaccine is a liquid light amber with a loose white solid sorbent, which is formed on the bottom of the vial during storage and can be easily broken in a homogeneous suspension with shaking.

In table. 7, 8, 9 and 10 shows the results of culturing the strain of FMD virus serological type Asia-1 1737/Georgia/2000-DEPT and strain of FMD virus serological type Asia-1 48 cultivators KS-40 KM-2000, from motorically, that necessitated the selection of the optimal conditions for the cultivation of the virus and multiple doses of infection.

In table.11 shows the results of studies on the effects of inactivation and purification of antigenic material using AAAI and pgmg on the immunogenic components of the strain of FMD virus serological type Asia-1 1737/Georgia/2000-DEPT and strain of FMD virus serological type Asia-1 48. Are given in table.11 evidence suggests that the immunogenic components of a new strain of FMD virus serological type Asia-1 1737/Georgia/2000-DEPT not inferior in resistance to inactivation and purification in the production of immunogenic components of the strain of FMD virus serological type Asia-1 48. Especially important is the fact that in the process of inactivation and purification of viral suspension containing the strain of FMD virus serological type Asia-1 1737/Georgia/2000-the DEPOT, there was no change in the number of 146S+75S components obtained during the reproduction of the virus.

Example 5
Comparative tests were performed on Guinea pigs immunogenic FMD sorbed culture vaccine from strain of FMD virus serological type Asia-1 1737/Georgia/2000-DEPT and FMD sorbed Kul the gene activity of each drug used in 40 Guinea pigs, this 8 heads of each group of animals were control. The vaccine was diluted in phosphate buffer solution in a ratio of 1:3; 1: 9; 1: 27 and 1:81. For each dilution of the vaccine took 8 Guinea pigs. Each group of 8 pigs were kept in a separate cage, which was assigned the label indicating series of the vaccine, its cultivation, the date of commencement of trials and the number of Guinea pigs vaccinated this breeding.

Each vaccine was administered to 4 groups of Guinea pigs intramuscularly in the amount of 2 cm3in the region of the right and left hip on 1 cm3. Unvaccinated groups of Guinea pigs were used to test the activity of the control serotypes of FMD virus.

For vaccinated pigs were observed for 15-17 days. After that, all vaccinated and control animals were infected adapted to them by the FMD virus, homologous vaccine, intradermal in the plantar surface of one of the hind legs at a dose of 104GD50/0.1 cm3. Analysis of infection was performed at 3, 5 and 7 days and was assessed by generalization yasunaga process on the front and one hind legs. Generalization believed education secondary aft on at least one foot, which did not enter the virus. Control HAC less than 7 animals.

Serum from Guinea pigs, selected 30 days after vaccination were tested for tension immunity in the reaction of microneutralization (RMN) and the enzyme-linked immunosorbent assay (ELISA). The results of these studies are presented in table.12, indicate that sera from Guinea pigs immunized with the vaccine of the strain of FMD virus serological type Asia-1 48, ELISA reactions and RMS have higher antibody titers to the homologous strain and less low on epizootic strain of FMD virus serological type Asia-1 1737/Georgia/2000.

Sera from Guinea pigs immunized with the vaccine of the strain of FMD virus serological type Asia-1 1737/Georgia/2000-the DEPOT, on the reactions of ELISA and RMN have a relatively high titers as homologous strain of FMD virus serological type Asia-1 1737//Georgia/2000, and the production strain of FMD virus serological type Asia-1 48.

Thus, the vaccine of the strain of FMD virus serological type Asia-1 1737/Georgia/2000-DEPT may be recommended for vaccination of animals in the areas where this strain is important.

Example 6
Tested the adsorbate-vaccine strain of FMD virus serological type Asia-1 1737/Georgia/200 is Amma virus serological type Asia-1 1737/Georgia/2000-DEPT type induces a sufficient immune response, protect animals from infection control. In addition, it should be noted the increase in the number of BHA in the serum of cattle already on day 10 after vaccination.

Example 7
For the manufacture of emulsion vaccine strain of FMD virus serological type Asia-1 1737/Georgia/2000-DEPT reproduce in suspension culture cells KSS-21, inactivate, clear of ballast impurities and control the resulting antigen to avirulence, content virousspecificakih protein, 146S+75S components of the virus and sterility as described in example 1.

The necessary concentration of 146S+75S components in pravilnoy dose of emulsion vaccine obtained by concentration of the antigen flow ultrafiltration. To do this, use ultrafilter BTU 0.5 to 2. Filtering are under pressure of 1.5 ATM.

The obtained concentrate antigen stored at 4-6oWith until use in the vaccine. Emulsion vaccine is produced by dispersion in colloidal mills concentrate antigen and an oil adjuvant at a ratio of 3:7-1:1, respectively. The result is inactivated emulsion vaccine against FMD type Asia-1, which is a molokopodobnye liquid, insoluble in water. Ve causes a General reaction in the form of a high temperature and has a pronounced immunogenicity for pigs at a dose of 1 ml, for cattle, in the dose of 5 ml and sheep at a dose of 1 ml through 3-21 day after injection. The vaccine is administered to pigs intramuscularly, and cattle and sheep subcutaneously. In previfem volume must contain at least 4 µg 146S+75S components.

Example 8
Emulsion vaccine against FMD serological type Asia-1 is prepared from a strain of FMD virus serological type Asia-1 1737/Georgia/2000-DEPT as described in examples 1, 2, 3. The difference is that the necessary concentration of 146S+75S components in pravilnoy dose of emulsion vaccine obtained by concentration of antigen deposition of PEG-115.

Example 9
Tested emulsion vaccine against FMD type Asia-1, manufactured as described in example 3 and containing of 6.8 µg immunogenic components in pravilnoy dose.

Presented in table.14 data indicate the ability of the proposed vaccine to protect all 15 vaccinated pigs from the control of infection. The titer of neutralizing antibody in the serum on day 21 after vaccination (WPV) was 5.5 log2. Volume pravilnoy doses of the vaccine should contain at least 7 IPD50. Experienced vaccine induced a high level of humoral immunity in pigs and has achieved 40 IPD50in privileges accompanied the present invention, the following cumulative conditions:
a strain of FMD virus serological type Asia-1 1737/Georgia/2000-DEPT embodying the invention, intended for use in agriculture, namely in veterinary Virology and biotechnology;
for the present invention in the form as it is described in the independent claim, confirmed the possibility of its implementation using the steps described in the application or known before the priority date tools and techniques;
a strain of FMD virus serological type Asia-1 1737/Georgia/2000-DEPT received in accordance with the invention, it has high biological, antigenic and immunogenic activity in the native form and after inactivation and suitable for the manufacture of diagnostic and vaccine preparations.

Therefore, the present invention meets the condition of patentability "industrial applicability".

Sources of information taken into account when preparing the description of the invention the application for the grant of a patent of the Russian Federation for the invention of "Strain 1737/Georgia/2000 FMD virus type Asia-1 for the manufacture of diagnostic and vaccine preparations"
1. Rarer X. FMD. / Lane. with it. G. A. Surkova. Ed. and Annot. Kida. wet. Sciences P. C. of Malarze. M: . 20s.

3. Syurin Century. N. and other Viral diseases of animals. M: UNITEMP, 1998. C. 532-548.

4. Instruction for production and control of vaccines monovalent emulsion against FMD type a, type O, type C, type Asia-1 (from virus grown in cell KSS-21). Approved BS of the state Commission of the USSR food and procurement 24.01.91, (prototype).

5. Sobko, A. I. Identification of strains of FMD virus in complex epidemic events. Prof. Diss., 1972.


Claims

The virus strain Aphtae epizooticae, SEM. Picornaviridae, genus Aphtovirus, type Aphtae, type Asia-1, collection VGNKI No. 1737/Georgia/2000-the DEPOT, for the manufacture of diagnostic and vaccine preparations.

 

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