Bikunin person (options) encoding his dna and the expression vector (options)

 

The invention relates to the field of molecular biology and genetic engineering and can be used in medicine. In placental tissue of the person identified protein (bikunin), which contains two domains, characteristic inhibitors of serine proteases family Konitza, and has inhibitory activity against trypsin and plasmin person, comparable to that of Aprotinin, and much more pronounced than that of Aprotinin effect on kallickrein. Defined encoding bikunin human DNA sequence and amino acid sequence of the protein; the proposed expression vectors that provide the possibility of obtaining inhibitor in heterogeneous systems. Synthetic and recombinant method received various fragments and derivatives bikunin, including containing part of the transmembrane domain, which retain at least a portion of the full activity of the native protein. 5 C. and 1 C.p. f-crystals, 24 ill., 11 table.

Description text in facsimile form (see graphic part). T T

Claims

1. Substantially purified protein having inhibitory activity against serine proteases, because native full bikunin (SEQ ID No. 52) or a derived sequence, which is selected from the group consisting of SEQ ID No. 71, SEQ ID No. 70, SEQ ID No. 4, SEQ ID No. 5, SEQ ID No. 6, SEQ ID No. 7, SEQ ID No. 3, SEQ ID No. 50, SEQ ID No. 1 and SEQ ID No. 8, and the presence of at least one interchain disulfide bond.

2. Substantially purified protein containing the sequence of native full-bikunin and at least part of the signal sequence of the polypeptide, and this protein characterized by the amino acid sequence selected from the group consisting of SEQ ID No. 2, SEQ ID No. 45, SEQ ID No. 47, SEQ ID No. 49.

3. Protein under item 1 or 2, characterized in that the protein is glycosylated.

4. The sequence of the nucleic acid encoding the biosynthesis and optionally a secretion protein with inhibitory activity against serine proteases, which is selected from the group of polynucleotide sequences, in accordance with the genetic code determining the amino acid sequence of the protein under item 1 or 2.

5. Recombinant yeast expression vector, characterized by the presence of regulatory elements, provides replication and expression in yeast alien fragment of nucleic acid, which operas is resouses the presence of regulatory elements, provides replication and expression in a cell of an insect alien fragment of nucleic acid, which is operatively linked to the DNA sequence under item 4.

 

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