The method of producing tridecapeptide and pentapeptide to obtain

 

The invention relates to a method of producing tridecapeptide formula I: H-His-Gly-Val-Ser-Gly-His-Gly-Gln-His-Gly-Val-His-Gly-OH and aims to simplify the process and increase the yield of the target product, as well as Pentapeptide formula II: X-His(X)-Gly-Val-Ser(Y)-Gly-OH, which is an intermediate compound in the synthesis. The invention consists in that the solid-phase synthesis of tridecapeptide I is carried out by successive growth of the peptide chain, starting with the C-terminal dipeptidylpeptidase to obtain C-terminal occupationally, which is condensed with a protected N-terminal Pentapeptide formula II: X-His(X)-Gly-Val-Ser(Y)-Gly-OH, where X, Y, a protective group and received tridecapeptide process debateroom agents for the removal of the protective groups and the polymer matrix and produce the final product by HPLC. In addition, another object of the invention is a Pentapeptide of the formula II: X-His(X)-Gly-Val-Ser(Y)-Gly-OH as intermediate compounds for the synthesis of tridecapeptide formula I: H-His-Gly-Val-Ser-Gly-His-Gly-Gln-His-Gly-Val-His-Gly-OH. 2 S. and 1 C.p. f-crystals, 1 table.

Description text in facsimile form (see graphic part).

Claims

1. The method of obtaining tridecoder estlat by successive growth of the peptide chain, since the C-terminal dipeptide, covalently associated with a polymeric matrix, to obtain C-terminal occupationally, which is condensed with the protected Pentapeptide formula II

X-His(X)-Gly-Val-Ser(Y)-Gly-OH,

where X, Y is a protective group,

and received tridecapeptide process debateroom agents for the removal of the protective groups and the polymer matrix and produce the final product by HPLC.

2. The method according to p. 1, characterized in that X represents a tert-butyloxycarbonyl group (BOC), Y - tert-boutelou group (But), a release of protected tridecapeptide is carried out by sequential processing triperoxonane acid; triftormetilfullerenov.

3. Pentapeptide of formula II

X-His(X)-Gly-Val-Ser(Y)-Gly-OH

as intermediate compounds for the synthesis of tridecapeptide formula I

H-His-Gly-Val-Ser-Gly-His-Gly-Gln-His-Gly-Val-His-Gly-OH,

where X, Y is a protective group.

 

Same patents:

The invention relates to the agonist Area-I, comprising the peptide or peptide analogue containing from 15 to 29 amino acid residues, which forms amphipatic-helix in the presence of lipids and which is characterized by the following formula: Z1-X1-X2-X3-X4-X5-X6-X7-X8-X9-X10-X11-X12-X13-X14-X15-X16-X17-X18-X19-X20-X21-X22-X23-Z2,

or its pharmaceutically acceptable salt, values radicals cm

The invention relates to oligopeptides comprising the amino acid sequence of the B-X-X-X-B-X-X-X-J-Tyr, which is Lys or Arg; X represents any amino acid, other than charged aliphatic amino acid or its D-isomer; and J is Gly, Lys or Arg; these amino acids are found in nature L-isomer, D-isomer and norleucine, and where specified Oligopeptide includes other than (a) naturally occurring sequencedomain antigen In human leukocyte (HLA-B) 75-84, (b) a naturally occurring sequence of the transmembrane sequence-chain T-cell receptor of human rights and (in) consistency, which is the mutant with respect to either (a) or (b) having no more than two mutations as an active part of the above oligopeptides; the method of suppressing the activation limfozitah cells, the method of transplantation of donor organs or mammalian cells, methods of inhibiting protein synthesis of inflammatory cytokines by cells capable of producing the specified protein inflammatory cytokine, the method of suppressing an inflammatory response in a mammal, the method of modulation of the activity hemade the

The invention relates to peptides consisting of 16-55 amino acid residues, the peptides comprise at least one of the amino acid sequence: FLCTHIIYS (SEQ ID NO:61), IIYSFANIS (SEQ ID NO: 62), FIKSVPPFL (SEQ ID NO:64), FDGLDLAWL (SEQ ID NO:65), LYPGRRDKQ (SEQ ID NO: 66), YDIAKISQH (SEQ ID NO:67), LDFISIMTY (SEQ ID NO:68), FISIMTYDF (SEQ ID NO: 69), FRGQEDASP (SEQ ID NO: 70), YAVGYMLRL (SEG ID NO:71), MLRLGAPAS (SEQ ID NO:72), LAYYEICDF (SEQ ID NO:73), LRGATVHRT (SEQ ID NO: 74), YLKDRQLAG (SEQ ID NO:75), LAGAMVWAL (SEQ ID NO:76), VWALDLDDF (SEQ ID NO: 77) or LDLDDFQGS (SEQ ID NO:78)

The invention relates to new peptides of General formula 1 (H-a-Lys-d-Trp-Lys-c - Pro-d-Lys-Pro-Trp-e-Arg-NH2where a = -Ile or Lys; b= -Pro - or-Lys-; C, d = -Lys or Trp; e=Arg or Ala, having biocidal activity, which combine higher compared to indolizidines biocidal activity with no damaging effects on blood cells

The invention relates to new peptide-based molecules interleukin-8 General formula

< / BR>
stimulating migration of neutrophils

The invention relates to compounds salts of the polypeptide represented by the formula (I), where a1AND2AND3AND4X, Y and Z have the values given in the description, and the transition metal, which have high antiviral activity against human immunodeficiency virus

The invention relates to a new autoantigen and its derived peptides, their use in the treatment of chronic articular cartilage in autoimmune diseases, pharmaceutical compositions containing these peptides, diagnostic method detection self-reactive T cells in the analyzed sample and the analytical set used in said method

The invention relates to the field of cell biology, in particular to new peptides with the ability to interact with intracellular transduction signal, thereby inhibiting the process of signal transmission, leading to proliferation and cell motility

The invention relates to oligopeptides comprising the amino acid sequence of the B-X-X-X-B-X-X-X-J-Tyr, which is Lys or Arg; X represents any amino acid, other than charged aliphatic amino acid or its D-isomer; and J is Gly, Lys or Arg; these amino acids are found in nature L-isomer, D-isomer and norleucine, and where specified Oligopeptide includes other than (a) naturally occurring sequencedomain antigen In human leukocyte (HLA-B) 75-84, (b) a naturally occurring sequence of the transmembrane sequence-chain T-cell receptor of human rights and (in) consistency, which is the mutant with respect to either (a) or (b) having no more than two mutations as an active part of the above oligopeptides; the method of suppressing the activation limfozitah cells, the method of transplantation of donor organs or mammalian cells, methods of inhibiting protein synthesis of inflammatory cytokines by cells capable of producing the specified protein inflammatory cytokine, the method of suppressing an inflammatory response in a mammal, the method of modulation of the activity hemade the
The invention relates to a group of new protected linear peptides containing the amino acid sequence, which can be used as starting compounds to obtain RGD-containing cyclopeptides, the General formula

R3-Arg-Gly-Asp(OR1)-OR2,

where R1is benzyl or tert-butyl; R2not equal to R1and is selected from the group of tert-butyl; benzyl; 4-methoxybenzyl; 4-nitrobenzyl; diphenylmethyl; 2,2,2-trichloroethyl; 2,2,2-trichloro-1,1-dimethylethyl; allyl; 9-fluorenylmethyl; carboxamidine; substituted 2-sulfonylated type AND-SO2-CH2-CH2- where a is substituted or unsubstituted phenyl or benzyl; R3is a hydrogen atom or a urethane protective group of the B1O-CO-, where1not equal to R1and can take values tert-butyl, benzyl, 4-methoxybenzyl, 9-fluorenylmethyl, 2-(4-nitrophenyloctyl)ethyl; or is a peptidyl containing from one to three amino acid residues; and the peptides, where R3- peptidyl structure E-Z-Y-X-, in which E is a hydrogen atom or a urethane protective group IN2O-CO-, where2not equal to R1and can take values tert-butyl; benzyl; 4-methoxybenzyl; 9-fluorenylmethyl; 2-(4-nitrophen is IN3O-CO-, in which3= R1attached to the omega-amino group; Z can take values Phe or D-Phe

The invention relates to a new compound - peptide of General formula Thr-Gly-Glu-Asn-His-Arg, possessing the biological activity of inducing differentiation and inhibiting cell proliferation in tumors and activity of the tread and the normalizing action on the vital processes of mammalian cells obtained by the method of solid-phase peptide synthesis by sequential growth of the peptide chain and having the following properties: mol

The invention relates to peptides of General formula a-B-C-D-Pro-Gly-Pro-X, where A - O-Met(O), -Met, -Thr, -Ala, -Lys-Gly, -Glu, -Arg, -His, -Phe -,- Tyr, -Trp, B - O, -Glu, -Lys, -Tyr, -Gly, -Arg-Val-Pro; - O, -His, -Pro-Asp-Gly, -Arg, -Tyr, -Val-Phe; D - O-Phe, -His, -Arg, -Lys, -Ala, -Tyr, -Thr, -Pro-Ile; X - O-IlŠµ, highly neurotropic activity (refers to the lack of amino acids), provided that excluded peptides: Glu-His-Phe-Pro-Gly-Pro, Thr-Lys-Pro-Arg-Pro-Gly-Pro, Tyr-Pro-Phe-Pro-Gly-Pro-Ile and Met-Glu-His-Phe-Pro-Gly-Pro

The invention relates to peptides-immunoregulators and their therapeutic use

The invention relates to the derivatives of dolastatin 15 General formula (I) a-b-D-E-F(G)r-(K)s-L, where a, b, D, E, F, G and K are remnants-amino acids and s and r are each independently 0 or 1

The invention relates to compounds of the formula X1-Leu-His-Lys(R1)-Leu-Gln-Thr-Tyr(R2)-Pro-Y, where X1- H-, A1O-CO-, H-Lys(R3)-Leu-Ser-Gln-Glu(B4)-, A1O-CO-Lys(R3)-Leu-Ser-Gln-Glu(B4)-; Y is-OH, -Arg-Thr-Asn-Thr-Gly-Ser-Gly-Thr-Pro-NH2;1there is tert-butyl; R1- protective group of the formula IN the1O-CO - Epsilon-amino group of the Lys residue, R2- protective group of the formula IN the2About-WITH - hydroxyl group of residue Taut, R3- protective group of the formula IN the3O-CO - Epsilon-amino group of the Lys residue IN4- protective group for the gamma-carboxyl group of Glu residue, and1IN2IN3and4may be the same or different and are selected from the range: 2-alkylsulfonates, 2-phenylsulfonyl, 2-(substituted aryl)sulfonylated

The invention relates to the field of chromatography of proteins, can be used in biotechnology for purification and fractionation of enzymes
Up!